公开(公告)号：KR1020160036189A

公开(公告)日：2016-04-04

申请号：KR1020140127919

申请日：2014-09-24

Applicant: 대한민국(농촌진흥청장)

Inventor： 이상훈 ,  이기원 ,  박형수 ,  최기준 ,  김기용 ,  지희정

IPC: A01H5/00 ,  C07K14/415 ,  C12N15/29 ,  C12N15/82

CPC classification number: A01H5/00 ,  C07K14/415 ,  C12N15/09 ,  C12N15/82 ,  C12Q1/6827

Abstract: 본발명은비생물적스트레스내성관련 EsHsp16.9 (L. Heat shock protein 16.9) 단백질또는이를코딩하는유전자및 이를이용하여식물의비생물적스트레스에대한내성을증진시키는방법에관한것이다. 본발명의 Eshsp16.9 유전자는염, 기근, 또는중금속과같은다양한비생물적스트레스환경에서발현이증가한다. 따라서, Eshsp16.9 유전자는목초의분자육종에이용되어비생물적스트레스내성이강한형질전환식물체를개발하는데유용하게사용될수 있다.

Abstract translation: 本发明涉及非生物胁迫耐受相关的EsHsp16.9（Elymus sibiricus L.热休克蛋白16.9）蛋白或其编码基因，以及使用其的植物对非生物胁迫的耐受性的方法。 根据本发明，在各种非生物胁迫环境如盐，饥荒或重金属中，Eshsp16.9基因的表达增加。 因此，Eshsp16.9基因用于草的分子育种，可用于开发具有强非生物胁迫耐受性的转基因植物。

公开(公告)号：KR1020110124606A

公开(公告)日：2011-11-17

申请号：KR1020100044094

申请日：2010-05-11

Applicant: 한국화학연구원 ,  대한민국(농촌진흥청장)

Inventor： 이상훈 ,  김진석 ,  김기용 ,  박형수 ,  임영철 ,  이기원 ,  최정섭

IPC: A01H5/00 ,  C12N15/29 ,  C12N15/82 ,  A01H5/10

CPC classification number: C12N15/8274 ,  A01N57/20 ,  C12N15/8205 ,  C12N15/8223

Abstract: PURPOSE: A method for producing antibiotics marker-free/herbicide complex resistant creeping bentgrass plant is provided to improve weed herbicidal effect and to enhance creeping bentgrass productivity. CONSTITUTION: A method for producing antibiotics marker-free/herbicide complex resistant creeping bentgrass plant comprises: a step of transforming Agrobacterium with a plant expression vector containing herbicide resistant bar gene and CP4-EPSPS(P4 5-enolpyruvylshikimate-3- phosphate synthase) gene without an antibiotic marker gene; a step of co-culturing the transformed Agrobacteriuam and creeping bentgrass embryogenic callus; a step of selecting new shoots transformed from the embryogenic callus and growing; and a step of testing herbicide resistance using glufosinate ammonium and glyphosate. The plant expression vector is p35S-EPSPS-B vector.

Abstract translation: 目的：提供抗生素无标记/除草剂复合抗性爬行弯筋草植物的生产方法，以提高杂草除草效果，提高草坪草的生长。 构成：生产抗生素无标记/除草剂复合抗性爬行弯曲草植物的方法包括：用含有除草剂抗性bar基因和CP4-EPSPS（P4-5-烯醇丙酮酸莽草酸-3-磷酸合成酶）基因的植物表达载体转化土壤杆菌的步骤 没有抗生素标记基因; 共同培养转化的土壤杆菌和爬行的弯曲草胚发生愈伤组织的步骤; 选择从胚发生的愈伤组织转化并生长的新芽的步骤; 以及使用草铵膦和草甘膦测试除草剂抗性的步骤。 植物表达载体为p35S-EPSPS-B载体。

公开(公告)号：KR101757621B1

公开(公告)日：2017-07-18

申请号：KR1020150155226

申请日：2015-11-05

Applicant: 대한민국(농촌진흥청장)

Inventor： 이기원 ,  이상훈 ,  박형수 ,  김기용 ,  지희정 ,  황태영 ,  정종성

IPC: C12N15/82 ,  C07K14/415 ,  A01H5/00

Abstract: 본발명은비생물적스트레스내성관련 EsHsp90.1 (L. Heat shock protein 90.1) 단백질또는이를코딩하는유전자및 이를이용하여비생물적스트레스에대한내성을증진시키는방법에관한것이다. 본발명의 EsHsp90.1 유전자는고온, 염, 기근, 또는중금속과같은다양한비생물적스트레스환경에서발현이증가한다. 따라서, EsHsp90.1 유전자는목초의분자육종에이용되어비생물적스트레스내성이강한형질전환체를개발하는데유용하게사용될수 있다.

公开(公告)号：KR1020140011868A

公开(公告)日：2014-01-29

申请号：KR1020120079517

申请日：2012-07-20

Applicant: 대한민국(농촌진흥청장)

Inventor： 이상훈 ,  박형수 ,  이기원 ,  최기준 ,  김기용 ,  지희정 ,  서성

IPC: A01H4/00 ,  C12N5/04 ,  C12N5/02

CPC classification number: A01H4/005 ,  A01C1/08 ,  A01G20/00 ,  A01H4/001 ,  A01H4/008 ,  C12N5/0025 ,  C12N2500/34

Abstract: The present invention provides: a mass-production method of a tape grass plant body using the induction of callus from grown seeds of tape grass and the redifferentiation of shoots; the tape grass plant body produced by the method; and a medium composition for the induction of callus from the tape grass and the redifferentiation of the shoots.

Abstract translation: 本发明提供一种使用来自带状草的种子的愈伤组织的诱导和芽的再分化的带草植物体的批量生产方法; 通过该方法生产的胶带草植物体; 以及用于诱导来自带草的愈伤组织和嫩芽再分化的培养基组合物。

公开(公告)号：KR1020130054478A

公开(公告)日：2013-05-27

申请号：KR1020110117365

申请日：2011-11-11

Applicant: 대한민국(농촌진흥청장) ,  한국생명공학연구원

Inventor： 이상훈 ,  이기원 ,  최기준 ,  김기용 ,  지희정 ,  임영철 ,  김차영 ,  곽상수 ,  전효곤

IPC: A01H5/10 ,  C12N15/82 ,  C12N15/29 ,  A23K1/16

CPC classification number: C12N15/825 ,  A23K10/30 ,  C07K14/415 ,  C12N15/8205 ,  C12N15/8257

Abstract: PURPOSE: A system for preparing a large amount of anthocyanin is provided to develop a health functional food industry, a cosmetic industry, a medical product industry, and a dye industry. CONSTITUTION: IbMYB1a gene has a base sequence of sequence number 1. A method for producing a transgenic plant with enhanced anthocyanin content comprises: a step of transforming a plant cell with a recombinant vector containing Ipomoea batatas-derived IbMYB1a gene; and a step of redifferentiating a plant from the plant cells. The plant is dicotyledones. A composition for enhancing anthocyanin in a plant contains IbMYB1a gene.

Abstract translation: 目的：提供一种制备大量花青素的系统，用于开发健康功能食品工业，化妆品工业，医疗产品工业和染料工业。 构成：IbMYB1a基因具有序列号1的碱基序列。具有增加的花青素含量的转基因植物的制备方法包括：用含有番薯as源的IbMYB1a基因的重组载体转化植物细胞的步骤; 以及从植物细胞重新分配植物的步骤。 植物是双子叶植物。 用于增强植物中花青素的组合物含有IbMYB1a基因。

公开(公告)号：KR1020100015187A

公开(公告)日：2010-02-12

申请号：KR1020080076124

申请日：2008-08-04

Applicant: 대한민국(농촌진흥청장) ,  성균관대학교산학협력단

Inventor： 김종범 ,  한범수 ,  오상수 ,  강한철 ,  김용환 ,  박종석 ,  하선화 ,  김기용 ,  성병렬 ,  송재영 ,  김병한 ,  임성인 ,  양주성 ,  배지영

IPC: C12N15/12 ,  C12N15/09

Abstract: PURPOSE: A novel cholera vaccine containing an E2 antigen gene or transgenic plant expressing the gene is provided to produce an antibody to the E2 antigen and prevent cattle sickness. CONSTITUTION: An E2 antigen gene of swine cholera protein contains ER-tagged sequence or ER-retention sequence. The E2 antigen gene contains a sequence of sequence number 1. An E2 antigen protein contains amino acid sequence of sequence number 2. An expression vector for transforming a plant which is able to express E2 antigen contains the E2 antigen gene. The expression vector is E2/pB7WG2D(deposit number KACC 95087P). A plant which expresses the E2 antigen gene includes crop for livestock feed.

Abstract translation: 目的：提供含有E2抗原基因或表达该基因的转基因植物的新型霍乱疫苗，以产生E2抗原的抗体并防止牛病。 构成：猪霍乱蛋白的E2抗原基因含有ER标记的序列或ER-保留序列。 E2抗原基因含有序列号1的序列.E2抗原蛋白含有序列号2的氨基酸序列。用于转化能够表达E2抗原的植物的表达载体含有E2抗原基因。 表达载体为E2 / pB7WG2D（保藏号KACC 95087P）。 表达E2抗原基因的植物包括家畜饲料作物。