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公开(公告)号：KR101518200B1
公开(公告)日：2015-06-05
申请号：KR1020130088031
申请日：2013-07-25
Applicant: 조선대학교산학협력단
Abstract: 본발명은스트렙토마이세스종 균주유래의신규자일란분해효소및 농업부산물분해에서의응용에관한것이다. 본발명에따른 EX624 자일란분해효소는쉽게구할수 있고값싼농산부산물인밀기울로보충한배양배지중에서스트렙토마이세스종(sp.) CS624를배양함으로써경제적으로생산되었다. 생화학적특성분석결과 EX624 자일란분해효소가셀룰라아제와무관하고, 산성및 알칼리성 pH의넓은범위에서안정되고, 적절하게열안정성인것으로나타났다. 또한, 정제 EX624 자일란분해효소를사용한너도밤나무자일란의효소적가수분해는이것이자일로올리고사카라이드생산에잠재적으로사용될수 있는것으로밝혀졌다.
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12.发明公开
公开(公告)号：KR1020130073230A
公开(公告)日：2013-07-03
申请号：KR1020110140964
申请日：2011-12-23
Applicant: 조선대학교산학협력단
Abstract: PURPOSE: A cold-adapted lipase derived from mesophilic Streptomyces sp. which catalyzes biodiesel production is provided to selectively hydrolyze p-nitrophenyl dodecanoate (C_10), to non-specifically hydrolyze triolein, to release 1,2- and 1,3-diolein, and to efficiently produce biofuel. CONSTITUTION: Cold-adapted lipase LP28 which catalyzes biodiesel production is isolated from Streptomyces sp. The molecular weight of lipase LP28 measured by SDS-PAGE is 32,400 Da. The optimal temperature and pH are 30 deg. C and pH 8.0. Lipase LP28 selectively hydrolyzes p-nitrophenyl dodecanoate (C_10), non-specifically hydrolyzes triolein, releases 1,2- and 1,3-diolein, and quickly produce biodiesel using palm oil and methanol.
Abstract translation: 目的：从嗜温链霉菌属（Streptomyces sp。）衍生的冷适应脂肪酶。 提供催化生物柴油生产以选择性水解对硝基苯基十二酸酯（C_10），以非特异性水解三油酸酯以释放1,2-和1,3-二油烯，并有效生产生物燃料。 构成：催化生物柴油生产的冷适应脂肪酶LP28从链霉菌属（Streptomyces sp。 通过SDS-PAGE测定的脂肪酶LP28的分子量为32,400Da。 最佳温度和pH值为30度。 C和pH 8.0。 脂肪酶LP28选择性水解对硝基苯基十二酸酯（C_10），非特异性水解三油酸酯，释放1,2-和1,3-二油烯，并使用棕榈油和甲醇快速生产生物柴油。
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公开(公告)号：KR1020120111091A
公开(公告)日：2012-10-10
申请号：KR1020110029387
申请日：2011-03-31
Applicant: 조선대학교산학협력단
CPC classification number: Y02A50/473 , Y02A50/481 , A61K35/74 , A61K9/0019 , A61K9/0053
Abstract: PURPOSE: A novel Bacillus amyloliquefaciens CS61 culture is provided to ensure excellent antibacterial function, DPPH radical removal ability, and high reducing ability. CONSTITUTION: An antioxidant contains Bacillus amyloliquefaciens CS61(KCTC 11901BP) culture as an active ingredient. An anti-inflammatory agent contains the cutlure as an active ingredient. A pharmaceutical composition for suppressing the growth of antibiotic resistant pathogenic microorganism or enteric harmful microorganism contains the culture as an active ingredient. The antibiotic resistant pathogenic microorganism is Methicillin Resistant Staphylococcus aureus(MRSA) or vancomycin resistant enterococcus(VRE). The enteric harmful microorganism is E.coli, Staphylococcus aurues, Enterococcus, or Salmonella.
Abstract translation: 目的：提供一种新型解淀粉芽孢杆菌CS61培养物，以确保优异的抗菌功能，DPPH自由基去除能力和高还原能力。 构成：抗氧化剂含有解淀粉芽孢杆菌CS61（KCTC 11901BP）培养物作为活性成分。 抗炎剂含有作为活性成分的切口。 用于抑制抗生素抗性致病微生物或肠道有害微生物生长的药物组合物含有培养物作为活性成分。 耐药性致病微生物是耐甲氧西林金黄色葡萄球菌（MRSA）或万古霉素耐药性肠球菌（VRE）。 肠道有害微生物是大肠杆菌，金黄色葡萄球菌，肠球菌属或沙门氏菌。
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Patent Agency Ranking
公开(公告)号：KR101780229B1
公开(公告)日：2017-09-21
申请号：KR1020160113693
申请日：2016-09-05
Applicant: 조선대학교산학협력단
CPC classification number: C12N1/20 , C12N9/2402 , C12R1/125
Abstract: 본발명은발효음식김치로부터단리된바실러스섭틸리스아종인아쿠오소룸 CSB31 유래의극 알칼리성만난분해효소및 그의용도에관한것이다. 본발명에따른 MnB31은무 셀룰로오스성이고그의농업폐기물을부가가치생성물로의재순환시키는능력은저비용및 무공해환경을유지시키는잠재적능력의측면에서매우유망하다. 그의할로-관용성, 우레아내성및 프로테아제안정성은농업폐기물이생분해에있어서그의용도를지원한다. MnB31은만난에대해높은특이성을가지므로, 식품및 제약산업에서프리바이오틱으로서고도로유용하고, 농업폐기물의적절한이용뿐만아니라, 제지및 펄프산업에서바이오-표백, 바이오연료및 직물산업등의몇몇바이오기술및 산업적용도에아주유용하게사용될수 있다.
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公开(公告)号：KR101541787B1
公开(公告)日：2015-08-04
申请号：KR1020130088091
申请日：2013-07-25
Applicant: 조선대학교산학협력단
Abstract: 본발명은스트렙토마이세스종 균주유래의알칼리저항성신규자일란분해효소및 그의용도에관한것이다. 본발명에따른자일란분해효소, Xyn628은 pH 5 내지 13의넓은범위에서안정하였으며, 이는알칼리내성자일란분해효소임을나타낸다. Xyn628는자일란으로부터주요가수분해생성물로서자일로바이오스및 자일로트리오스를생산하였는데이는엔도타입자일란분해효소임을의미한다. Xyn628는셀룰라아제활성이결핍된것으로나타났으며, 저분자량및 다양한생화학적특성으로인하여바이오표백및 기타바이오산업에있어서이 효소가더 적합하게이용될수 있다.
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公开(公告)号：KR1020150012507A
公开(公告)日：2015-02-04
申请号：KR1020130088091
申请日：2013-07-25
Applicant: 조선대학교산학협력단
Abstract: The present invention relates to a novel alkaline resistance xylanase derived from stereptomyces sp. CS628 strain and uses thereof. According to the present invention, the xylanase, Xyn628 is stable in wide range of PH 5 to 13. It means that Xyn628 is an alkaline resistance xylanase. Xyn628 produces xylobios and xylotrios as main hydrolysis products from xylan. It means that Xyn628 is an endo-type xylanase. It is confirmed that Xyn628 lacks cellulase activity. The enzyme can be used in bio bleaching and other bio industries because of low molecular weight and various biochemical properties.
Abstract translation: 本发明涉及一种来自立体破乳菌属的新型耐碱性木聚糖酶。 CS628菌株及其用途。 根据本发明，木聚糖酶Xyn628在PH5〜13的宽范围内稳定。这意味着Xyn628是耐碱木聚糖酶。 Xyn628生产木糖和木三糖作为木聚糖的主要水解产物。 这意味着Xyn628是内切型木聚糖酶。 证实Xyn628缺乏纤维素酶活性。 由于低分子量和各种生物化学性质，该酶可用于生物漂白和其他生物工业。
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17.发明授权
公开(公告)号：KR101350302B1
公开(公告)日：2014-01-22
申请号：KR1020110140964
申请日：2011-12-23
Applicant: 조선대학교산학협력단
Abstract: 본 발명은 중온성 스트렙토마이세스속 유래의 바이오디젤 생산을 촉매하는 신규 냉온-적응성 지질분해효소 및 그의 생산 방법에 관한 것이다. 본 발명에 따른 지질분해효소는 중간 크기의 사슬 기질인
p -니트로페닐 데카노에이트 (C
10 )를 선택적으로 가수분해하고, 트리올레인을 비특이적으로 가수분해하여 1,2- 및 1,3-디올레인 모두를 방출하고, 팜유 및 메탄올을 사용하여 바이오디젤 생산을 신속하게 촉매하였으므로 바이오연료 생산에 효율적으로 이용될 수 있다.-
公开(公告)号：KR1020130072538A
公开(公告)日：2013-07-02
申请号：KR1020110140010
申请日：2011-12-22
Applicant: 조선대학교산학협력단
Abstract: PURPOSE: An antibacterial compound produced by Streptomyces sp. CS392 is provided to ensure various antibacterial activities against gram-positive bacteria and gram-negative bacteria such as Bacillus subtilis and Mycobacterium smegmatis, thereby being used in a therapeutic agent. CONSTITUTION: Streptomyces sp. CS392 (KCTC 12091BP) is isolated from soil. A method for preparing an antibacterial composition from the strain comprises the steps of: culturing a large amount of the strain; collecting the cultured broth, extracting with ethyl acetate, and drying; purifying by a silica gel column and eluting active ingredients by hexane-ethyl acetate to isolate and purify the antibacterial compounds; performing reverse phase-C18 silica gel column of the compounds; and purifying the compounds using water (0.01% formic acid)-acetonitrile (4:6).
Abstract translation: 目的：由链霉菌（Streptomyces sp。）生产的抗菌化合物 提供CS392以确保针对革兰氏阳性细菌和革兰氏阴性菌如枯草芽孢杆菌和耻垢分枝杆菌的抗菌活性，从而用于治疗剂。 构成：链霉菌 CS392（KCTC 12091BP）从土壤中分离。 从菌株制备抗菌组合物的方法包括以下步骤：培养大量菌株; 收集培养的肉汤，用乙酸乙酯萃取，干燥; 用硅胶柱纯化，用己烷 - 乙酸乙酯洗脱活性成分，分离纯化抗菌化合物; 进行化合物的反相C18硅胶柱; 并用水（0.01％甲酸） - 乙腈（4:6）纯化化合物。
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