-
公开(公告)号：KR1020070013432A
公开(公告)日：2007-01-31
申请号：KR1020050067736
申请日：2005-07-26
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N5/16
CPC classification number: C12N15/877
Abstract: Cloned Canidae and a method for producing thereof are provided to clone the Canidae by somatic cell nuclear transfer, so that the method is useful for propagation of excellent Canidae species and preservation of rare and endangered Canidae species. The method for producing the cloned Canidae comprises the steps of: (a) removing a nucleus from the matured oocyte of Canidae; (b) separating the somatic cell from the tissue of Canidae so as to prepare a nucleus donor cell; (c) micro-injecting the nucleus donor cell into the denucleated oocyte, and electronically fusing the oocyte by applying voltage of 3.0-3.5 kv/cm to the oocyte; and (d) activating the electrofused oocyte by treating the electrofused oocyte with calcium ionophore and DMAP(6-dimethylaminopurine), wherein the somatic cell is fibroblast or cumulus cell; the Canidae is dog, wolf, fox, jackal, coyote, Korean wolf or raccoon. The cloned Canidae oocyte is deposited under KCTC 10831BP.
Abstract translation: 提供了克隆犬科及其生产方法，通过体细胞核转移克隆犬科，使该方法对于优良犬科的种繁殖和保存珍稀濒危的犬科物种有用。 产生克隆的犬科的方法包括以下步骤：（a）从犬科的成熟卵母细胞中除去核; （b）从犬科的组织分离体细胞以制备核供体细胞; （c）将核供体细胞微注射到去核卵母细胞中，并通过向卵母细胞施加3.0-3.5kv / cm的电压来电子融合卵母细胞; 和（d）通过用钙离子载体和DMAP（6-二甲氨基嘌呤）处理电熔卵母细胞来激活电熔卵母细胞，其中体细胞是成纤维细胞或卵丘细胞; 犬科是狗，狼，狐狸，豺狼，土狼，韩国狼或浣熊。 克隆的犬科卵母细胞保藏在KCTC 10831BP下。
-
公开(公告)号：KR1020040045528A
公开(公告)日：2004-06-02
申请号：KR1020020073333
申请日：2002-11-23
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N5/16
CPC classification number: C12N15/8771 , A01K67/0273 , A01K2227/101 , A01K2267/01 , A61D19/04 , C12N15/8509 , C12N2015/8518 , C12N2510/00 , C12N2510/02 , C12N2517/04
Abstract: PURPOSE: Transgenic cloned cow producing human alpha1-antitrypsin and a method for producing the same are provided, thereby economically and efficiently producing biological medicines from the cloned animals. CONSTITUTION: The method for producing the transgenic cloned cow producing human alpha1-antitrypsin comprises the steps of: (a) preparing nucleus donor cells by transferring a gene encoding human alpha1-antitrypsin to somatic cell lines collected from cow; (b) preparing matured nucleus recipient ova collected from cow by removing oocytes from nucleus recipient ova of cow and removing cytoplasm including the first polar body; and (c) transferring the nucleus donor cells to the matured nucleus recipient ova and fusing them, wherein the somatic cells are collected from matured cow; and the nucleus transplanted ovum is SNU-B3(KCTC 10356BP).
Abstract translation: 目的：提供转基因克隆牛产生人α1-抗胰蛋白酶及其制备方法，从而经济有效地从克隆的动物产生生物药物。 构成：生产人α1-抗胰蛋白酶的转基因克隆牛的方法包括以下步骤：（a）通过将编码人α1-抗胰蛋白酶的基因转移到从母牛收集的体细胞系中来制备核供体细胞; （b）通过从牛的核受体卵中除去卵母细胞并除去包括第一极体的细胞质来制备从牛采集的成熟的细胞核受体ova; 和（c）将核供体细胞转移到成熟的细胞核受体ova并使其融合，其中从成熟的牛收集体细胞; 移植的卵细胞为SNU-B3（KCTC 10356BP）。
-
Patent Agency Ranking
公开(公告)号：KR100975350B1
公开(公告)日：2010-08-12
申请号：KR1020020075471
申请日：2002-11-29
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N5/16
Abstract: 본 발명은 사람 락토페리신을 발현하는 유전자를 성숙한(adult) 소에서 유래한 체세포에 도입 및 적중시키는 단계; 상기의 사람 락토페리신을 발현하는 외래 유전자가 도입 및 적중된 체세포를 이용하여 복제소를 생산하는 단계를 포함하는 형질전환 복제소의 생산방법 및 이러한 방법에 의해 생산된 락토페리신을 발현하는 형질전환(transgenic) 복제소에 관한 것이다.
또한, 본 발명은 사람 락토페리신을 발현하는 유전자를 성숙한 소의 체세포에 도입 및 적중시키고, 상기 형질전환된 체세포를 이용하여 생산된 복제소의 유선으로부터 사람 락토페리신을 생산하는 방법에 관한 것이다.
사람 알파1-안티트립신, 체세포 복제, 형질전환 복제 소-
公开(公告)号：KR1020090075637A
公开(公告)日：2009-07-08
申请号：KR1020090000331
申请日：2009-01-05
Applicant: 재단법인서울대학교산학협력재단
IPC: A01K67/027 , C12N15/09
CPC classification number: A01K67/0275 , A01K2227/10 , C07K14/43595 , C12N15/873
Abstract: A method of manufacturing a cloned dog is provided, which uses transfection and targeting technology of the desired gene. A method of manufacturing a cloned dog comprises: a step for producing the donor nucleus cell from the somatic cell line picked from the dog and expressing by introducing the DNA structure containing the target gene; a step for adding the cell cycle synchronization inducing agent and cultivating; a step for producing the nuclear transfer embryo by transplanting the cultivated donor nucleus cell to the denucleated dog recipient; a step for activating; and a step for producing a litter.
Abstract translation: 提供了制造克隆狗的方法，其使用所需基因的转染和靶向技术。 制造克隆狗的方法包括：从从狗取出的体细胞系中产生供体核细胞并通过引入含有靶基因的DNA结构进行表达的步骤; 添加细胞周期同步诱导剂并培养的步骤; 通过将培养的供体核细胞移植到去核的狗接受者来生产核转移胚的步骤; 激活的一个步骤 以及生产垃圾的步骤。
-
公开(公告)号：KR1020090051716A
公开(公告)日：2009-05-22
申请号：KR1020080115190
申请日：2008-11-19
Applicant: 재단법인서울대학교산학협력재단
CPC classification number: C12N5/16 , A01K67/0273 , A01K2227/10 , C12N15/877 , C12N2501/405 , C12N2517/10
Abstract: 본 발명은 개과 (
Canidae ) 동물의 체세포 핵 이식 산자 생산 효율을 향상시키는 방법에 관한 것이다. 보다 구체적으로 본 발명은 개의 난자로부터 핵을 제거하여 탈핵 난자를 제조하고 공여핵 세포를 상기 탈핵 난자에 융합시킴으로써 핵 이식란을 제조하여 이를 대리모의 난관에 이식함에 있어서, 상기 공여 핵 세포 배양시에 특정 세포 주기 동기화 유도물질을 첨가함으로써 복제 개 생산 효율을 향상시키는 방법에 관한 것이다. 본 발명에 따른 방법은 개과 동물을 고효율로 복제할 수 있게 함으로써 우량 품종의 번식, 이종이식, 질환 모델동물 등 수의학, 인류학 및 의학연구 분야의 발달에 기여할 수 있다.
복제개, 핵 이식, 세포 주기 동기화, 로스코비틴(Roscovitine)-
公开(公告)号：KR100829426B1
公开(公告)日：2008-05-15
申请号：KR1020070005350
申请日：2007-01-17
Applicant: 재단법인서울대학교산학협력재단
IPC: A01K67/00 , A01K67/027 , C12N5/07
CPC classification number: C12N15/873 , A01K67/027 , A01K2227/00 , A61D19/04
Abstract: A method for producing a cloned dog is provided to obtain a method for producing a cloned dog that produces an embryo in an optimal fusion condition. A preparing method of an embryo of a dog includes the steps of: preparing an enucleated ovum of an animal except for a human being; preparing a donor somatic cell; injecting minutely and electrically fusing the donor somatic cell; and activating the fused ovum. The electric fusion is conducted in a condition that the voltage is 3.8-5.0kV/cm. The electric fusion is conducted twice for 15-25mus by using a needle type electrode. The embryo is transplanted in an oviduct of a surrogate mother.
Abstract translation: 提供了克隆狗的制备方法，以获得在最佳融合条件下产生胚胎的克隆狗的制备方法。 狗的胚胎的制备方法包括以下步骤：制备除人之外的动物的去核卵; 准备供体体细胞; 注射和电熔融供体体细胞; 并激活融合的卵子。 电压在3.8-5.0kV / cm的电压下进行。 通过使用针式电极将电融合进行两次15-25m。 将胚胎移植到代孕母亲的输卵管中。
-
公开(公告)号：KR1020040101793A
公开(公告)日：2004-12-03
申请号：KR1020030033582
申请日：2003-05-27
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N5/16
CPC classification number: C12N15/8771 , A01K67/0273 , A01K2227/101 , A01K2267/01 , A61D19/04 , C12N15/8509 , C12N2015/8518 , C12N2517/00
Abstract: PURPOSE: Transgenic cloned cow producing human erythropoietin and a method for producing the same transgenic cloned cow are provided, which method allows economic and effective production of various biomedicines, and facilitates production of human erythropoietin in cow's milk. CONSTITUTION: The nuclear transfer embryo produced by a fusion of a cow-derived enucleated oocyte with a nucleus of a cow-derived somatic cell transfected with a gene encoding human erythropoietin is provided, wherein the cow is an adult; the nuclear transfer embryo is SNU-B4(KCTC 10450BP). The method for producing a transgenic cloned cow expressing human erythropoietin in the mammary gland comprises the steps of: (a) introducing a gene encoding human erythropoietin into a cow-derived somatic cell line to prepare a nuclear donor cell; (b) removing cumulus cells surrounding a recipient oocyte and then the cytoplasm including the first polar body from the oocyte to prepare a cow-derived enucleated recipient oocyte; (c) transferring the transfected nuclear donor cell into the enucleated recipient oocyte and carrying out cell fusion to generate nuclear transfer embryos; and (d) transplanting the nuclear transfer embryo into a surrogate mother cow to produce live offspring.
Abstract translation: 目的：提供转基因克隆的牛产生人促红细胞生成素和生产相同转基因克隆牛的方法，该方法允许经济有效地生产各种生物医学，并促进牛奶中人促红细胞生成素的产生。 构成：提供由牛衍生的去核卵母细胞与用编码人促红细胞生成素的基因转染的牛衍生的体细胞的细胞核融合而产生的核转移胚，其中，牛是成年人; 核转移胚是SNU-B4（KCTC 10450BP）。 用于产生在乳腺中表达人促红细胞生成素的转基因克隆牛的方法包括以下步骤：（a）将编码人促红细胞生成素的基因导入牛衍生的体细胞系以制备核供体细胞; （b）去除卵母细胞周围的卵丘细胞，然后从卵母细胞中除去包含第一极体的细胞质，以制备牛衍生的去核受体卵母细胞; （c）将转染的核供体细胞转移到去核的受体卵母细胞中并进行细胞融合以产生核转移胚胎; 和（d）将核转移胚胎移植到替代母牛中以产生活的后代。
-
-
-
-
-
-
Search Results
17
records
(took 0.046 seconds)
