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公开(公告)号：KR1020130046342A
公开(公告)日：2013-05-07
申请号：KR1020120080601
申请日：2012-07-24
Applicant: 서울대학교산학협력단
CPC classification number: C12P19/34 , B01J2219/00315 , B01J2219/00441 , B01J2219/00468 , B01J2219/005 , B01J2219/00596 , B01J2219/00648 , B01J2219/00691 , B01J2219/00693 , B01J2219/00722 , C40B20/02 , C40B50/14 , C40B60/04 , C12N15/1006 , C12Q1/6806
Abstract: PURPOSE: A method for producing a large amount of high purity nucleotides is provided to quickly and accurately isolated microbeads with a predetermined base sequence and to amplify in a usable amount. CONSTITUTION: A method for producing a large amount of high purity nucleotides comprises: a step of providing a sequencing substrate with a replication library of oligonucleotides on a solid support(S110); a step of sequencing the replication library(S120); a step of obtaining measured location information of the solid support on the sequencing substrate(S130); a step of mapping pixel information and measured location information generated from the solid support(S140); a step of isolating a solid support with desired base sequence from the sequencing substrate using the mapping result(S150); and a step of amplifying the oligonucleotide of the isolated solid support(S160). [Reference numerals] (AA) Start; (BB) End; (S110) Provide a sequencing analysis substrate with a replication library of oligos on a solid support; (S120) Sequence the replication library; (S130) Obtain the actual location information of the solid support; (S140) Map pixel information and the actual location information; (S150) Extract the solid support using a mapping result; (S160) Massively replicate by amplifying the oligos of the extracted solid support
Abstract translation: 目的：提供用于产生大量高纯度核苷酸的方法，以预定的碱基序列快速且准确地分离出微珠并以可用量进行放大。 构成：用于生产大量高纯度核苷酸的方法包括：在固体支持物上提供具有寡核苷酸复制文库的测序底物的步骤（S110）; 对复制库进行排序的步骤（S120）; 获得测序基板上的固体支持物的测量位置信息的步骤（S130）; 映射从固体支持物生成的像素信息和测量位置信息的步骤（S140）; 使用映射结果从测序底物中分离具有所需碱基序列的固体支持物的步骤（S150）; 和扩增分离的固体支持物的寡核苷酸的步骤（S160）。 （附图标记）（AA）开始; （BB）结束; （S110）在固体支持物上提供具有寡核苷酸复制文库的测序分析底物; （S120）顺序复制库; （S130）获取实体支持的实际位置信息; （S140）映射像素信息和实际位置信息; （S150）使用映射结果提取固体支持; （S160）通过扩增提取的固体支持物的寡核苷酸大量复制
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公开(公告)号：KR1020210023948A
公开(公告)日：2021-03-04
申请号：KR1020210024831
申请日：2021-02-24
Applicant: 서울대학교산학협력단
IPC: C12Q1/6806 , C12N15/10
Abstract: 본발명은저비용고순도로염기서열을합성할수 있는 DNA 합성기술로서, 유전체/유전자합성분야에유용하게사용되어질수 있는고순도뉴클레오타이드획득방법및 장치에관한것이다.
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Patent Agency Ranking
公开(公告)号：KR101471246B1
公开(公告)日：2014-12-10
申请号：KR1020120080601
申请日：2012-07-24
Applicant: 서울대학교산학협력단
CPC classification number: C12P19/34 , B01J2219/00315 , B01J2219/00441 , B01J2219/00468 , B01J2219/005 , B01J2219/00596 , B01J2219/00648 , B01J2219/00691 , B01J2219/00693 , B01J2219/00722 , C40B20/02 , C40B50/14 , C40B60/04
Abstract: 고체지지체상에존재하는올리고뉴클레오타이드들의복제라이브러리를갖는염기서열분석기판을제공하는단계; 상기복제라이브러리를시퀀싱하는단계; 상기염기서열분석기판상의상기고체지지체의실측위치정보를얻는단계; 상기시퀀싱의결과로주어지는상기고체지지체로부터발생한신호의픽셀정보와상기실측위치정보를맵핑하는단계; 상기맵핑한결과를이용하여원하는염기서열을갖는상기고체지지체를상기염기서열분석기판으로부터추출하는단계; 및상기추출된상기고체지지체상의올리고뉴클레오타이드를증폭하여대량으로복제하는단계를포함하는고순도뉴클레오타이드의대량생산방법이제공된다.
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公开(公告)号：KR1020130046356A
公开(公告)日：2013-05-07
申请号：KR1020120114103
申请日：2012-10-15
Applicant: 서울대학교산학협력단
CPC classification number: C12P19/34 , B01J2219/00315 , B01J2219/00441 , B01J2219/00468 , B01J2219/005 , B01J2219/00596 , B01J2219/00648 , B01J2219/00691 , B01J2219/00693 , B01J2219/00722 , C40B20/02 , C40B50/14 , C40B60/04 , C12Q1/6811 , C12Q1/6834
Abstract: PURPOSE: A mass production method of a high purity nucleotide is provided to quickly and accurately isolate a microbead with a predetermined sequence and to amplify the sequence. CONSTITUTION: A mass production method of a high purity nucleotide comprises: a step of providing a sequencing substrate with a replication library of oligonucleotides existing on a solid support(S110); a step of sequencing the replication library(S120); a step of obtaining measured location information of the solid support(S130); a step of mapping pixel information of a signal generated from the solid support and the measured location information(S140); a step of extracting a desired sequence from the sequencing substrate using the mapping result(S150); and a step of amplifying a large amount of oligonucleotides on the solid support(S160). [Reference numerals] (AA) Start; (BB) End; (S110) Provide a sequencing analysis substrate with a replication library of oligos on a solid support; (S120) Sequence the replication library; (S130) Obtain the actual location information of the solid support; (S140) Map pixel information and the actual location information; (S150) Extract the solid support using a mapping result; (S160) Massively replicate by amplifying the oligos of the extracted solid support
Abstract translation: 目的：提供高纯度核苷酸的大规模生产方法，以预定的顺序快速准确地分离微珠并扩增序列。 构成：高纯度核苷酸的批量生产方法包括：向测序底物提供存在于固体支持物上的寡核苷酸复制文库的步骤（S110）; 对复制库进行排序的步骤（S120）; 获取固体支持物的测量位置信息的步骤（S130）; 映射从固体支持物产生的信号的像素信息和所测量的位置信息的步骤（S140）; 使用映射结果从测序底物提取期望的序列的步骤（S150）; 和在固体支持物上扩增大量寡核苷酸的步骤（S160）。 （附图标记）（AA）开始; （BB）结束; （S110）在固体支持物上提供具有寡核苷酸复制文库的测序分析底物; （S120）顺序复制库; （S130）获取实体支持的实际位置信息; （S140）映射像素信息和实际位置信息; （S150）使用映射结果提取固体支持; （S160）通过扩增提取的固体支持物的寡核苷酸大量复制
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公开(公告)号：KR1020170089649A
公开(公告)日：2017-08-04
申请号：KR1020160010129
申请日：2016-01-27
Applicant: 서울대학교산학협력단
Abstract: 시료의컨텐츠정보를제공하는시스템이개시된다. 본발명의일실시예의시스템은, 디스플레이부, 생물학적시료의영상에서위치정보가확인된대상시료에대응하는서열정보를포함하는컨텐츠정보를생성하는분석부, 생물학적시료의영상을생성하는영상생성부, 및상기생물학적시료의영상및 상기컨텐츠정보를디스플레이하도록상기디스플레이부를제어하는제어부를포함한다.
Abstract translation: 公开了一种用于提供样本的内容信息的系统。 本发明的一个实施例的显示单元中，样品以生成内容信息，以所述位置信息包括对应于所识别的目标试样分析单元的序列信息的图像中的生物的系统中，图像以生成生物样本发生器的图像 以及控制单元，用于控制显示单元显示生物样本的图像和内容信息。
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公开(公告)号：KR1020170051384A
公开(公告)日：2017-05-11
申请号：KR1020170049147
申请日：2017-04-17
Applicant: 서울대학교산학협력단
IPC: G01N1/28
Abstract: 시료가적층된기판을제공하는단계; 상기시료의층의영역들중 하나이상의원하는분리대상영역을선택하는단계; 상기분리대상영역에상당하도록에너지원을패턴화하는단계; 및상기패턴화된에너지원으로부터추출된에너지를상기분리대상영역에인가함으로써상기분리대상영역에해당하는목적시료를상기기판으로부터이탈시켜회수하는단계를포함하는기판으로부터시료를선택적으로분리하는방법이제공된다.
Abstract translation: 提供其上层压有样品的基板; 选择样品层的区域的一个或多个期望的分离目标区域; 构图能源以符合分离主题区域; 并且通过将从图案化的能量源提取的能量施加到分离对象区域，从基底分离和回收对应于分离对象区域的目标样本， 它提供。
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公开(公告)号：KR1020170047182A
公开(公告)日：2017-05-04
申请号：KR1020160137934
申请日：2016-10-21
Applicant: 서울대학교산학협력단
CPC classification number: C12Q1/68 , G01N33/53 , G03F7/20 , C12Q1/686 , C12Q1/6869 , C12Q2563/107 , C12Q2565/607 , C12Q2565/627 , G03F7/7005 , G03F7/70391
Abstract: 생체시료들이배치된기판을준비하는단계; 상기시료들중 적어도하나의목적시료가위치하는영역과적어도하나의비목적시료가위치하는영역을구분하는단계; 상기비목적시료가위치하는영역을선택적으로마스킹하기위한마스킹구조물을형성하는단계; 상기목적시료가위치하는영역에생화학반응시약을도입하여상기목적시료를반응시키는단계; 및상기목적시료를상기기판상에서분석하거나상기기판으로부터회수하여분석하는단계를포함하는생체시료의선별적분석방법이제공된다.
Abstract translation: 准备生物样品排列在其上的基底; 划分至少一个目标样本所在的区域和至少一个非目标样本所在的区域; 形成掩模结构，用于选择性地掩蔽非物体样品所在的区域; 将生化反应试剂引入到目标样品所在的区域以使目标样品反应; 分析底物上的目标样品或从底物中回收样品并分析样品。
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