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公开(公告)号：KR1020150012484A
公开(公告)日：2015-02-04
申请号：KR1020130088031
申请日：2013-07-25
Applicant: 조선대학교산학협력단
Abstract: The present invention relates to a novel xylanase derived from Streptomyces sp. strain, and to applications to agricultural by-product decomposition. According to the present invention, EX624 xylanase is economically produced by culturing Streptomyces sp. CS624 in a culturing medium comprising wheat bran which is easily obtained and is a low-priced agricultural by-product. As a result of biochemical property analysis, EX624 xylanase is irrelevant to cellulase, is stable in a wide range of acidic and alkaline PH, and is thermally stable properly. Also, enzymatic hydrolysis of beech xylanase using purified EX624 xylanase can be used for producing xylooligosaccharide.
Abstract translation: 本发明涉及一种衍生自链霉菌属（Streptomyces sp。）的新型木聚糖酶。 应变，以及农业副产品分解的应用。 根据本发明，EX624木聚糖酶通过培养链霉菌（Streptomyces sp。）经济地生产。 CS624在包含容易获得的麦麸的培养基中，是低价农业副产物。 作为生物化学性质分析的结果，EX624木聚糖酶与纤维素酶无关，在广泛的酸性和碱性PH范围内是稳定的，并且是热稳定的。 此外，使用纯化的EX624木聚糖酶的山毛榉木聚糖酶的酶水解可以用于制备低聚木糖。
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公开(公告)号：KR1020130072533A
公开(公告)日：2013-07-02
申请号：KR1020110140000
申请日：2011-12-22
Applicant: 조선대학교산학협력단
Abstract: PURPOSE: A lipase produced by Streptomyces sp. is provided to catalyze transesterification for producing biodiesel and to properly produce biodiesel. CONSTITUTION: A lipase for producing biodiesel is isolated from Streptomyces sp. (CS326) and is identified to have a single protein band through SDS-PAGE. The molecular weight of the lipase is 17 kDa. The optimal reaction temperature and pH of the lipase are 40 deg. C and pH 7.0. The lipase is a heat resistant enzyme. The lipid decomposition activity is increased to 100% by adding Triton X-100 and is suppressed to 5% by adding SDS. The lipid decomposition activity is completely suppressed in deoxycholic acid and is suppressed by 50% using EDTA and EGTA. The lipid decomposition activity is suppressed by Mg^2+, Fe^2+, Na^+, Mn^2+, Ca^2+, and Co^2+. The rate constant is Km=0.24mM and V_max=4.6mM/min/mg using Lineweaver-Burk plot.
Abstract translation: 目的：由链霉菌属（Streptomyces sp。）生产的脂肪酶 被提供用于催化酯交换以生产生物柴油并适当地生产生物柴油。 构成：用于生产生物柴油的脂肪酶是从链霉菌属（Streptomyces sp。 （CS326），并通过SDS-PAGE鉴定为具有单个蛋白质条带。 脂肪酶的分子量为17 kDa。 脂肪酶的最佳反应温度和pH为40度。 C和pH 7.0。 脂肪酶是耐热酶。 通过加入Triton X-100将脂质分解活性提高至100％，并通过加入SDS将其抑制至5％。 脂肪分解活性在脱氧胆酸中完全抑制，并使用EDTA和EGTA抑制50％。 脂肪分解活性受到Mg ^ 2 +，Fe ^ 2 +，Na +，Mn ^ 2 +，Ca ^ 2 +和Co ^ 2 +的抑制。 使用Lineweaver-Burk图，速率常数为Km = 0.24mM，V_max = 4.6mM / min / mg。
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公开(公告)号：KR101518200B1
公开(公告)日：2015-06-05
申请号：KR1020130088031
申请日：2013-07-25
Applicant: 조선대학교산학협력단
Abstract: 본발명은스트렙토마이세스종 균주유래의신규자일란분해효소및 농업부산물분해에서의응용에관한것이다. 본발명에따른 EX624 자일란분해효소는쉽게구할수 있고값싼농산부산물인밀기울로보충한배양배지중에서스트렙토마이세스종(sp.) CS624를배양함으로써경제적으로생산되었다. 생화학적특성분석결과 EX624 자일란분해효소가셀룰라아제와무관하고, 산성및 알칼리성 pH의넓은범위에서안정되고, 적절하게열안정성인것으로나타났다. 또한, 정제 EX624 자일란분해효소를사용한너도밤나무자일란의효소적가수분해는이것이자일로올리고사카라이드생산에잠재적으로사용될수 있는것으로밝혀졌다.
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