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公开(公告)号：KR101391204B1
公开(公告)日：2014-05-07
申请号：KR1020130099743
申请日：2013-08-22
Applicant: (주)다림티센 , 울산대학교 산학협력단
Abstract: 본발명은고순도의아텔로콜라겐을이온화시키는과정을통하여양이온화아텔로콜라겐을제조한후 이를이용하여췌도세포를배양하는방법및 췌도세포이식용담체를제조하는방법그리고이를이용하여제조된인공췌장을제공한다.본발명에따르면고순도의아텔로콜라겐을이온화시키는과정을통하여수득된양이온화아텔로콜라겐또는가교화된아텔로콜라겐지지체를이용하여췌도세포의배양시췌도세포의생존율및/또는글루코오스의존적인인슐린분비량을높일수 있고, 양이온화아텔로콜라겐과알지네이트를포함하는안정성이우수한췌도세포이식용담체를제공함으로써배양및 이식된췌도세포의생존율을높이면서동시에글루코오스의존적인인슐린분비량을높일수 있는장점이있다.
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公开(公告)号：KR1020110058757A
公开(公告)日：2011-06-01
申请号：KR1020110036663
申请日：2011-04-20
Applicant: (주)다림티센
Inventor： 박시내
IPC: A61K38/39 , A61K31/728 , A61P41/00 , C12P21/06
CPC classification number: A61K38/39 , A61K9/70 , A61K31/728 , C12P21/06
Abstract: PURPOSE: A method for manufacturing an anti-adhesion agent using esterified collagen and hyaluronic acid is provided to help wound healing and tissue reconstruction with safety. CONSTITUTION: A method for manufacturing an anti-adhesion agent using esterified collagen and hyaluronic acid comprises: a step of treating pepsin to natural collagen to prepare attelo collagen; a step of adding 0.5-3 wt% of attelo collagen to 65-85 wt% of ethanol solution and stirring; a step of adding 0.5M acetic acid or hydrochloric acid and reacting at pH7.4 and 4°C for a week to obtain an esterified collagen solution; a step of freeze-drying the collagen solution to obtain esterified collagen; a step of adding 0.5-3 wt% of esterified collagen to purified solution and stirring at 4°C for 24 hours; and a step of preparing esterified collagen porous film; and a step of dipping the porous film in 0.5-5 wt% of purified solution and reacting at 4°C for 24 hours to prepare a porous film composite of esterified collagen and hyaluronic acid.
Abstract translation: 目的：提供使用酯化胶原和透明质酸制造抗粘连剂的方法，以安全地帮助伤口愈合和组织重建。 构成：使用酯化胶原和透明质酸制造抗粘连剂的方法包括：将胃蛋白酶处理成天然胶原以制备胶原蛋白的步骤; 将0.5-3wt％的attelo胶原蛋白加入到65-85wt％乙醇溶液中并搅拌的步骤; 加入0.5M乙酸或盐酸并在pH7.4和4℃下反应一周以获得酯化胶原溶液的步骤; 冷冻干燥胶原溶液以获得酯化胶原的步骤; 将0.5-3重量％的酯化胶原蛋白加入到纯化溶液中并在4℃下搅拌24小时的步骤; 和制备酯化胶原多孔膜的步骤; 以及在0.5-5重量％的纯化溶液中浸渍多孔膜并在4℃下反应24小时以制备酯化胶原和透明质酸的多孔膜复合物的步骤。
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公开(公告)号：KR1020100111383A
公开(公告)日：2010-10-15
申请号：KR1020090029784
申请日：2009-04-07
Applicant: (주)다림티센
Inventor： 박시내
IPC: A61K38/39 , A61K31/728 , A61P41/00 , C12P21/06
CPC classification number: A61K38/39 , A61K9/70 , A61K31/728 , C12P21/06
Abstract: PURPOSE: A method for manufacturing an anti-adhesion agent of porous film, film, gel, and microsome using esterification collagen is provided to ensure safety and biocompatibility. CONSTITUTION: A method for manufacturing an anti-adhesion agent using esterification collagen and hyaluronic acid comprises: a step of treating the natural collagen with pepsin to obtain atelocolagen; a step of adding 0.5-3 wt% of atelocollagen to 65-85 wt% of ethanol solution and stirring; a step of adjusting by pH 7.4 using 0.5M of acetic acid or hydrochloric acid; a step of reacting at 4°C for a week to obtain esterification collagen; a step of freeze-drying the esterification collagen; and a step of preparing a complex of esterification collagen and hyaluronic acid.
Abstract translation: 目的：提供使用酯化胶原制造多孔膜，膜，凝胶和微粒体的抗粘附剂的方法，以确保安全性和生物相容性。 构成：使用酯化胶原和透明质酸制造抗粘连剂的方法包括：用胃蛋白酶处理天然胶原蛋白以获得无胶原蛋白的步骤; 在65-85重量％的乙醇溶液中加入0.5-3重量％的胶原蛋白并搅拌的步骤; 使用0.5M乙酸或盐酸调节pH 7.4的步骤; 在4℃下反应一周以获得酯化胶原的步骤; 冷冻干燥酯化胶原的步骤; 以及制备酯化胶原和透明质酸的复合物的步骤。
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公开(公告)号：KR101158338B1
公开(公告)日：2012-06-22
申请号：KR1020100045322
申请日：2010-05-14
Applicant: (주)다림티센
CPC classification number: C07K14/78 , A61L27/24 , Y10T428/249988
Abstract: 본 발명의 아텔로콜라겐 분리방법은, (a) 텔로펩타이드가 제거된 아텔로 콜라겐을 포함하는 시료를 용기 내에 준비하는 단계와, (b) 상기 아텔로 콜라겐을 포함하는 시료를 상기 용기로부터 여과 멤브레인이 구비된 여과모듈로 유입시키고, 상기 여과모듈에 압력을 인가하여 상기 시료가 상기 여과 멤브레인을 통과하면서 여과되도록 하는 한외여과 과정을 수행하는 단계와, (c) 상기 한외여과 과정을 통해 상기 여과 멤브레인을 통과하면서 여과되어 상기 여과모듈로부터 유출되는 아텔로 콜라겐 용액을 수집하는 단계와, (d) 상기 여과 멤브레인을 통과하면서 여과되는 아텔로 콜라겐 용액의 유량을 측정하여 한외여과 속도를 결정하는 단계와, (e) 상기 한외여과 속도가 일정한 속도 이하로 감소되면 상기 한외여과 과정을 중지하는 단계와, (f) 상기 시료 중 상기 여과 멤브레인을 통과하지 못하고 상기 용기로 복귀되는 잔류물에 물을 첨가한 후 이를 상기 여과 멤브레인이 구비된 여과모듈로 유입시켜 정용여과 과정을 수행하는 단계와, (g) 상기 정용여과 과정을 통해 상기 여과 멤브레인을 통과하면서 여과되어 상기 여과모듈로부터 유출되는 아텔로 콜라겐 용액을 수집하는 단계와, (h) 상기 (f) 단계와 상기 (g) 단계를 반복하는 단계를 포함한다.
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公开(公告)号：KR1020100028608A
公开(公告)日：2010-03-12
申请号：KR1020100007230
申请日：2010-01-27
Applicant: (주)다림티센
Abstract: PURPOSE: A collagen base matrix as a texture restoration material and a method for manufacturing the same are provided, which can enhance the mechanical strength and prevent the porous film from being partly destroyed in the manufacturing process. CONSTITUTION: A method for manufacturing a collagen base matrix as a texture restoration material comprises: a step of forming a collagen porous layer by freeze-drying after forming the uniform film of the constant thickness by dispersing the collagen in the fixed thickness; and a step of forming a collagen hard layer with a dense tissue by compacting collagen particles and discharging water.
Abstract translation: 目的：提供作为纹理恢复材料的胶原基质基质及其制造方法，其可以在制造过程中增强机械强度并防止多孔膜部分破坏。 构成：作为纹理恢复材料的胶原基质基质的制造方法包括：通过将固定厚度的胶原分散而形成固定厚度的均匀膜后，通过冷冻干燥形成胶原多孔层的工序; 以及通过压实胶原颗粒并排出水来形成具有致密组织的胶原蛋白硬质层的步骤。
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Patent Agency Ranking
公开(公告)号：KR101735560B1
公开(公告)日：2017-05-16
申请号：KR1020150045477
申请日：2015-03-31
Applicant: 재단법인 아산사회복지재단 , (주)다림티센
Abstract: 본발명은양이온화아텔로콜라겐, 헤파린, 췌도세포및 중간엽줄기세포의복합체를포함하는당뇨병치료용약학조성물, 상기복합체를포함하는췌도세포이식용조성물및 상기복합체를포함하는인공췌장에관한것이다. 본발명에서제공하는양이온화아텔로콜라겐, 헤파린, 췌도세포및 중간엽줄기세포의복합체를포함하는조성물은, 그자체로서인슐린을효과적으로분비생산할수 있을뿐만아니라췌도세포를안정화시킬수 있으므로, 보다효과적인당뇨병의치료에널리활용될수 있을것이다.
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公开(公告)号：KR1020130101488A
公开(公告)日：2013-09-13
申请号：KR1020130099743
申请日：2013-08-22
Applicant: (주)다림티센 , 울산대학교 산학협력단
CPC classification number: A61L27/24 , A61K35/39 , A61L27/3804 , C12N5/0676 , C12N11/02 , C12N2533/54 , C12N2533/74
Abstract: PURPOSE: A method for culturing islet cells using atelocollagen is provided to effectively culture islet cells using a cationized atelocollagen or crosslinked atelocollagen support which is acquired by ionizing high purity atelocollagen. CONSTITUTION: A method for culturing islet cells using atelocollagen comprises the steps of preparing a cationized atelocollagen solution; applying the cationized atelocollagen solution on a culture container and drying to form a cationized atelocollagen support; inoculating islet cells to the cationized atelocollagen solution; and culturing the inoculated islet cells in the cationized atelocollagen solution or on the cationized atelocollagen support. [Reference numerals] (AA) Number of islet cells; (BB) 1 day; (CC) 3 weeks; (DD) 8 weeks
Abstract translation: 目的：提供使用小胶原蛋白培养胰岛细胞的方法，以有效培养胰岛细胞，使用通过离子化高纯度胶原蛋白获得的阳离子化的阿胶原或交联的阿胶原载体。 构成：使用阿胶囊培养胰岛细胞的方法包括制备阳离子化的胶原蛋白溶液的步骤; 将阳离子化的胶原凝胶溶液施加到培养容器上并干燥以形成阳离子化的胶原蛋白载体; 将胰岛细胞接种到阳离子化的胶原蛋白溶液中; 并在阳离子化的胶原蛋白溶液或阳离子化的胶原蛋白载体上培养接种的胰岛细胞。 （附图标记）（AA）胰岛细胞数; （BB）1天; （CC）3周; （DD）8周
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公开(公告)号：KR1020120001704A
公开(公告)日：2012-01-04
申请号：KR1020110120742
申请日：2011-11-18
Applicant: (주)다림티센
Abstract: PURPOSE: A method for preparing esterified atellocollagen is provided to ensure excellent biocompatibility and to be applied in various types. CONSTITUTION: A method for preparing esterified atellocollagen comprises: a step of preparing atellocollagen dispersion solution and adding acid; a step of neutralizing atellocollagen dispersion solution; a step of centrifuging the atellocollagen dispersion solution to collect esterified atellocollagen precipitate; a step of performing dialysis of the precipitate in purified water; and a step of freeze-drying the precipitate.
Abstract translation: 目的：提供一种制备酯化的atolacollagen的方法，以确保优异的生物相容性并应用于各种类型。 构成：制备酯化的atolacollagen的方法包括：制备atellocollagen分散液并加入酸的步骤; 中和阿托洛胶分散液的步骤; 离心该胶原分散液以收集酯化的胶原沉淀物的步骤; 在纯净水中进行沉淀物透析的步骤; 和冷冻干燥沉淀物的步骤。
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公开(公告)号：KR1020110125772A
公开(公告)日：2011-11-22
申请号：KR1020100045322
申请日：2010-05-14
Applicant: (주)다림티센
CPC classification number: C07K14/78 , A61L27/24 , Y10T428/249988
Abstract: PURPOSE: A method for isolating atelocollagen is provided to efficiently and simply remove impurity through a single process. CONSTITUTION: A method for isolating atelocollagen comprises: a step of preparing a sample containing telopeptide-removed atelocollagen in a container; a step of passing the sample containing atelocollagen through a filter; a step of collecting atelocollagen solution and measuring flow of the atelocollagen solution to determine ultrafiltration speed; a step of stopping ultrafiltration; a step of adding water to unfiltered materials and performing diafiltration process; and a step of collecting filtered atelocollagen solution.
Abstract translation: 目的：提供一种隔离无胶原蛋白的方法，以通过单一过程有效和简单地去除杂质。 构成：分离无胶原蛋白的方法包括：在容器中制备含有去离子肽胶原的样品的步骤; 使含有胶原蛋白的样品通过过滤器的步骤; 收集胶原蛋白溶液并测量阿胶囊溶液的流量以确定超滤速度的步骤; 停止超滤的一个步骤; 向未过滤材料加水并进行渗滤的步骤; 和收集过滤的胶原蛋白溶液的步骤。
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