公开(公告)号：WO1996022524A1

公开(公告)日：1996-07-25

申请号：PCT/US1995016940

申请日：1995-12-20

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  KEO, Nida ,  SAFARIAN, Zara ,  LIU, Cheng-Ming ,  WANG, HANN-Ping

IPC: G01N27/447

CPC classification number: G01N27/44747

Abstract: A buffer and method useful for the analysis of glycoproteins by capillary zone electrophoresis. The buffer comprises water, a sugar complexing compound, a base compound for adjusting the pH, and a zwitterionic compound. An embodiment of the buffer comprises sodium borate as the complexing compound, sodium hydroxide as the base, and 3-cyclohexylamino-1-propanesulfonic acid as the zwitterionic compound. In the method, a selected glycoprotein is subjected to capillary zone electrophoresis. The proportion or the amount of the glycoprotein is determined by quantitative analysis of the resulting electropherogram.

Abstract translation: 用于通过毛细管区带电泳分析糖蛋白的缓冲液和方法。 缓冲液包括水，糖络合化合物，用于调节pH的碱性化合物和两性离子化合物。 缓冲剂的一个实施方案包括作为络合化合物的硼酸钠，作为碱的氢氧化钠和作为两性离子化合物的3-环己基氨基-1-丙磺酸。 在该方法中，将选择的糖蛋白进行毛细管区带电泳。 通过对所得电泳图的定量分析来确定糖蛋白的比例或量。

公开(公告)号：WO1993010258A1

公开(公告)日：1993-05-27

申请号：PCT/US1992009873

申请日：1992-11-16

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  HSIEH, You-Zung ,  CHEN, Fu-Tai, A. ,  STERNBERG, James, C. ,  KLIEN, Gerald ,  LIU, Cheng-Ming

IPC: C12Q01/00

CPC classification number: C12Q1/007 ,  G01N27/44747

Abstract: Disclosed herein is a methodology for analyzing isoenzymes using capillary zone electrophoresis ('CZE') techniques. Briefly, an isoenzyme-containing sample and a substrate capable of being catalyzed by said isoenzyme into a reaction product are introduced into a capillary column comprising a buffer. Most preferably, the buffer contains the substrate prior to introduction of the sample into such substrate-buffer. CZE separation techniques are applied to the column such that the isoenzymes are separated from each other into discrete zones. The separation techniques are terminated such that product is rapidly generated by the catalytic conversion of substrate by the isoenzymes, and accumulated, within each discrete zone, followed by detection of product. Information regarding the relative distribution of the isoenzymes can be derived from the relative distribution of the product.

公开(公告)号：WO1995010041A1

公开(公告)日：1995-04-13

申请号：PCT/US1994010402

申请日：1994-09-15

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  WANG, Hann-Ping ,  LIU, Cheng-Ming

IPC: G01N27/447

CPC classification number: G01N27/44726 ,  G01N27/44743

Abstract: A method of quantitating proteins in complex samples using capillary electrophoresis can be used both to determine the concentration of a protein in a sample and to determine total protein concentration in the sample. In general, the method of determining the concentration of a marker protein comprises: (1) adding a known quantity of an internal standard compound to a sample containing at least one protein, the internal standard compound selected from the group consisting of benzoic acid substituted with at least one halogen, producing a detector signal in relation to its concentration, and being capable of electrophoretic separation from the protein; (2) subjecting the sample and the internal standard compound to capillary electrophoresis to separate the protein and the internal standard compound from each other and from other components in the sample; (3) measuring the detector signal produced by the internal standard compound and a detector signal produced by the protein to determine a ratio of protein signal to internal standard signal; and (4) determining the concentration of the protein in the sample from a standard curve of protein concentration versus the ratio of protein signal to internal standard signal. A typical protein measurable by the method is albumin. Typically, measurements are made at 214 nm and the internal standard compound is 2,4-dichlorobenzoic acid.

Abstract translation: 使用毛细管电泳定量复杂样品中的蛋白质的方法可以用于确定样品中蛋白质的浓度并确定样品中的总蛋白质浓度。 通常，确定标记蛋白浓度的方法包括：（1）向含有至少一种蛋白质的样品中加入已知量的内标化合物，所述内标化合物选自由 至少一个卤素，产生与其浓度相关的检测器信号，并且能够与蛋白质电泳分离; （2）对样品和内标化合物进行毛细管电泳，将蛋白质和内标化合物与样品中的其他成分分离; （3）测量由内标化合物产生的检测器信号和由蛋白质产生的检测器信号，以确定蛋白质信号与内标信号的比率; 和（4）从蛋白质浓度的标准曲线与蛋白质信号与内标信号的比率确定样品中蛋白质的浓度。 通过该方法测量的典型蛋白质是白蛋白。 通常，测量在214nm，内标化合物是2,4-二氯苯甲酸。

公开(公告)号：WO1995002182A1

公开(公告)日：1995-01-19

申请号：PCT/US1994005631

申请日：1994-05-18

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  LIU, Cheng-Ming ,  WANG, Hann-Ping

IPC: G01N33/483

CPC classification number: G01N30/14 ,  G01N33/491 ,  G01N33/493 ,  Y10S436/828 ,  Y10T436/25125 ,  Y10T436/255

Abstract: Processes are provided for pretreating body fluid compositions and subsequently analyzing the pretreated body fluid compositions for analytes of interest. Processes for pretreating the compositions include providing size exclusion gel having a molecular weight fractionation range or a molecular weight exclusion such that the size exclusion gel is capable of excluding or fractionating the analytes of interest, and then causing the composition to contact the size exclusion gel in order to separate the analytes from low molecular weight composition components which interfere with the separation and analysis of the analytes of interest. Processes for analyzing pretreated compositions include electrophoretic methods such as capillary zone electrophoresis which involve the separation and detection of analytes of interest.

Abstract translation: 提供了用于预处理体液组合物并随后分析预处理的体液组合物用于所关注的分析物的方法。 用于预处理组合物的方法包括提供具有分子量分馏范围或分子量排除的尺寸排阻凝胶，使得尺寸排阻凝胶能够排除或分级分析所关注的分析物，然后使组合物与尺寸排阻凝胶接触 将分析物与低分子量组成成分分离开来，这些成分干扰了目的分析物的分离和分析。 用于分析预处理组合物的方法包括电泳方法，例如涉及分离和检测感兴趣分析物的毛细管区带电泳。

公开(公告)号：EP0572604B1

公开(公告)日：1997-05-07

申请号：EP92925261.7

申请日：1992-11-16

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： HSIEH, You-Zung ,  CHEN, Fu-Tai, A. ,  STERNBERG, James, C. ,  KLIEN, Gerald ,  LIU, Cheng-Ming

IPC: C12Q1/00 ,  G01N27/447

CPC classification number: C12Q1/007 ,  G01N27/44747

Abstract: Disclosed herein is a methodology for analyzing isoenzymes using capillary zone electrophoresis ('CZE') techniques. Briefly, an isoenzyme-containing sample and a substrate capable of being catalyzed by said isoenzyme into a reaction product are introduced into a capillary column comprising a buffer. Most preferably, the buffer contains the substrate prior to introduction of the sample into such substrate-buffer. CZE separation techniques are applied to the column such that the isoenzymes are separated from each other into discrete zones. The separation techniques are terminated such that product is rapidly generated by the catalytic conversion of substrate by the isoenzymes, and accumulated, within each discrete zone, followed by detection of product. Information regarding the relative distribution of the isoenzymes can be derived from the relative distribution of the product.

公开(公告)号：EP0672249A1

公开(公告)日：1995-09-20

申请号：EP94929210.0

申请日：1994-09-15

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： WANG, Hann-Ping ,  LIU, Cheng-Ming

IPC: G01N33 ,  G01N21 ,  G01N27

CPC classification number: G01N27/44726 ,  G01N27/44743

Abstract: A method of quantitating proteins in complex samples using capillary electrophoresis can be used both to determine the concentration of a protein in a sample and to determine total protein concentration in the sample. In general, the method of determining the concentration of a marker protein comprises: (1) adding a known quantity of an internal standard compound to a sample containing at least one protein, the internal standard compound selected from the group consisting of benzoic acid substituted with at least one halogen, producing a detector signal in relation to its concentration, and being capable of electrophoretic separation from the protein; (2) subjecting the sample and the internal standard compound to capillary electrophoresis to separate the protein and the internal standard compound from each other and from other components in the sample; (3) measuring the detector signal produced by the internal standard compound and a detector signal produced by the protein to determine a ratio of protein signal to internal standard signal; and (4) determining the concentration of the protein in the sample from a standard curve of protein concentration versus the ratio of protein signal to internal standard signal. A typical protein measurable by the method is albumin. Typically, measurements are made at 214 nm and the internal standard compound is 2,4-dichlorobenzoic acid.

公开(公告)号：EP0750745A1

公开(公告)日：1997-01-02

申请号：EP95944411.0

申请日：1995-12-20

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： KEO, Nida ,  SAFARIAN, Zara ,  LIU, Cheng-Ming ,  WANG, HANN-Ping

IPC: G01N33 ,  G01N27

CPC classification number: G01N27/44747

Abstract: A buffer and method useful for the analysis of glycoproteins by capillary zone electrophoresis. The buffer comprises water, a sugar complexing compound, a base compound for adjusting the pH, and a zwitterionic compound. An embodiment of the buffer comprises sodium borate as the complexing compound, sodium hydroxide as the base, and 3-cyclohexylamino-1-propanesulfonic acid as the zwitterionic compound. In the method, a selected glycoprotein is subjected to capillary zone electrophoresis. The proportion or the amount of the glycoprotein is determined by quantitative analysis of the resulting electropherogram.

Abstract translation: 用于通过毛细管区带电泳分析糖蛋白的缓冲液和方法。 该缓冲液包含水，糖络合化合物，用于调节pH的碱化合物和两性离子化合物。 缓冲剂的一个实施方案包含作为络合化合物的硼酸钠，作为碱的氢氧化钠和作为两性离子化合物的3-环己基氨基-1-丙烷磺酸。 在该方法中，将选择的糖蛋白进行毛细管区带电泳。 通过定量分析所得电泳图确定糖蛋白的比例或量。

公开(公告)号：EP0572604A1

公开(公告)日：1993-12-08

申请号：EP92925261.0

申请日：1992-11-16

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： HSIEH, You-Zung ,  CHEN, Fu-Tai, A. ,  STERNBERG, James, C. ,  KLIEN, Gerald ,  LIU, Cheng-Ming

IPC: C12Q1 ,  G01N27

CPC classification number: C12Q1/007 ,  G01N27/44747

Abstract: Procédé d'analyse d'isoenzymes utilisant des techniques d'électrophorèse à zone capillaire ("CZE"). Un échantillon contenant une isoenzyme et un substrat pouvant être catalysé par ladite isoenzyme en un produit de réaction sont introduits dans une colonne capillaire comprenant un tampon. Idéalement, le tampon contient le substrat avant l'introduction de l'échantillon. On applique à la colonne des techniques de séparation CZE, afin de séparer les isoenzymes en des zones discrètes. A la fin de la séparation, le produit est rapidement généré par la conversion catalytique du substrat par les isoenzymes, et accumulé dans chaque zone discrète. Le produit est ensuite détecté. On peut dériver les informations sur la répartition relative des isoenzymes à partir de la distribution relative du produit.

公开(公告)号：EP0690988A1

公开(公告)日：1996-01-10

申请号：EP95908505.0

申请日：1995-01-17

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： WANG, Hann-Ping ,  LIU, Cheng-Ming

IPC: G01N27 ,  G01N33

CPC classification number: G01N33/6854 ,  G01N27/44726 ,  G01N33/561

Abstract: A method for classifying and typing M-proteins present in a sample of serum, etc. of an individual is disclosed. The method employs an on-capillary electrophoretic immunosubtractive method to analyze and classify identified M-proteins.

公开(公告)号：EP0659274A1

公开(公告)日：1995-06-28

申请号：EP94921199.0

申请日：1994-05-18

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： LIU, Cheng-Ming ,  WANG, Hann-Ping

IPC: B01J20 ,  C07K1 ,  G01N1 ,  G01N27 ,  G01N30 ,  G01N33

CPC classification number: G01N30/14 ,  G01N33/491 ,  G01N33/493 ,  Y10S436/828 ,  Y10T436/25125 ,  Y10T436/255

Abstract: Processes are provided for pretreating body fluid compositions and subsequently analyzing the pretreated body fluid compositions for analytes of interest. Processes for pretreating the compositions include providing size exclusion gel having a molecular weight fractionation range or a molecular weight exclusion such that the size exclusion gel is capable of excluding or fractionating the analytes of interest, and then causing the composition to contact the size exclusion gel in order to separate the analytes from low molecular weight composition components which interfere with the separation and analysis of the analytes of interest. Processes for analyzing pretreated compositions include electrophoretic methods such as capillary zone electrophoresis which involve the separation and detection of analytes of interest.