Abstract:
PURPOSE: A manufacturing method of transformed potato plant with multifariousness virus resistance and a transformed potato plant manufactured by using the same are provided to increase potato productivity and to be used as a virus resistance parent. CONSTITUTION: A transformed potato plant is obtained by being transformed into an expression vector pK7GWIWG 2 which includes a gene fusing more than 3 kinds of virus genes selected from potato mop-top virus gene of the sequence number 5, PVY(Potato virus Y) gene of the sequence number 1(SEQ ID NO:1), PLRV(Potato leafroll virus) gene of the sequence number 2,PVA(Potato virus A) gene of the sequence number 3, or TRV(Tobacco rattle virus) gene of the sequence number 4 in sense and anti-sense direction. A manufacturing method of the multifariousness virus resistant transformed potato plant comprises the following steps: transforming Agrobacterium tumefaciens into an expression vector pK7GWIWG 2(I)/ YLRATRPM including YLRATRPM gene of the sequence number 6; and transforming potato cells by cocltivating agrobacterium and potato cells.
Abstract:
PURPOSE: A primer for detecting orchid virus and a detection method using the same are provided to simply detect infection by ORSV, CyMV, and OFV. CONSTITUTION: A primer set for detecting orchid virus contains: ORSV-420F primer of sequence number 1; ORSV-420R primer of sequence number 2; CyMV-620F primer of sequence number 3; CyMV-620R primer of sequence number 4, OFV-873F primer of sequence number 5; or OFV-873R primer of sequence number 6. A method for detecting orchid virus comprises: a step of performing RT-PCR and PCR using the primer and RNA isolated from a sample; and a step of determining the detection by the virus through the size of PCR products.
Abstract:
본 발명은 Potato virus Y(PVY), Potato leafroll virus(PLRV), Potato virus A(PVA), Tobacco rattle virus(TRV) 및 Potato mop-top virus(PMTV)의 5종의 식물 바이러스 각각으로부터의 유전자 단편을 융합시켜 제작한 융합유전자(YLRATRPM) 또는 융합유전자(YLRA)를 포함하는 재조합 발현 벡터로 감자 세포를 형질전환 시킴으로써 상기 융합유전자 제작에 이용된 상기 바이러스 5종 또는 3종 모두 에 대하여 저항성을 나타내는 형질전환 감자식물체의 제조 방법 및 상기 방법으로 제조된 형질전환 감자식물체에 관한 것이다.
Abstract:
PURPOSE: A method for determining tomato spotted wilt virus resistance using tomato spotted wilt virus is provided to evaluate resistance of improved Capsicum annuum and tomato species. CONSTITUTION: A method for determining tomato spotted wilt virus resistance in Capsicum annuum species comprises: a step of isolating RNA from a target Capsicum annuum species and preparing cDNA; a step of performing PCR using a PCR primer set corresponding to DNA encoding one nucleocapsid protein among sequence numbers 1-5; and a step of determining the presence of sequences corresponding to the sequence numbers 1-5.
Abstract:
PURPOSE: A duplex primer for diagnosing INSV and TSWV is provided to specifically diagnose INSV and TSWV and to enhance detection limit. CONSTITUTION: A duplex primer for diagnosing INSV(impatiens necrotic spot virus) and TSWV(tomato spotted wilt virus) contains oligonucleotide primer set containing an oligonucleotide with 15 or more continuous nucleotide fragment in sequence number 1 and 15 or more continuous nucleotide fragment in sequence number 2. A kit for simultaneously diagnosing INSV and TSWV contains the primer set and reagent for reverse-transcription and amplification.