公开(公告)号：CA2771597C

公开(公告)日：2018-01-09

申请号：CA2771597

申请日：2010-08-19

Applicant: NATIONAL UNIV CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： KATO KO ,  KANAYA SHIGEHIKO ,  MATSUURA HIDEYUKI ,  TAKENAMI SHINYA ,  NOSHO TATSUYA ,  KUBO YUHKI ,  UEDA KIYOTAKA

IPC: C12N15/09 ,  A01H1/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10

Abstract: A sequence characteristic of a 5'UTR that is involved in the change in the state of translation in a plant under environmental stresses is found. Provided are: a recombinant DNA molecule having the sequence characteristic; an expression vector; a transformant; and others. Specifically provided are: a recombinant DNA molecule encoding mRNA having a 5'UTR (a) or (b) as mentioned below; a vector comprising the recombinant DNA molecule ligated just behind the transcription initiation point of a promoter; and a transformant carrying the vector: (a) a 5'UTR in which a nucleotide sequence lying between the 1st nucleotide and the 7th nucleotide as numbered from the 5'-terminal thereof and a nucleotide sequence lying between the 12th nucleotide and the 32nd nucleotide as numbered from the 5'-terminal thereof are a nucleotide sequence lying between the 1st nucleotide and the 7th nucleotide as numbered from the 5'-terminal of a nucleotide sequence represented by SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:20, SEQ ID NO:36 or SEQ ID NO:60 and a nucleotide sequence lying between the 12th nucleotide and the 32nd nucleotide as numbered from the 5'-terminal of the nucleotide sequence represented by SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:20, SEQ ID NO:36 or SEQ ID NO:60, respectively; and (b) a 5'UTR which is produced by substituting one or several nucleotides in the nucleotide sequence for the 5'UTR (a), and which can prevent the inhibition of translation under at least one environmental stress selected from the group consisting of a thermal stress and a salt stress.

公开(公告)号：CA2984402A1

公开(公告)日：2016-11-03

申请号：CA2984402

申请日：2016-04-21

Applicant: NATIONAL UNIV CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： KATO KO

IPC: C12N15/113 ,  C12N5/14 ,  C12N15/09 ,  C12P21/00

Abstract: The present invention addresses the problem of developing a DNA molecule encoding a 5'UTR that enables the high-level expression of a recombinant protein in a plant, and providing a technique for producing a recombinant protein efficiently. A nucleic acid construct composed of a polynucleotide encoding a protein and a 5'UTR comprising a nucleotide sequence represented by SEQ ID NO: 1 or 2 or a variant of the 5'UTR, wherein the 5'UTR is linked to the polynucleotide. By using the nucleic acid construct, it becomes possible to efficiently produce a recombinant protein in a plant.

公开(公告)号：DE102024120661A8

公开(公告)日：2025-03-13

申请号：DE102024120661

申请日：2024-07-19

Applicant: NATIONAL UNIV CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY ,  SUZUKI MOTOR CORP

Inventor： KIDA SHOGO ,  SEO HIROYUKI ,  HORITA HIDENORI ,  RAKUMATSU TAKESHI ,  ORITA YASUAKI

IPC: A61B5/18 ,  A61B5/103 ,  B60R16/037 ,  G08B21/00 ,  H04N23/611

公开(公告)号：CA2984402C

公开(公告)日：2022-08-16

申请号：CA2984402

申请日：2016-04-21

Applicant: NATIONAL UNIV CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： KATO KO

IPC: C12N15/113 ,  C12N5/14 ,  C12N15/09 ,  C12P21/00

Abstract: The present invention addresses the problem of developing a DNA molecule encoding a 5'UTR that enables the high-level expression of a recombinant protein in a plant, and providing a technique for producing a recombinant protein efficiently. A nucleic acid construct composed of a polynucleotide encoding a protein and a 5'UTR comprising a nucleotide sequence represented by SEQ ID NO: 1 or 2 or a variant of the 5'UTR, wherein the 5'UTR is linked to the polynucleotide. By using the nucleic acid construct, it becomes possible to efficiently produce a recombinant protein in a plant.

公开(公告)号：AU2019434270A1

公开(公告)日：2021-09-30

申请号：AU2019434270

申请日：2019-03-14

Applicant: MEDICAGO INC ,  MITSUBISHI TANABE PHARMA CORP ,  NATIONAL UNIV CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： LAVOIE PIERRE-OLIVIER ,  D'AOUST MARC-ANDRE ,  KATO KO ,  YAMASAKI SHOTARO

IPC: C12N15/113 ,  A01H5/00 ,  C07K14/08 ,  C07K14/11 ,  C12N5/10 ,  C12N15/13 ,  C12N15/40 ,  C12N15/44 ,  C12N15/82 ,  C12P21/02

Abstract: An isolated expression enhancer active in a plant, portion of a plant or plant cell, the expression enhancer is provided. The isolated expression enhancer may be selected from the group consisting of nbEPI42 (SEQ ID NO:1); nbSNS46 (SEQ ID NO:2); nbCSY65 (SEQ ID NO:3); nbHEL40 (SEQ ID NO:4); and nbSEP44 (SEQ ID NO:5). Methods for using the isolated expression enhancer are also provided.

公开(公告)号：CA3133166A1

公开(公告)日：2020-09-17

申请号：CA3133166

申请日：2019-03-14

Applicant: MITSUBISHI TANABE PHARMA CORP ,  MEDICAGO INC ,  NATIONAL UNIV CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： KATO KO ,  YAMASAKI SHOTARO ,  D'AOUST MARC-ANDRE ,  LAVOIE PIERRE-OLIVIER

IPC: C12N15/113 ,  A01H5/00 ,  C07K14/08 ,  C07K14/11 ,  C12N5/10 ,  C12N15/13 ,  C12N15/40 ,  C12N15/44 ,  C12N15/82 ,  C12P21/02

Abstract: An isolated expression enhancer active in a plant, portion of a plant or plant cell, the expression enhancer is provided. The isolated expression enhancer may be selected from the group consisting of nbEPI42 (SEQ ID NO:1); nbSNS46 (SEQ ID NO:2); nbCSY65 (SEQ ID NO:3); nbHEL40 (SEQ ID NO:4); and nbSEP44 (SEQ ID NO:5). Methods for using the isolated expression enhancer are also provided.

公开(公告)号：CA2857622C

公开(公告)日：2019-08-27

申请号：CA2857622

申请日：2012-11-20

Applicant: NATIONAL UNIV CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： TAKAGI HIROSHI ,  SASANO YU ,  SHIMA JUN ,  HAITANI YUTAKA

IPC: C12N1/19 ,  A21D2/08 ,  A21D8/04 ,  C07K14/39 ,  C12N15/09 ,  C12Q1/02

Abstract: The present invention provides yeast having increased resistance to freezing stress in which POG1 gene is inactivated, a method for producing such yeast, and a method for using such yeast in food production.

公开(公告)号：DK2728004T3

公开(公告)日：2018-11-05

申请号：DK14152631

申请日：2009-04-28

Applicant: IDEMITSU KOSAN CO ,  NATIONAL UNIV CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： SAWADA KAZUTOSHI ,  YOSHIDA KAZUYA ,  MATSUI TAKESHI

IPC: A01H5/00 ,  A61K39/00 ,  A61K39/106 ,  A61K39/108 ,  A61K39/112 ,  A61P31/04 ,  C07K14/245 ,  C07K14/25 ,  C07K14/28 ,  C07K19/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/82

公开(公告)号：ES2686780T3

公开(公告)日：2018-10-19

申请号：ES09738819

申请日：2009-04-28

Applicant: IDEMITSU KOSAN CO ,  NATIONAL UNIV CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： SAWADA KAZUTOSHI ,  YOSHIDA KAZUYA ,  MATSUI TAKESHI

IPC: C12N15/09 ,  A01H5/00 ,  A61K39/106 ,  A61K39/108 ,  A61K39/112 ,  A61P31/04 ,  C07K14/245 ,  C07K14/25 ,  C07K14/28 ,  C07K19/00 ,  C12N5/10

Abstract: Una proteína híbrida que comprende (a) dos proteínas toxinas seleccionadas del grupo que consiste en proteína toxina Shiga (Stx), proteína toxina del cólera (CT) o proteína toxina lábil al calor de Escherichia coli (LT), en donde dichas proteínas toxinas se unen en tándem a través de un péptido que tiene la secuencia de aminoácidos representada por SEQ ID NO: 2, 82 o 84, y (b) un péptido que tiene la secuencia de aminoácidos representada por SEQ ID NO: 2, 82 o 84 unido al extremo C de las proteínas toxinas unidas en tándem de (a), en donde la proteína Stx es una proteína Stx2eB, en donde la proteína CT es una subunidad B de la proteína CT, y en donde la proteína LT es una subunidad B de la proteína LT.

公开(公告)号：WO2006109197A2

公开(公告)日：2006-10-19

申请号：PCT/IB2006/001741

申请日：2006-02-28

Applicant: NARA INSTITUTE OF SCIENCE AND TECHNOLOGY ,  HASHIMOTO, Takashi ,  KATO, Akira

Inventor： HASHIMOTO, Takashi ,  KATO, Akira

CPC classification number: A24B15/18 ,  A23V2002/00 ,  A24B13/00 ,  A24B13/02 ,  A24B15/20 ,  A24B15/243 ,  A24D1/00 ,  C07K14/415 ,  C12N15/8218 ,  C12N15/8243 ,  C12Q2600/158

Abstract: Two genes, A622 and NBBl, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBBl may be used to decrease nicotine in tobacco plants.

Abstract translation: 可以影响两个基因A622和NBB1，以实现植物中烟碱型生物碱水平的降低。 特别地，抑制A622和NBB1中的一种或两种可以用于减少烟草植物中的尼古丁。