公开(公告)号：KR1020110017706A

公开(公告)日：2011-02-22

申请号：KR1020090075314

申请日：2009-08-14

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 예정용 ,  김현주 ,  나진주 ,  문진산 ,  권창희

IPC: C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q1/70 ,  C12Q2600/16

Abstract: PURPOSE: A method and kit for detecting rinderpest virus(RPV), peste-des-petits-ruminants virus(PPRV), blutongue virus(BTV), and rift valley fever virus(RVFV) is provided to diagnose four diseases with less labor. CONSTITUTION: A primer set for amplifying F gene of rinderpest virus(RPV) has a primer having base sequences of sequence numbers 1 and 2. A primer set for amplifying F gene of Peste-des-petits-ruminants virus(PPRV) has a primer having sequences of sequence numbrs 3-6. A primer set for amplifying VP3 gene of bluetongue virus(BTV) has a primer having sequences of sequence numbers 7-15. A primer set for amplifying NS gene of rift valley fever virus(VFV) has a primer having sequences of sequence numbers 16-19. A method for detecting the presence of virus comprise: a step of extracting RNA from a sample having virus; and a step of performing multiple RT-PCR.

Abstract translation: 目的：提供用于检测牛瘟病毒（RPV），瘟热反刍动物病毒（PPRV），bl病病毒（BTV）和裂谷热病毒（RVFV）的方法和试剂盒，以诊断较少劳动的四种疾病。 构成：用于扩增牛瘟病毒（RPV）F基因的引物组具有具有序列号1和2的碱基序列的引物。用于扩增Peste-des-petit-ruminants病毒（PPRV）的F基因的引物组具有引物 具有序列号3-6的序列。 用于扩增蓝舌病毒（BTV）的VP3基因的引物组具有序列号为7-15的引物。 用于扩增裂谷热病毒（VFV）的NS基因的引物组具有序列号为16-19的引物。 用于检测病毒存在的方法包括：从具有病毒的样品中提取RNA的步骤; 以及进行多次RT-PCR的步骤。

公开(公告)号：KR1020100105974A

公开(公告)日：2010-10-01

申请号：KR1020090024360

申请日：2009-03-23

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김현주 ,  예정용 ,  문진산 ,  나진주 ,  권창희

IPC: G01N33/53 ,  G01N33/535 ,  C07K16/08

Abstract: PURPOSE: A competitive ELISA using a monoclonal antibody to a recombinant N protein is provided to shorten diagnosis time. CONSTITUTION: A competitive ELISA using recombinant N protein and monoclonal antibody 1-59 is used for diagnosing rift valley fever. A method for diagnosing rift valley fever comprises: a step of attaching a recombinant N protein antigen of rift valley fever virus on an ELISA plate; a step of removing the recombinant N protein antigen which is not attached on the plate; a step of competing 1-59 monoclonal antibodies to the recombinant N protein; and a step of reacting the secondary antibody to the monoclonal antibody conjugated with a target material.

Abstract translation: 目的：提供使用重组N蛋白单克隆抗体的竞争ELISA，以缩短诊断时间。 构成：使用重组N蛋白和单克隆抗体1-59的竞争性ELISA用于诊断裂谷热。 用于诊断裂谷热的方法包括：在ELISA板上附着裂谷热病毒的重组N蛋白抗原的步骤; 除去未附着在板上的重组N蛋白抗原的步骤; 与重组N蛋白竞争1-59单克隆抗体的步骤; 以及使二次抗体与与靶材料缀合的单克隆抗体反应的工序。