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公开(公告)号：KR101459721B1
公开(公告)日：2014-11-12
申请号：KR1020130007541
申请日：2013-01-23
Applicant: 한국과학기술연구원
IPC: C12Q1/68 , C12N15/113
CPC classification number: C12N15/1003 , C12Q1/6816 , C12Q2525/207 , C12Q2527/125
Abstract: 본 발명은 트리톤(Triton) X-100 첨가에 의한 세포 내 극소량 존재하는 miRNA 검출 효율을 증가시키는 방법에 관한 것이다. 본 발명에 따르면, 시료 내 극소량으로 존재하는 miRNA를 빠른 시간에 정량적으로 분석이 가능하다. 또한, 본 발명의 트리톤(Triton) X-100을 추가한 miRNA 검출방법은 트리졸 시약만을 이용하였을 경우와 비교하여 약 2배 이상 검출 효율을 증가시킬 수 있다.
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公开(公告)号：KR101880892B1
公开(公告)日：2018-07-24
申请号：KR1020160158170
申请日：2016-11-25
Applicant: 한국과학기술연구원
Abstract: 본발명은글리코겐및 효모유래 tRNA 첨가에의한혈장내 극소량존재하는 miRNA 검출효율을증가시키는방법에관한것이다. 본발명에따르면, 시료내 극소량으로존재하는 miRNA를빠른시간에정량적으로분석이가능하다. 또한, 본발명의글리코겐및 효모유래 tRNA를추가한 miRNA 검출방법은글리코켄(Glycogen) 단독, 효모유래 tRNA 단독으로첨가한경우와대비하여최소 3배이상높기때문에, 다양한 miRNA 검출하는데효율적으로활용될수 있다.
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公开(公告)号：KR1020140106061A
公开(公告)日：2014-09-03
申请号：KR1020130020062
申请日：2013-02-25
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6883 , C12Q2600/158 , C12Q2600/178 , C12N15/11 , G01N33/5005
Abstract: The present invention relates to a method for simultaneously detecting multiple miRNAs and a kit for detecting the same. According to the present invention, at least two miRNAs are analyzed via only one measuring, wherein a sample has a tiny amount of miRNAs. Also, the method of the present invention simultaneously detects multiple miRNAs related to diseases, leading to accurate diagnosis for cardiovascular diseases including myocardial infarction.
Abstract translation: 本发明涉及同时检测多种miRNA的方法和用于检测其的试剂盒。 根据本发明，通过仅一次测量分析至少两种miRNA，其中样品具有微量的miRNA。 此外，本发明的方法同时检测与疾病相关的多种miRNA，导致包括心肌梗塞在内的心血管疾病的准确诊断。
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公开(公告)号：KR101426530B1
公开(公告)日：2014-08-05
申请号：KR1020120110113
申请日：2012-10-04
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6883 , C12Q1/6816 , C12Q2600/158 , C12Q2600/178 , C12Q2525/207 , C12Q2565/629
Abstract: 본 발명은 시료 내 극소량으로 존재하는 miRNA를 검출하는 방법 및 이를 검출하기 위한 키트에 관한 것이다. 본 발명에 따르면, 시료 내 극소량으로 존재하는 miRNA를 빠른 시간에 정량적으로 분석이 가능하다. 또한, 본 발명의 검출방법은 miRNA를 활용한 다양한 질환의 진단, 예컨대 심근경색을 포함하는 심혈관 질환의 신속한 진단에 이용될 수 있다.
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Patent Agency Ranking
公开(公告)号：KR1020140044116A
公开(公告)日：2014-04-14
申请号：KR1020120110113
申请日：2012-10-04
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6883 , C12Q1/6816 , C12Q2600/158 , C12Q2600/178 , G01N2800/32 , C12Q2525/207 , C12Q2565/629
Abstract: The present invention relates to a detection method for a trace amount of miRNA present in a sample and a kit for detecting the same. According to the present invention, it is possible to quantitatively analyze a trace amount of miRNA present in the sample in a short period of time. In addition, the detection method of the present invention can be used in diagnosis of various diseases using miRNA, for example, in rapid diagnosis of cardiovascular diseases including myocardial infarction.
Abstract translation: 本发明涉及存在于样品中的微量miRNA的检测方法和用于检测样品的试剂盒。 根据本发明，可以在短时间内定量分析样品中痕量的miRNA。 此外，本发明的检测方法可用于诊断使用miRNA的各种疾病，例如用于心血管疾病（包括心肌梗塞）的快速诊断。
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16.发明授权
公开(公告)号：KR101351334B1
公开(公告)日：2014-01-22
申请号：KR1020110103796
申请日：2011-10-11
Applicant: 한국과학기술연구원
Abstract: 본원 발명은 ET-1에 의해 유도되는 심근비대증 관련 NFAT가 발현되는 것을 유도 형광 검출기(laser induce fluorecence)로 검출하고 모세관 전기 영동법을 이용하여 심근비대증을 확인, 판정 및 발병 기작을 규명하는데 효과적으로 사용될 수 있고 이를 통해 치료 약물의 개발이 가능하다.
또한 본 발명의 경우 심근 비대증을 판단하는 진단 키트 등을 통해 심근 비대증 환자에 대한발병 예방 및 차후 치료 약물의 개발에 유용하다.-
公开(公告)号：KR100451594B1
公开(公告)日：2004-10-08
申请号：KR1020020006554
申请日：2002-02-05
Applicant: 한국과학기술연구원
IPC: C12Q1/48
Abstract: PURPOSE: A determination method of protein phosphorylation reaction by enzyme activity is provided, thereby easily and rapidly analyzing the protein phosphorylation reaction. CONSTITUTION: A determination method of protein phosphorylation reaction by enzyme activity comprises the steps of: determining the optimal pH and concentration of a buffer solution for phosphorylation reaction of myelin basic protein(MBP) by extracellular signal-regulated kinase(ERK); adding the optimal concentration of compound to the buffer solution in order to inhibit the adsorption of peptide to the inner wall of a capillary tube and increase the detection sensitivity; determining MBP peptide from the active region of MBP peptide by ERK, -PRTP- as a center for effectively carrying out the phosphorylation reaction by ERK under the same condition; detecting the phosphorylation reaction of MBP peptide by ERK using a capillary tube electrophoresis using the buffer solution; and additionally confirming the phosphorylation reaction detected using a matrix-supported laser removing/ionizing mass analyzer.
Abstract translation: 目的：提供一种通过酶活性进行蛋白质磷酸化反应的测定方法，从而可以容易且快速地分析蛋白质磷酸化反应。 构成：通过酶活性确定蛋白质磷酸化反应的方法包括以下步骤：通过细胞外信号调节激酶（ERK）确定用于磷酸化髓磷脂碱性蛋白（MBP）反应的缓冲溶液的最佳pH和浓度; 将最佳浓度的化合物添加到缓冲液中以抑制肽吸附到毛细管内壁并提高检测灵敏度; 通过ERK以-PRTP-从MBP肽的活性区域测定MBP肽作为用于在相同条件下通过ERK有效进行磷酸化反应的中心; 使用缓冲溶液使用毛细管电泳检测由ERK检测MBP肽的磷酸化反应; 并且另外确认使用基质支持的激光去除/电离质量分析仪检测到的磷酸化反应。
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公开(公告)号：KR1020040065766A
公开(公告)日：2004-07-23
申请号：KR1020030002928
申请日：2003-01-16
Applicant: 한국과학기술연구원
IPC: G01N27/447
Abstract: PURPOSE: A method for analyzing a nitrite emitted from a cell by using a capillary electrophoresis is provided to reduce cost, time and labor for analyzing the reaction in a cell by using a capillary electrophoresis. CONSTITUTION: A method for analyzing a nitrite emitted from a cell by using a capillary electrophoresis comprises the steps of setting up a condition of a capillary electrophoresis, writing a concentration testing curve of a detecting peak versus a standard material by detecting a nitrite under the set condition, growing a cell on a medium, detecting a nitrite peak emitted from the cell by using a cell growing supernatant, and determining nitrite concentration by applying the nitrite peak to the concentration testing curve.
Abstract translation: 目的：提供一种通过使用毛细管电泳分析从细胞发出的亚硝酸盐的方法，以减少通过毛细管电泳分析细胞中的反应的成本，时间和劳动。 构成：通过使用毛细管电泳分析从细胞发出的亚硝酸盐的方法包括以下步骤：设置毛细管电泳的条件，通过检测所述组中的亚硝酸盐，将检测峰的浓度测试曲线与标准物质相比较 条件，在培养基上生长细胞，通过使用细胞生长上清液检测从细胞发出的亚硝酸盐峰，并通过将亚硝酸盐峰应用于浓度测试曲线来测定亚硝酸盐浓度。
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公开(公告)号：KR1020030066217A
公开(公告)日：2003-08-09
申请号：KR1020020006554
申请日：2002-02-05
Applicant: 한국과학기술연구원
IPC: C12Q1/48
Abstract: PURPOSE: A determination method of protein phosphorylation reaction by enzyme activity is provided, thereby easily and rapidly analyzing the protein phosphorylation reaction. CONSTITUTION: A determination method of protein phosphorylation reaction by enzyme activity comprises the steps of: determining the optimal pH and concentration of a buffer solution for phosphorylation reaction of myelin basic protein(MBP) by extracellular signal-regulated kinase(ERK); adding the optimal concentration of compound to the buffer solution in order to inhibit the adsorption of peptide to the inner wall of a capillary tube and increase the detection sensitivity; determining MBP peptide from the active region of MBP peptide by ERK, -PRTP- as a center for effectively carrying out the phosphorylation reaction by ERK under the same condition; detecting the phosphorylation reaction of MBP peptide by ERK using a capillary tube electrophoresis using the buffer solution; and additionally confirming the phosphorylation reaction detected using a matrix-supported laser removing/ionizing mass analyzer.
Abstract translation: 目的：提供通过酶活性测定蛋白质磷酸化反应的方法，从而容易且快速地分析蛋白质磷酸化反应。 构成：通过酶活性测定蛋白质磷酸化反应的方法包括以下步骤：通过细胞外信号调节激酶（ERK）测定髓磷脂碱性蛋白（MBP）的磷酸化反应缓冲溶液的最佳pH和浓度; 向缓冲溶液中加入最佳浓度的化合物，以抑制肽对毛细管内壁的吸附，提高检测灵敏度; 通过ERK，-PRTP-作为中心，通过ERK在相同条件下有效地进行磷酸化反应，从MBP肽的活性区确定MBP肽; 通过使用缓冲溶液的毛细管电泳检测ERK的MBP肽的磷酸化反应; 并且另外确认使用基质支持的激光去除/电离质量分析仪检测的磷酸化反应。
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