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公开(公告)号：KR1020140110550A
公开(公告)日：2014-09-17
申请号：KR1020130025111
申请日：2013-03-08
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6888 , C12Q1/6837 , C12Q2600/178 , G01N33/48
Abstract: The present invention relates to a methylation biomarker specific to human exposure to toluene, one of volatile organic compounds, and a detection method using the same and, more specifically, to a toluene exposure-specific marker gene having a promoter region of a certain gene specifically methylated by the exposure to toluene, and a method for identifying human exposure to toluene using the marker gene. The methylation marker of the present invention can be useful for accurately and rapidly monitoring and determining toluene contamination in human specimens exposed to environment compared to a conventional method, and also used as a tool identifying mechanisms of toxic action caused by toluene by using the methylated promoter region of a reactive gene selected by DNA methylation microarray chips as a biomarker.
Abstract translation: 本发明涉及对人体暴露于甲苯，挥发性有机化合物之一的甲基化生物标志物和使用其的检测方法，更具体地涉及具有特定基因的启动子区域的甲苯暴露特异性标记基因 通过暴露于甲苯进行甲基化，以及使用标记基因鉴定人类暴露于甲苯的方法。 与常规方法相比，本发明的甲基化标记可用于准确和快速地监测和测定暴露于环境中的人体样品中的甲苯污染物，并且还可用作通过使用甲基化启动子鉴定由甲苯引起的毒性作用机制的工具 通过DNA甲基化微阵列芯片作为生物标志物选择的反应性基因的区域。
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公开(公告)号：KR1020140110549A
公开(公告)日：2014-09-17
申请号：KR1020130025109
申请日：2013-03-08
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6888 , C12Q1/6837 , C12Q2600/178
Abstract: The present invention relates to microRNA for identifying xylene exposure in a human body, and a method for identifying xylene exposure using the same. More particularly, the present invention can be usefully used for monitoring and deciding pollution by xylene in a human sample exposed to environment by using the microRNA whereby an expression is specifically increased or decreased via xylene as a biomarker. Moreover, the present invention can also be used as a tool investigating a mechanism of toxic effect caused by xylene.
Abstract translation: 本发明涉及用于鉴定人体二甲苯暴露的微小RNA，以及使用该微RNA识别二甲苯曝光的方法。 更具体地说，本发明可用于通过使用微小RNA来监测和确定暴露于环境的人体样品中的二甲苯的污染，从而通过作为生物标志物的二甲苯具体地增加或减少表达。 此外，本发明也可以用作研究由二甲苯引起的毒性作用机理的工具。
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公开(公告)号：KR1020130134152A
公开(公告)日：2013-12-10
申请号：KR1020120057452
申请日：2012-05-30
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6837 , C12Q1/686 , C12Q1/6876 , C12Q1/6883 , C12Q2600/142 , C12Q2600/158 , C12Q2600/178
Abstract: The present invention relates to microRNAs for identifying exposure to lower aliphatic saturated aldehydes and an identification method using the same. More specifically, the present invention selects one kind of microRNA which is overexpressed until expression level becomes 1.5 times or more due to exposure to the lower aliphatic saturated aldehydes and one kind of microRNA which is lessexpressed until expression level becomes 0.66 times or less and uses two kinds of the microRNAs as biomarkers in order to monitor and judge risk of the lower aliphatic saturated aldehydes and as a tool for investigating mechanism of toxic activities caused by the lower aliphatic saturated aldehydes. [Reference numerals] (AA,KK) MicroRNA expression (Fold Change);(BB,LL) Microarray;(CC,MM) Real time RT-PCR;(DD,NN) Propionaldehyde;(EE,OO) Butylaldehyde;(FF,PP) Valeraldehyde;(GG,QQ) Hexanal;(HH,RR) Heptanal;(II,SS) Octanal;(JJ,TT) Nonanal
Abstract translation: 本发明涉及用于鉴定暴露于低级脂族饱和醛的微小RNA及其鉴定方法。 更具体地说，本发明选择一种过表达的微小RNA，由于暴露于低级脂肪族饱和醛和低表达水平的一种微小RNA直到表达水平变为0.66倍以下，并且使用两种 微RNA的种类为生物标志物，以监测和判断低级脂肪族饱和醛的风险，并作为研究由低级脂族饱和醛引起的毒性活性机理的工具。 参考数字（AA，KK）微RNA表达（折叠变化）;（BB，LL）微阵列;（CC，MM）实时RT-PCR;（DD，NN）丙醛;（EE，OO）丁醛;（FF ，PP）戊醛;（GG，QQ）己醛;（HH，RR）庚醛;（II，SS）辛醛;（JJ，TT）壬醛
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公开(公告)号：KR1020130133456A
公开(公告)日：2013-12-09
申请号：KR1020120056716
申请日：2012-05-29
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6876 , C12Q1/6883 , C12Q2600/142 , C12Q2600/158 , C12Q1/6837
Abstract: The present invention relates to a biomarker for identifying specific exposure to lower aliphatic saturated aldehydes, one of volatile organic compounds exposed in the environment, and a method for identification using the same and, more specifically, a biomarker of which the gene expression is up- or down-regulated specifically by lower aliphatic saturated aldehydes and a method for identifying specific exposure to lower aliphatic saturated aldehydes using the biomarker. The biomarker in the present invention can be useful for monitoring or evaluating lower aliphatic saturated aldehyde contamination in the environmental samples using genes selected by DNA microarray chip as the biomarkers and be used as a tool for investigating the toxic mechanism specifically induced by the lower aliphatic saturated aldehydes. [Reference numerals] (A1,A2,A3,A4,A5) Gene expression rate against control group;(B1,C1,D1,E1,F1) Propionaldehyde;(B2,C2,D2,E2,F2) Butylaldehyde;(B3,C3,D3,E3,F3) Valeraldehyde;(B4,C4,D4,E4,F4) Hexanal;(B5,C5,D5,E5,F5) Heptanal;(B6,C6,D6,E6,F6) Octanal;(GG) Microarray;(HH) Real time RT-PCR
Abstract translation: 本发明涉及一种用于鉴定特定暴露于低级脂族饱和醛（一种在环境中暴露的挥发性有机化合物）的生物标志物，以及使用其的鉴定方法，更具体地说，涉及基因表达上调的生物标志物， 或通过低级脂族饱和醛特异性下调，以及使用该生物标志物鉴定特定暴露于低级脂肪族饱和醛的方法。 本发明的生物标志物可用于利用DNA微阵列芯片选择的基因作为生物标志物监测或评价环境样品中的低级脂肪族饱和醛污染物，并用作研究由低级脂族饱和物特异性诱导的毒性机制的工具 醛。 （A1，A2，A3，A4，A5）对照组的基因表达率;（B1，C1，D1，E1，F1）丙醛;（B2，C2，D2，E2，F2）丁醛;（B3 （B6，C6，D6，E6，F6）辛醛;（B5，C5，D5，E5，F5）庚醛;（B6，C6，D6，E6，F6） （GG）微阵列;（HH）实时RT-PCR
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15.发明授权갑상선 과산화효소 활성 증가 화학물질 노출 여부 확인용 바이오마커 및 이를 이용한 확인방법 有权用于鉴定暴露于激活甲状腺过氧化物酶的破坏剂的生物标志物和使用相同生物标志物激活甲状腺过氧化物酶暴露的破坏剂的鉴定方法
公开(公告)号：KR101138953B1
公开(公告)日：2012-04-25
申请号：KR1020100021748
申请日：2010-03-11
Applicant: 한국과학기술연구원
Abstract: 본 발명은 갑상선 과산화효소의 활성을 증진시키는 화학물질에 대한 노출 여부 확인용 DNA 마이크로어레이 칩 및 상기 마이크로어레이 칩을 이용하여 갑상선 과산화효소의 활성을 증진시키는 화학물질에 대한 노출 여부를 확인하는 방법에 관한 것으로, 구체적으로는 갑상선 호르몬 합성에 중요한 역할을 하는 갑상선 과산화효소를 활성화하는 화학물질인 카바릴 또는 디부틸프탈레이트에 대한 노출 여부 확인용 바이오마커, 상기 바이오마커가 집적된 DNA 마이크로어레이 칩 및 이를 이용한 확인 방법에 관한 것으로 본 발명의 바이오마커는 DNA 마이크로어레이를 통하여 선별된 페리틴(ferritin) 관련 유전자들을 바이오마커로 이용하여 환경 시료에서 갑상선 과산화효소를 활성화하는 화학물질의 오염을 모니터링 및 판정하는데 유용하게 사용될 수 있으며, 갑상선 과산화효소를 활성화하는 화학물질에 의해 유발되는 독성 작용 기작을 규명하는 도구로 이용될 수 있다.
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Patent Agency Ranking
公开(公告)号：KR1020110092857A
公开(公告)日：2011-08-18
申请号：KR1020100012523
申请日：2010-02-10
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6888 , C12Q1/6837 , G01N33/5067
Abstract: PURPOSE: A method for detecting mirex-specific exposure using a biomarker is provided to monitor mirex and to determine risk of mirex. CONSTITUTION: A method for detecting mirex-specific exposure using a biomarker comprises: a step of isolating RNA from human somatic cells of a test sample as an experimental group and from somatic cells of a control group; a step of labeling the experimental group and control group with different fluorescent substrates; a step of hybridizing the cDNA on a DNA microarray chip; and a step of analyzing the DNA microarray chip.
Abstract translation: 目的：提供使用生物标志物检测灭蚁灵特异性暴露的方法来监测灭蚁灵并确定灭蚁灵的风险。 构成：使用生物标志物检测灭蚁灵特异性暴露的方法包括：将作为实验组的测试样品的人体细胞的RNA和对照组的体细胞分离的步骤; 用不同荧光底物标记实验组和对照组的步骤; 将cDNA杂交到DNA微阵列芯片上的步骤; 以及分析DNA微阵列芯片的步骤。
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公开(公告)号：KR100978791B1
公开(公告)日：2010-08-31
申请号：KR1020080006572
申请日：2008-01-22
Applicant: 한국과학기술연구원
Inventor： 류재천 , 김연정 , 송미 , 샤르마사이렌드라나쓰
Abstract: 본 발명은 휘발성 유기화합물 중의 하나인 디클로로메탄(dichloromethane)에 대한 노출 여부 확인용 바이오마커 및 이를 이용한 확인 방법에 관한 것으로, 구체적으로 디클로로메탄에 의해 특이적으로 유전자 발현이 증가 또는 감소하는 바이오마커 및 이를 이용한 디클로로메탄에 대한 노출 여부를 확인하는 방법에 관한 것이다. 본 발명의 바이오마커는 DNA 마이크로어레이 칩을 통하여 선별된 반응 유전자들을 바이오마커로 이용하여 환경 시료에서 디클로로메탄의 오염을 모니터링 및 판정하는데 유용하게 사용될 수 있으며, 디클로로메탄에 의해 유발되는 독성 작용 기작을 규명하는 도구로 이용될 수 있다.
디클로로메탄, 휘발성 유기화합물, 바이오마커, 마이크로 어레이-
公开(公告)号：KR1020090071808A
公开(公告)日：2009-07-02
申请号：KR1020070139711
申请日：2007-12-28
Applicant: 한국과학기술연구원
CPC classification number: C40B40/06 , C12Q1/6837 , C12Q2561/113 , C12Q2563/107
Abstract: A marker gene for screening a drug which induces pulmonary toxicity is provided to monitor and determine a drug or chemical substance causing pulmonary toxicity by increasing or decreasing the expression of the marker by nitofurantoin. An expression of marker gene for screening a drug which causes pulmonary toxicity is increased or decreased by the drug treatment. The drug causing pulmonary toxicity is nitrofurantoin. A DNA microarray chip for screening the drug comprises oligonucleotide containing whole or a part of marker gene sequence or their complement strand. A method for screening the drug comprises: a step of treating a subject compound in a human bronchiolar cell or lung cell; a step of isolating RNA from the subject compound-treated experimental group and untreated-control group; a step of synthesizing cDNA and labeling with each different fluorescence; a step of hybridizing cDNA with DNA microarray; a step of analyzing DNA microarray; and a step of comparing the expressions between the experimental group and control group.
Abstract translation: 提供用于筛选诱导肺毒性的药物的标记基因，通过增加或减少由硝呋喃素标记物的表达来监测和测定引起肺部毒性的药物或化学物质。 用于筛选引起肺毒性的药物的标记基因的表达通过药物治疗而增加或减少。 导致肺部毒性的药物是呋喃妥因。 用于筛选药物的DNA微阵列芯片包含含有全部或部分标记基因序列或其互补链的寡核苷酸。 筛选药物的方法包括：处理人支气管细胞或肺细胞中的受试化合物的步骤; 从受试化合物处理的实验组和未处理对照组中分离RNA的步骤; 合成cDNA并用各种不同荧光标记的步骤; 将cDNA与DNA微阵列杂交的步骤; 分析DNA微阵列的一个步骤; 并比较实验组与对照组之间的表达。
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19.发明公开독소루비신 처리에 따른, 심장독성 유발 약물 검색용마커유전자 및 이를 이용한 심장독성 유발 약물 검색 방법 失效基于DOXORUBICIN治疗的药物基因，用于筛选药物诱导致病毒性及其筛选方法
公开(公告)号：KR1020080112764A
公开(公告)日：2008-12-26
申请号：KR1020070061573
申请日：2007-06-22
Applicant: 한국과학기술연구원
IPC: C12Q1/68
CPC classification number: C12Q1/6837 , G01N33/5008
Abstract: Marker genes for screening drug inducing cardiotoxicity and a screening method thereof are provided to monitor and determine drugs or chemical substances having the risk of heart toxicity by using the reaction genes selected through a DNA microarray chip. A screening method drug inducing cardiotoxicity comprises (1) a step for processing an object compound in HUVEC; (2) a step for separating RNA from the experimental group cell which processes the object compound of the step (1) and the control cell which does not process the object compound; (3) a step for respectively making the experimental group and control group by different fluorescent materials while synthesizing RNA of the experimental group of the step (2) and the control group to cDNA; (4) a step for mixing the cDNA respectively marked by different fluorescent materials of the step (3) with the DNA microarray; (5) a step for analyzing the DNA reacted microarray of the step (4); and (6) a step for comparing and confirming the expressed grade of the marker gene with the control group.
Abstract translation: 提供用于筛选药物诱导心脏毒性的标记基因及其筛选方法，以通过使用通过DNA微阵列芯片选择的反应基因来监测和测定具有心脏毒性风险的药物或化学物质。 筛选方法药物诱导心脏毒性包括（1）在HUVEC中加工目标化合物的步骤; （2）分离来自处理步骤（1）的目标化合物和不处理目标化合物的对照细胞的实验组细胞的RNA的步骤; （3）步骤（2）和对照组合成RNA，分别使不同荧光物质分别制作实验组和对照组; （4）将由步骤（3）的不同荧光材料分别标记的cDNA与DNA微阵列混合的步骤; （5）分析步骤（4）的DNA反应的微阵列的步骤; 和（6）比较和确认标记基因与对照组的表达等级的步骤。
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公开(公告)号：KR101460529B1
公开(公告)日：2014-11-10
申请号：KR1020130025111
申请日：2013-03-08
Applicant: 한국과학기술연구원
Abstract: 본 발명은 휘발성 유기 화합물류 중의 하나인 톨루엔(Toluene)의 인체 노출에 특이적인 메틸화 바이오마커 및 이를 이용한 검출방법에 관한 것으로, 구체적으로 톨루엔에 의해 특정 유전자의 프로모터 부위가 특이적으로 메틸화되는 톨루엔 노출 특이적 마커 유전자 및 이를 이용한 톨루엔에 대한 인체 노출 확인 방법에 관한 것이다. 본 발명의 메틸화 마커는 DNA 메틸화 마이크로어레이 칩을 통하여 선별된 반응 유전자의 메틸화된 프로모터 부위를 바이오마커로 이용하여 통상적인 방법보다 정확하고 빠르게 환경 중 노출된 인체 시료에서 톨루엔의 오염을 모니터링 및 판정하는데 유용하게 사용될 수 있으며, 톨루엔에 의해 유발되는 독성 작용 기작을 규명하는 도구로 이용될 수 있다.
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