公开(公告)号：KR100450145B1

公开(公告)日：2004-09-30

申请号：KR1020010055931

申请日：2001-09-11

Applicant: 정필훈

Inventor： 정필훈 ,  정한울 ,  정한솔 ,  정종훈 ,  정연훈 ,  김은석 ,  홍동련

IPC: A61L27/12

Abstract: PURPOSE: Provided is a bioabsorptive ceramic-polymer complex material for tissue engineering with teeth apatite and polymer used which mixes teeth apatite, polymer for tissue engineering and solvent to keep porosity on the surface and section so that it improves humidity absorption rate. CONSTITUTION: The production process of the bioabsorptive ceramic-polymer complex material for tissue engineering with teeth apatite and polymer used comprises the steps of: (i) extracting an inorganic material from teeth of human or animals and crushing them to prepare teeth apatite; (ii) selecting one material from PGA, PLA, PLLA, PLGA, chitosan and PHBV as bioabsorptive polymer; (iii) selecting one solvent from methylene chloride, chloroform, DMSO and week acid; and (iv) mixing 60-90wt% of bioabsorptive polymer and 10-40wt% of teeth apatite under the selected solvent to produce a ceramic-polymer complex material.

Abstract translation: 用途：提供一种用于组织工程的生物吸收性陶瓷 - 聚合物复合材料，其具有牙齿磷灰石和使用的聚合物，其混合牙齿磷灰石，用于组织工程的聚合物和溶剂，以保持表面和截面上的孔隙率，从而提高吸湿率。 本发明提供了一种生物吸附性陶瓷 - 聚合物复合材料的制备方法，所述生物吸附性陶瓷 - 聚合物复合材料具有用于牙齿磷灰石和聚合物的组织工程，包括以下步骤：（i）从人或动物的牙齿中提取无机材料并粉碎以制备牙齿磷灰石; （ii）从PGA，PLA，PLLA，PLGA，壳聚糖和PHBV中选择一种作为生物吸收聚合物的材料; （iii）从二氯甲烷，氯仿，DMSO中选择一种溶剂并且一周酸; 和（iv）在所选溶剂下混合60-90重量％的生物吸收性聚合物和10-40重量％的牙齿磷灰石以制备陶瓷 - 聚合物复合材料。

公开(公告)号：KR1020030071925A

公开(公告)日：2003-09-13

申请号：KR1020020011191

申请日：2002-03-02

Applicant: 정필훈

Inventor： 정필훈 ,  박근홍 ,  정종훈 ,  정연훈

IPC: C12M3/00

CPC classification number: C12M35/02

Abstract: PURPOSE: A method and an apparatus for culturing a nerve cell by electronic stimulus are provided, thereby forming synapsis and nerve circuit between axons. CONSTITUTION: A method for culturing a nerve cell by electronic stimulus comprises adhering a nerve cell on the conductive polymer-coated silicone board which is coated with gold, and culturing the nerve cell, wherein the nerve cell adhered to the silicone board is subjected to voltage in the presence of a culturing medium during the cultivation to proliferate the nerve cell, and to induce differentiation and axons; the conductive polymer is polypyrrol and coated on the gold-coated silicone board using electric polymerization; and the nerve cell is cultured under voltage of 0.01 to 1.0 V for 1 to 4 minutes. An apparatus for culturing a nerve cell by electronic stimulus comprises a culturing vessel; a board for adhering the nerve cell; electrodes; and a pseudo electrode for maintaining the voltage of the positive(+) electrode to be constant, wherein the board is prepared by coating a gold-coated silicone board with the conductive polymer.

Abstract translation: 目的：提供通过电子刺激培养神经细胞的方法和装置，从而在轴突之间形成突触和神经电路。 构成：通过电子刺激培养神经细胞的方法包括将神经细胞粘附在涂有金的导电聚合物涂覆的硅树脂板上，并培养神经细胞，其中附着在硅树脂基板上的神经细胞经受电压 在培养期间培养培养基的存在下使神经细胞增殖，并诱导分化和轴突; 导电聚合物是聚吡咯，并使用电聚合涂覆在镀金硅胶板上; 将神经细胞在0.01〜1.0V的电压下培养1〜4分钟。 用于通过电子刺激培养神经细胞的装置包括培养容器; 用于粘附神经细胞的板; 电极; 以及用于将正（+）电极的电压维持为恒定的伪电极，其中通过用导电聚合物涂覆镀金的硅树脂板来制备板。

公开(公告)号：KR100488405B1

公开(公告)日：2005-05-11

申请号：KR1020020025143

申请日：2002-05-07

Applicant: 정필훈 ,  정종훈

Inventor： 정필훈 ,  정종훈 ,  정연훈 ,  정한울

IPC: A61L27/24

Abstract: 본 발명은 생체흡수성 신경 도관 및 이의 제조 방법에 관한 것으로서, 보다 상세하게는 생체 흡수성 폴리머, 콜라겐, 알지네이트 및 생체 흡수성세라믹으로 구성된 군으로부터 선택된 도관형성물을 그 재질로 가지는 도관으로서, 터널형태, 구공탄 형태, 멍석 말은 형태를 가지며 그 형태의 내부에 형성된 키토산 코팅층을 포함하거나 키토산을 함유하는 생체흡수성 신경 도관 및 이의 제조 방법에 관한 것이다. 본 발명의 인조신경 도관은 내경과 외경을 자유롭게 조절하여 제작될 수 있으며 결손된 신경조직을 연결하고 신경섬유를 재생하는 통로 역할을 하며, 임상적으로도 안면신경 분지나 하치조 신경 및 설신경 등의 기타 신경결손 부위 회복에 유용하게 사용될 수 있다.

公开(公告)号：KR1020030087196A

公开(公告)日：2003-11-14

申请号：KR1020020025143

申请日：2002-05-07

Applicant: 정필훈 ,  정종훈

Inventor： 정필훈 ,  정종훈 ,  정연훈 ,  정한울

IPC: A61L27/24

Abstract: PURPOSE: Provided are a bioabsorbing nerve conduit comprising, as an active material, toothapatite, polymer material or chitosan, which is a bioabsorbing ceramic, and a method for manufacturing the same. CONSTITUTION: The bioabsorbing nerve conduit consists of tube components or bioabsorbing polymer materials selected from the group consisting of collagen, alginate and ceramic, wherein coating layer is positioned inside the tube. The tube components further comprise polymer material. The ceramic is selected from the group consisting of toothapatite and bio-apatite. The polymer material is at least one selected from the group consisting of PHBV(poly-hydroxybutyrate-co-hydroxyvalerate), polyglycolic acid(PGA), polylactic acid(PLA), PLLA(poly(L-lactic acid)), polylactic-glycolic acid(PLGA), and chitosan.

Abstract translation: 目的：提供一种生物吸收神经导管，其包含作为活性物质的作为生物吸收性陶瓷的作为活性物质的山茶钙，聚合物材料或壳聚糖及其制造方法。 构成：生物吸收神经导管由选自由胶原，藻酸盐和陶瓷组成的组的管组分或生物吸收性聚合物材料组成，其中涂层位于管内。 管组件还包括聚合物材料。 陶瓷选自由硅藻土和生物磷灰石组成的组。 聚合物材料为选自PHBV（聚羟基丁酸酯 - 共 - 羟基戊酸酯），聚乙醇酸（PGA），聚乳酸（PLA），PLLA（聚（L-乳酸）），聚乳酸 - 乙醇酸 酸（PLGA）和壳聚糖。

公开(公告)号：KR1020030022594A

公开(公告)日：2003-03-17

申请号：KR1020010055931

申请日：2001-09-11

Applicant: 정필훈

Inventor： 정필훈 ,  정한울 ,  정한솔 ,  정종훈 ,  정연훈 ,  김은석 ,  홍동련

IPC: A61L27/12

Abstract: PURPOSE: Provided is a bioabsorptive ceramic-polymer complex material for tissue engineering with teeth apatite and polymer used which mixes teeth apatite, polymer for tissue engineering and solvent to keep porosity on the surface and section so that it improves humidity absorption rate. CONSTITUTION: The production process of the bioabsorptive ceramic-polymer complex material for tissue engineering with teeth apatite and polymer used comprises the steps of: (i) extracting an inorganic material from teeth of human or animals and crushing them to prepare teeth apatite; (ii) selecting one material from PGA, PLA, PLLA, PLGA, chitosan and PHBV as bioabsorptive polymer; (iii) selecting one solvent from methylene chloride, chloroform, DMSO and week acid; and (iv) mixing 60-90wt% of bioabsorptive polymer and 10-40wt% of teeth apatite under the selected solvent to produce a ceramic-polymer complex material.

Abstract translation: 目的：提供一种用于组织工程的生物吸收性陶瓷 - 聚合物复合材料，其具有使用的磷灰石和聚合物，其将牙齿磷灰石，用于组织工程和溶剂的聚合物混合以在表面和部分上保持孔隙率，从而改善吸湿率。 构成：用于牙齿磷灰石和聚合物的组织工程生物吸收性陶瓷 - 聚合物复合材料的生产过程包括以下步骤：（i）从人或动物的牙齿中提取无机材料并粉碎以制备牙齿磷灰石; （ii）从PGA，PLA，PLLA，PLGA，壳聚糖和PHBV中选择一种生物吸收性聚合物; （iii）从二氯甲烷，氯仿，DMSO和一周酸中选择一种溶剂; 和（iv）在选择的溶剂下混合60-90重量％的生物吸收聚合物和10-40重量％的磷灰石，以制备陶瓷 - 聚合物复合材料。