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公开(公告)号：KR1020090110159A
公开(公告)日：2009-10-21
申请号：KR1020080035792
申请日：2008-04-17
Applicant: 성균관대학교산학협력단
Abstract: PURPOSE: A quantitative analysis method is provided to apply a small amount of protein in disease diagnosis sensor and proteomics research. CONSTITUTION: A quantitative protein analysis method comprises: a step of fixing PSA antibody to gold nano particle to produce gold nano probe; a step of fixing PSA antibody to a magnetic microbead and reacting the gold nano probe and magnetic microbead with PSA-ACT antigen in a solution; a step of separating the gold nano probe and magnetic microbead; a step of eluting the gold nano probe and magnetic microbead; a step of separating the magnetic microbead from the solution; a step of adding AA and CTAB to the gold nano probe; and a step of measuring the amount of gold nano probe.
Abstract translation: 目的：提供定量分析方法，在疾病诊断传感器和蛋白质组学研究中应用少量蛋白质。 构成：定量蛋白质分析方法包括：将PSA抗体固定在金纳米颗粒上以制备金纳米探针的步骤; 将PSA抗体固定在磁性微珠上并使金纳米探针和磁性微珠与PSA-ACT抗原反应在溶液中的步骤; 分离金纳米探针和磁性微珠的步骤; 洗脱金纳米探针和磁性微珠的步骤; 将磁性微珠与溶液分离的步骤; 向金纳米探针添加AA和CTAB的步骤; 以及测量金纳米探针量的步骤。
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公开(公告)号：KR100879206B1
公开(公告)日：2009-01-16
申请号：KR1020050133722
申请日：2005-12-29
Applicant: 성균관대학교산학협력단
CPC classification number: G01N33/569 , B82Y15/00 , B82Y30/00 , G01N33/54373 , G01N33/554 , Y10S436/805
Abstract: 본 발명은 i) 병원성 미생물과 이에 대한 항체를 회분식으로 면역 반응시키는 단계; ii) 상기 항체와 결합된 병원성 미생물을 선택적 분리하는 단계; 및 iii) 상기 항체와 결합된 병원성 미생물을 흐름식 표면 플라즈몬 공명(SPR)시스템 칩 상에 실시간으로 결합시키는 단계를 포함하는, 변형 흐름식 SPR 바이오센서를 이용하는 병원성 미생물의 실시간 검출 방법에 관한 것이다.
흐름식 SPR시스템 칩, 회분식 면역 반응, 바이오틴-스트렙트아비딘 결합-
公开(公告)号：KR100663713B1
公开(公告)日：2007-01-03
申请号：KR1020050124361
申请日：2005-12-16
Applicant: 성균관대학교산학협력단
IPC: C08F38/02
CPC classification number: G01N33/54353 , G01N33/5432
Abstract: Provided is a novel color shift sensor comprising a polydiacetylene supermolecule, which fixes a thiol group-containing receptor like an antibody, shows a color shift sensor upon the reaction with a sample, and is useful for detection of an antigen when used in an antibody-antigen reaction. The color shift sensor comprises a polyacetylene supermolecule capable of fixing a thiol group-containing receptor molecule. The polyacetylene supermolecule is obtained by mixing a diacetylene monomer represented by the following formula 1, which has a terminal -COOH group non-reactive to thiol, with a diacetylene monomer represented by the following formula 2, which has a lipid molecule capable of reacting with thiol. In the formulae 1 and 2, R1 is a C1-C10 alkyl; R2 is a C1-C10 alkyl; R3 is the formula (a) (wherein n is an integer of 1-10); and R4 is the formula (b).
Abstract translation: 提供了一种新颖的色移传感器，其包含固定含硫醇基的受体（如抗体）的聚二乙炔超分子，在与样品反应时显示出色移传感器，并且当用于抗体时可用于检测抗原， 抗原反应。 色移传感器包括能够固定含硫醇基的受体分子的聚乙炔超分子。 聚乙炔超分子通过将具有与硫醇无反应性的末端-COOH基团的由下式1表示的二乙炔单体与由下式2表示的二乙炔单体混合而获得，该乙炔单体具有能够与 硫醇。 在式1和式2中，R 1是C 1 -C 10烷基; R2是C1-C10烷基; R3是式（a）（其中n是1-10的整数）; R4为式（b）。
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公开(公告)号：KR1020110090565A
公开(公告)日：2011-08-10
申请号：KR1020100010421
申请日：2010-02-04
Applicant: 성균관대학교산학협력단
Abstract: PURPOSE: A binary vector is provided to stably express foreign gene induced in microalgae and to easily select transformed microalgae. CONSTITUTION: A reporter gene for sorting transformed microalgae has optimized 3rd codon in a reporter gene. The GC% of 3rd codon in the reporter gene is 70-90%. The reporter gene is crGUS, crGFP, or crlux. crGUS is denoted by sequence number 1. A binary vector contains the reporter gene. The vector additionally contains pSaD promoter, rbcS2 promoter, pHsp70A promoter, or beta-tublin promoter, and a target gene which is operatively linked to the promoter. The promoter is derived from microalgae. The promoter contains a microalgae-derived intron at the downstream. The target gene is bleomycin resistance gene(ble) or paromomycin resistance gene(part).
Abstract translation: 目的：提供二元载体，以稳定表达在微藻中诱导的外源基因，并易于选择转化的微藻。 构成：用于分选转化的微藻的报告基因已经在报道基因中优化了第3个密码子。 报道基因中第3个密码子的GC％为70-90％。 报告基因是crGUS，crGFP或cr。。 crGUS由序列号1表示。二元载体含有报告基因。 该载体另外含有pSaD启动子，rbcS2启动子，pHsp70A启动子或β-都柏林启动子，以及与启动子有效连接的靶基因。 启动子来自微藻。 启动子在下游含有微藻衍生的内含子。 目标基因为博莱霉素抗性基因（ble）或巴龙霉素抗性基因（部分）。
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公开(公告)号：KR100996532B1
公开(公告)日：2010-11-24
申请号：KR1020080082507
申请日：2008-08-22
Applicant: 성균관대학교산학협력단 , 주식회사 엠아이텍
CPC classification number: G01N33/54353 , B82Y5/00 , B82Y30/00 , B82Y40/00 , C01B32/174 , G01N33/54373 , G01N33/54393
Abstract: 본 발명은 탄소나노튜브 기반 바이오센서에서 링커와 스페이서를 적절한 비율로 혼합한 후, 탄소나노튜브 표면에 고정화하여 표적 물질을 극소량까지 검출하는 방법에 관한 것이다. 이러한 방법은 특정 물질을 펨토몰 수준까지 검출할 수 있는 방법으로써 기존의 탄소나노튜브 트랜지스터 센서의 검출 한계를 훨씬 더 낮출 수 있으므로 극미량의 표적 물질도 검출 가능하여 의료용 질병 진단 센서나 환경 센서로의 이용이 가능한 획기적인 분석 방법으로 이용할 수 있다.
바이오센서, 탄소나노튜브, 링커, 스페이서, 민감도-
Patent Agency Ranking
公开(公告)号：KR1020100119018A
公开(公告)日：2010-11-09
申请号：KR1020090037915
申请日：2009-04-30
Applicant: 삼성전자주식회사 , 성균관대학교산학협력단
CPC classification number: C12P7/10 , C08H8/00 , C12P2201/00 , C13K1/02 , Y02E50/16 , Y02E50/343 , Y02P30/20 , C08B1/08 , C10G3/00 , C12P7/04 , C12R1/865
Abstract: PURPOSE: A pretreatment method of a lignocellulose-based biomass is provided to obtain cellulose with high purity and hemicelluloses with the high yield, by extracting lignin first. CONSTITUTION: A pretreatment method of a lignocellulose-based biomass comprises the following steps: adding a solvent dissolving lignin to the lignocellulose-based biomass containing the lignin, hemicelluloses, and cellulose, to extract the lignin from the biomass(S1,S2); and adding an ionic solution to the biomass without the lignin, to extract the hemicelluloses and the cellulose(S3). The solvent dissolving the lignin is an alkaline solvent.
Abstract translation: 目的：提供木质纤维素基生物质的预处理方法，首先通过提取木质素，以高产率获得高纯度的纤维素和半纤维素。 构成：木质纤维素基生物质的预处理方法包括以下步骤：向含木质素，半纤维素和纤维素的木质纤维素基生物质中加入溶解木质素的溶剂，从生物质提取木质素（S1，S2）; 并将离子溶液加入到没有木质素的生物质中，以提取半纤维素和纤维素（S3）。 溶解木质素的溶剂是碱性溶剂。
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公开(公告)号：KR100962290B1
公开(公告)日：2010-06-11
申请号：KR1020080012921
申请日：2008-02-13
Applicant: 성균관대학교산학협력단
CPC classification number: G01N33/54346 , G01N33/54373
Abstract: 금 나노입자의 국지화 된 표면 플라즈몬 공명 (Localized Surface Plasmon Resonance, LSPR) 현상을 이용한 생체물질 검출법에 관한 것으로서, 제조된 금 나노입자를 유리판에 고정화시킨 후 항원-항체 반응을 통해 최대파장 변화를 이용하여 생체물질을 진단할 수 있는 방법이다. 이러한 방법을 이용한 센서는 민감도가 높고, 값싸고, 빠른 시간에 진단을 할 수 있어 병 진단뿐만 아니라 환경 오염물질, 병원균 등 다양한 생물학적 분야에 응용 가능케 하며, 기존의 SPR을 이용한 방법보다 싸고, 빠르고, 더 높은 민감도의 센서를 제작할 수 있는 기술이다.
금, 나노입자, LSPR, SPR, 생체물질, 개질, 항원항체반응, 센서-
公开(公告)号：KR1020090087594A
公开(公告)日：2009-08-18
申请号：KR1020080012921
申请日：2008-02-13
Applicant: 성균관대학교산학협력단
CPC classification number: G01N33/54346 , G01N33/54373 , G01N21/47 , G01N33/553
Abstract: A method for detecting a bio material using a localized surface Plasmon resonance (LSPR) of gold nanoparticle is provided to diagnose at short time with high sensitivity and detect environmental pollutant and pathogen. A method for detecting a bio material comprises: a step of fixing a receptor in a localized surface Plasmon resonance sensor for detecting bio material; a step of letting a target material spill; and a step of measuring light scattering spectrum and analyzing mobility of maximum wave length using resonant Rayleigh scattering microspectroscopy and dark-field microscopy.
Abstract translation: 使用局部表面检测生物材料的方法提供金纳米颗粒的等离子体共振（LSPR），以高灵敏度在短时间内诊断，并检测环境污染物和病原体。 用于检测生物材料的方法包括：将受体固定在局部表面的等离子体共振传感器中用于检测生物材料的步骤; 使目标物料溢出的一个步骤; 以及使用共振瑞利散射显微光谱和暗视野显微镜测量光散射光谱和分析最大波长的迁移率的步骤。
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公开(公告)号：KR1020080093529A
公开(公告)日：2008-10-22
申请号：KR1020070037358
申请日：2007-04-17
Applicant: 성균관대학교산학협력단 , 주식회사 엠아이텍
IPC: G01N27/327 , G01N33/487 , C12Q1/68 , B82B1/00
CPC classification number: G01N27/327 , B82B1/00 , C12Q1/6825
Abstract: A transistor based-biosensor for detecting minute target materials is provided to maximize the sensitivity by effectively reducing the size of a bio receptor using Fab reacting to antigen. A transistor based-biosensor for detecting minute target materials comprises a conductive material constituting a substrate, a source electrode, a drain electrode and a gate electrode, and a channel area. The gate electrode is composed of biomolecules. The conductive material has a bio receptor with a size below 5nm selected from a group consisting of oilgopeptide, oligonucleotide, ligand, Fab', F(ab')2, Fab, Fv, sFv, DsFv, and dAb.
Abstract translation: 提供了用于检测微小靶物质的基于晶体管的生物传感器，以通过使用Fab与抗原反应来有效减小生物受体的大小来使灵敏度最大化。 用于检测微小目标材料的基于晶体管的生物传感器包括构成基板的导电材料，源电极，漏电极和栅电极以及沟道区。 栅电极由生物分子组成。 导电材料具有选自油肽，寡核苷酸，配体，Fab'，F（ab'）2，Fab，Fv，sFv，DsFv和dAb的尺寸低于5nm的生物受体。
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20.发明授权
公开(公告)号：KR100775334B1
公开(公告)日：2007-11-09
申请号：KR1020060109825
申请日：2006-11-08
Applicant: 성균관대학교산학협력단
IPC: G01N33/569 , G01N33/53
Abstract: A method for real time detection of pathogenic bacteria by using a competitive immunoreaction-mediated flow type surface plasmon resonance(SPR) biosensor is provided to improve ability for detecting low concentration of waterborne pathogenic bacteria including Cryptosporidium parvum without labeling. A method for real time detection of pathogenic bacteria comprises the steps of: (i) immunoreacting a pathogenic bacterium with antibodies thereof(first antibody) in a batch type; (ii) selectively separating unreacted antibodies by using cut-off membrane filter; (iii) binding the separated unreacted antibodies to a second antibody immobilized to the surface of a flow type surface plasmon resonance(SPR) system chip in real time; and (iv) continuously collecting the real time SPR signal generated from the surface of flow type SPR system chip of unreacted antibody.
Abstract translation: 提供了一种通过使用竞争性免疫反应介导的流式表面等离子体共振（SPR）生物传感器实时检测病原菌的方法，以提高检测低浓度水分病原菌（包括无标记隐孢子虫）的能力。 用于实时检测病原菌的方法包括以下步骤：（i）以批次类型的抗体（第一抗体）免疫反应致病细菌; （ii）通过使用截止膜过滤器选择性分离未反应的抗体; （iii）将分离的未反应抗体与固定在流式表面等离子共振（SPR）系统芯片表面上的第二抗体实时结合; 和（iv）连续收集从未反应抗体的流式SPR系统芯片的表面产生的实时SPR信号。
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