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    Oil bodies and associated proteins as affinity matrices
    13.
    发明申请
    Oil bodies and associated proteins as affinity matrices 审中-公开
    油体和相关蛋白质作为亲和基质

    公开(公告)号：US20030096320A1

    公开(公告)日：2003-05-22

    申请号：US10260562

    申请日：2002-10-01

    Applicant: SemBioSys Genetics Inc.

    Inventor： Maurice Moloney ,  Joseph Boothe ,  Gijs Van Rooijen

    IPC: G01N033/53 ,  C07K014/415

    CPC classification number: C07H21/00 ,  B01D15/08 ,  B01D15/3804 ,  B01D15/3809 ,  C01G11/003 ,  C07K1/22 ,  C07K14/415 ,  C07K16/065 ,  C08B1/00 ,  C12N9/6429 ,  C12Y304/21005

    Abstract: A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions.

    Abstract translation: 描述了从混合物中分离靶分子的方法。 该方法使用油体及其相关蛋白作为所需靶分子的选择性，非共价结合的亲和基质。 油体蛋白质可以遗传融合到对所需靶分子具有特异性的配体。 天然油体蛋白质也可以与油体蛋白质特异性配体如抗体或油体结合蛋白结合使用。 该方法允许由于油体和水溶液之间的密度差而使所需目标分子分离和回收。

    POLYUNSATURATED FATTY ACID PRODUCTION IN HETEROLOGOUS ORGANISMS USING PUFA POLYKETIDE SYNTHASE SYSTEMS
    16.
    发明申请
    POLYUNSATURATED FATTY ACID PRODUCTION IN HETEROLOGOUS ORGANISMS USING PUFA POLYKETIDE SYNTHASE SYSTEMS 审中-公开
    使用多不饱和脂多糖合成酶系统进行异构体生物聚合脂肪酸生产

    公开(公告)号：WO2007106905A3

    公开(公告)日：2008-11-27

    申请号：PCT/US2007064106

    申请日：2007-03-15

    Applicant: MARTEK BIOSCIENCES CORP ,  SEMBIOSYS GENETICS INC ,  METZ JAMES G ,  KUNER JERRY M ,  LIPPMEIER JAMES CASEY ,  MOLONEY MAURICE MARTIN ,  NYKIFORUK CORY LEE

    Inventor： METZ JAMES G ,  KUNER JERRY M ,  LIPPMEIER JAMES CASEY ,  MOLONEY MAURICE MARTIN ,  NYKIFORUK CORY LEE

    IPC: A01H5/00 ,  C12N15/82

    CPC classification number: C12N9/1029 ,  C12N9/93 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472 ,  Y02P20/52

    Abstract: Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the plant or plant cell, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.

    Abstract translation: 公开了新的酰基辅酶A合成酶和新的酰基转移酶，编码它们的核酸分子，重组核酸分子和包含该核酸分子的重组宿主细胞，包含该核酸分子的转基因生物（微生物和植物），以及制备和使用 一样。 还公开了经遗传修饰以表达用于生产PUFA（PUFA PKS系统或PUFA合酶）的PKS样系统的遗传修饰生物体（例如，植物，微生物），其中生物体被修饰以表达酰基 -CoA合成酶，以表达酰基转移酶，以删除或失活由生物表达的脂肪酸合成酶（FAS），以减少与PUFA合酶的丙二酰辅酶A竞争或增加植物或植物细胞中丙二酰辅酶A的水平 ，并且在一个方面，抑制KASII或KASIII。 除了PUFA和从这些生物获得的油之外，还公开了另外的修饰和制造和使用这些生物体的方法，其中包括各种产品，包括这种PUFA和油。

    METHODS FOR THE PRODUCTION OF APOLIPOPROTEINS IN TRANSGENIC PLANTS
    17.
    发明申请
    METHODS FOR THE PRODUCTION OF APOLIPOPROTEINS IN TRANSGENIC PLANTS 审中-公开
    在转基因植物中生产亚磷酸蛋白酶的方法

    公开(公告)号：WO2005047455A2

    公开(公告)日：2005-05-26

    申请号：PCT/CA2004/001960

    申请日：2004-11-15

    Applicant: SEMBIOSYS GENETICS INC. ,  MOLONEY, Maurice, M. ,  REID, Alexandra

    Inventor： MOLONEY, Maurice, M. ,  REID, Alexandra

    IPC: C12N

    CPC classification number: C07K14/775 ,  C12N15/8257

    Abstract: Methods for the production of an apolipoprotein in plants are described. In one embodiment, the present invention provides a method for the expression of apolipoprotein in plants comprising : (a) providing a chimeric nucleic acid construct comprising in the 5’ to 3’ direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant cells; and (ii) a nucleic acid sequence encoding an apolipoprotein polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and growing the plant cell into a mature plant capable of setting seed wherein the seed expresses apolipoprotein.

    Abstract translation: 描述了在植物中产生载脂蛋白的方法。 在一个实施方案中，本发明提供了一种用于在植物中表达载脂蛋白的方法，包括：（a）提供嵌合核酸构建体，其以5'至3'的转录方向包含可操作地连接的组分：（i）核酸 能够控制植物细胞中表达的序列; 和（ii）编码载脂蛋白多肽的核酸序列; （b）将嵌合核酸构建体引入植物细胞; 并将植物细胞生长成能够设定种子的成熟植物，其中种子表达载脂蛋白。

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