Abstract:
본 발명은 사육수 순환 여과 장치를 이용한 육상 수조식 해삼 양식 장치 및 이를 이용한 육상 수조식 해삼 양식 방법을 제공한다. 본 발명의 해삼 양식 장치는 해삼의 생리·생태적인 특성을 고려한, 수조의 내부에 격막을 설치하여 해삼이 오탁물이 침전된 저면에서 공간적으로 분리되도록 한 양식 수조를 포함한다. 또한 사육수는 생물 여과 방식을 이용하여 여과하여 재급수하는 순환 여과 방식이 사용된다. 또한 본 발명의 해삼 양식 장치는 구비된 수온 조절 장치를 사용하여 사육수의 수온 조절이 가능하다. 상기 해삼 양식 장치는 사육수의 배수 문제와 해삼의 성장을 모두 고려한 특수한 구조의 양식 수조, 사육수 순환 여과 장치 및 수온 조절 장치를 결합함으로써 해삼의 하면 문제를 해결하였으며, 노지 양식과 비해 해삼의 대량 생산이 가능하다.
Abstract:
A use of fish feed containing carotenoid, highly unsaturated fatty acid and conjugated linoleic acid as an active ingredient is provided to ensure improvement in the growth of fish and oxidation stability of fish meat while converting conjugated linoleic acid to lipid components of fish when fed to fish. Fish feed containing fish meal, soybean meal, wheat flour, cuttlefish liver oil, vitamin, mineral and soybean oil is characterized in that it contains 0.5% by weight of carotenoid selected from natural carotenoid and synthetic carotenoid, 5 to 25% by weight of highly unsaturated fatty acid and 1 to 5% by weight of conjugated linoleic acid, based on the total weight of the fish feed. The fish feed overcomes the problems of inhibiting the growth of fish when a quantity of conjugated linoleic acid is fed to fish and improves meat quality by accumulation of carotenoid pigments when fed to fish.
Abstract:
A method for preparing a glycosaminoglycan from ascidian tunic is provided to obtain the glycosaminoglycan with high yield and high purity more economically by removing a low molecular weight protein and a pigment compound using an ion exchange resin. A method for preparing a highly pure glycosaminoglycan comprises the steps of: (a) crushing ascidian tunic preserved at a temperature of -40 deg.C and steaming the crushed tunic at a temperature of 105-121.1 deg.C for 1-2 hours; (b) after adding 1.0-5.0 wt.% of flavourzyme 500 MG to the steamed tunic, treating it with a buffer solution with the pH of 5.0-8.0 at a temperature of 40-65 deg.C for 4-6 hours to decompose enzymatically; (c) after centrifuging the enzymatically decomposed tunic with the speed of 6,000-8,000 rpm, concentrating a filtered supernatant using a vacuum evaporator at a temperature of 30-60 deg.C into Brix of 8.0-15.0; (d) after centrifuging the concentrate solution and adjusting the pH of an obtained supernatant into 5.5-7.5, putting it in a cation exchange resin column and eluting it to purify; and (e) after adding alcohol to a filtered solution obtained from the step(d) to precipitate and centrifuging it, vacuum freeze-drying the precipitate.