公开(公告)号：KR1020120065103A

公开(公告)日：2012-06-20

申请号：KR1020100126440

申请日：2010-12-10

Applicant: 고려대학교 산학협력단

Inventor： 이영식 ,  홍기창 ,  김준모 ,  임도환 ,  홍재상 ,  임규상 ,  이동현

IPC: C12N15/12 ,  C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/6876 ,  C12Q2600/124

Abstract: PURPOSE: A DNA marker containing SNP of PPARGC1A gene 3'UTR region is provided to improve meat quality and to select individuals with high quality. CONSTITUTION: A DNA marker for detecting pork meat quality contains 15-800 continuous sequences with 2956th base, C, in sequence number 1. A primer for detecting SNP relating meat quality increase in the 3'UTR region contains an oligonucleotide of sequence numbers 2 and 3. A method for detecting increase of meat quality in a pig comprises: a step of isolating genomic DNA from the pig; a step of amplifying a specific site containing 2956th base of 3'UTR; a step of analyzing a base sequence of amplifies DNA; and a step of identifying substitution of 2956th base from T to C.

Abstract translation: 目的：提供含有PPARGC1A基因3'UTR区域的SNP的DNA标记，以提高肉质，并选择高质量的个体。 构成：用于检测猪肉质量的DNA标记物包含15-800个连续序列，第2956位碱基序列号1。用于检测与3'UTR区域的肉质量增加相关的SNP的引物含有序列号2和 3.一种用于检测猪肉质量增加的方法，包括：从猪分离基因组DNA的步骤; 扩增含有2956碱基3'UTR的特异性位点的步骤; 分析扩增DNA的碱基序列的步骤; 并且识别从T到C的第2956位碱基的取代的步骤。

公开(公告)号：KR1020120065039A

公开(公告)日：2012-06-20

申请号：KR1020100126347

申请日：2010-12-10

Applicant: 고려대학교 산학협력단

Inventor： 이영식 ,  홍기창 ,  김준모 ,  임도환 ,  홍재상 ,  임규상 ,  이동현

IPC: C12N15/12 ,  C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/683 ,  C12Q2600/124

Abstract: PURPOSE: A DNA marker containing SNP of primary microRNA is provided to enhance muscle fiber type I in pork muscle fiber and to increase pH concentration. CONSTITUTION: A DNA marker for detecting increase of pork meat quality contains 15-600 serial sequences including 495th base, C, in sequence number 1. A primer for detecting meat quality increase-associated SNP in a pork microRNA-133b precursor contains an oligonucleotide of sequences 3 and 4. A kit for SNP analysis contains HphI which is a restriction enzyme for analyzing RFLP, the primer, and PCR reaction mixture.

Abstract translation: 目的：提供含有初级微小RNA的SNP的DNA标记，以增强猪肉肌纤维中的肌纤维I型，并增加pH浓度。 构成：用于检测猪肉质量增加的DNA标记物包含15-600个连续序列，包括序列号1中的第495位碱基C。用于检测猪肉微RNA-133b前体中肉质增加相关SNP的引物含有寡核苷酸 序列3和4.用于SNP分析的试剂盒含有HphI，其是用于分析RFLP，引物和PCR反应混合物的限制酶。