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公开(公告)号：KR101280462B1
公开(公告)日：2013-07-01
申请号：KR1020100113663
申请日：2010-11-16
Applicant: (주)셀트리온
Abstract: 본 발명은 CHO 세포 DNA 검출 또는 정량용 조성물에 관한 것으로, 보다 구체적으로 CHO 세포 DNA를 특이적으로 검출할 수 있는 프라이머 및 프로브에 관한 것이다. 본 발명의 프라이머 및 이를 함유하는 조성물은 CHO 세포 DNA만을 특이적으로 검출할 수 있어, 상기 세포를 숙주세포로 사용하여 바이오 의약품을 제조하는 과정에서 생산되는 상기 세포의 배양물이나 정제물에 함유된 CHO 세포 DNA를 효과적으로 검출하거나 정량하는데 용이하게 사용될 수 있다.
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公开(公告)号：KR1020210042052A
公开(公告)日：2021-04-16
申请号：KR1020210029750
申请日：2021-03-05
Applicant: (주)셀트리온
Abstract: 본발명에따른안정한액체약제학적제제는항체또는이의항원결합단편; 계면활성제; 당또는이의유도체; 및완충제를포함한다. 본발명에따른안정한액체약제학적제제는항체를고함량으로포함하면서낮은점도를가지고, 가속조건및 가혹조건에서의우수한안정성을바탕으로장기간보관안정성이우수하고, 피하투여가가능하다.
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Patent Agency Ranking
公开(公告)号：KR1020130060246A
公开(公告)日：2013-06-07
申请号：KR1020130055409
申请日：2013-05-16
Applicant: (주)셀트리온
CPC classification number: C12N15/11 , C12Q1/6851 , C12Q1/6876 , C12Q2563/107
Abstract: PURPOSE: A composition for detecting or quantitating Sp2/0 cell DNA is provided to specifically detect only Sp2/0 cell DNA and to effectively detect and quantitate Sp2/0 cell DNA contained in a cell culture or purified material thereof. CONSTITUTION: A primer set for detecting Sp2/0 cell DNA contains base sequences of sequence numbers 1 and 2. A composition for detecting or quantitating Sp2/0 cell DNA contains the primer set and a probe of sequence number 3. A method for detecting or quantitating Sp2/0 cell DNA comprises: a step of isolating DNA from a sample; a step of performing PCR using the primer set and the probe; and a step of measuring fluorescent signal.
Abstract translation: 目的：提供用于检测或定量Sp2 / 0细胞DNA的组合物，以特异性检测Sp2 / 0细胞DNA，并有效检测和定量细胞培养物中所含的Sp2 / 0细胞DNA或其纯化物质。 构成：用于检测Sp2 / 0细胞DNA的引物组含有序列号1和2的碱基序列。用于检测或定量Sp2 / 0细胞DNA的组合物含有引物组和序列号3的探针。一种检测或 定量的Sp2 / 0细胞DNA包括：从样品中分离DNA的步骤; 使用引物组和探针进行PCR的步骤; 以及测量荧光信号的步骤。
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公开(公告)号：KR101302398B1
公开(公告)日：2013-09-02
申请号：KR1020110013931
申请日：2011-02-17
Applicant: (주)셀트리온
Abstract: 본 발명은 Sp2/0 세포 DNA 검출 또는 정량용 조성물에 관한 것으로, 보다 구체적으로 Sp2/0 세포 DNA를 특이적으로 검출할 수 있는 프라이머 및 프로브에 관한 것이다. 본 발명의 프라이머 및 이를 함유하는 조성물은 Sp2/0 세포 DNA만을 특이적으로 검출할 수 있어, 상기 세포를 숙주세포로 사용하여 바이오 의약품을 제조하는 과정에서 생산되는 상기 세포의 배양물이나 정제물에 함유된 Sp2/0 세포 DNA를 효과적으로 검출하거나 정량하는데 용이하게 사용될 수 있다.
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公开(公告)号：KR1020130041860A
公开(公告)日：2013-04-25
申请号：KR1020130030055
申请日：2013-03-21
Applicant: (주)셀트리온
CPC classification number: C12N15/11 , C12Q1/686 , C12Q1/6876 , C12Q2561/113
Abstract: PURPOSE: A composition for detection or quantitation of CHO cell DNA is provided to effectively detect or quantitate DNA contained in a CHO cell culture or a purified material thereof. CONSTITUTION: A composition for detection or quantitation of CHO(Chinese hamster ovary) cell DNA contains a primer set of sequence numbers 1 and 2 and a probe of sequence number 3. A fluorescent marker is inserted into one end of the probe and a fluorescent repressor is inserted into the other end. A method for detection or quantitation of CHO cell DNA comprises: a step of isolating DNA from a sample; a step of performing PCR of the DNA using the primer set and the probe; and a step of measuring a fluorescent signal.
Abstract translation: 目的：提供用于CHO细胞DNA检测或定量的组合物，以有效地检测或定量CHO细胞培养物中所含的DNA或其纯化材料。 构成：用于检测或定量CHO（中国仓鼠卵巢）细胞DNA的组合物含有序列号1和2的引物组和序列号3的探针。将荧光标记插入探针的一端和荧光阻遏物 插入另一端。 用于CHO细胞DNA的检测或定量的方法包括：从样品中分离DNA的步骤; 使用引物组和探针进行DNA的PCR的步骤; 以及测量荧光信号的步骤。
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公开(公告)号：KR1020120094581A
公开(公告)日：2012-08-27
申请号：KR1020110013931
申请日：2011-02-17
Applicant: (주)셀트리온
CPC classification number: C12Q1/6888 , C12Q1/6851
Abstract: PURPOSE: A composition for detection or quantification of Sp2/0 cell DNA and a primer set for the same are provided to specifically detect the Sp2/0 cell DNA only, thereby being used for effectively detecting or quantifying the Sp2/0 cell DNA contained in cultures or purified materials of the cell. CONSTITUTION: Primer sets for detection or quantification of Sp2/0 cell DNA are marked by the sequence number 1(SEQ ID NO:1) and the base sequence of 2. The composition for detection or quantification of the Sp2/0 cell DNA comprises the primer sets marked by base sequences having the sequence number 1 and 2 and a probe marked by the base sequence having the sequence number of 3. A fluorescent label factor is inserted into one end of the probe, and a fluorescence inhibitory substance is inserted into the opposite end of the probe. The Sp2/0 cell DNA is the genomic DNA of the Sp2/0 cell remaining in the sample. A kit for detection or quantification of the Sp2/0 cell DNA comprises the composition for detection and quantification of the Sp2/0 cell DNA. A detection or quantification method of the Sp2/0 cell comprises the following steps: extracting DNA from the sample; performing PCR(polymerase chain reaction) the extracted DNA using the primer set marked by base sequences having the sequence number 1 and 2 and a probe marked by the base sequence having the sequence number 3; and measuring fluorescence signals.
Abstract translation: 目的：提供用于检测或定量Sp2 / 0细胞DNA的组合物及其引物组，用于仅特异性检测Sp2 / 0细胞DNA，从而用于有效检测或定量含有的Sp2 / 0细胞DNA 细胞的培养物或纯化物质。 构成：用于Sp2 / 0细胞DNA的检测或定量的引物组由序列号1（SEQ ID NO：1）和碱基序列2标记。用于检测或定量的Sp2 / 0细胞DNA的组合物包括 由序列号为1和2的碱基序列标记的引物组和由序列号为3的碱基序列标记的探针。将荧光标记因子插入探针的一端，并将荧光抑制物质插入 探针的相对端。 Sp2 / 0细胞DNA是残留在样品中的Sp2 / 0细胞的基因组DNA。 用于检测或定量Sp2 / 0细胞DNA的试剂盒包含用于检测和定量Sp2 / 0细胞DNA的组合物。 Sp2 / 0细胞的检测或定量方法包括以下步骤：从样品中提取DNA; 使用由序列号1和2的碱基序列标记的引物组和由序列号3的碱基序列标记的探针进行PCR（聚合酶链式反应）提取的DNA; 并测量荧光信号。
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公开(公告)号：KR1020120052484A
公开(公告)日：2012-05-24
申请号：KR1020100113663
申请日：2010-11-16
Applicant: (주)셀트리온
CPC classification number: C12Q1/6876 , C12Q1/6851
Abstract: PURPOSE: A composition for detecting or quantitating DNA from CHO cells is provided to specifically detect DNA and to manufacture bio medicinal products using host cells. CONSTITUTION: A primer set for detecting DNA from CHO(Chinese hamster ovary) cell is denoted by sequence numbers 1 and 2. A composition for detecting or quantitating DNA of CHO cells contains the primer set of sequence numbers 1 and 2 and a probe of sequence number 3. The DNA of CHO cells is genomic DNA. A method for detecting or quantitating DNA of CHO cells comprises: a step of isolating DNA from a sample; a step of performing PCR using the primer and probe; and a step of measuring fluorescence signal.
Abstract translation: 目的：提供用于检测或定量来自CHO细胞的DNA的组合物，以特异性检测DNA并使用宿主细胞制造生物医药产品。 构成：用于检测来自CHO（中国仓鼠卵巢）细胞的DNA的引物组由序列号1和2表示。用于检测或定量CHO细胞的DNA的组合物含有序列号1和2的引物组和序列的探针 CHO细胞的DNA是基因组DNA。 用于检测或定量CHO细胞DNA的方法包括：从样品中分离DNA的步骤; 使用引物和探针进行PCR的步骤; 以及测量荧光信号的步骤。
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