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公开(公告)号：KR101080288B1
公开(公告)日：2011-11-08
申请号：KR1020080108453
申请日：2008-11-03
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은새로운식물전신-발현프로모터 (constitutive promoter)에관한것으로서, 보다상세하게는애기장대 ()로부터유래한프로모터 AtUK1, 그의제조방법, 이를포함하는재조합발현벡터및 이를식용작물의형질개량에사용하는용도에관한것이다. 본발명의전신-발현프로모터는외래유전자를발현장소에상관없이발현할수 있어각종제초제저항성유전자및 리포터유전자등의전신-발현을유도하거나식량작물에서유용한형질을개량시킨형질전환식물을개발하는데 널리사용될수 있다.
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公开(公告)号：KR1020100113278A
公开(公告)日：2010-10-21
申请号：KR1020090031765
申请日：2009-04-13
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A beta-carotene hydroxylase 1 gene promoter and expression vector having the same are provided to induce expression of target protein through whole plant body. CONSTITUTION: An AtBch1 promoter for inducing constitutive expression comprises a nucleotide sequence of sequence number 1. A recombinant vector for inducing constitutive expression contains the promoter. The vector is pBGWFS7-PAtBch1 vector(KACC 95096P). A transgenic plant is prepared using transformed cells and the vector.
Abstract translation: 目的：提供β-胡萝卜素羟化酶1基因启动子及其表达载体，以通过整个植物体诱导靶蛋白的表达。 构成：用于诱导组成型表达的AtBch1启动子包含序列号1的核苷酸序列。用于诱导组成型表达的重组载体含有启动子。 载体是pBGWFS7-PAtBch1载体（KACC 95096P）。 使用转化细胞和载体制备转基因植物。
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公开(公告)号：KR100987009B1
公开(公告)日：2010-10-12
申请号：KR1020080048819
申请日：2008-05-26
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 식물체 내에 외래 유용유전자의 발현 장소를 뿌리 부분으로 제한하여 발현시키는 뿌리 특이 발현 프로모터에 관한 것으로서, 더욱 상세하게는 서열번호:1로 구성되는 뿌리 특이 발현 프로모터, 서열번호:2로 구성되는 올리고 누클레오티드 및 서열번호:3으로 구성되는 올리고누클레오티드로 이루어지는 상기 뿌리 특이 프로모터를 증폭하기 위한 프라이머, 상기 서열번호:1의 프로모터 서열을 포함하는 것을 특징으로 하는 발현벡터 pBGWFS7-PAtRA166 및 상기 발현벡터 pBGWFS7-PAtRA166 으로 형질전환된 식물세포에 관한 것이다.
뿌리특이, 프로모터, 애기장대, 조직특이, 형질전환체-
公开(公告)号：KR1020170046925A
公开(公告)日：2017-05-04
申请号：KR1020150147129
申请日：2015-10-22
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: 본발명은후자리움속검출용프라이머및 이를이용하여후자리움속을검출하는방법에관한것이다. 본발명에따른프라이머쌍을이용하면, 후자리움()속에속하는종(species) 중후자리움아르메니아쿰()만을특이적으로우수한효율로단시간내에검출할수 있다.
Abstract translation: 本发明涉及用于检测镰刀菌属的引物，以及使用该引物检测镰刀菌属的方法。 通过使用根据本发明的引物对，可以在短时间内以特别优异的效率仅检测到属于Fructus属的物种。
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15.发明公开붉은 곰팡이 병균의 리니지 구분용 미세표식자 마커 및 이러한 마커를 이용하여 붉은 곰팡이 병균의 리니지를 구분하는 방법 有权用于识别红霉病病原体的微生物标记物和使用标记鉴定红霉病病原体的方法
公开(公告)号：KR1020130058269A
公开(公告)日：2013-06-04
申请号：KR1020110124187
申请日：2011-11-25
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6895 , C12R1/77
Abstract: PURPOSE: A microsatellite marker for distinguishing lineage of red mold pathogen and a method for distinguishing lineage of red mold pathogen using the same are provided to quickly and accurately distinguish lineage of a main contamination source of complex red mold pathogen from a grain sample by analyzing PCR using FgmsAAG93. CONSTITUTION: A primer, FgmsAAG93, of sequence numbers 1 and 2 is used for specifically amplifying a microsatellite of red mold to distinguish lineage of a main contamination source among red mold pathogens. A method for distinguishing lineage of F. graminearum comprises: a step of amplifying a gene using FgmsAAG93; and a step of identifying a microsatellite site of the amplified gene. A kit for distinguishing and diagnosing lineage of the red mold pathogens contains the primer and a reagent for amplification.
Abstract translation: 目的：提供用于区分红霉病病原谱系的微卫星标记和用于区分红霉病病原体谱系的方法，通过分析PCR快速准确地区分复杂红霉病病原体的主要污染源与谷物样品的谱系 使用FgmsAAG93。 构成：序列号1和2的引物FgmsAAG93用于特异性扩增红霉菌的微卫星，以区分红霉病原体中主要污染源的谱系。 用于区分禾谷镰刀菌谱系的方法包括：使用FgmsAAG93扩增基因的步骤; 以及鉴定扩增基因的微卫星位点的步骤。 用于区分和诊断红霉病菌的谱系的试剂盒含有引物和扩增试剂。
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Patent Agency Ranking
公开(公告)号：KR1020120070931A
公开(公告)日：2012-07-02
申请号：KR1020100132464
申请日：2010-12-22
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6895 , C12R1/77
Abstract: PURPOSE: A primer pair for detecting deoxynivalenol(DON)-producing Gibberella zeae and a kit using the same are provided to quickly and accurately diagnose DON. CONSTITUTION: A primer pair for detecting DON-producing Gibberella zeae is denoted by sequence numbers 1 and 2. The Gibberella zeae is Fusarium graminearum. A kit for detecting the Gibberella zeae contains the primer pair. A method for detecting the Gibberella zeae using the primer pair comprises: a step of isolating RNA from a sample of Oryza sativa, barley, and wheat; a step of amplifying a target sequence by RT-PCR; and a step of detecting a PCR product.
Abstract translation: 目的：提供用于检测产生赤霉菌的脱氧雪腐镰刀菌烯醇（DON）和使用其的试剂盒的引物对，以快速准确地诊断DON。 构成：用于检测产生DON的赤霉菌的引物对由序列号1和2表示。玉米赤霉属是禾谷镰刀菌（Fusarium graminearum）。 用于检测玉米赤霉菌的试剂盒含有引物对。 使用引物对玉米赤霉菌进行检测的方法包括：从水稻，大麦和小麦的样品中分离RNA的步骤; 通过RT-PCR扩增靶序列的步骤; 以及检测PCR产物的步骤。
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公开(公告)号：KR1020100049335A
公开(公告)日：2010-05-12
申请号：KR1020080108451
申请日：2008-11-03
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/8216 , C12N9/20
Abstract: PURPOSE: A novel plant constitutive promoter is provided to express a foreign gene regardless of expression place and to develop transgenic plant. CONSTITUTION: A plant constitutive promoter contains partial or whole base of sequence number 1. The constitutive promoter is derived from Arabidopsis thaliana. A plant constitutive promoter, Lip2 is obtained by performing PCR of oligonucleotide of sequence numbers 6 and 7. A recombinant expression vector pBGWFS7-PAtLip3(deposit number: KACC 95086P) contains the plant constitutive promoter. The plant expression vector which is able to express a target gene is prepared using the constitutive promoter.
Abstract translation: 目的：提供一种新颖的植物组成型启动子来表达外源基因，不论其表达位置如何，并开发转基因植物。 构成：植物组成型启动子含有序列号1的部分或全部碱基。组成型启动子来源于拟南芥。 通过进行序列号6和7的寡核苷酸的PCR获得植物组成型启动子Lip2。重组表达载体pBGWFS7-PAtLip3（保藏号：KACC 95086P）含有植物组成型启动子。 使用组成型启动子制备能够表达靶基因的植物表达载体。
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公开(公告)号：KR100953763B1
公开(公告)日：2010-04-21
申请号：KR1020080023031
申请日：2008-03-12
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 식물체 내에 외래 유용유전자의 발현 장소를 뿌리 부분으로 제한하여 발현시키는 뿌리 특이 발현 프로모터에 관한 것으로서, 더욱 상세하게는 서열번호:1로 구성되는 뿌리 특이 발현 프로모터, 서열번호:2로 구성되는 올리고 누클레오티드 및 서열번호:3으로 구성되는 올리고누클레오티드로 이루어지는 상기 뿌리 특이 프로모터를 증폭하기 위한 프라이머, 상기 서열번호:1의 프로모터 서열을 포함하는 것을 특징으로 하는 발현벡터 pBGWFS7-PAtM19PK 및 상기 발현벡터 pBGWFS7-PAtM19PK으로 형질전환된 식물세포에 관한 것이다.
뿌리특이, 프로모터, 애기장대, 조직특이, 형질전환체-
19.发明授权ＯｓＷＲＫＹ６유전자를 과량 발현시켜 식물 병 저항성을증진시키는 방법, 이러한 활성을 가진 ＯｓＷＲＫＹ６유전자, 이 유전자를 포함하는 발현벡터 및 형질전환체 有权通过OSWRKY6基因的超表达，具有该活性的OSWRKY6基因，含有相同基因的表达载体和转基因植物细胞提高对植物病害的抗性的方法
公开(公告)号：KR100809671B1
公开(公告)日：2008-03-05
申请号：KR1020060091525
申请日：2006-09-21
Applicant: 대한민국(농촌진흥청장)
Abstract: A method for enhancing the resistance against plant diseases and a transformant over-expressing an OsWRKY6 gene are provided to be utilized as a molecular breeding material for developing a disease-resistant crop by having excellent bacterial blight resistance in rice as well as resistance against wide range of pathogenic bacteria range resistance, be used for various crops because other crops preserve well disease defense mechanism relating to the OsWRKY6 gene and decrease the use of pesticide due to the disease resistant species breeding, thereby improving the productivity and supplying safe agricultural products. A method for enhancing the resistance against plant diseases comprises the steps of: (a) preparing an expression vector pB2GW7-OsWRKY6 having a cleavage map depicted in Fig. 4b including an OsWRKY6 gene consisting of SEQ ID : NO. 1; and (b) introducing the expression vector into a plant cell to prepare a transformant over-expressing the OsWRKY6 gene. A transformant is transformed by the expression vector pB2GW7-OsWRKY6, wherein the transformant is selected from the group consisting of a rice cell, a cabbage cell, a hot pepper cell, a potato cell and a rape cell. Further, the plant diseases are selected from a group consisting of Bacterial blight, rice blast disease, Rice stripe tenuivirus, Rhizoctonia solani, Rice Black-Streaked Dwarf Virus and Mycosphaerella leaf spot.
Abstract translation: 提供了增强抗病性的方法和过表达OsWRKY6基因的转化体，用作通过在水稻中具有优异的抗病性以及耐宽泛的种类来开发抗病作物的分子育种材料 的病原菌种类抗性，用于各种作物，因为其他作物保存了与OsWRKY6基因相关的良好的疾病防治机制，并且由于抗病种的繁殖而减少了农药的使用，从而提高了生产力并提供了安全的农产品。 一种提高对植物病害抗性的方法包括以下步骤：（a）制备具有图1所示切割图的表达载体pB2GW7-OsWRKY6。 4b包括由SEQ ID NO：1组成的OsWRKY6基因。 1; 和（b）将表达载体导入植物细胞以制备过表达OsWRKY6基因的转化体。 通过表达载体pB2GW7-OsWRKY6转化转化体，其中转化体选自水稻细胞，卷心菜细胞，辣椒细胞，马铃薯细胞和强奸细胞。 此外，植物疾病选自细菌性枯萎病，稻瘟病，水稻条纹病毒，立枯丝核菌，水稻黑条纹矮病毒和丝状霉病叶斑病。
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