公开(公告)号：KR100647793B1

公开(公告)日：2006-11-23

申请号：KR1020060071233

申请日：2006-07-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 유승관 ,  안지훈 ,  이종섭 ,  최양도

IPC: C12N15/29 ,  C12N15/10 ,  C12N15/09

Abstract: A method for controlling flowering time of plant by using ECL1 gene is provided to induce the early or late flowering of plants, and increase production yield of flowers and seeds or production amount of leaf and stem. The flowering time of plant is regulated by regulating the expression of a polynucleotide encoding ECL1 protein having the amino acid sequence of SEQ ID NO:2, wherein the plant is Arabidopsis thaliana; the polynucleotide encoding the ECL1 protein has the nucleotide sequence of SEQ ID NO:1; the flowering time of Arabidopsis thaliana is promoted by overexpression of ECL1 gene in Arabidopsis thaliana by introducing a recombinant vector containing the polynucleotide encoding the ECL1 protein into Arabidopsis thaliana; the flowering time of Arabidopsis thaliana is delayed by deletion of ECL1 gene or combining the antisense molecule to the polynucleotide encoding the ECL1 protein in Arabidopsis thaliana; and the introduction of recombinant vector into Arabidopsis thaliana is accomplished by Agrobacterium sp.-mediated method, particle gun bombardment, silicon carbide whiskers, sonication, electroporation or PEG(polyethylene glycol) precipitation.

Abstract translation: 提供一种利用ECL1基因控制植物开花时间的方法，诱导植物早，晚花，提高花，种子产量或叶，茎产量。 通过调节编码具有SEQ ID NO：2的氨基酸序列的ECL1蛋白的多核苷酸的表达来调节植物的开花时间，其中所述植物是拟南芥; 编码ECL1蛋白质的多核苷酸具有SEQ ID NO：1的核苷酸序列; 通过将编码ECL1蛋白的多核苷酸的重组载体导入拟南芥中，通过在拟南芥中过表达ECL1基因来促进拟南芥的开花时间; 拟南芥的开花时间由于ECL1基因的缺失或反义分子与拟南芥中编码ECL1蛋白的多核苷酸的结合而延迟; 并且通过农杆菌介导的方法，粒子枪轰击，碳化硅晶须，超声处理，电穿孔或PEG（聚乙二醇）沉淀将重组载体引入拟南芥中。

公开(公告)号：KR1020040084186A

公开(公告)日：2004-10-06

申请号：KR1020030019069

申请日：2003-03-27

Applicant: 재단법인서울대학교산학협력재단

Inventor： 이종섭 ,  이동근 ,  안지훈 ,  송상기 ,  최양도

IPC: C07K14/415

CPC classification number: C07K14/415 ,  C12N15/8261 ,  C12N15/8271 ,  Y02A40/146

Abstract: PURPOSE: A gene controlling the root development of a plant and a method for manipulating the root development of a plant using the same gene are provided, thereby improving the phenotype of a plant related to root development, and facilitating the growth of it. CONSTITUTION: The gene GmEXP1 having the nucleotide sequence of SEQ ID NO:1 controls the root development of a plant, and encodes a protein controlling the root development of a plant, wherein the protein has the amino acid sequence of SEQ ID NO:2 and is isolated from Glycine max. A recombinant vector pGA643/GmEXP1 contains the gene GmEXP1. A transformed bacterium produced by transforming a bacterium with the recombinant vector pGA643/GmEXP1. The method for facilitating the root development of a plant comprises inserting the gene GmEXP1 into the expression vector, and introducing the expression vector into the plant.

Abstract translation: 目的：提供控制植物根系发育的基因和使用相同基因操作植物根系发育的方法，从而改善与根发育相关的植物的表型，并促进其生长。 构成：具有SEQ ID NO：1的核苷酸序列的基因GmEXP1控制植物的根部发育，并编码控制植物根系发育的蛋白质，其中该蛋白质具有SEQ ID NO：2的氨基酸序列和 与最大甘氨酸分离。 重组载体pGA643 / GmEXP1含有GmEXP1基因。 通过用重组载体pGA643 / GmEXP1转化细菌产生的转化细菌。 用于促进植物根系发育的方法包括将基因GmEXP1插入表达载体中，并将​​表达载体引入植物中。