公开(公告)号：KR1020000019418A

公开(公告)日：2000-04-06

申请号：KR1019980037509

申请日：1998-09-11

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 현방훈 ,  송재영 ,  안동준 ,  박종현 ,  차상호 ,  안수환

IPC: C12N15/63 ,  C12N15/86

CPC classification number: C12N15/86 ,  A61K39/12 ,  C12N7/00

Abstract: PURPOSE: Recombinant porcine Aujesky's disease virus provides safe vector vaccine against porcine diseases and can be used to produce effective vaccine against other diseases if antigen of other virus is introduced in it. CONSTITUTION: Recombinant porcine Aujesky's disease virus is useful to prepared a vaccine vector. The vector is constructed by; deletion of K gene; insertion of IL-2 gene; deletion of gI gene; insertion of beta-galactosidase gene as a maker gene; and insertion of foreign gene coding antigen for virus pathogen. Thereby, immunity is improved.

Abstract translation: 目的：重组猪Aujesky病病毒提供安全的针对猪疾病的载体疫苗，如果其中引入其他病毒的抗原，可用于产生针对其他疾病的有效疫苗。 构成：重组猪Aujesky病病毒可用于制备疫苗载体。 矢量由...构建; 缺失K基因; 插入IL-2基因; 缺失gI基因; 插入β-半乳糖苷酶基因作为制造者基因; 并插入外源基因编码抗原用于病毒病原体。 从而，免疫力得到改善。

公开(公告)号：KR1020000003768A

公开(公告)日：2000-01-25

申请号：KR1019980025040

申请日：1998-06-29

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 현방훈 ,  송재영 ,  안동준 ,  박종현 ,  차상호 ,  안수환

IPC: C12N15/86 ,  C12N15/33

CPC classification number: C12N15/861 ,  C07K14/005

Abstract: PURPOSE: Provided are a fluorescent vector, which is used for the production of vaccine of porcine Aujesky's disease and a method for selecting recombination virus using the same. CONSTITUTION: A fluorescent vector pHAVE3 is prepared by amplifying pVIII gene corresponding to 5' region of E3 gene of human Adenovirus type II, a partial sequence(27100-28811bp) having 1,712base, before open reading frame of E3 gene being 19kd, a partial sequence(30,851-32,760bp) having 1,910base, after E3-2 polyadenylation signal corresponding to 3' region of E3 gene; cloning them into SpeI of plasmid pGEMT; and inserting BamH I restriction site

Abstract translation: 目的：提供一种荧光载体，其用于生产猪Aujesky病疫苗，以及使用该载体选择重组病毒的方法。 构成：荧光载体pHAVE3通过扩增对应于人腺病毒II型E3基因的5'区域的pVIII基因，其具有1,712碱基的部分序列（27100-28811bp），E3基因的开放阅读框为19kd，部分 序列（30,851-32760bp）具有1,910个碱基，E3-2多聚腺苷酸化信号对应于E3基因的3'区域; 将其克隆入SpeI质粒pGEMT; 并插入BamH I限制位点