公开(公告)号：KR1020140086234A

公开(公告)日：2014-07-08

申请号：KR1020120156471

申请日：2012-12-28

Applicant: 성균관대학교산학협력단

Inventor： 이석찬 ,  변희성 ,  길의준 ,  조상호 ,  이민지 ,  박정안 ,  김재덕 ,  최홍수 ,  김미경 ,  곽해련

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q2527/101 ,  C12Q2563/107 ,  C12Q2565/125

Abstract: The present invention relates to a set of four primers for isothermal amplification reaction for detecting Cucumber mosaic virus, a composition including the same, and a method for detecting the Cucumber mosaic virus using the composition. By using the primer set, all five mutants of the Cucumber mosaic virus can be effectively detected without a professional device in a short time using the isothermal amplification method. In addition, the Cucumber mosaic virus can be rapidly diagnosed with a naked eye under natural light using highly concentrated SYBR Green I. Therefore, the present invention facilitates early detection of virus which harms farms cultivating crops such as cucumbers, water melons, hot peppers, and the like, thereby making it possible to rapidly and efficiently construct a system for diagnosing the Cucumber mosaic virus.

Abstract translation: 本发明涉及用于检测黄瓜花叶病毒的等温扩增反应的四个引物，包含其的组合物以及使用该组合物检测黄瓜花叶病毒的方法。 通过使用引物组，使用等温扩增方法可以在短时间内无需专业设备有效地检测黄瓜花叶病毒的所有5种突变体。 此外，使用高度浓缩的SYBR Green I，可以在自然光下用肉眼快速诊断黄瓜花叶病毒。因此，本发明有助于早期检测危害农作物的培养，例如黄瓜，西瓜，辣椒， 从而能够快速有效地构建用于诊断黄瓜花叶病毒的系统。