公开(公告)号：WO2007146154A1

公开(公告)日：2007-12-21

申请号：PCT/US2007/013553

申请日：2007-06-06

Applicant: GEN-PROBE INCORPORATED ,  BECKER, Michael, M. ,  LIVEZEY, Kristin, W. ,  LAM, Wai-Chung

Inventor： BECKER, Michael, M. ,  LIVEZEY, Kristin, W. ,  LAM, Wai-Chung

IPC: C12Q1/68

CPC classification number: C12Q1/6853 ,  C12Q1/6848 ,  C12Q1/6865 ,  C12Q2525/301 ,  C12Q2525/186 ,  C12Q2525/155 ,  C12Q2525/161

Abstract: The present invention provides nucleic acid amplification methods that desirably reduce or eliminate false positive amplification signals resulting from contaminating biological material, e.g., nucleic acid, that may be present in one or more reagents used in an amplification reaction and/or that may be present in the environment in which an amplification reaction is performed. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in amplification reactions, and the environment in which an amplification reaction is performed, are free of bacterial or other nucleic acid contamination that may yield false positive results.

Abstract translation: 本发明提供了核酸扩增方法，其期望地减少或消除由可能存在于扩增反应中使用的一种或多种试剂中的生物材料例如核酸引起的假阳性扩增信号和/或可存在于 进行扩增反应的环境。 本发明提供了进一步的优点，即需要比常规所需的更少的纯化和/或不育努力，以确保用于扩增反应的酶和其它试剂以及进行扩增反应的环境中没有细菌或其它 可能产生假阳性结果的核酸污染。

公开(公告)号：WO2006026388A3

公开(公告)日：2007-01-18

申请号：PCT/US2005030329

申请日：2005-08-26

Applicant: GEN PROBE INC ,  BECKER MICHAEL M ,  LAM WAI-CHUNG ,  LIVEZEY KRISTIN W ,  BRENTANO STEVEN T ,  KOLK DANIEL P ,  SCHRODER ASTRID R W

Inventor： BECKER MICHAEL M ,  LAM WAI-CHUNG ,  LIVEZEY KRISTIN W ,  BRENTANO STEVEN T ,  KOLK DANIEL P ,  SCHRODER ASTRID R W

IPC: C12P19/34 ,  C07H21/04

CPC classification number: C12P19/34 ,  C12Q1/6865 ,  C12Q2537/163 ,  C12Q2521/325 ,  C12Q2521/107 ,  C12Q2525/186 ,  C12Q2533/101 ,  C12Q2525/143

Abstract: The present invention is directed to novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic (i.e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, the present invention discloses a method of nucleic acid amplification which is robust and efficient, while reducing the appearance of side-products. The method uses only one primer, the "priming oligonucleotide," a 3'blocked promoter oligonucleotide and optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or eliminating the formation of side-products. The method of the present invention minimizes or eliminates the emergence of side-products, thus providing a high level of specificity. Furthermore, the appearance of side-products can complicate the analysis of the amplification reaction by various molecular detection techniques. The present invention minimizes or eliminates this problem, thus providing an enhanced level of sensitivity.

Abstract translation: 本发明涉及合成目标核酸序列的多个拷贝的新型方法，所述目标核酸序列是自动催化的（即，能够自动循环而不需要修饰诸如温度，pH或离子强度的反应条件，并且使用一种 循环在下一个）。 特别地，本发明公开了一种鲁棒有效的核酸扩增方法，同时减少副产物的外观。 该方法仅使用一种引物“引物寡核苷酸”，3'封闭的启动子寡核苷酸和任选的终止引物延伸反应的手段，以体外扩增RNA或DNA分子，同时减少或消除副产物的形成 。 本发明的方法使副产物的出现最小化或消除，从而提供高水平的特异性。 此外，副产物的出现可能通过各种分子检测技术使扩增反应的分析复杂化。 本发明使这个问题最小化或消除了这一点，从而提供了增强的灵敏度。

公开(公告)号：WO2006121888A3

公开(公告)日：2006-11-16

申请号：PCT/US2006/017472

申请日：2006-05-05

Applicant: GEN-PROBE INCORPORATED ,  POLLNER, Reinhold B. ,  BECKER, Michael M. ,  MAJLESSI, Mehrdad R.

Inventor： POLLNER, Reinhold B. ,  BECKER, Michael M. ,  MAJLESSI, Mehrdad R.

IPC: C12Q1/68

Abstract: Methods for efficiently capturing a target nucleic acid from a sample by using a mixture that contains a capture probe specific for the target nucleic acid, the target nucleic acid, and a denaturant chemical, which mixture is incubated at elevated temperature for a short time, are disclosed. Compositions that include a capture probe that specifically binds to a target nucleic acid and a denaturant chemical, which when mixed with the target nucleic acid and incubated at elevated temperature for a short time, promote efficient hybridization of the capture probe and target nucleic acid are disclosed.

公开(公告)号：WO0116371A3

公开(公告)日：2002-07-11

申请号：PCT/US0023422

申请日：2000-08-25

Applicant: GEN PROBE INC ,  FRIEDENBERG MATTHEW C ,  BECKER MICHAEL M ,  MAJLESSI MEHRDAD ,  RUSSELL JAMES ,  WEISBURG WILLIAM G

Inventor： FRIEDENBERG MATTHEW C ,  BECKER MICHAEL M ,  MAJLESSI MEHRDAD ,  RUSSELL JAMES ,  WEISBURG WILLIAM G

IPC: C12Q1/68 ,  C07H21/00 ,  G01N21/64

CPC classification number: C12Q1/68

Abstract: Methods for detecting and identifying individual nucleic acid base phosphoramidites in a sample solution by matching fluorescent excitation and/or emission spectra to similar spectra of known phosphoramidites are disclosed. Methods of determining the accuracy of nucleic acid synthesis by analyzing fluorescent spectra of samples (influent, effluent, reaction mixtures) associated with individual coupling steps that use nucleic acid base phosphoramidites andidentifying the phosphoramidite contained in each sample are disclosed.

Abstract translation: 公开了通过将荧光激发和/或发射光谱与已知亚磷酰胺的类似光谱匹配来检测和鉴定样品溶液中的各个核酸碱基亚磷酰胺的方法。 公开了通过分析与使用核酸碱基亚磷酰胺和识别每个样品中所含的亚磷酰胺的各个偶联步骤相关的样品（流入物，流出物，反应混合物）的荧光光谱来确定核酸合成的准确性的方法。

公开(公告)号：WO2010151566A8

公开(公告)日：2011-07-21

申请号：PCT/US2010039592

申请日：2010-06-23

Applicant: GEN PROBE INC ,  KAPLAN SHANNON K ,  LIVEZEY KRISTIN W ,  BECKER MICHAEL M ,  HOGAN JAMES D

Inventor： KAPLAN SHANNON K ,  LIVEZEY KRISTIN W ,  BECKER MICHAEL M ,  HOGAN JAMES D

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/16

Abstract: Compositions, reaction mixtures, kits and methods used in amplifying and detecting nucleic acids from various species of the class Mollicutes. In certain aspects and embodiments, particular regions of the 23S rRNA or gene encoding said rRNA have been identified as preferred targets for nucleic acid amplification reactions of a sample suspected containing at least one species of Mollicutes. Some oligomers comprise tag regions. Some oligomers comprise target closing regions, promoter sequences, and/or binding moieties, such as homopolymeric and heteropolymeric nucleotide sequences. Samples can be from any source suspected of containing a species of the class Mollicutes. Preferred sample sources include bioreactors, cell lines, cell culture wares and pharmaceutical manufacturing wares, to name a few.

Abstract translation: 用于扩增和检测来自Mollicutes类的各种物种的核酸的组合物，反应混合物，试剂盒和方法。 在某些方面和实施方案中，编码所述rRNA的23S rRNA或基因的特定区域已被鉴定为怀疑含有至少一种Mollicutes的样品的核酸扩增反应的优选靶标。 一些低聚物包括标签区。 一些寡聚体包含靶闭合区，启动子序列和/或结合部分，例如均聚和杂聚核苷酸序列。 样品可以来自怀疑含有Mollicutes类物种的任何来源。 优选的样品来源包括生物反应器，细胞系，细胞培养物和药物制造商品等等。

公开(公告)号：WO2009140374A3

公开(公告)日：2010-01-07

申请号：PCT/US2009043775

申请日：2009-05-13

Applicant: GEN PROBE INC ,  BECKER MICHAEL M ,  LIVEZEY KRISTIN W

Inventor： BECKER MICHAEL M ,  LIVEZEY KRISTIN W

IPC: C12Q1/68 ,  G01N33/53

CPC classification number: C12Q1/6816 ,  C12Q1/6832 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2565/107 ,  C12Q2563/179 ,  C12Q2527/101 ,  C12Q2537/1373

Abstract: Disclosed are compositions, kits and methods for a selective hybridization and capture of a specific target nucleic acid in a heterogeneous mixture of nucleic acids The target nucleic acids are then used in subsequent analysis The method offers the advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure a suitable environment for using enzymes and other reagents in subsequent analysis

Abstract translation: 公开了用于在核酸的非均质混合物中选择性杂交和捕获特异性靶核酸的组合物，试剂盒和方法。然后将靶核酸用于随后的分析。该方法提供了要求较不严格的纯化和/或不育的优点 为了确保在随后的分析中使用酶和其它试剂的合适环境，传统上所需要的努力

公开(公告)号：WO2009140374A2

公开(公告)日：2009-11-19

申请号：PCT/US2009/043775

申请日：2009-05-13

Applicant: GEN-PROBE INCORPORATED ,  BECKER, Michael, M. ,  LIVEZEY, Kristin, W.

Inventor： BECKER, Michael, M. ,  LIVEZEY, Kristin, W.

IPC: C12Q1/68 ,  C07H21/00 ,  G01N33/566

CPC classification number: C12Q1/6816 ,  C12Q1/6832 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2565/107 ,  C12Q2563/179 ,  C12Q2527/101 ,  C12Q2537/1373

Abstract: The present invention provides compositions, kits and methods for the selective hybridization and capture of a specific target nucleic acid. The specific target nucleic acid may be present in a heterogeneous mixture of nucleic acids. Selective hybridization and capture provides a target nucleic acid that is substantially free of non-target and/or contaminating nucleic acids. Target nucleic acids that have been selectively hybridized and captured using the current invention are then used in subsequent analysis, wherein the presence of non-target and/or contaminating nucleic acids that interfere with said subsequent analysis have been substantially reduced or eliminated, thereby providing improved analysis results. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in subsequent analysis, or present in the environment in which an assay is performed, are free of bacterial or other contaminating nucleic acids.

Abstract translation: 本发明提供用于选择性杂交和捕获特异性靶核酸的组合物，试剂盒和方法。 特异性靶核酸可以存在于核酸的非均相混合物中。 选择性杂交和捕获提供基本上不含非靶和/或污染性核酸的靶核酸。 然后使用本发明选择性杂交和捕获的目标核酸用于随后的分析中，其中干扰所述后续分析的非目标和/或污染性核酸的存在已被大大减少或消除，从而提供改进的 分析结果。 本发明提供了进一步的优点，其需要比常规所需的更少的纯化和/或不育努力，以确保用于后续分析中或存在于进行测定的环境中的酶和其它试剂不含细菌或 其他污染性核酸。

公开(公告)号：WO2007030505A1

公开(公告)日：2007-03-15

申请号：PCT/US2006/034663

申请日：2006-09-06

Applicant: GEN-PROBE INCORPORATED ,  BECKER, Michael, M. ,  LIVEZEY, Kristin, W.

Inventor： BECKER, Michael, M. ,  LIVEZEY, Kristin, W.

IPC: C12Q1/68

CPC classification number: C12Q1/6844 ,  C12P19/34 ,  C12Q1/6853 ,  C12Q2527/101 ,  C12Q2537/155 ,  C12Q2525/161 ,  C12Q2525/179 ,  C12Q2537/1376

Abstract: Methods and compositions are described for isothermal nucleic acid amplification of a nucleic acid template strand by using an oligonucleotide primer that includes an AT-rich nucleotide sequence and a polymerase having strand displacement activity.

Abstract translation: 描述了通过使用包含富含AT的核苷酸序列的寡核苷酸引物和具有链置换活性的聚合酶的核酸模板链的等温核酸扩增的方法和组合物。

公开(公告)号：WO0001850A3

公开(公告)日：2000-04-06

申请号：PCT/US9915098

申请日：1999-07-01

Applicant: GEN PROBE INC ,  BECKER MICHAEL M ,  SCHROTH GARY

Inventor： BECKER MICHAEL M ,  SCHROTH GARY

IPC: C12N15/09 ,  C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q2563/107 ,  C12Q2525/197 ,  C12Q2525/161

Abstract: The present invention features "molecular torches" and the use of molecular torches for detecting the presence of a target nucleic acid sequence. Molecular torches contain a target binding domain, a target closing domain, and a joining region. The target binding domain is biased towards the target sequence such that the target binding domain forms a more stable hybrid with the target sequence than with the target closing domain under the same hybridization conditions. The joining region facilitates the formation or maintenance of a closed torch.

Abstract translation: 本发明的特征在于“分子炬”和使用分子炬来检测目标核酸序列的存在。 分子火炬包含目标结合域，靶闭合结构域和连接区。 靶结合结构域偏向靶序列，使得靶结合结构域在相同杂交条件下与靶序列形成比靶终闭结构域更稳定的杂交体。 接合区域有助于形成或维护闭合的割炬。

公开(公告)号：WO2010151566A1

公开(公告)日：2010-12-29

申请号：PCT/US2010/039592

申请日：2010-06-23

Applicant: GEN-PROBE INCORPORATED ,  KAPLAN, Shannon, K. ,  LIVEZEY, Kristin, W. ,  BECKER, Michael, M. ,  HOGAN, James, D.

Inventor： KAPLAN, Shannon, K. ,  LIVEZEY, Kristin, W. ,  BECKER, Michael, M. ,  HOGAN, James, D.

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/16

Abstract: Compositions, reaction mixtures, kits and methods used in amplifying and detecting nucleic acids from various species of the class Mollicutes. In certain aspects and embodiments, particular regions of the 23S rRNA or gene encoding said rRNA have been identified as preferred targets for nucleic acid amplification reactions of a sample suspected containing at least one species of Mollicutes. Some oligomers comprise tag regions. Some oligomers comprise target closing regions, promoter sequences, and/or binding moieties, such as homopolymeric and heteropolymeric nucleotide sequences. Samples can be from any source suspected of containing a species of the class Mollicutes. Preferred sample sources include bioreactors, cell lines, cell culture wares and pharmaceutical manufacturing wares, to name a few.

Abstract translation: 用于扩增和检测来自Mollicutes类的各种物种的核酸的组合物，反应混合物，试剂盒和方法。 在某些方面和实施方案中，编码所述rRNA的23S rRNA或基因的特定区域已被鉴定为怀疑含有至少一种Mollicutes的样品的核酸扩增反应的优选靶标。 一些低聚物包括标签区。 一些寡聚体包含靶闭合区，启动子序列和/或结合部分，例如均聚和杂聚核苷酸序列。 样品可以来自怀疑含有Mollicutes类物种的任何来源。 优选的样品来源包括生物反应器，细胞系，细胞培养物和药物制造商品等等。