메타리지움 애니소플리애를 이용한 꿀벌응애 구제용 조성물 및 이를 이용한 방제 방법
    2.
    发明公开
    메타리지움 애니소플리애를 이용한 꿀벌응애 구제용 조성물 및 이를 이용한 방제 방법 有权
    用异硫氰酸酯控制VARROA分解剂的组合物及其使用

    公开(公告)号:KR1020130077254A

    公开(公告)日:2013-07-09

    申请号:KR1020110145861

    申请日:2011-12-29

    CPC classification number: A01N63/04 A01N25/02 A01N25/06 C12N1/14 C12R1/645

    Abstract: PURPOSE: A composition for controlling varroa destructor using metarizium anisopliae and a control method using the same are provided to more effectively control varroa destructor by selecting effective strains. CONSTITUTION: A composition for controlling varroa destructor includes Metarizium anisopliae ATCC16085. The spore number of the Metarizium anisopliae ATCC16085 strain is 10^5-10^10/ml. The composition for controlling varroa destructor includes Metarizium anisopliae KTCC40029. The spore number of the Metarizium anisopliae KTCC40029 strain is 10^5-10^10/ml. A control method of varroa destructor includes a step of spraying a diluted spore fluid of the Metarhizium anisopliae ATCC16085 strain or KTCC40029 strain.

    Abstract translation: 目的:提供一种使用异色for for控制变色析构体的组合物和使用其的控制方法,以通过选择有效菌株更有效地控制变种析出物。 构成:用于控制变性沉淀物的组合物包括Metarizium anisopliae ATCC16085。 Metarizium anisopliae ATCC16085菌株的孢子数为10 ^ 5-10 ^ 10 / ml。 用于控制varroa析出物的组合物包括Metarizium anisopliae KTCC40029。 Metthizium anisopliae KTCC40029菌株的孢子数为10 ^ 5-10 ^ 10 / ml。 变色析晶体的控制方法包括喷洒Metahizium anisopliae ATCC16085株或KTCC40029菌株的稀释孢子液的步骤。

    수포성 구내염 뉴저지형 유전자 재조합 당단백질 제작 및 그의 용도
    4.
    发明公开
    수포성 구내염 뉴저지형 유전자 재조합 당단백질 제작 및 그의 용도 有权
    一种基因工程乙型肝炎病毒血清新病毒及其应用的构建

    公开(公告)号:KR1020110034154A

    公开(公告)日:2011-04-05

    申请号:KR1020090091578

    申请日:2009-09-28

    Abstract: PURPOSE: A method for diagnosing vesicular stomatitis virus serotype New Jersey is provided to detect New Jersey specific antibody in the livestock serum. CONSTITUTION: A gene recombinant baculovirus expression vector contains a gene of sequence number 1 vesicular stromatitis virus serotype New Jersey protein. A method for preparing the glycoprotein comprises: a step of co-infecting a vector and baculovirus gene to a host cell; a step of culturing the infected host cell and separating supernatant containing the recombinant baculovirus from the culture; a step of infecting the host cell with the supernatant; a step of collecting cell pellet and crushing the cells; and a step of isolating the glycoprotein from the crushed cell culture. The baculovirus gene is pBacPAK6. The host cell is an insect cell.

    Abstract translation: 目的:提供一种用于诊断水泡性口炎病毒血清型新泽西的方法,用于检测家畜血清中的新泽西特异性抗体。 构成:基因重组杆状病毒表达载体含有序列号1的囊泡性间质炎病毒血清型新泽西蛋白的基因。 制备糖蛋白的方法包括:将宿主细胞共同感染载体和杆状病毒基因的步骤; 培养受感染的宿主细胞并从培养物中分离含有重组杆状病毒的上清液的步骤; 用上清液感染宿主细胞的步骤; 收集细胞沉淀并粉碎细胞的步骤; 以及从破碎的细胞培养物中分离糖蛋白的步骤。 杆状病毒基因是pBacPAK6。 宿主细胞是昆虫细胞。

    라텍스 비드를 이용한 네오스포라병의 진단 시약 및 진단키트
    5.
    发明授权
    라텍스 비드를 이용한 네오스포라병의 진단 시약 및 진단키트 有权
    诊断代理和使用LATEX珠子的新生儿手套

    公开(公告)号:KR100951056B1

    公开(公告)日:2010-04-07

    申请号:KR1020070109076

    申请日:2007-10-29

    Abstract: 본 발명은 라텍스 비드를 이용한 네오스포라병의 진단 시약 및 진단 키트에 관한 것이다. 보다 구체적으로 본 발명은 네오스포라 캐니눔(
    Neospora

    caninum ) 항원으로 감작시킨 라텍스 비드를 포함하는 네오스포라병 진단 시약, 이를 포함하는 네오스포라병 진단 키트 및 이를 이용한 네오스포라병 진단 방법에 관한 것이다.
    본 발명에 따른 네오스포라 원충 항원 및 라텍스 비드를 이용한 진단 시약은 네오스포라병에 대한 정확한 진단을 고가의 장비없이 간편하게 수행할 수 있는 효과가 있다.
    네오스포라병, 라텍스 비드, 진단

    곤충세포 발현 재조합 3ABC 비구조단백질 항원 및 단클론항체를 이용한 구제역 진단방법
    9.
    发明公开
    곤충세포 발현 재조합 3ABC 비구조단백질 항원 및 단클론항체를 이용한 구제역 진단방법 有权
    使用在INSEC细胞和单克隆抗体中表达的重组蛋白3ABC非结构蛋白的足球疾病的诊断方法

    公开(公告)号:KR1020030052858A

    公开(公告)日:2003-06-27

    申请号:KR1020010082976

    申请日:2001-12-21

    Abstract: PURPOSE: Diagnostic methods of foot-and-mouth disease using recombinant 3ABC non-structural protein expressed in insect cells and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant baculovirus to be expressed in insect cells is prepared by co-transfection with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed by infection of the insect cells with the recombinant baculovirus. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the diluate on a plate; (2) reacting the testing serum with the plate; (3) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (4) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.

    Abstract translation: 目的:提供使用在昆虫细胞中表达的重组3ABC非结构蛋白和单克隆抗体的口蹄疫诊断方法,从而比现有方法更快速,准确地诊断口蹄疫。 构成:编码来自韩国口蹄疫病毒O / SKR / 2000的重组体3ABC非结构蛋白的基​​因具有SEQ ID NO:1的核苷酸序列。在昆虫细胞中表达的重组杆状病毒通过 用含有SEQ ID NO:1的重组3ABC非结构蛋白基因的重组载体共转染。重组体3ABC非结构蛋白用重组杆状病毒感染昆虫细胞表达。 通过将在大肠杆菌中表达的重组3ABC非结构蛋白质导入动物中,从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合来制备杂交瘤细胞系3F-11(KCTC 10138BP)。 从杂交瘤细胞系3F-11(KCTC 10138BP)产生单克隆抗体。 口蹄疫的诊断方法包括以下步骤:(1)在包被缓冲溶液中稀释重组体3ABC非结构蛋白,将稀释液倒入平板上; (2)使测试血清与板反应; (3)使与测试血清中的口蹄疫病毒抗体结合并具有酶,放射性物质或荧光材料的缀合物与测试血清反应; 和(4)测量酶反应的强度,荧光反应或与缀合物的辐射反应。

    돼지 대장균 설사증 예방 및 치료용 특이항체를 포함한 난황건조분말을 이용한 사료첨가제
    10.
    发明公开
    돼지 대장균 설사증 예방 및 치료용 특이항체를 포함한 난황건조분말을 이용한 사료첨가제 无效
    饲料添加剂使用具有防腐和治疗猪苓蓉的干酪粉

    公开(公告)号:KR1020010037612A

    公开(公告)日:2001-05-15

    申请号:KR1019990045242

    申请日:1999-10-19

    CPC classification number: A23K50/00 A23K10/20 A23K40/10

    Abstract: PURPOSE: A feed additive is provided to prevent and treat the pig colon bacillus diarrhea by using dried yolk powder having an idioantibody. CONSTITUTION: A testing vaccine manufactured by using a cilium antigen of the colon bacillus is inoculated on a layer and immunized. The eggs are disinfected with alcohol and the egg yolk is aseptically collected in an Erlenmeyer Flask. The collected yolk is injected into a powder dryer to gather yolk powder. The injecting temperature of the powder dryer is 140deg.C and the releasing temperature is 72deg.C. The dried powder weight per 1ml vol and the moisture content of the egg yolk are 0.38g and 2%, respectively. The value of an antibody is rarely changed before and after the drying.

    Abstract translation: 目的:提供饲料添加剂,通过使用具有不良反应的干燥的蛋黄粉来预防和治疗猪大肠杆菌腹泻。 构成:将通过使用大肠杆菌的纤毛抗原制造的测试疫苗接种在一层上并免疫。 鸡蛋用酒精消毒,蛋黄被无菌地收集在锥形瓶中。 将收集的蛋黄注入粉末干燥器中以收集蛋黄粉末。 粉末干燥机的注射温度为140℃,脱模温度为72℃。 每1ml体积的干燥粉末重量和蛋黄的水分含量分别为0.38g和2%。 干燥前后抗体的值很少变化。

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