公开(公告)号：KR100450633B1

公开(公告)日：2004-09-30

申请号：KR1020020011191

申请日：2002-03-02

Applicant: 정필훈

Inventor： 정필훈 ,  박근홍 ,  정종훈 ,  정연훈

IPC: C12M3/00

CPC classification number: C12M35/02

Abstract: PURPOSE: A method and an apparatus for culturing a nerve cell by electronic stimulus are provided, thereby forming synapsis and nerve circuit between axons. CONSTITUTION: A method for culturing a nerve cell by electronic stimulus comprises adhering a nerve cell on the conductive polymer-coated silicone board which is coated with gold, and culturing the nerve cell, wherein the nerve cell adhered to the silicone board is subjected to voltage in the presence of a culturing medium during the cultivation to proliferate the nerve cell, and to induce differentiation and axons; the conductive polymer is polypyrrol and coated on the gold-coated silicone board using electric polymerization; and the nerve cell is cultured under voltage of 0.01 to 1.0 V for 1 to 4 minutes. An apparatus for culturing a nerve cell by electronic stimulus comprises a culturing vessel; a board for adhering the nerve cell; electrodes; and a pseudo electrode for maintaining the voltage of the positive(+) electrode to be constant, wherein the board is prepared by coating a gold-coated silicone board with the conductive polymer.

Abstract translation: 目的：提供一种通过电子刺激来培养神经细胞的方法和装置，从而形成轴突之间的联会和神经回路。 组成：通过电子刺激培养神经细胞的方法包括将神经细胞粘附在涂有金的导电聚合物涂覆的硅酮板上，并培养神经细胞，其中粘附在硅酮板上的神经细胞受到电压 在培养期间存在培养基以增殖神经细胞并诱导分化和轴突; 导电聚合物是聚吡咯并使用电聚合涂覆在镀金硅树脂板上; 神经细胞在0.01〜1.0V的电压下培养1〜4分钟。 通过电子刺激培养神经细胞的装置包括培养容器; 用于粘附神经细胞的板; 电极; 以及用于保持正（+）电极的电压恒定的伪电极，其中该基板通过用导电聚合物涂布镀金硅酮板来制备。

公开(公告)号：KR1020030071925A

公开(公告)日：2003-09-13

申请号：KR1020020011191

申请日：2002-03-02

Applicant: 정필훈

Inventor： 정필훈 ,  박근홍 ,  정종훈 ,  정연훈

IPC: C12M3/00

CPC classification number: C12M35/02

Abstract: PURPOSE: A method and an apparatus for culturing a nerve cell by electronic stimulus are provided, thereby forming synapsis and nerve circuit between axons. CONSTITUTION: A method for culturing a nerve cell by electronic stimulus comprises adhering a nerve cell on the conductive polymer-coated silicone board which is coated with gold, and culturing the nerve cell, wherein the nerve cell adhered to the silicone board is subjected to voltage in the presence of a culturing medium during the cultivation to proliferate the nerve cell, and to induce differentiation and axons; the conductive polymer is polypyrrol and coated on the gold-coated silicone board using electric polymerization; and the nerve cell is cultured under voltage of 0.01 to 1.0 V for 1 to 4 minutes. An apparatus for culturing a nerve cell by electronic stimulus comprises a culturing vessel; a board for adhering the nerve cell; electrodes; and a pseudo electrode for maintaining the voltage of the positive(+) electrode to be constant, wherein the board is prepared by coating a gold-coated silicone board with the conductive polymer.

Abstract translation: 目的：提供通过电子刺激培养神经细胞的方法和装置，从而在轴突之间形成突触和神经电路。 构成：通过电子刺激培养神经细胞的方法包括将神经细胞粘附在涂有金的导电聚合物涂覆的硅树脂板上，并培养神经细胞，其中附着在硅树脂基板上的神经细胞经受电压 在培养期间培养培养基的存在下使神经细胞增殖，并诱导分化和轴突; 导电聚合物是聚吡咯，并使用电聚合涂覆在镀金硅胶板上; 将神经细胞在0.01〜1.0V的电压下培养1〜4分钟。 用于通过电子刺激培养神经细胞的装置包括培养容器; 用于粘附神经细胞的板; 电极; 以及用于将正（+）电极的电压维持为恒定的伪电极，其中通过用导电聚合物涂覆镀金的硅树脂板来制备板。