Abstract:
PURPOSE: A mass reinforcing method of gold nano-particles due to light irradiation, a biosensor, and biomolecule combining detection method using the same are provided to reinforce mass of the gold nano-particles by using an optical irradiation method. CONSTITUTION: A mass reinforcing method of gold nano-particles due to light irradiation comprises the following steps: irradiating a mixture including gold nano-particles(13) and reinforcing metal component(15) with light; and increasing the mass of the gold nano-particles by reducing thereof on the surface of the gold nano-particles. The light is ultraviolet ray. The metal reinforcing component is metallic ion. A bio molecular combining detection method comprises the following steps: reacting target bio molecular with bio sensors which detect physical property changes according to bondage of the target bio molecular; combining the gold nano-particle to the target bio molecular; dipping the bio sensor into a mixture which includes the metal reinforcing components and irradiating light; and detecting physical property change of the gold nano-particles.
Abstract:
A sensor and a method for detecting antigen-antibody reaction are provided to identify the binding of a fluorescence-unlabeled protein such as the binding of the antigen-antibody. A sensor for detecting antigen-antibody reaction is characterized in that a porous thin film is coated on a quartz crystal microbalance, wherein the porous thin film is a sol-gel porous thin film and includes an antigen or an antibody, or a protein non-specific binding inhibitor. To detect the protein binding, a porous thin film is coated on a microbalance, wherein the porous thin film has a specific protein binding portion. To detect the antigen-antibody reaction, weight change before and after the antigen-antibody reaction is detected by using the microbalance. Further, the antigen-antibody has non-fluorescence property.
Abstract:
본 발명은 바이오 물질 검출 방법 및 장치에 관한 것으로서, 보다 상세하게는 pH meter 혹은 pH indicator와 같은 상업용 pH 측정장치를 이용하여 바이오 물질을 신속하게 정량 검출하는 방법에 관한 것이다. 본 발명은 바이오물질-자성나노입자-아세틸콜린에스테라제 복합체를 이용하여 에스테르화합물을 포함하는 표준 시약의 pH를 변화시키는 방법을 제공한다.
Abstract:
The present invention relates to a method for detecting food borne pathogens and, more specifically, to a method for rapidly and quantitatively isolating contents of food borne pathogens contaminated in foods. In order to solve the assignment, the method according to the present invention comprises the following steps: adding magnetic nanoparticles capable of combining with bacteria to samples which measure the bacteria, and combining the magnetic nanoparticles with the bacteria; isolating the magnetic nanoparticles; separating the magnetic nanoparticles combined with the bacteria from magnetic nanoparticles not combined with the bacteria by transmitting the magnetic nanoparticles isolated using magnetism through a high viscous liquid; and quantifying the magnetic nanoparticles combined with the bacteria. As in the present invention, the method for isolating the magnetic nanoparticles using magnetism from high viscous liquid clearly isolates the magnetic nanoparticles with which bacteria are not combined from the magnetic nanoparticles with which bacteria are combined while showing band shapes in the high viscous liquid. If centrifugal separator is used, separation capability between nanoparticles is decreased. In addition, the method according to the present invention does not require a separate device such as the centrifugal separator, except magnetic nanoparticles and magnets.