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1.发明授权
公开(公告)号：KR100792169B1
公开(公告)日：2008-01-07
申请号：KR1020060006407
申请日：2006-01-20
Applicant: 재단법인서울대학교산학협력재단
Abstract: 본 발명은 애기장대에서 분리한 전사인자 AtMYB44의 유전자를 포함하는 재조합 DNA를 전이함으로써 제조된 형질전환 식물체에 관한 것이다. 상기 전사인자 단백질은 식물호르몬 앱사이식산(abscisic acid; ABA)에 의하여 활성화 되어, 식물의 생장 발달과정을 조절하거나 저수분, 고염, 저온 등 외부 환경스트레스에 대하여 저항성을 부여하는 유전자들의 발현을 유도한다. 그러므로 상기 신규 유전자를 식물체에 도입하여 과다 발현시킴으로써 개화지연 및 광엽화 등 식물의 발달과정이 조절되거나 가뭄, 염해, 냉해 등 각종 환경스트레스에 대한 저항성을 나타내는 형질전환 식물체를 얻을 수 있다.
전사인자, 앱사이식산, 식물 생장조절, 광엽화, 개화지연, 환경스트레스 저항성 식물-
公开(公告)号：KR100510055B1
公开(公告)日：2005-08-25
申请号：KR1020030019069
申请日：2003-03-27
Applicant: 재단법인서울대학교산학협력재단
IPC: C07K14/415
CPC classification number: C07K14/415 , C12N15/8261 , C12N15/8271 , Y02A40/146
Abstract: 본 발명은 식물의 뿌리 발달 조절 유전자 및 이를 이용한 식물의 뿌리 발달 촉진방법에 관한 것이다. 보다 구체적으로, 본 발명은 대두(
Glycine max )로부터 분리한 식물의 뿌리 발달 조절 유전자
GmEXP1, 상기 유전자에 의해 암호화되는 엑스판신(expansin) 단백질 및 상기 유전자를 식물체에서 과다 발현시킴으로써 식물의 뿌리 발달을 촉진하는 방법에 관한 것이다. 본 발명에서 분리된
GmEXP1 유전자 및 상기 유전자로부터 발현되는 엑스판신 단백질은 식물의 뿌리 발달과 관련된 형질의 개선 및 타 식물체에서 뿌리 발달 조절 유전자의 탐색 등에 유용하게 이용 될 수 있다. 또한, 본 발명에 따라 식물의 뿌리 발달을 촉진함으로써 식물체의 성장을 촉진시킬 수 있는 효과가 있다.-
公开(公告)号：KR100833473B1
公开(公告)日：2008-05-29
申请号：KR1020070012375
申请日：2007-02-06
Applicant: 재단법인서울대학교산학협력재단
CPC classification number: C12N15/827 , C07K14/415
Abstract: An SVP(short vegetative phase) gene for controlling the flowering time is provided to mediate the signal transfer process in response to changes of ambient temperature and control the phase transition period in the development step of flower, so that the gene is useful for promoting the flowering time to produce rapidly flowers and seeds, or delaying the flowering time to increase production of leaf and stem. An SVP gene for controlling the flowering time has the nucleotide sequence of SEQ ID NO:1 and inhibits the expression of FT(flowering locus T) sequence by binding to CArG motif of the FT sequence. A transgenic plant is prepared by transforming a plant with a recombinant vector containing the SVP gene. The flowering time of a plant is delayed by overexpressing the SVP gene in the plant, or promoted by inhibiting expression of SVP gene in the plant, wherein the plant is Arabidopsis thaliana.
Abstract translation: 提供用于控制开花时间的SVP（短营养期）基因，以响应环境温度的变化介导信号转移过程，并控制花的发育步骤中的相变期，使得该基因可用于促进 开花时间快速生花和种子，或延迟开花时间，增加叶和茎的产量。 用于控制开花时间的SVP基因具有SEQ ID NO：1的核苷酸序列，并通过与FT序列的CArG基序结合来抑制FT（开花基因座T）序列的表达。 通过用含有SVP基因的重组载体转化植物来制备转基因植物。 通过在植物中过表达SVP基因延迟植物的开花时间，或通过抑制植物中SVP基因的表达来促进植物的开花时间，其中植物是拟南芥。
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4.发明授权새로운 트레할로스 생합성효소 융합유전자와 이로부터발현된 내열성 융합효소단백질을 이용한 트레할로스 생산방법 失效一种新型TREHALOSE合成融合基因和利用从基因表达的可热熔融融合蛋白的TREHALOSE生产方法
公开(公告)号：KR100781062B1
公开(公告)日：2007-11-30
申请号：KR1020060120416
申请日：2006-12-01
Applicant: 재단법인서울대학교산학협력재단
Abstract: A trehalose synthesizing fusion protein is provided to synthesize and hydrolyze maltooligosyltrehalose and increase the production efficiency of trehalose due to excellent heat-resistance. A maltooligosyltrehalose synthesis hydrolase(MhMTSHase) protein derived from Metallosphaera hakonesis(JCM8857) includes an amino acid sequence of SEQ ID : NO. 2. An MhMTSH gene encodes the MhMTSHase protein and includes a sequence of SEQ ID : NO. 1. A method for producing the protein comprises a step of culturing E. coli transformed by a recombinant vector including the MhMTSH gene. A method for producing trehalose comprises a step of adding substrate such as a water-soluble starch or a liquid corn starch to the MhMTSHase protein and then reacting it at a temperature of 65-75 deg.C under the pH of 5.0-6.0.
Abstract translation: 提供海藻糖合成融合蛋白以合成和水解麦芽糖基海藻糖，并且由于优异的耐热性而提高海藻糖的生产效率。 衍生自金丝酵母属（JCM8857）的麦芽寡糖基海藻糖合成水解酶（MhMTSHase）蛋白包含SEQ ID NO： MhMTSH基因编码MhMTSHase蛋白，并包括SEQ ID NO： 1.一种生产蛋白质的方法包括培养由包含MhMTSH基因的重组载体转化的大肠杆菌的步骤。 海藻糖的制造方法包括向MhMTSHase蛋白质添加水溶性淀粉或液体玉米淀粉等底物，然后在5.0〜6.0的pH下，在65-75℃的温度下进行反应。
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5.发明授权환경스트레스에 의해 활성화되는 유도성 프로모터 및 상기프로모터를 이용하여 공변세포에 특이적으로 목적단백질을생산하는 형질전환 식물을 얻는 방법 失效由环境应激活化的可诱导促进剂和使用促进剂在植物保护细胞中特异性产生目标蛋白的转基因植物的产生方法
公开(公告)号：KR100781059B1
公开(公告)日：2007-11-30
申请号：KR1020060120463
申请日：2006-12-01
Applicant: 재단법인서울대학교산학협력재단
Abstract: A method for preparing a transformed plant using a P_AtMYB44 promoter derived from Arabidopsis thaliana is provided to obtain the plant specifically expressing a target protein in a guard cell under various environmental stresses such as moisture deficiency, high salinity, and low temperature without influencing on the ordinary growth characteristics of the plant by linking the promoter to a target gene and then transforming the plant using the same. An environmental stress inducible P_AtMYB44 promoter derived from Arabidopsis thaliana includes a sequence described in SEQ ID : NO.1, wherein the environmental stress is drought, salt damage or cold-weather damage. A recombinant plant expression vector includes the promoter and is prepared by linking a target gene encoding a target protein to downstream of the promoter. A method for producing a target protein in a plant comprises the steps of: (a) transforming a plant using the recombinant plant expression vector; and (b) applying at least one environmental stress selected from the group consisting of drought, salt damage and cold-weather damage to the transformed plant. A method for preparing a transformed plant capable of specifically expressing a target protein in a guard cell comprises a step of transforming the recombinant plant expression vector into a plant.
Abstract translation: 提供使用来自拟南芥的P_AtMYB44启动子制备转化植物的方法，以在各种环境胁迫如水分不足，高盐度和低温下在保护细胞中特异性表达靶蛋白的植物，而不影响普通 通过将启动子与靶基因连接，然后使用其转化植物，植物的生长特性。 源自拟南芥的环境胁迫诱导型P_AtMYB44启动子包括SEQ ID NO：1所述的序列，其中环境胁迫是干旱，盐损害或寒冷天气损害。 重组植物表达载体包括启动子，并通过将编码靶蛋白的靶基因与启动子的下游连接来制备。 制备植物中靶蛋白的方法包括以下步骤：（a）使用重组植物表达载体转化植物; 和（b）将至少一种选自干旱，盐损害和寒冷天气损害的环境胁迫应用于转化植物。 制备能够在保护细胞中特异性表达靶蛋白质的转化植物的方法包括将重组植物表达载体转化到植物中的步骤。
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Patent Agency Ranking6.发明授权
公开(公告)号：KR100781070B1
公开(公告)日：2007-11-30
申请号：KR1020060120433
申请日：2006-12-01
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N15/29
Abstract: A method for preparing a plant with increased disease-resistance is provided to obtain a plant having high resistance against pathogenic fungi by transforming a lectin gene AtMJI2 derived from Arabidopsis thaliana and expressed by chitin and jasmonate, thereby significantly decreasing the loss induced by the infection of blight and harmful insects. A method for increasing the disease resistance of a plant comprises a step of transforming a recombinant plant expression vector including a lectin gene AtMJI2 derived from Arabidopsis thaliana to a plant, wherein the vector is pCaAtMJ12 depicted in Fig. 4. An Arabidopsis thaliana prepared by the method is characterized in that it has the increased disease resistance.
Abstract translation: 提供了一种具有增加抗病性的植物的制备方法，通过转化来自拟南芥并由甲壳素和茉莉酮酸酯表达的凝集素基因AtMJI2，从而显着降低由感染引起的损失，从而获得具有高抗病原真菌的植物 枯萎和害虫。 提高植物抗病性的方法包括将包含来自拟南芥的凝集素基因AtMJI2的重组植物表达载体转化到植物中的步骤，其中载体是图1所示的pCaAtMJ12。 通过该方法制备的拟南芥的特征在于其具有增加的抗病性。
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7.发明公开
公开(公告)号：KR1020070076918A
公开(公告)日：2007-07-25
申请号：KR1020060006407
申请日：2006-01-20
Applicant: 재단법인서울대학교산학협력재단
Abstract: Provided is an AtMYB44 protein derived from Arabidopsis thaliana which is a transcription factor controlling the expression of other genes, is used for preparing a transformed plant which could effectively control the flowering time of the plant without influencing on general growth characteristics, and has high resistance on various stress such as low temperature, moisture deficiency and high salinity. The DNA coding a transcription factor AtMYB44 controlling the expression of genes derived from Arabidopsis thaliana and concerned with the stress-resistance and the development process has a nucleotide sequence described as SEQ ID : NO. 1 and an amino acid sequence described as SEQ ID : NO. 2. The binary vector pCaAtMYB44 for expressing plant genes is constructed by recombining the AtMYB44 genes with pBI111L. A transformed plant in which the AtMYB44 is over-expressed is prepared by transforming the AtMYB44 gene using a vector pCaAtMYB44.
Abstract translation: 提供了一种来自拟南芥属的AtMYB44蛋白，其是控制其他基因表达的转录因子，用于制备可以有效控制植物开花时间而不影响一般生长特性的转化植物，并具有高抗性 各种应力如低温，水分不足和盐度高。 编码转录因子AtMYB44的DNA，其控制来自拟南芥的基因的表达并涉及应激抗性和发育过程，具有如SEQ ID NO：1所示的核苷酸序列。 1和如SEQ ID NO：1所示的氨基酸序列。 用于表达植物基因的二元载体pCaAtMYB44通过将AtMYB44基因与pBI111L重组而构建。 通过使用载体pCaAtMYB44转化AtMYB44基因来制备AtMYB44过表达的转化植物。
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公开(公告)号：KR1020040075252A
公开(公告)日：2004-08-27
申请号：KR1020030010772
申请日：2003-02-20
Applicant: 재단법인서울대학교산학협력재단
IPC: C07K14/415
CPC classification number: C12N15/827 , C07K14/415
Abstract: PURPOSE: A gene controlling the flowering time of plants and a method for manipulating the flowering time of plants using the same gene are provided, which gene is useful for improving properties associated with the flowering time in plants and screening the flowering time-controlling gene in other plants. CONSTITUTION: The gene LOV1 controlling the flowering time of plants comprises the nucleotide sequence set forth in SEQ ID NO:1 and has the nucleotide sequence set forth in SEQ ID NO:3, wherein the gene controlling the flowering time of plants encodes a protein LOV1 controlling the flowering time of plants having the amino acid sequence set forth in SEQ ID NO:2 which is isolated from Arabidopsis thaliana; and the gene inhibits the flowering stimulating gene AGL20. The method for manipulating flowering time of plants comprises over-expressing the gene LOV1 for delaying the flowering time or inhibiting the expression of the gene LOV1 for stimulating the flowering time.
Abstract translation: 目的：提供控制植物开花时间的基因和使用相同基因操作植物开花时间的方法，该基因可用于改善与植物开花时间相关的性质，并筛选开花时间控制基因 其他植物。 构成：控制植物开花时间的基因LOV1包含SEQ ID NO：1所示的核苷酸序列，并具有SEQ ID NO：3所示的核苷酸序列，其中控制植物开花时间的基因编码蛋白LOV1 控制具有从拟南芥分离的SEQ ID NO：2所示的氨基酸序列的植物的开花时间; 并且该基因抑制开花刺激基因AGL20。 用于操作植物开花时间的方法包括过表达基因LOV1以延迟开花时间或抑制基因LOV1的表达以刺激开花时间。
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公开(公告)号：KR100648146B1
公开(公告)日：2006-11-29
申请号：KR1020060071234
申请日：2006-07-28
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N15/82
Abstract: A method for promoting the flowering time of plants by using AGL28 gene is provided to increase production yield of flowers and seeds by overexpressing the AGL28 gene in plants. The flowering time of plants is promoted by introducing a recombinant vector containing a polynucleotide encoding AGL28 protein having the amino acid sequence of SEQ ID NO:2 into Arabidopsis thaliana so as to overexpress the AGL28 gene, wherein the polynucleotide encoding AGL28 protein has the nucleotide sequence of SEQ ID NO:1; the AGL28 is isolated from Arabidopsis thaliana; and the recombinant vector is introduced into Arabidopsis thaliana by Agrobacterium sp.-mediated method, particle gun bombardment, silicon carbide whiskers, sonication, electroporation or polyethylene glycol(PEG) precipitation.
Abstract translation: 提供了通过使用AGL28基因来促进植物开花时间的方法，以通过在植物中过表达AGL28基因来增加花和种子的产量。 通过向拟南芥中导入含有编码具有SEQ ID NO：2的氨基酸序列的AGL28蛋白的多核苷酸的重组载体来促进植物的开花时间，从而过表达AGL28基因，其中编码AGL28蛋白的多核苷酸具有核苷酸序列 SEQ ID NO：1; AGL28从拟南芥中分离得到; 并通过农杆菌介导的方法，基因枪轰击，碳化硅晶须，超声处理，电穿孔或聚乙二醇（PEG）沉淀将重组载体导入拟南芥中。
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公开(公告)号：KR100637341B1
公开(公告)日：2006-10-23
申请号：KR1020030010772
申请日：2003-02-20
Applicant: 재단법인서울대학교산학협력재단
IPC: C07K14/415
CPC classification number: C12N15/827 , C07K14/415
Abstract: 본 발명은 식물의 개화시기 조절 유전자 및 이를 이용한 식물의 개화시기 조절방법에 관한 것이다. 보다 구체적으로, 본 발명은 애기장대(
Arabidopsis thaliana )로부터 분리된 식물의 개화시기 조절 유전자
LOV1 및 상기 유전자를 식물체에서 과다 발현시켜 식물체의 개화시기를 지연시키거나,
LOV1 유전자의 발현을 억제하여 식물체의 조기 개화를 유도하는 방법에 관한 것이다. 본 발명에서 분리된
LOV1 유전자 및 상기 유전자로부터 발현되는 LOV1 단백질은 식물의 개화 관련 형질의 개선 및 타 식물체에서 개화 시기 조절 유전자의 탐색 등에 유용하게 이용 될 수 있다. 또한, 본 발명에 따라 식물체의 개화시기를 촉진함으로써 꽃과 종자를 단시간 내에 생산하거나 또는 식물체의 개화시기를 지연시켜 영양생장을 지속적으로 유도함으로써 식물체로부터 잎이나 줄기와 같은 유용한 부분의 생산량을 증대시킬 수 있는 효과가 있다.
개화시기 조절, 유전자
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