公开(公告)号：KR101412045B1

公开(公告)日：2014-06-27

申请号：KR1020130145956

申请日：2013-11-28

Applicant: 대한민국(관리부서:국립수산과학원)

Inventor： 정민민 ,  문태석 ,  한석중 ,  송홍인 ,  김재우

IPC: A01K61/00 ,  A01K63/04 ,  B01D35/027

CPC classification number: Y02A40/81 ,  A01K63/045 ,  A01K63/006 ,  B01D35/027

Abstract: The present invention provides a glass aquaculture tank equipped with a dual drainage device for aquatic organism (an organism to be cultivated and a prey organism) cultivation in a multi-stage system in order to solve the problem of the prior tank that larvae cannot be cultivated separately from others in the tank. The glass aquaculture tank equipped with a dual drainage device for aquatic organism cultivation in a multi-stage system includes an aquaculture tank main body which has an open top, a floor part in a certain size, and polygonal outer walls; a dual drainage device which is installed on an inner side of one end of the aquaculture tank main body and includes a water level adjusting pipe and a drainage pipe; and a filter fixing device installed on an inner side of the outer wall of the tank main body; a filter installed on the filter fixing device; and a larva/fry separation net which can be attached and detached on the filter fixing device. In the separate glass aquaculture tank, aquatic organisms can be separated by varying the mesh size according to the size of the cultivated organism or the type of the prey organism.

Abstract translation: 本发明提供了一种玻璃水产养殖槽，其配备有用于多级系统中的水生生物（待培养的生物体和猎物的生物体）的双重排水装置，以解决现有罐体幼虫无法栽培的问题 与坦克中的其他人分开。 在多级系统中配备用于水生生物培养的双排水装置的玻璃养殖槽包括具有开口顶部，一定尺寸的地板部分和多边形外壁的水产养殖箱主体; 双排水装置，其安装在水产养殖箱主体的一端的内侧，并包括水位调节管和排水管; 以及安装在所述箱主体的外壁的内侧的过滤器固定装置; 安装在过滤器固定装置上的过滤器; 以及可以在过滤器固定装置上安装和拆卸的幼虫/鱼苗分离网。 在单独的玻璃水产养殖罐中，可以根据培养生物体的大小或猎物生物体的类型，通过改变筛网尺寸来分离水生生物。

公开(公告)号：KR1020040105908A

公开(公告)日：2004-12-17

申请号：KR1020030037049

申请日：2003-06-10

Applicant: 대한민국(관리부서:국립수산과학원)

Inventor： 정민민 ,  민광식 ,  지영주 ,  문태석

IPC: A01K67/033

CPC classification number: A01K67/033 ,  A23K50/80

Abstract: PURPOSE: A rotifer strain for tiny feed organisms and a seawater acclimation method thereof are provided to cultivate feed organisms fed to fry of aquatic creatures in a continuously stable manner. CONSTITUTION: The rotifer strain is of the species Brachionus rotundiformis and the size ranging 80-210 micrometers and has an average lorica length of 152±25 micrometers.

Abstract translation: 目的：提供用于微量饲料生物的轮虫菌株和海水驯化方法，以持续稳定的方式培养饲喂水生生物鱼苗的饲料生物。 构成：轮虫菌株属于龙舌兰种，尺寸范围为80-210微米，平均lorica长度为152±25微米。

公开(公告)号：KR1020120065147A

公开(公告)日：2012-06-20

申请号：KR1020100126508

申请日：2010-12-10

Applicant: 재단법인 제주테크노파크

Inventor： 정용환 ,  오대주 ,  오봉세 ,  정민민 ,  이경후 ,  김지현

IPC: C12Q1/68 ,  C12N15/12

CPC classification number: C12Q1/683

Abstract: PURPOSE: A method for distinguishing Seriola quinqueradiata and Seriola lalandi is provided to specifically distinguish the fishes using mitochondria genome DNA and restriction enzyme recognition site. CONSTITUTION: A method for distinguishing Seriola quinqueradiata and Seriola lalandi by RFLP analysis comprises: a step of preparing a biological sample from Seriola quinqueradiata and Seriola lalandi; a step of isolating mitochondria genome DNA from the biological sample; a step of amplifying the DNA sequence containing a sequence of sequence number 1 for identifying Accl restriction enzyme recognition site of Seriola quinqueradiata and a DNA sequence containing sequence numbers 2 and 3 for identifying Accl restriction enzyme recognition site of Seriola lalandi; and a step of treating Accl restriction enzyme to the amplified product.

Abstract translation: 目的：提供一种区分枸杞子和Seriola lalandi的方法，用于特异性区分使用线粒体基因组DNA和限制酶识别位点的鱼类。 构成：通过RFLP分析区分枸杞子和Seriola lalandi的方法包括：从枸杞子和Seriola lalandi制备生物样品的步骤; 从生物样品中分离线粒体基因组DNA的步骤; 扩增含有序列号1序列的DNA序列的步骤，用于鉴定Seriola quinqueradiata的Accl限制酶识别位点和含有序列号2和3的DNA序列，用于鉴定Seriola lalandi的Accl限制酶识别位点; 以及将Accl限制酶处理到扩增产物的步骤。

公开(公告)号：KR101326159B1

公开(公告)日：2013-11-06

申请号：KR1020100126508

申请日：2010-12-10

Applicant: 재단법인 제주테크노파크

Inventor： 정용환 ,  오대주 ,  오봉세 ,  정민민 ,  이경후 ,  김지현

IPC: C12Q1/68 ,  C12N15/12

Abstract: 본 발명은 방어 및 부시리의 감별 방법 및 그 키트에 관한 것이다. 본 발명의 방어 및 부시리의 감별 방법 및 그 키트는 방어와 부시리의 ND5에 존재하는
Acc I 제한효소 인지 부위를 이용하거나 방어와 부시리의 미토콘드리아 게놈 DNA 서열에서 차이가 있는 서열을 이용함을 특징으로 한다.

公开(公告)号：KR101292169B1

公开(公告)日：2013-08-05

申请号：KR1020100119938

申请日：2010-11-29

Applicant: 재단법인 제주테크노파크

Inventor： 정용환 ,  오대주 ,  오봉세 ,  정민민 ,  이경후 ,  김지현

IPC: C12Q1/68 ,  C12N15/12

Abstract: 본 발명은 옥돔 및 옥두어의 감별 방법 및 그 키트에 관한 것이다. 본 발명의 옥돔 및 옥두어의 감별 방법 및 그 키트는 옥돔의 Cytochrome
b 유전자에 존재하는
Xcm I 제한효소 인식 부위와 옥두어의 Cytochrome
b 유전자의 대립 서열을 이용하거나 옥돔과 옥두어의 미토콘드리아 게놈 DNA 서열에서 차이가 있는 서열을 이용함을 특징으로 한다.

公开(公告)号：KR1020120058704A

公开(公告)日：2012-06-08

申请号：KR1020100119938

申请日：2010-11-29

Applicant: 재단법인 제주테크노파크

Inventor： 정용환 ,  오대주 ,  오봉세 ,  정민민 ,  이경후 ,  김지현

IPC: C12Q1/68 ,  C12N15/12

CPC classification number: C12Q1/683 ,  C12Q2600/156

Abstract: PURPOSE: A method for distinguishing Branchiostegus japonicus and Branchiostegus albus is provided to determine Branchiostegus japonicus and Branchiostegus albus based on mitochondria genome DNA sequence. CONSTITUTION: A method for distinguishing Branchiostegus japonicus and Branchiostegus albus using RFLP analysis comprises: a step of preparing biological sample from Branchiostegus japonicus and Branchiostegus albus; a step of isolating genome DNA of mitochondria from the biological sample; a step of amplifying DNA sequence of sequence number 1 which is a recognition site of Xcml restriction enzyme of cytochrome B gene and amplifying a DNA sequence of sequence number 2; and a step of treating with Xcml restriction enzyme.

Abstract translation: 目的：根据线粒体基因组DNA序列，提供一种鉴别芍药和Branch bus属的方法，以确定芍药和Branch。属。 构成：使用RFLP分析来区分Branch us和Branch A的方法包括：从Branch from和Branch of of of;;;;;;;;;;;;;;;; 从生物样品中分离线粒体的基因组DNA的步骤; 扩增作为细胞色素B基因的Xcml限制酶的识别位点的序列号1的DNA序列并扩增序列号2的DNA序列的步骤; 和用Xcml限制酶处理的步骤。