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    DETECTION OF ANTIBIOTIC-RESISTANT MICROORGANISMS
    3.
    发明申请
    DETECTION OF ANTIBIOTIC-RESISTANT MICROORGANISMS 审中-公开
    抗生素耐药微生物的检测

    公开(公告)号：WO2009086218A3

    公开(公告)日：2009-09-03

    申请号：PCT/US2008087859

    申请日：2008-12-19

    Applicant: GEN PROBE INC ,  BECKER MICHAEL M ,  GAO KUI ,  LAM WAI-CHUNG

    Inventor： BECKER MICHAEL M ,  GAO KUI ,  LAM WAI-CHUNG

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q2600/16 ,  C12Q2545/114 ,  C12Q2545/101 ,  C12Q2531/113

    Abstract: Method of detecting methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) in a nucleic acid coamplification assay. The invention advantageously reduces the incidence of false-positive MRSA determinations in real-time assays by requiring satisfaction of a threshold criterion that excludes certain co-infections from the MRSA determination. The invention further provides for determination of MSSA, even when the MSSA is present in combination with methicillin-resistant coagulase-negative (MR-CoNS) bacteria at high or low levels.

    Abstract translation: 在核酸共扩增测定中检测耐甲氧西林金黄色葡萄球菌（MRSA）和甲氧西林敏感性金黄色葡萄球菌（MSSA）的方法。 本发明有利地通过要求满足从MRSA测定中排除某些共感染的阈值标准来降低实时分析中假阳性MRSA测定的发生率。 本发明还提供MSSA的测定，即使MSSA与高水平或低水平的耐甲氧西林凝固酶阴性（MR-CoNS）细菌组合存在。

    DETECTION OF ANTIBIOTIC-RESISTANT MICROORGANISMS
    4.
    发明申请
    DETECTION OF ANTIBIOTIC-RESISTANT MICROORGANISMS 审中-公开
    检测抗生素微生物

    公开(公告)号：WO2009086218A2

    公开(公告)日：2009-07-09

    申请号：PCT/US2008/087859

    申请日：2008-12-19

    Applicant: GEN-PROBE INCORPORATED ,  BECKER, Michael, M. ,  GAO, Kui ,  LAM, Wai-Chung

    Inventor： BECKER, Michael, M. ,  GAO, Kui ,  LAM, Wai-Chung

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q2600/16 ,  C12Q2545/114 ,  C12Q2545/101 ,  C12Q2531/113

    Abstract: Method of detecting methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) in a nucleic acid coamplification assay. The invention advantageously reduces the incidence of false-positive MRSA determinations in real-time assays by requiring satisfaction of a threshold criterion that excludes certain co-infections from the MRSA determination. The invention further provides for determination of MSSA, even when the MSSA is present in combination with methicillin-resistant coagulase-negative (MR-CoNS) bacteria at high or low levels.

    Abstract translation: 在核酸共扩增测定中检测耐甲氧西林的金黄色葡萄球菌（MRSA）和甲氧西林敏感的金黄色葡萄球菌（MSSA）的方法。 本发明通过要求满足从MRSA测定中排除某些共同感染的阈值标准而有利地降低了实时测定中假阳性MRSA测定的发生率。 即使当MSSA以高或低水平与耐甲氧西林凝固酶阴性（MR-CoNS）细菌联合存在时，本发明还提供了MSSA的测定。

    METHODS OF NUCLEIC ACID TARGET CAPTURE
    6.
    发明申请
    METHODS OF NUCLEIC ACID TARGET CAPTURE 审中-公开
    核酸目标捕获方法

    公开(公告)号：WO2006121888A2

    公开(公告)日：2006-11-16

    申请号：PCT/US2006017472

    申请日：2006-05-05

    Applicant: GEN PROBE INC ,  POLLNER REINHOLD B ,  BECKER MICHAEL M ,  MAJLESSI MEHRDAD R

    Inventor： POLLNER REINHOLD B ,  BECKER MICHAEL M ,  MAJLESSI MEHRDAD R

    IPC: C12Q1/68

    CPC classification number: C12Q1/6813 ,  C12Q1/6834 ,  Y10T436/143333 ,  C12Q2537/125 ,  C12Q2523/113 ,  C12Q2565/519 ,  C12Q2527/101

    Abstract: Methods for efficiently capturing a target nucleic acid from a sample by using a mixture that contains a capture probe specific for the target nucleic acid, the target nucleic acid, and a denaturant chemical, which mixture is incubated at elevated temperature for a short time, are disclosed. Compositions that include a capture probe that specifically binds to a target nucleic acid and a denaturant chemical, which when mixed with the target nucleic acid and incubated at elevated temperature for a short time, promote efficient hybridization of the capture probe and target nucleic acid are disclosed.

    Abstract translation: 通过使用含有目标核酸特异性的捕获探针，目标核酸和变性剂化合物的混合物在高温下短时间培养来有效地从样品中捕获靶核酸的方法是： 披露。 公开了包含与目标核酸和变性剂化学物质特异性结合的捕获探针的组合物，当与目标核酸混合并在高温下温育短时间时，促进了捕获探针和靶核酸的有效杂交 。

    CAPTURE PROBES IMMOBILIZABLE VIA L-NUCLEOTIDE TAIL
    8.
    发明申请
    CAPTURE PROBES IMMOBILIZABLE VIA L-NUCLEOTIDE TAIL 审中-公开
    捕获探针通过L-核核酸尾部可维持

    公开(公告)号：WO2012037531A1

    公开(公告)日：2012-03-22

    申请号：PCT/US2011/052050

    申请日：2011-09-16

    Applicant: Gen-Probe Incorporated ,  POLLNER, Reinhold ,  MAJLESSI, Mehrdad ,  YAMAGATA, Susan ,  BECKER, Michael, M. ,  REYNOLDS, Mark ,  ARNOLD, Lyle

    Inventor： POLLNER, Reinhold ,  MAJLESSI, Mehrdad ,  YAMAGATA, Susan ,  BECKER, Michael, M. ,  REYNOLDS, Mark ,  ARNOLD, Lyle

    IPC: C12Q1/68

    CPC classification number: C12Q1/707 ,  C12Q1/6837 ,  C12Q1/703 ,  Y02A50/53 ,  Y02A50/54 ,  C12Q2525/161

    Abstract: The invention provides chimeric capture probes immobilizable via an L-nucleic acid tail that can bind to a complementary L-nucleic acid in an immobilized probe. The capture probes are useful for capturing a target nucleic acid from a sample. The L-nucleic acid in the tail of the capture probe bind to the complementary L-nucleic acid in the immobilized probe with similar affinity as would otherwise equivalent D-nucleic acids. However, the L-nucleic acid of the capture probe tail and immobilized probes do not form stable duplexes with D-nucleic acids present in the in the sample containing the target nucleic acid. Binding of nucleic acids in the sample directly to immobilized probe or to the tail of the capture probe is reduced or eliminated increasing the sensitivity and/or specificity of the assay.

    Abstract translation: 本发明提供了通过可以在固定化探针中结合互补L-核酸的L-核酸尾部固定的嵌合捕获探针。 捕获探针可用于从样品中捕获靶核酸。 捕获探针尾部的L-核酸与固定化探针中的互补L-核酸结合，具有与否则相当于D-核酸的亲和力。 然而，捕获探针尾和固定化探针的L-核酸不与存在于含有靶核酸的样品中的D-核酸形成稳定的双链体。 将样品中的核酸直接与捕获探针的固定化探针或尾部的结合降低或消除，增加了测定的灵敏度和/或特异性。

    METHODS AND KITS FOR AMPLIFYING DNA
    9.
    发明申请
    METHODS AND KITS FOR AMPLIFYING DNA 审中-公开
    用于扩增DNA的方法和工具

    公开(公告)号：WO2008108843A3

    公开(公告)日：2008-10-30

    申请号：PCT/US2007063103

    申请日：2007-03-01

    Applicant: GEN PROBE INC ,  BECKER MICHAEL M ,  LAM WAI-CHUNG ,  LIVEZEY KRISTIN W ,  BRENTANO STEVEN T ,  KOLK DANIEL P ,  SCHRODER ASTRID R W

    Inventor： BECKER MICHAEL M ,  LAM WAI-CHUNG ,  LIVEZEY KRISTIN W ,  BRENTANO STEVEN T ,  KOLK DANIEL P ,  SCHRODER ASTRID R W

    IPC: C12Q1/68 ,  C07H21/02

    CPC classification number: C12Q1/6865 ,  C12Q2533/101 ,  C12Q2525/186 ,  C12Q2521/107

    Abstract: Novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic are disclosed (/. e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, methods of nucleic acid amplification are disclosed which are robust and efficient, while reducing the appearance of side-products. In general, the methods use priming oligonucleotides that target only one sense of a target nucleic acid, a promoter oligonucleotide modified to prevent polymerase extension from its 3 '-terminus and, optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or substantially eliminating the formation of side-products. The disclosed methods minimizes or substantially eliminate the emergence of side-products, thus providing ahigh level of specificity. Furthermore, the appearance of side-products can complicate the analysis of the amplification reaction by various molecular detection techniques. The disclosed methods minimize or substantially eliminate this problem, thus providing enhanced levels of sensitivity.

    Abstract translation: 公开了合成自动催化的靶核酸序列的多个拷贝的新方法（例如，能够自动循环而不需要修饰反应条件如温度，pH或离子强度，并且使用一个循环的产物 在下一个）。 特别地，公开了鲁棒和有效的核酸扩增方法，同时减少副产物的外观。 通常，所述方法使用仅靶向靶核酸的引物寡核苷酸，修饰以阻止聚合酶从其3'末端延伸的启动子寡核苷酸，以及任选的终止引物延伸反应的手段以扩增RNA或 DNA分子在体外，同时减少或基本上消除副产物的形成。 所公开的方法最小化或基本上消除副产物的出现，从而提供高水平的特异性。 此外，副产物的出现可能通过各种分子检测技术使扩增反应的分析复杂化。 所公开的方法最小化或基本上消除了该问题，从而提供了增强的灵敏度水平。

    METHODS OF NONSPECIFIC TARGET CAPTURE OF NUCLEIC ACIDS
    10.
    发明申请
    METHODS OF NONSPECIFIC TARGET CAPTURE OF NUCLEIC ACIDS 审中-公开
    核酸非靶向捕获方法

    公开(公告)号：WO2008016988A1

    公开(公告)日：2008-02-07

    申请号：PCT/US2007074990

    申请日：2007-08-01

    Applicant: GEN PROBE INC ,  BECKER MICHAEL M ,  MAJLESSI MEHRDAD R

    Inventor： BECKER MICHAEL M ,  MAJLESSI MEHRDAD R

    IPC: C12Q1/68 ,  C07H21/04

    CPC classification number: C12N15/1006 ,  C12N15/11 ,  C12N2310/18 ,  C12N2310/31 ,  C12N2310/321 ,  C12N2310/351 ,  C12N2330/30 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/70 ,  C12Q2565/519 ,  C12Q2565/518

    Abstract: Methods for capturing a target nucleic acid from a sample by using a capture probe that binds nonspecifically to the target nucleic acid and binds specifically to an immobilized probe via a specific binding pair that has one member on the capture probe and one member on the immobilized probe are disclosed. Compositions that include a capture probe that binds nonspecifically to a target nucleic acid and specifically to an immobilized probe via binding of members of a specific binding pair in a solution phase of a reaction mixture are disclosed.

    Abstract translation: 通过使用捕获探针非特异性结合靶核酸并通过在捕获探针上具有一个成员的特异性结合对与固定化探针上的一个成员特异性结合固定的探针来从样品捕获靶核酸的方法 被披露。 公开了包含捕获探针的组合物，其通过结合反应混合物的溶液相中的特异性结合对的成员非特异性地结合靶核酸而特异性结合固定的探针。

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