公开(公告)号：WO2007109126A2

公开(公告)日：2007-09-27

申请号：PCT/US2007/006624

申请日：2007-03-16

Applicant: DUKE UNIVERSITY ,  PALMER, Gregory, M. ,  RAMANUJAM, Nirmala

Inventor： PALMER, Gregory, M. ,  RAMANUJAM, Nirmala

IPC: A61B6/00 ,  G01J3/30

CPC classification number: G01J3/4406 ,  A61B5/0071 ,  A61B5/0084 ,  G01N21/49 ,  G01N21/6486 ,  G01N2021/6417

Abstract: The subject matter described herein includes a method for modeling fluorescence in turbid media and methods and systems for using the model to determine intrinsic fluorescence of turbid media. According to one aspect, a method for modeling fluorescence of a turbid medium and for using the model to determine intrinsic fluorescence in the turbid medium is provided. The method includes illuminating a turbid medium of interest with an electromagnetic radiation source using a probe of a particular geometry and detecting measured fluorescence for the turbid medium using the probe. At least one set of Monte Carlo simulations is run to determine an escape energy probability map and an absorbed energy density map for the turbid medium. An indication of the intrinsic fluorescence of the turbid medium is determined using the escape probability density map and the absorbed energy density map in a manner that accounts for the geometry of the probe.

Abstract translation: 本文描述的主题包括用于对混浊介质中的荧光进行建模的方法以及使用该模型确定混浊介质的固有荧光的方法和系统。 根据一个方面，提供了一种用于对浑浊介质的荧光进行建模和使用该模型来确定混浊介质中的固有荧光的方法。 该方法包括使用特定几何形状的探针用电磁辐射源照射感兴趣的混浊介质，并使用探针检测浊度介质的测量荧光。 运行至少一组蒙特卡罗模拟来确定浑浊介质的逃逸能量概率图和吸收的能量密度图。 使用逃避概率密度图和吸收的能量密度图以考虑探针的几何形状的方式确定混浊介质的固有荧光的指示。

公开(公告)号：WO2011068998A3

公开(公告)日：2011-11-17

申请号：PCT/US2010058771

申请日：2010-12-02

Applicant: UNIV DUKE ,  RAMANUJAM NIRMALA ,  PHELPS JANELLE E

Inventor： RAMANUJAM NIRMALA ,  PHELPS JANELLE E

IPC: G01N33/49 ,  A61B5/1455 ,  G01N21/47 ,  G01N33/72

CPC classification number: G01N21/49 ,  A61B5/0059 ,  A61B5/0077 ,  G01N21/314 ,  G01N21/4738 ,  G01N2201/129

Abstract: Systems and methods for determining hemoglobin concentration utilizing diffuse reflectance at isosbestic wavelengths are provided. In one embodiment, the method includes emitting light onto a tissue mass, measuring diffuse reflectance from the tissue mass, and calculating a ratio of diffuse reflectance intensities at two isosbestic wavelengths using the measured diffuse reflectance. The method also includes determining a hemoglobin concentration associated with the tissue mass by applying the calculated ratio to a predefined analytical expression associated with the two isosbestic wavelengths.

Abstract translation: 提供了利用等离子体波长的漫反射测定血红蛋白浓度的系统和方法。 在一个实施例中，该方法包括将光发射到组织块上，测量来自组织块的漫反射率，以及使用所测量的漫反射率计算两个等腰波长处的漫反射强度的比率。 该方法还包括通过将所计算的比率应用于与两个等腰波长相关联的预定解析表达式来确定与组织块相关的血红蛋白浓度。

公开(公告)号：WO2010042249A3

公开(公告)日：2010-06-10

申请号：PCT/US2009041732

申请日：2009-04-24

Applicant: UNIV DUKE ,  YU BING ,  RAMANUJAM NIRMALA ,  LO JUSTIN Y

Inventor： YU BING ,  RAMANUJAM NIRMALA ,  LO JUSTIN Y

IPC: A61B5/00 ,  A61B5/1455

CPC classification number: A61B5/0059

Abstract: A diffuse reflectance spectroscopy system for quantifying electromagnetic absorption and scattering in a tissue is provided. Also provided are optical probes and methods for imaging a tissue mass. In some embodiments, the methods include the steps of contacting a tissue mass with an optical probe, wherein the optical probe includes at least one entity for emitting light that interacts with a tissue mass and then is remitted to a collecting entity, for collecting the light that has interacted with the tissue mass, wherein the collecting entity comprises a detector comprising one or more photodiodes; measuring turbid spectral data of the tissue mass using the optical probe; converting the turbid spectral data to at least one of absorption and scattering spectral data via a Monte Carlo algorithm or a diffusion algorithm; and quantifying tissue compositions and scatterer size in a tissue mass using the at least one of absorption and scattering spectral data.

公开(公告)号：WO2009043045A1

公开(公告)日：2009-04-02

申请号：PCT/US2008/078186

申请日：2008-09-29

Applicant: DUKE UNIVERSITY ,  RAMANUJAM, Nirmala ,  YU, Bing ,  BROWN, J., Quincy ,  SHANG, Allan, B.

Inventor： RAMANUJAM, Nirmala ,  YU, Bing ,  BROWN, J., Quincy ,  SHANG, Allan, B.

IPC: A61B5/00

CPC classification number: A61B5/0091 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/0086 ,  A61B5/413 ,  A61B5/4312

Abstract: Systems and methods for spectral analysis of a tissue mass using an instrument, an optical probe, and a Monte Carlo algorithm or a diffusion algorithm are provided. According to one method, an instrument is inserted into a tissue mass. A fiber optic probe is applied via the instrument into the tissue mass. Turbid spectral data of the tissue mass is measured using the fiber probe. The turbid spectral data is converted to absorption, scattering, and/or intrinsic fluorescence spectral data via a Monte Carlo algorithm or diffusion algorithm. Biomarker concentrations in the tissue mass are quantified using the absorption, scattering, and/or intrinsic fluorescence spectral data.

Abstract translation: 提供了使用仪器，光学探针和蒙特卡洛算法或扩散算法对组织块进行光谱分析的系统和方法。 根据一种方法，将器械插入组织块中。 通过仪器将光纤探头施加到组织块中。 使用光纤探针测量组织块的浊度光谱数据。 通过蒙特卡罗算法或扩散算法将浊度光谱数据转换为吸收，散射和/或固有荧光光谱数据。 使用吸收，散射和/或固有的荧光光谱数据来量化组织块中的生物标志物浓度。

公开(公告)号：WO2008103486A1

公开(公告)日：2008-08-28

申请号：PCT/US2008/002431

申请日：2008-02-25

Applicant: DUKE UNIVERSITY ,  WISCONSIN ALUMNI RESEARCH FOUNDATION ,  RAMANUJAM, Nirmala ,  LIU, Quan

Inventor： RAMANUJAM, Nirmala ,  LIU, Quan

IPC: A61B6/00

CPC classification number: A61B5/0059 ,  G01N21/49 ,  G06F17/5009 ,  G06F2217/10 ,  G06F2217/16

Abstract: The presently disclosed subject matter relates to multilayered scaling methods that allow for implementation of fast Monte Carlo simulations of diffuse reflectance from multilayered turbid media. The disclosed methods employ photon trajectory information provided by only a single baseline simulation, from which the diffuse reflectance can be computed for a wide range of optical properties in a multilayered turbid medium. A convolution scheme is also incorporated to calculate diffuse reflectance for specific fiber optic probe geometries. Also provided are systems for fast Monte Carlo simulation of diffuse reflectance of a multilayered turbid medium to rapidly determine diffuse reflectance for the multilayered turbid medium with known optical properties and for using the scaled diffuse reflectance to determine optical properties of a turbid medium having unknown optical properties.

Abstract translation: 目前公开的主题涉及允许从多层混浊介质实施漫反射的快速蒙特卡罗模拟的多层缩放方法。 所公开的方法使用仅由单个基线模拟提供的光子轨迹信息，可以从多层基础模拟中计算多层混浊介质中宽范围的光学性质的漫反射。 还结合卷积方案来计算特定光纤探针几何形状的漫反射。 还提供了用于快速蒙特卡罗模拟多层混浊介质的漫反射的系统，以快速确定具有已知光学性质的多层混浊介质的漫反射，并且使用缩放漫反射度来确定具有未知光学特性的混浊介质的光学特性 。

公开(公告)号：WO2015077684A1

公开(公告)日：2015-05-28

申请号：PCT/US2014/067038

申请日：2014-11-24

Applicant: DUKE UNIVERSITY ,  RAMANUJAM, Nirmala ,  HU, Fangyao ,  LAM, Christopher

Inventor： RAMANUJAM, Nirmala ,  HU, Fangyao ,  LAM, Christopher

IPC: A61B1/303 ,  A61B1/06

CPC classification number: A61B1/0676 ,  A61B1/00045 ,  A61B1/00082 ,  A61B1/00087 ,  A61B1/00089 ,  A61B1/00091 ,  A61B1/012 ,  A61B1/05 ,  A61B1/0638 ,  A61B1/0646 ,  A61B1/0684 ,  A61B1/303

Abstract: Colposcopes having light emitters and image capture devices and associated methods are disclosed. According to an aspect, a colposcope includes an elongate body having a distal end, a proximate end, and an axis extending between the distal end and the proximate end. The colposcope also includes a balloon attached to the elongate body and configured to be inflated to expand in a direction away from the axis of the elongate body. Further the colposcope includes an image capture device attached to the distal end of the elongate body and positioned to capture images of an area outside the elongate body. The colposcope also includes one or more light emitters attached to the distal end of the elongate body and positioned to generate and direct light towards the area outside of the elongate body.

Abstract translation: 公开了具有发光体和图像捕获装置及相关方法的阴道镜。 根据一个方面，阴道镜包括细长体，其具有远端，近端和在远端和近端之间延伸的轴线。 阴道镜还包括附接到细长主体并被配置为膨胀以在远离细长主体的轴线的方向上膨胀的气囊。 此外，阴道镜包括附接到细长主体的远端并定位成捕获细长体外部的区域的图像的图像捕获装置。 阴道镜还包括附接到细长主体的远端的一个或多个光发射器，并被定位成产生并引导朝向细长主体外部区域的光。

公开(公告)号：WO2009043050A3

公开(公告)日：2009-05-28

申请号：PCT/US2008078194

申请日：2008-09-29

Applicant: UNIV DUKE ,  RAMANUJAM NIRMALA ,  YU BING ,  BROWN QUINCY J ,  SHANG ALLAN B

Inventor： RAMANUJAM NIRMALA ,  YU BING ,  BROWN QUINCY J ,  SHANG ALLAN B

IPC: A61B8/00 ,  G01N21/27

CPC classification number: G01N21/4795 ,  A61B5/0059 ,  G01N21/6456 ,  G01N21/6486 ,  G01N2021/4747 ,  G01N2021/6484

Abstract: The subject matter described herein relates to an optical assay system having a multi-probe imaging array that orients a plurality of probes with respect to one another and to a sample. According to one aspect, the subject matter described herein includes a multi-probe imaging array for contacting biological samples and simultaneously illuminating a plurality of locations on the biological sample and collecting the reflected radiation from the locations. The multi-probe imaging array can be used for the rapid imaging of biological samples, for example, during surgery.

Abstract translation: 本文所述的主题涉及具有多探针成像阵列的光学测定系统，所述多探针成像阵列使多个探针相对于彼此和样品定向。 根据一个方面，本文所述的主题包括用于接触生物样品并且同时照射生物样品上的多个位置并从该位置收集反射的辐射的多探针成像阵列。 多探针成像阵列可用于生物样品的快速成像，例如在手术过程中。

公开(公告)号：WO2007126827A3

公开(公告)日：2008-12-18

申请号：PCT/US2007007586

申请日：2007-03-29

Applicant: UNIV DUKE ,  RAMANUJAM NIRMALA ,  WILKE LEE G

Inventor： RAMANUJAM NIRMALA ,  WILKE LEE G

IPC: A61N5/02 ,  A61B5/05 ,  A61B6/00 ,  A61B8/00

CPC classification number: G01N21/4738

Abstract: The subject matter described herein includes an optical assay system for intraoperative assessment of tumor margins. According to one aspect, the subject matter described herein includes a biological sample containment and illumination apparatus for holding a biological sample for illumination by a plurality of electromagnetic radiation probes. The biological sample containment and illumination apparatus includes a plurality of frame members positioned with respect to each other to form an interior space for receiving a biological sample. At least one of the plurality of frame members includes a plurality of probe receiving locations for receiving a plurality of electromagnetic radiation probes. The probe receiving locations position the probes with respect to the biological sample to allow illumination of plural locations of the biological sample by the probes.

Abstract translation: 本文所述的主题包括用于术中评估肿瘤边缘的光学测定系统。 根据一个方面，本文所述的主题包括用于通过多个电磁辐射探针保持用于照明的生物样品的生物样品容纳物和照明装置。 生物样品容纳和照明装置包括相对于彼此定位的多个框架构件，以形成用于接收生物样品的内部空间。 多个框架构件中的至少一个包括用于接收多个电磁辐射探针的多个探针接收位置。 探针接收位置使探针相对于生物样品定位，以允许通过探针照射生物样品的多个位置。

公开(公告)号：WO2007100648A3

公开(公告)日：2007-09-07

申请号：PCT/US2007/004705

申请日：2007-02-26

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION ,  LIU, Quan ,  RAMANUJAM, Nirmala

Inventor： LIU, Quan ,  RAMANUJAM, Nirmala

IPC: A61B5/103 ,  A61B5/00 ,  G01N21/17

Abstract: A reflectance instrument illuminates the surface of tissue with light of a selected wavelength and light emanating from the tissue due to reflectance is collected. The angle of illumination of tissue surface and/or collection of reflections is changed to probe at various depths beneath the surface of the tissue. The reflectance instrument may be used in a method for measuring the optical properties of a two layer diffuse media such as epithelial tissues.

公开(公告)号：WO2007100648A2

公开(公告)日：2007-09-07

申请号：PCT/US2007004705

申请日：2007-02-26

Applicant: WISCONSIN ALUMNI RES FOUND ,  LIU QUAN ,  RAMANUJAM NIRMALA

Inventor： LIU QUAN ,  RAMANUJAM NIRMALA

IPC: A61B5/103 ,  A61B5/00

CPC classification number: A61B5/0059 ,  A61B2562/0242 ,  G01N21/4738 ,  G01N21/4795 ,  G01N21/49 ,  G01N2021/1782 ,  G01N2021/4742

Abstract: A reflectance instrument illuminates the surface of tissue with light of a selected wavelength and light emanating from the tissue due to reflectance is collected. The angle of illumination of tissue surface and/or collection of reflections is changed to probe at various depths beneath the surface of the tissue. The reflectance instrument may be used in a method for measuring the optical properties of a two layer diffuse media such as epithelial tissues.

Abstract translation: 反射仪器用选定波长的光照射组织表面，并收集由于反射而从组织发出的光。 改变组织表面的照射角度和/或反射的集合以探测组织表面下的各种深度。 反射仪器可以用于测量诸如上皮组织的双层扩散介质的光学性质的方法。