-
公开(公告)号：KR1020030005642A
公开(公告)日：2003-01-23
申请号：KR1020010041011
申请日：2001-07-09
Applicant: 광주과학기술원
IPC: C12N15/81
Abstract: PURPOSE: Provided is a method for measuring the substrate specificity of the protease without involving additional separation and purification of the protease and synthesis of a substrate peptide, thereby simply and cheaply carrying out the gene sequencing of the protease. CONSTITUTION: A plasmid pADH-kex2 contains a kex2 gene downstream to an ADH promoter. A plasmid pADH-SteSubInv contains cDNA which encodes a portion containing a golgi apparatus target signal region and a transmembrane region of a golgi apparatus protein of yeast, Ste13, a recognition site of alpha-factor of yeast being known as a substrate of kex2, and a fusion protein containing an invertase of yeast, and which is positioned downstream of the ADH promoter. The method for measuring the substrate specificity of the protease comprises the steps of: (i) constructing a vector containing a gene encoding a protease; (ii) constructing a vector containing a gene encoding a golgi apparatus target signal region and a transmembrane region of a membrane protein in a golgi apparatus of yeast, a gene encoding a substrate region having a specific amino acid sequence, and a gene encoding an invertase of yeast; (iii) producing a transformant by significantly transforming yeast mutant suc2 with the two vectors; and (iv) culturing the transformant in a medium containing sucrose as a sole carbon source and measuring survival rate of the transformant.
Abstract translation: 目的：提供了一种用于测量蛋白酶的底物特异性而不涉及蛋白酶的另外分离和纯化以及底物肽的合成的方法，从而简单且便宜地进行蛋白酶的基因测序。 构成：质粒pADH-kex2含有ADH启动子下游的kex2基因。 质粒pADH-SteSubInv含有cDNA，其编码含有高尔基体装置靶信号区和酵母的高尔基体装置蛋白的跨膜区的部分，Ste13是酵母的α因子的识别位点，被称为kex2的底物，以及 含有酵母转化酶并位于ADH启动子下游的融合蛋白。 用于测量蛋白酶底物特异性的方法包括以下步骤：（i）构建含有编码蛋白酶的基因的载体; （ii）构建含有编码具有特定氨基酸序列的底物区域的基因的编码高尔基体装置靶信号区域和膜蛋白的跨膜区域的基因的载体，以及编码转化酶 的酵母 （iii）通过用两个载体显着转化酵母突变suc2产生转化体; 和（iv）在含有蔗糖作为唯一碳源的培养基中培养转化体并测定转化体的存活率。
-
公开(公告)号：KR1020140010889A
公开(公告)日：2014-01-27
申请号：KR1020130079156
申请日：2013-07-05
Applicant: 광주과학기술원
CPC classification number: C07K7/06 , A61K38/005 , A61K38/08 , C07K7/00 , C07K14/00 , C07K14/47 , C07K14/4703
Abstract: The present invention relates to a decoy peptide or polypeptide consisting of a peptide sequence represented by General Formula I below: X_1-Ala-X_2-X_3-Ile-Glu-X_4 (I) wherein X_1 is 0-50 amino acid residues; X_2 is Ser, Glu, or Asp; X_3 is Thr, Glu, or Asp; X_4 is 0-50 amino acid residues; X_2 is not Ser when X_3 is Thr; and the decoy peptide or polypeptide inhibits protein phosphatase 1 (PP1) - mediated dephosphorylation of phospholamban (PLB) through competitive inhibition. It is noteworthy that the decoy peptide or polypeptide of the present invention significantly increases the phosphorylation level of PLB by inhibiting PP1-mediated dephosphorylation. Further, the decoy peptide or polypeptide provides cardio-protective effects by restoring of SERCA2a activity and inotropic effect of enhancing myocardial contractility. The present invention can contribute to the prevention or treatment of PLB-associated diseases.
Abstract translation: 本发明涉及由以下通式I表示的肽序列组成的诱饵肽或多肽：X_1-Ala-X_2-X_3-Ile-Glu-X_4（I），其中X_1为0-50个氨基酸残基; X_2是Ser，Glu或Asp; X_3是Thr，Glu或Asp; X_4为0-50个氨基酸残基; 当X_3为Thr时，X_2不为Ser; 诱饵肽或多肽通过竞争性抑制抑制蛋白磷酸酶1（PP1）介导的磷酸胆碱（PLB）的去磷酸化。 值得注意的是，本发明的诱饵肽或多肽通过抑制PP1介导的去磷酸化显着增加PLB的磷酸化水平。 此外，诱饵肽或多肽通过恢复SERCA2a活性和增强心肌收缩力的变力作用提供心脏保护作用。 本发明可有助于预防或治疗PLB相关疾病。
-
公开(公告)号：KR101223657B1
公开(公告)日：2013-01-17
申请号：KR1020110045657
申请日：2011-05-16
Applicant: 광주과학기술원
Abstract: 본 발명은 다음의 단계를 포함하는 심부전(heart failure), 섬유증 또는 염증 치료제의 스크리닝 방법에 관한 것이다: (a) Eya2(eyes absent 2) 유전자를 포함하는 세포에 시험물질을 처리하는 단계; 및 (b) 상기 Eya2 유전자의 발현을 분석하는 단계, 상기 시험물질이 Eya2 유전자의 발현을 증가시키면 심부전, 섬유증 또는 염증 예방 또는 치료제로 판단한다. 본 발명에 따르면, 본 발명은 심부전, 섬유증 또는 염증 예방 또는 치료제의 스크리닝 방법을 제공하며 Eya2 과발현은 장기간 압력과부하 하에서 심장의 섬유증 및 염증뿐 아니라, 심벽 약화, 심실 확장 및 심장 기능의 악화를 억제할 수 있다. 또한, Eya2 유전자를 유효성분으로 포함하는 본 발명의 조성물은 심부전, 섬유증 또는 염증 예방 또는 치료에 효과적이다.
-
公开(公告)号：KR101187814B1
公开(公告)日：2012-10-08
申请号：KR1020100025109
申请日：2010-03-22
Applicant: 광주과학기술원
IPC: A61K38/16 , A61P9/04 , C12N15/12 , C12N15/861
CPC classification number: A61K38/18 , A01K67/0275 , A61K31/7088 , A61K38/179 , C07K14/475 , C12N2799/022 , G01N33/6893 , G01N2800/325
Abstract: The present invention relates to a pharmaceutical composition for preventing or treating a heart failure and a method for screening a therapeutic agent for preventing or treating a heart failure. The pharmaceutical composition of the present disclosure comprises the CCN5 or CCN2”CT protein, or a genetic carrier comprising a nucleotide sequence encoding the CCN5 or the CCN2”CT protein, exhibiting dramatic prevention or treatment efficacies on a heart failure even without surgical treatments accompanied with transplanting a donor heart.
-
Patent Agency Ranking
公开(公告)号：KR1020110056273A
公开(公告)日：2011-05-26
申请号：KR1020110045657
申请日：2011-05-16
Applicant: 광주과학기술원
CPC classification number: G01N33/5308 , A61K48/00 , C12Q1/68 , C12Q2600/158 , G01N33/6893
Abstract: PURPOSE: A method for screening a therapeutic agent for heart failure, fibrosis, or inflammation is provided to suppress harmful heart remodeling and to activate PI3K/Akt/mTOR signal transduction pathway. CONSTITUTION: A method for screening a therapeutic agent for heart failure, fibrosis, or inflammation comprises: a step of treating a test material to cells containing Eya2(eyes absent 2) gene. The cells are derived from the heart. In case that the test material increases Eya2 gene expression, the test material is determined as a therapeutic agent for heart failure, fibrosis, or inflammation.
Abstract translation: 目的：提供筛选心力衰竭，纤维化或炎症治疗剂的方法，以抑制有害的心脏重塑和激活PI3K / Akt / mTOR信号转导通路。 构成：用于筛选心力衰竭，纤维化或炎症治疗剂的方法包括：将测试材料处理至含有Eya2（眼睛不存在2）基因的细胞的步骤。 细胞衍生自心脏。 在测试材料增加Eya2基因表达的情况下，测试材料被确定为心力衰竭，纤维化或炎症的治疗剂。
-
公开(公告)号：KR1020100115910A
公开(公告)日：2010-10-29
申请号：KR1020090034556
申请日：2009-04-21
Applicant: 광주과학기술원
CPC classification number: C12Q1/6813 , A61K48/00 , G01N33/6893
Abstract: PURPOSE: A composition containing Eya2 gene is provided to effectively prevent or treat heart failure, fibrosis or inflammation. CONSTITUTION: A method for screening a therapeutic agent for preventing or treating heart failure, fibrosis or inflammation comprises: a step of treating test material to cells having Eya2 gene; and a step of analyzing the expression of Eya2 gene. In case of increase of Eya2 gene expression, the test material is considered as a therapeutic agent for preventing or treating heart failure, fibrosis or inflammation. A composition for preventing or treating heart failure, fibrosis or inflammation contains Eya2 gene as an active ingredient.
Abstract translation: 目的：提供含有Eya2基因的组合物，以有效预防或治疗心力衰竭，纤维化或炎症。 构成：用于筛选用于预防或治疗心力衰竭，纤维化或炎症的治疗剂的方法包括：将试验材料处理至具有Eya2基因的细胞的步骤; 和分析Eya2基因表达的步骤。 在Eya2基因表达增加的情况下，测试材料被认为是预防或治疗心力衰竭，纤维化或炎症的治疗剂。 用于预防或治疗心力衰竭，纤维化或炎症的组合物含有Eya2基因作为活性成分。
-
公开(公告)号：KR1020100032351A
公开(公告)日：2010-03-25
申请号：KR1020090088257
申请日：2009-09-17
IPC: A61K31/381 , A61P9/04 , A61K31/496 , A61K38/10
CPC classification number: A61K31/00 , A23L33/10 , A23V2002/00 , A61K31/381 , A61K31/496 , A23V2200/326
Abstract: PURPOSE: A composition for preventing or treating heart failure is provided to change calcium sensitivity in cardiomyocytes using PKCζ(protein kinase C ζ) inhibitor. CONSTITUTION: A composition for preventing or treating heart failure contains PKCζ(protein kinase C ζ) inhibitor as an active ingredient. The PKCζ inhibitor is a compound of chemical formula I. In chemical formula I, R1 and R2 are independently alkoxycarbonyl group, substituted alkoxycarbonyl group, aryl group or substituted aryl group. At least one of R1 and R2 is alkoxycarbonyl group or substituted alkoxy carbonyl grouip.
Abstract translation: 目的：提供一种用于预防或治疗心力衰竭的组合物，以使用PKCζ（蛋白激酶Cζ）抑制剂改变心肌细胞中的钙敏感性。 构成：用于预防或治疗心力衰竭的组合物含有PKCζ（蛋白激酶Cζ）抑制剂作为活性成分。 PKCζ抑制剂是化学式I的化合物。在化学式I中，R 1和R 2独立地是烷氧基羰基，取代的烷氧基羰基，芳基或取代的芳基。 R1和R2中的至少一个是烷氧基羰基或取代的烷氧基羰基组合。
-
18.发明授权7-아미노-4-((Ｓ)-3-(4-플루오로페닐)-2-((R)-3-메틸-2-(5-메틸이소옥사졸-3-카복사미도)부탄아미도)프로판아미도)-7-옥소-2-헵테노에이트 유도체, 이의 제조방법 및 이를 유효성분으로 함유하는 바이러스성 질환의 예방 및 치료용 약학적 조성물 有权7-氨基-4-S-3-4-氟苯基-2-R-3-甲基-2,5-甲基异恶唑-3-甲酰胺丁酰氨基丙酰胺基-7-氧代-2-庚烯酸酯衍生物及其制备方法和用于 预防和治疗病毒性疾病作为活性成分
公开(公告)号：KR100891699B1
公开(公告)日：2009-04-03
申请号：KR1020070035271
申请日：2007-04-10
Applicant: 광주과학기술원
IPC: C07D261/18 , A61K31/42
Abstract: 본 발명은 7-아미노-4-((Ｓ)-3-(4-플루오로페닐)-2-((R)-3-메틸-2-(5-메틸이소옥사졸-3-카복사미도)부탄아미도)프로판아미도)-7-옥소-2-헵테노에이트 유도체, 이의 제조방법 및 이를 유효성분으로 함유하는 바이러스성 질환의 예방 및 치료용 약학적 조성물에 관한 것으로, 특히, 본 발명의 하기 화학식 1로 표시되는 유도체는 숙주세포 내로 침투한 바이러스의 복제 사이클에서 가장 중요한 역할을 하는 3C 프로테아제의 활성을 억제함으로써, 콕사키바이러스뿐만 아니라, 리노바이러스 등의 감염으로 인해 발생하는 일반 감기를 포함한 바이러스성 질환의 예방 및 치료제로 유용하게 이용될 수 있다.
[화학식 1]
(상기 식에서, R은 명세서에 정의된 바와 같다.)
콕사키바이러스, 리노바이러스, 3C 프로테아제, 저해제-
公开(公告)号：KR100436552B1
公开(公告)日：2004-06-18
申请号：KR1020010041011
申请日：2001-07-09
Applicant: 광주과학기술원
IPC: C12N15/81
Abstract: The present invention relates to a method for determining substrate specificity of a protease by employing a microorganism cotransformed with a vector expressing the protease and a vector expressing the substrate. The method comprises constructing an expression vector containing a gene for protease; constructing an expression vector containing genes for Golgi recognition signal and transmembrane domain of membrane protein locating at yeast Golgi complex, substrate domain with specific amino acid sequence, and yeast invertase; preparing a transformant by cotransforming suc2 mutant yeast with the two expression vectors; and, determining viability of the transformant in a medium containing a sole carbon source of sucrose. The invented method requires neither isolation/purification of protease nor synthesis of expensive substrate peptide. The simple and cost-effective determination of substrate specificity of proteases can be realized to allow its wide application in the identification of genes for proteases or genes for proteins cleaved with the proteases.
Abstract translation: 本发明涉及通过使用与表达蛋白酶的载体共转化的微生物和表达该底物的载体来测定蛋白酶的底物特异性的方法。 该方法包括构建含有蛋白酶基因的表达载体; 构建含高尔基体识别信号基因和位于酵母高尔基复合体的膜蛋白跨膜结构域，具有特定氨基酸序列的底物结构域和酵母转化酶的基因的表达载体; 通过将suc2突变型酵母与两种表达载体共转化来制备转化体; 并测定转化子在含有蔗糖唯一碳源的培养基中的生存力。 本发明的方法既不需要蛋白酶的分离/纯化，也不需要合成昂贵的底物肽。 可以实现蛋白酶的底物特异性的简单和成本有效的测定，以允许其广泛用于鉴定蛋白酶的基因或用蛋白酶切割的蛋白质的基因。
-
-
-
-
-
-
-
-
Search Results
19
records
(took 0.04 seconds)
