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1.发明公开멜론괴저반점바이러스(ＭＮＳＶ)의 멜론 분리주 및 수박 분리주의 동시진단 프라이머 세트 및 이를 포함하는 진단 키트 有权用于检测来自梅隆和水的梅隆NECROTIC SPOT病毒的检测装置及其包装盒
公开(公告)号：KR1020120061449A
公开(公告)日：2012-06-13
申请号：KR1020100122770
申请日：2010-12-03
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/68 , C12Q2600/16
Abstract: PURPOSE: A primer set for diagnosing MNSV from melon and watermelon and a kit are provided to definitely diagnose MNSV developed in melon and watermealon. CONSTITUTION: A primer set for diagnosing MNSV from melon and watermelon is denoted by sequence numbers 1 and 2. A primer set for simultaneously diagnosing MNSV from melon and watermelon has a primer set of sequence numbers 1 and 2 and sequence number 3 and 4. The primer set has a primer set of sequence numbers 1 and 2 and sequence number 5 and 6. A kit for diagnosing MNSV from melon and watermelon contains the primer set.
Abstract translation: 目的：用于诊断来自西瓜和西瓜的MNSV的引物组和试剂盒，以确定诊断在瓜和威瓜中开发的MNSV。 构成：用于从瓜类和西瓜诊断MNSV的引物组由序列号1和2表示。用于同时诊断来自甜瓜和西瓜的MNSV的引物组具有序列号1和2以及序列号3和4的引物组。 引物组具有序列号1和2以及序列号5和6的引物组。用于诊断来自瓜和西瓜的MNSV的试剂盒含有引物组。
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公开(公告)号：KR1020110072681A
公开(公告)日：2011-06-29
申请号：KR1020090129716
申请日：2009-12-23
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6844 , C12Q2521/101 , C12Q2521/107 , C12Q2527/101 , C12Q2537/143
Abstract: PURPOSE: A duplex primer for diagnosing INSV and TSWV is provided to specifically diagnose INSV and TSWV and to enhance detection limit. CONSTITUTION: A duplex primer for diagnosing INSV(impatiens necrotic spot virus) and TSWV(tomato spotted wilt virus) contains oligonucleotide primer set containing an oligonucleotide with 15 or more continuous nucleotide fragment in sequence number 1 and 15 or more continuous nucleotide fragment in sequence number 2. A kit for simultaneously diagnosing INSV and TSWV contains the primer set and reagent for reverse-transcription and amplification.
Abstract translation: 目的：提供诊断INSV和TSWV的双引物，专门诊断INSV和TSWV，提高检测限。 构成：用于诊断INSV（凤仙桃坏死斑点病毒）和TSWV（番茄斑点枯萎病毒）的双链体引物含有寡核苷酸引物组，其含有序列号为15以上的连续核苷酸片段的15个以上连续核苷酸片段的寡核苷酸，15个以上序列号的连续核苷酸片段 2.用于同时诊断INSV和TSWV的试剂盒含有用于逆转录和扩增的引物组和试剂。
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公开(公告)号：KR1020140109050A
公开(公告)日：2014-09-15
申请号：KR1020130023217
申请日：2013-03-05
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6844 , C12Q1/6888 , C12Q1/701 , C12Q2561/113 , C12Q2600/16
Abstract: Provided is a single tube-multiplex PCR primer set for diagnosing Tomato yellow leaf curl virus (TYLCV) comprising a PCR primer set of sequence number 1 to 2 and a PCR primer set of sequence number 3 to 4 to enable accurate diagnosis of TYLCV using tomato juice without separating nucleic acid of tomato by developing one primer set specifically detecting TYLCV infecting tomatoes and one tomato β-tubulin gene primer set for determining whether or not PCR works normally.
Abstract translation: 提供了一种用于诊断番茄黄叶卷曲病毒（TYLCV）的单管多重PCR引物组，其包含序列号1至2的PCR引物组和序列号3至4的PCR引物组，以使得能够使用番茄准确诊断TYLCV 通过开发一种特异性检测TYLCV感染番茄的引物组和一种用于确定PCR是否正常工作的番茄β-微管蛋白基因引物组，不分离番茄核酸的果汁。
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公开(公告)号：KR101349087B1
公开(公告)日：2014-01-20
申请号：KR1020110039560
申请日：2011-04-27
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 토마토반점위조바이러스 및 이의 용도에 관한 것이다. 보다 자세하게는 서열번호 1 내지 5 중 어느 하나의 뉴클레오 캡시드 단백질(nucleocapsid protein)를 코딩하는 RNA를 포함하는 토마토 반점위조바이러스에 관한 것이다. 본 발명의 신규한 바이러스를 통하여, 품종개량 및 개량된 품종에 대한 저항성을 평가할 수 있을 것이다.
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公开(公告)号：KR1020130104078A
公开(公告)日：2013-09-25
申请号：KR1020120025233
申请日：2012-03-12
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6895 , C12N15/8205 , C12N15/8283 , C12Q2600/13
Abstract: PURPOSE: A method for determining a variety with resistance to tomato yellow leaf curl virus (TYLCV) is provided to enhance convenience and precision in resistivity evaluation and to cheaply and repetitively evaluate resistance. CONSTITUTION: A method for determining a variety with resistance to TYLCV comprises the step of infecting plant leaves with TYLCV strains. The method also comprises the step of measuring TYLCV-Ct/Actin-Ct value. The method additionally comprises the step of determining a resistant variety when the TYLCV-Ct/Actin-Ct value is 0.7 or greater. TYLCV includes at least three among TYLCV-IS, TYLCV-NS, TYLCV-YS, TYLCV-GJ, and TYLCV-Cgh. [Reference numerals] (AA) Bacchus; (BB) Supper sunroad; (CC) Ten ten
Abstract translation: 目的：提供一种测定番茄黄叶卷曲病毒（TYLCV）耐药性的方法，以提高电阻率评估的便利性和精确性，并且便宜且重复地评估抗性。 构成：用于确定对TYLCV具有抗性的品种的方法包括用TYLCV毒株感染植物叶的步骤。 该方法还包括测量TYLCV-Ct / Actin-Ct值的步骤。 该方法还包括当TYLCV-Ct / Actin-Ct值为0.7或更大时确定抗性品种的步骤。 TYLCV包括TYLCV-IS，TYLCV-NS，TYLCV-YS，TYLCV-GJ和TYLCV-Cgh中的至少三个。 （附图标记）（AA）酒神; （BB）晚餐太阳; （CC）十十
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Patent Agency Ranking6.发明授权멜론괴저반점바이러스(ＭＮＳＶ)의 멜론 분리주 및 수박 분리주의 동시진단 프라이머 세트 및 이를 포함하는 진단 키트 有权用于检测来自梅隆和水的梅隆NECROTIC SPOT病毒的检测装置及其包装盒
公开(公告)号：KR101248059B1
公开(公告)日：2013-03-27
申请号：KR1020100122770
申请日：2010-12-03
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 멜론괴저반점바이러스(MNSV)의 멜론 분리주 및 수박 분리주의 동시진단 프라이머 세트, 이를 포함하는 진단 키트 및 이를 이용한 진단방법에 관한 것이다. 보다 자세하게는 서열번호 1 및 2로 표시되는 멜론괴저반점바이러스(MNSV)의 멜론 분리주 및 수박 분리주의 동시진단 프라이머 세트를 통하여 멜론 분리주 및 수박 분리주를 명확하게 구분하여 진단할 수 있다.
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公开(公告)号：KR1020110049513A
公开(公告)日：2011-05-12
申请号：KR1020090106557
申请日：2009-11-05
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6844 , C12N15/11 , C12Q1/70
Abstract: PURPOSE: A primer for detecting orchid virus and a detection method using the same are provided to simply detect infection by ORSV, CyMV, and OFV. CONSTITUTION: A primer set for detecting orchid virus contains: ORSV-420F primer of sequence number 1; ORSV-420R primer of sequence number 2; CyMV-620F primer of sequence number 3; CyMV-620R primer of sequence number 4, OFV-873F primer of sequence number 5; or OFV-873R primer of sequence number 6. A method for detecting orchid virus comprises: a step of performing RT-PCR and PCR using the primer and RNA isolated from a sample; and a step of determining the detection by the virus through the size of PCR products.
Abstract translation: 目的：提供用于检测兰花病毒的引物和使用其的检测方法，以简单地检测ORSV，CyMV和OFV的感染。 构成：用于检测兰花病毒的引物组含有：序列号1的ORSV-420F引物; 序列号2的ORSV-420R引物; 序列号3的CyMV-620F引物; 序列号4的CyMV-620R引物，序列号5的OFV-873F引物; 或序列号6的OFV-873R引物。用于检测兰花病毒的方法包括：使用从样品分离的引物和RNA进行RT-PCR和PCR的步骤; 以及通过PCR产物的大小确定病毒检测的步骤。
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公开(公告)号：KR1020120121639A
公开(公告)日：2012-11-06
申请号：KR1020110039560
申请日：2011-04-27
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q2600/13
Abstract: PURPOSE: A method for determining tomato spotted wilt virus resistance using tomato spotted wilt virus is provided to evaluate resistance of improved Capsicum annuum and tomato species. CONSTITUTION: A method for determining tomato spotted wilt virus resistance in Capsicum annuum species comprises: a step of isolating RNA from a target Capsicum annuum species and preparing cDNA; a step of performing PCR using a PCR primer set corresponding to DNA encoding one nucleocapsid protein among sequence numbers 1-5; and a step of determining the presence of sequences corresponding to the sequence numbers 1-5.
Abstract translation: 目的：提供一种利用番茄斑点枯萎病毒测定番茄斑点枯萎病毒抗性的方法，以评估改良辣椒和番茄种类的抗性。 构成：在辣椒种中测定番茄斑点枯萎病毒抗性的方法包括：从目标辣椒种分离RNA并制备cDNA的步骤; 使用与编码序列号1-5中的一个核衣壳蛋白的DNA对应的PCR引物组进行PCR的步骤; 以及确定对应于序列号1-5的序列的存在的步骤。
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公开(公告)号：KR101153458B1
公开(公告)日：2012-06-27
申请号：KR1020090129716
申请日：2009-12-23
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A duplex primer for diagnosing INSV and TSWV is provided to specifically diagnose INSV and TSWV and to enhance detection limit. CONSTITUTION: A duplex primer for diagnosing INSV(impatiens necrotic spot virus) and TSWV(tomato spotted wilt virus) contains oligonucleotide primer set containing an oligonucleotide with 15 or more continuous nucleotide fragment in sequence number 1 and 15 or more continuous nucleotide fragment in sequence number 2. A kit for simultaneously diagnosing INSV and TSWV contains the primer set and reagent for reverse-transcription and amplification.
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公开(公告)号：KR101491788B1
公开(公告)日：2015-02-13
申请号：KR1020130023217
申请日：2013-03-05
Applicant: 대한민국(농촌진흥청장)
Abstract: 토마토에 발생하는 TYLCV를 특이적으로 진단할 수 있는 프라이머 1종 세트와 PCR의 정상 작동 유무를 판단할 수 있는 토마토 베타튜불린 (β-tubulin) 유전자 프라이머 1종 세트를 개발하여 토마토의 핵산 분리 없이 토마토 즙액을 가지고 바로 TYLCV의 진단을 정확하게 가능하도록 하고자 서열목록 1 내지 2의 PCR 프라이머 세트 및 서열번호 3 내지 4의 PCR 프라이머 세트로 구성된 토마토황화잎말림 바이러스 진단을 위한 단일 튜브-멀티플렉스 PCR 프라이머 세트를 제공한다.
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