公开(公告)号：KR101104817B1

公开(公告)日：2012-01-16

申请号：KR1020080091880

申请日：2008-09-19

Applicant: 전남대학교산학협력단

Inventor： 김근중 ,  정대은 ,  김일철

IPC: C12N15/52 ,  C12N15/09

Abstract: 본 발명은 서열번호 2에 기재된 아미노산 서열을 코딩하는 에스테라아제(ESTL120P) 유전자 내에 다중 클로닝 위치(multiple cloning site, MCS)가 도입되어 있는 재조합 폴리뉴클레오티드에 관한 것으로, 상기 재조합 폴리뉴클레오티드를 인디케이터(indicator)로서 사용하여 외래 유전자를 포함하는 클론을 선별하는 단계를 포함하는 재조합 유전자 클로닝 벡터의 제조방법은 기존의 인디케이터(indicator)들 보다 저가의 기질을 이용하며 짧은 배양시간을 통해 빠르고 정확하게 재조합 균체의 선별이 가능하며, PCR 증폭 산물을 직접 클로닝할 수 있는 TA 클로닝 벡터와의 접목을 통해 상업적으로 판매되는 기존의 클로닝 벡터내의 인디케이터(indicator)들을 대체 할 수 있을 것으로 기대된다.
인디케이터, 에스테라아제, 다중 클로닝, 폴리뉴클레오티드

公开(公告)号：KR1020110026965A

公开(公告)日：2011-03-16

申请号：KR1020090084841

申请日：2009-09-09

Applicant: 전남대학교산학협력단

Inventor： 김근중 ,  정대은

IPC: C12N15/52 ,  C12N15/09 ,  C12N15/63 ,  C07K19/00

Abstract: PURPOSE: A recombinant polynucleotide in which multiple cloning site is introduced into a beta glucosidase(cpGluT) gene is provided to accurately select recombinant strain. CONSTITUTION: A beta glucosidase(cpGluT) gene encodes an amino acid sequence of sequence number 2. The beta glucosidase gene comprises a base sequence of sequence number 1. A recombinant polynucleotide has multiple cloning site(MCS) in the beta glucosidase gene. A transgenic microorganism containing the recombinant polynucleotide is prepared by transforming a microorganism with a recombinant vector having the recombinant polynucleotide. The transgenic microorganism is deposited by deposit number KCTC 11500BP. A fusion protein contains the recombinant polynucleotide as a reporter.

Abstract translation: 目的：提供多克隆位点引入β-葡糖苷酶（cpGluT）基因的重组多核苷酸，以准确选择重组菌株。 构型：β葡糖苷酶（cpGluT）基因编码序列号2的氨基酸序列.β葡糖苷酶基因包含序列号1的碱基序列。重组多核苷酸在β葡糖苷酶基因中具有多个克隆位点（MCS）。 通过用具有重组多核苷酸的重组载体转化微生物来制备含有重组多核苷酸的转基因微生物。 转基因微生物通过保藏号KCTC 11500BP沉积。 融合蛋白含有作为报告物的重组多核苷酸。

公开(公告)号：KR1020100032956A

公开(公告)日：2010-03-29

申请号：KR1020080091880

申请日：2008-09-19

Applicant: 전남대학교산학협력단

Inventor： 김근중 ,  정대은 ,  김일철

IPC: C12N15/52 ,  C12N15/09

Abstract: PURPOSE: An esterase gene and recombinant polynucleotide are provided to replace an indicator in a conventional cloning vector through combining with TA cloning vector. CONSTITUTION: An esterase gene encodes an amino acid sequence of sequence number 2. The esterase gene comprises a sequence of sequence number 1. A recombinant polynucleotide has multiple cloning site in the esterase gene. The multiple cloning site is introduced one of sited from between 216th and 217th, 281th and 282th, and 306th and 307th in sequence number 2. The multi cloning site comprises a sequence of sequence number 12. The recombinant vector is TA cloning vector.

Abstract translation: 目的：提供酯酶基因和重组多核苷酸以通过与TA克隆载体结合来代替常规克隆载体中的指示剂。 构成：酯酶基因编码序列号2的氨基酸序列。酯酶基因包含序列号1的序列。重组多核苷酸在酯酶基因中具有多个克隆位点。 引入多克隆位点是从序列号2的第216位至第217位，第281位，第282位，第306位，第307位的引物。多克隆位点包含序列号12的序列。重组载体为TA克隆载体。

公开(公告)号：KR1020090108494A

公开(公告)日：2009-10-15

申请号：KR1020080033941

申请日：2008-04-11

Applicant: 전남대학교산학협력단

Inventor： 김근중 ,  이기석 ,  정대은

IPC: C12N15/52 ,  C12N9/00

Abstract: PURPOSE: A gene encoding an artificial restriction enzyme for constructing complete ORF library is provided to enhance the efficiency and screen the gene with low price in a short time. CONSTITUTION: A gene encoding an artificial restriction enzyme for constructing complete ORF library comprises: a cutting site of DNA or binding site of transcription factor; a protein binding site which recognizes transcription regulatory sequence or binds; and a protein which recognizes one selected among a promoter (-10, -35 site) of prokaryote and BRE, TATA, Inr or DPE of eukaryote.

Abstract translation: 目的：提供构建完整ORF文库的编码人工限制酶的基因，以在短时间内以低价格提高效率和筛选基因。 构成：编码构建完整ORF文库的人工限制酶的基因包括：切割位点或转录因子结合位点; 识别转录调节序列或结合的蛋白质结合位点; 和识别真核生物的原核生物和BRE，TATA，Inr或DPE的启动子（-10，-35位点）之一的蛋白质。