公开(公告)号：KR100819337B1

公开(公告)日：2008-04-02

申请号：KR1020060053250

申请日：2006-06-14

Applicant: 재단법인서울대학교산학협력재단 ,  삼성전자주식회사

Inventor： 이성훈

IPC: H01L33/00 ,  B82Y20/00

Abstract: 본 발명은 단파장 발광원과 발광원으로부터 나오는 빛을 흡수하여 파장 변환된 빛을 방출하는 반도체 양자점(quantum dot) 나노 형광체를 결합하여 백색광을 발현하는, 단파장광원과 양자점 나노형광체로 구성되는 소자 구조의 백색광다이오드(White LED)를 제조하는 기술이다.

公开(公告)号：KR100771171B1

公开(公告)日：2007-10-29

申请号：KR1020060062807

申请日：2006-07-05

Applicant: 재단법인서울대학교산학협력재단 ,  (주)모디셀

Inventor： 강경선 ,  이성훈 ,  손용진 ,  정미경

IPC: C12N5/071 ,  A61K35/36

CPC classification number: C12N5/0628 ,  A61K35/12 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/599 ,  C12N2501/70 ,  A61K35/36 ,  C12N2500/99

Abstract: A method for preparing hair follicle stem cells is provided to use the obtained stem cells capable of being differentiated into hair follicle cells and growing hair as a new cell therapeutic agent of hair loss. A method for isolating hair follicle stem cells and differentiating them into hair follicle cells comprises the steps of: (a) after finely incising scalp tissue including hair follicle, performing a first chemical decomposition of the tissue in a culture medium comprising a protease, a collagenolytic enzyme and a DNAse enzyme; (b) performing a second chemical decomposition on the tissue obtained from the step(a) in a culture medium comprising collagenase; (c) after recovering the chemically decomposed tissue and culturing scalp cells in a culture medium including 1-30% of serum, culturing it in a non-serum culture medium after cell attachment; (d) after recovering the cultured scalp cells, isolating CD34+ therefrom and isolating hair follicle stem cells; and (e) hypodermically injecting the isolated hair follicle stem cells into the scalp tissue to be differentiated into hair follicle cells, wherein the culture medium for the first chemical decomposition comprises 1-10% of an enzyme complex consisting of 0.1-2mg/ml of dispase, protease, collagenolytic enzyme and DNAse enzyme in a DMEM culture medium including 1-30% of fetal bovine serum, 10-200mug/ml of normocin, and 1-10% of penicillin-streptomycin and the culture medium for the second chemical decomposition comprises 0.1-10mg/ml of collagenase type I A in the DMEM culture medium. A composition for promoting hair growth comprises the hair follicle stem cells as an effective ingredient.

Abstract translation: 提供了一种用于制备毛囊干细胞的方法，以使得能够分化为毛囊细胞并且生长毛发的获得的干细胞作为脱发的新的细胞治疗剂。 一种用于分离毛囊干细胞并将其分化成毛囊细胞的方法包括以下步骤：（a）在精细切开包括毛囊的头皮组织之后，在包含蛋白酶，胶原分解的培养基中进行组织的第一次化学分解 酶和DNA酶; （b）在包含胶原酶的培养基中对由步骤（a）获得的组织进行第二次化学分解; （c）在回收化学分解组织并培养包含1-30％血清的培养基中培养头皮细胞后，在细胞附着后在非血清培养基中培养; （d）回收培养的头皮细胞后，从其中分离出CD34 +并分离毛囊干细胞; 和（e）将分离的毛囊干细胞皮下注射到头皮组织中以分化成毛囊细胞，其中用于第一化学分解的培养基包含1-10％的由0.1-2mg / ml 分泌酶，蛋白酶，胶原分解酶和DNA酶在含有1-30％的胎牛血清，10-200ugug / ml的normocin和1-10％的青霉素 - 链霉素的DMEM培养基中，以及用于第二次化学分解的培养基 在DMEM培养基中含有0.1-10mg / ml的IA型胶原酶。 用于促进毛发生长的组合物包括作为有效成分的毛囊干细胞。