公开(公告)号：US20060051036A1

公开(公告)日：2006-03-09

申请号：US10956044

申请日：2004-10-04

Applicant: Patrick Treado ,  Matthew Nelson ,  Joseph Demuth

Inventor： Patrick Treado ,  Matthew Nelson ,  Joseph Demuth

IPC: G02B6/06 ,  G01J3/44

CPC classification number: A61B1/00165 ,  A61B1/043 ,  A61B1/07 ,  A61B5/0075 ,  A61B5/0084 ,  G01J3/02 ,  G01J3/021 ,  G01J3/0218 ,  G01J3/0256 ,  G01J3/0264 ,  G01J3/0291 ,  G01J3/32 ,  G01J3/44 ,  G01J2003/1213 ,  G01N21/33 ,  G01N21/359 ,  G01N21/474 ,  G01N21/645 ,  G01N21/65 ,  G01N2021/3155 ,  G01N2021/4742 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6484 ,  G01N2021/656 ,  G02B6/06 ,  G02B6/29398 ,  G02B23/2423 ,  G02B23/2469

Abstract: The disclosure generally relates to a method and apparatus for a fiberscope. In one embodiment, the disclosure relates to a chemical imaging fiberscope for imaging and collecting optical spectra from a sample having at least one illumination fiber for transmitting light from a first an a second light source to a distal end of a fiberscope; a dichroic mirror disposed at said distal end of the fiberscope such that light from said first light source passes substantially straight through said mirror and light of a predetermined wavelength from said second light source is substantially reflected by said mirror toward said sample to thereby illuminate said sample; and at least one collection fiber for receiving light from said illuminated sample and transmitting the received light to an optical device.

公开(公告)号：US20060001867A1

公开(公告)日：2006-01-05

申请号：US10879632

申请日：2004-06-30

Applicant: John Maier ,  Jason Neiss ,  Shona Stewart ,  Matthew Nelson ,  Joseph Demuth ,  Patrick Treado

Inventor： John Maier ,  Jason Neiss ,  Shona Stewart ,  Matthew Nelson ,  Joseph Demuth ,  Patrick Treado

IPC: G01J3/44 ,  G01N21/65

CPC classification number: G01N21/65 ,  G01J3/0297 ,  G01J3/44 ,  G01N2021/656

Abstract: The embodiments disclosed herein generally relate to identifying and removing background noise in spectroscopic imaging of a sample. Because white-light has essentially constant intensity at every wavelength, background noise caused by white light can be identified and removed from spectroscopic measurements including Raman spectroscopy. Thus, once the Raman spectrum for a sample is obtained, it may be corrected to remove the white-light dispersive spectrum in accordance with the embodiments disclosed herein.

Abstract translation: 本文公开的实施例通常涉及在样本的光谱成像中识别和去除背景噪声。 因为白光在每个波长处具有基本恒定的强度，所以可以从包括拉曼光谱的光谱测量中识别和去除由白光引起的背景噪声。 因此，一旦获得样品的拉曼光谱，就可以根据本文公开的实施方案来校正其去除白光色散谱。

公开(公告)号：US20070178067A1

公开(公告)日：2007-08-02

申请号：US11598248

申请日：2006-11-09

Applicant: John Maier ,  Joseph Demuth ,  Jeffrey Cohen ,  Lindy McClelland ,  Shona Stewart

Inventor： John Maier ,  Joseph Demuth ,  Jeffrey Cohen ,  Lindy McClelland ,  Shona Stewart

IPC: A61K48/00 ,  C12Q1/00 ,  G06F19/00

CPC classification number: G01N33/5005 ,  G01N15/1459 ,  G01N21/65 ,  G01N33/574 ,  G01N33/57407 ,  G01N2021/656

Abstract: A method and system of differentially manipulating cells where the cells, suspended in a fluid, are irradiated with substantially monochromatic light. A Raman data set is obtained from the irradiated cells and where the data set is characteristic of a disease status. The data set is assessed to identify diseased cells. A Raman chemical image of the irradiated cells is also obtained. Based on the assessment and the Raman chemical image, the fluid in which the cells are suspended is differentially manipulated. The diseased cells are directed to a first location and other non-diseased cells are directed to a second location as part of the differential manipulation. The diseased cells may be treated with a physical stress, a chemical stress, and a biological stress and then returned to a patient from whom the diseased cells were obtained prior to the irradiation.

Abstract translation: 一种差异操纵细胞的方法和系统，其中悬浮在流体中的细胞用基本上单色的光照射。 从照射的细胞获得拉曼数据集，其中数据集是疾病状态的特征。 评估数据集以鉴定患病细胞。 还获得了照射的细胞的拉曼化学图像。 基于评估和拉曼化学图像，细胞悬浮的流体被差异地操纵。 病变细胞被引导到第一位置，并且其他非病变细胞被引导到第二位置作为差异操纵的一部分。 患病细胞可以用物理应力，化学应力和生物应激治疗，然后返回到在照射之前从其获得患病细胞的患者。

公开(公告)号：US07239383B2

公开(公告)日：2007-07-03

申请号：US10879632

申请日：2004-06-30

Applicant: John S. Maier ,  Jason N. Neiss ,  Shona Stewart ,  Matthew P. Nelson ,  Joseph Demuth ,  Patrick J. Treado

Inventor： John S. Maier ,  Jason N. Neiss ,  Shona Stewart ,  Matthew P. Nelson ,  Joseph Demuth ,  Patrick J. Treado

IPC: G01J3/44

CPC classification number: G01N21/65 ,  G01J3/0297 ,  G01J3/44 ,  G01N2021/656

Abstract: The embodiments disclosed herein generally relate to identifying and removing background noise in spectroscopic imaging of a sample. Because white-light has essentially constant intensity at every wavelength, background noise caused by white light can be identified and removed from spectroscopic measurements including Raman spectroscopy. Thus, once the Raman spectrum for a sample is obtained, it may be corrected to remove the white-light dispersive spectrum in accordance with the embodiments disclosed herein.

Abstract translation: 本文公开的实施例通常涉及在样本的光谱成像中识别和去除背景噪声。 因为白光在每个波长处具有基本恒定的强度，所以可以从包括拉曼光谱的光谱测量中识别和去除由白光引起的背景噪声。 因此，一旦获得样品的拉曼光谱，就可以根据本文公开的实施方案来校正其去除白光色散谱。

公开(公告)号：US20050277816A1

公开(公告)日：2005-12-15

申请号：US11000591

申请日：2004-11-30

Applicant: John Maier ,  Joseph Demuth ,  Jeffrey Cohen ,  Shona Stewart ,  Lindy McClelland

Inventor： John Maier ,  Joseph Demuth ,  Jeffrey Cohen ,  Shona Stewart ,  Lindy McClelland

IPC: A61B5/00

CPC classification number: A61B5/0084 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/415 ,  A61B5/417 ,  A61B5/418 ,  A61B5/4519 ,  G01N21/65

Abstract: Raman molecular imaging is used to differentiate between normal and diseased cells or tissue. For instance benign and malignant lesions of bladder and other tissues can be distinguished, including epithelial tissues such as lung, prostate, kidney, breast, and colon, and non-epithelial tissues, such as bone marrow and brain. Raman scattering data relevant to the disease state of cells or tissue can be combined with visual image data to produce hybrid images which depict both a magnified view of the cellular structures and information relating to the disease state of the individual cells in the field of view.

Abstract translation: 拉曼分子成像用于区分正常和病变细胞或组织。 例如，可以区分膀胱和其他组织的良性和恶性病变，包括上皮组织如肺，前列腺，肾脏，乳腺和结肠，以及非上皮组织，如骨髓和脑。 可以将与细胞或组织的疾病状态相关的拉曼散射数据与视觉图像数据组合，以产生描绘细胞结构的放大视图和与视野中的各个细胞的疾病状态有关的信息的混合图像。

公开(公告)号：US08553732B2

公开(公告)日：2013-10-08

申请号：US12755463

申请日：2010-06-23

Applicant: John Maier ,  Joseph Demuth ,  Jeffrey Cohen ,  Shona Stewart ,  Lindy McClelland

Inventor： John Maier ,  Joseph Demuth ,  Jeffrey Cohen ,  Shona Stewart ,  Lindy McClelland

IPC: H01S3/00

CPC classification number: A61B5/0084 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/415 ,  A61B5/417 ,  A61B5/418 ,  A61B5/4519 ,  G01N21/65

Abstract: A method for generating an image of a sample that is informative of the disease state of a cell in the sample. A sample including the cell is irradiated with monochromatic light. The Raman scattered light is assessed. A digital brightfield image of the Raman scattered light is generated and combined with the Raman scattered light emitted by the cell whereby the Raman scattered light is informative of the disease state of the cell in the sample. The method can also be used to determine the metabolic activity of the cell, the inflammatory status of the cell and/or the infected status of the cell in the sample.

Abstract translation: 一种产生样品图像的方法，其用于提供样品中细胞的疾病状态。 用单色光照射包含电池的样品。 评估拉曼散射光。 产生拉曼散射光的数字明场图像，并与细胞发射的拉曼散射光组合，由此拉曼散射光提供样品中细胞的疾病状态。 该方法还可以用于确定细胞的代谢活性，细胞的炎性状态和/或样品中细胞的感染状态。

公开(公告)号：US20060001868A1

公开(公告)日：2006-01-05

申请号：US10879633

申请日：2004-06-30

Applicant: Shona Stewart ,  Matthew Nelson ,  Joseph Demuth ,  Patrick Treado

Inventor： Shona Stewart ,  Matthew Nelson ,  Joseph Demuth ,  Patrick Treado

IPC: G01J3/44 ,  G01N21/65

CPC classification number: G01J3/44 ,  G01J3/0297 ,  G01N21/65

Abstract: The disclosure relates to a substrate material for the improved detection, resolution and imaging of biological material for spectroscopic characterization by Raman of optical imaging spectroscopy. The substrate provides a uniform, optically flat, highly reflective surface which can be made hydrophobic to prevent spreading of the sample and facilitating its optical evaluation. Moreover, the substrate can be coated with a material that does not emit Raman scattered photons when exposed to said illuminating photons. The principles disclosed herein allow a low spectroscopic background particularly suitable for examining small samples or samples having low concentrations of the suspected component.

Abstract translation: 本公开涉及用于通过光学成像光谱法的拉曼进行光谱表征的生物材料的改进的检测，分辨和成像的基底材料。 衬底提供均匀的光学平坦的高反射表面，其可以被制成疏水性以防止样品的扩散并且便于其光学评估。 此外，当暴露于所述照射光子时，衬底可以涂覆有不发射拉曼散射光子的材料。 本文公开的原理允许低光谱背景特别适合于检查具有低浓度可疑成分的小样品或样品。