Abstract:
본 발명의 일 측면에 따른 미세유체칩은 시료와 시약을 혼합하는 환원채널과 시약들을 혼합하는 시약 혼합채널, 및 일측 단부에 상기 환원채널과 상기 시약 혼합채널이 병렬로 연결되고 타측 단부에 배출구가 연결된 검출채널이 형성된 제1 기판, 및 상기 검출채널과 마주하며 빛이 투과하도록 형성된 검출부를 구비하며 상기 제1기판에 결합되는 제2기판을 포함한다.
Abstract:
PURPOSE: A capillary electrophoresis electrochemical detection apparatus and an analysis method using the same are provided to detect and analyze a small amount of material with the high sensitivity. CONSTITUTION: A capillary electrophoresis electrochemical detection apparatus comprises a division channel(112), a blank channel(114), a working electrode(116), and a reference electrode(118). A sample and a first buffer solution are delivered to the division channel, and the sample and the first buffer solution are mixed by gradually changing the voltage applied to a first buffer solution repository(108) and a sample repository(104). A reference solution and a second buffer solution are delivered to the blank channel, and the reference solution and the second buffer solution are mixed by gradually changing the voltage applied to a second buffer solution repository(110) and a blank repository(106). The working electrode detects electrochemical signals of the sample and the first buffer solution delivered from the division channel to a nano band electrode. The reference electrode detects electrochemical signals of the reference solution and the second buffer solution delivered from the blank channel to the nano band electrode.
Abstract:
PURPOSE: A micro derivation reactor for a high-sensitivity analysis and a method thereof are provided to reduce the loss of sensitivity by a dispersion during a derivatizing process and an inserting process through a phase separation method. CONSTITUTION: A micro derivation reactor comprises the following: a capillary tip(106) including a capillary(104) to inject a sample; and an elastic polymer substrate(108) including an internal channel(112) connected with the capillary for separating the sample into ionic liquid(102), fixing and derivatizing. A method of a micro derivation comprises the following steps: filling an electrolytic solution to the capillary using the internal channel; fixing the sample by injecting the sample and the ionic liquid, using an electric field; and derivatizing the sample and electrically separating.
Abstract:
A method for preparing surface-modified microchannels is provided to ensure excellent hydrophile property and fast electro osmotic flow, high organic solvent resistance and low absorption for neutral materials. A method for preparing surface-modified microchannels comprises (i) a step of manufacturing the sol gel solution by performing the sol-gel reaction about alkoxysilane-containing precursor solution; and (ii) a step of contacting the sol gel solution manufactured from the (i) step on he surface of the oxidized polydimethylsiloxane microchannel.
Abstract:
핵산 시료 중 미량만을 주입하여 분석하는 단계를 포함하여, 분석에 사용된 미량의 핵산 시료를 제외한 사용되지 않은 대부분의 핵산 시료를 형광물질에 오염되지 않은 순수한 산물로 얻을 수 있는 것을 특징으로 하는 핵산 분석 방법 및 상기와 같은 핵산 분석 방법이 가능한 핵산 분석용 마이크로칩이 제공된다. 상기 핵산 분석 방법은 서로 다른 2종 이상의 핵산 시료를 순차적으로 주입함으로써, 서로 다른 2종 이상의 핵산 시료를 연속적으로 분석 가능한 것일 수 있다..
Abstract:
A microfluidic chip having a flow cell, according to one aspect of the present invention, comprises: a first substrate having a sample injection hole, a light introduction hole communicating with the sample injection hole, a detection hole which is disposed at a distance from the light introduction hole; a second substrate which is coupled to the first substrate and has a light introduction part facing the light introduction hole such that the light from a light source transmits therethrough and a detection part facing the detection hole such that the light from the light source transmits therethrough; and a flow cell having one side end portion connected to the light introduction hole and the other side end portion connected to the detection hole, wherein the first substrate and the second substrate are made from a material which can absorb light.
Abstract:
The present invention provides: a method of nucleic acid analysis which includes a step of analyzing a small amount of a nucleic acid sample, and is capable of obtaining pure products from most of the nucleic acid sample which is not used for the analysis and is not contaminated by fluorescent materials; and a microchip for nucleic acid analysis capable of performing said method. The method of nucleic acid analysis can successively analyze more than two different kinds of nucleic acid samples by successively injecting the nucleic acid samples.
Abstract:
본 발명은 시료 분리 기술에 관한 것으로, 물에 섞이지 않는 이온성 액체를 이용하여 분절채취 된 시료를 마이크로칩에 모세관 팁을 통해 채널내로 주입하여 전기이동분석을 수행하기 위한 것으로서, 마이크로 칩과 시료 주입 모세관 팁의 내벽을 서로 다른 재료로 코팅하여 주입부분에서 분절 상태를 계속 유지할 수 있도록 하고, 분리채널이 시작되는 기점에서 시료가 이온성 액체에서 분리되어 시료주입이 완성되도록 하는 것을 특징으로 한다. 본 발명에 의하면, 시료 확산에 의한 신호의 손실을 최대한 막아주므로 검출 감도가 우수하고 높은 이론 단수를 제공하여 미량의 농도로 존재하는 물질 및 특히, 생명과학 연구에서 단세포 분석과 같은 극소부피의 세포내 물질의 검출을 포함한 다양한 시료 검출에 유용하게 적용할 수 있다. 마이크로 칩, 극미량 시료, 시료 분리