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公开(公告)号：KR101383653B1
公开(公告)日：2014-04-10
申请号：KR1020120040407
申请日：2012-04-18
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6813 , C12Q1/686 , C12Q1/6883 , C12Q2600/142 , C12Q2600/158
Abstract: 본 발명은 환경 중 노출되는 휘발성 유기화합물 중의 하나인 프로피온알데히드(Propionaldehyde) 특이적 노출 여부 확인용 바이오마커 및 이를 이용한 확인 방법에 관한 것으로, 구체적으로 프로피온알데히드에 의해 특이적으로 유전자 발현이 증가 또는 감소하는 바이오마커 및 이를 이용한 프로피온알데히드에 특이적 노출 여부를 확인하는 방법에 관한 것이며, 본 발명의 바이오마커는 DNA 마이크로어레이 칩을 통하여 선별된 반응 유전자들을 바이오마커로 이용하여 환경 시료에서 프로피온알데히드의 오염을 모니터링 및 판정하는데 유용하게 사용될 수 있으며, 프로피온알데히드에 의해 특이적으로 유발되는 독성 작용 기작을 규명하는 도구로 이용될 수 있다.
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公开(公告)号：KR1020130117298A
公开(公告)日：2013-10-25
申请号：KR1020120040407
申请日：2012-04-18
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6813 , C12Q1/686 , C12Q1/6883 , C12Q2600/142 , C12Q2600/158 , C12Q1/6837
Abstract: PURPOSE: A biomarker for identifying propionaldehyde-specific exposure and an identification method using the same are provided to monitor propionaldehyde and to determine risks using a response gene selected by a DNA microarray chip. CONSTITUTION: A DNA microarray chip for identifying propionaldehyde-specific exposure contains nucleic acid sequences of one or more genes selected among angiotensinogen (AGT) gene (Genbank accession number NM_0000299), lysophosphatidic acid receptor 1 (LPAR1) gene (Genbank accession number NM_057159), secreted and transmembrane 1 (SECTM1, Genbank accession number NM_003004), tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10) gene (Genbank accession number NM_003810), and heme oxygenase 1 (HMOX1) gene (Genbank accession number NM_002133) or a complementary strand thereof. A method for identifying propionaldehyde exposure comprises the steps of: isolating RNAs from somatic cells of an experimental group and a normal control group; synthesizing cDNAs from the RNAs; labeling the cDNAs with different fluorescent materials; hybridizing the cDNAs onto a DNA microarray chip; analyzing the DNA microarray chip; and comparing the expression levels of genes of the control group and the experimental group, integrated on the DNA microarray chip.
Abstract translation: 目的：提供用于鉴定丙醛特异性暴露的生物标志物和使用其的鉴定方法以监测丙醛并使用由DNA微阵列芯片选择的响应基因来确定风险。 构成：用于鉴定丙醛特异性暴露的DNA微阵列芯片含有选自血管紧张素原（AGT）基因（Genbank登录号NM_0000299），溶血磷脂酸受体1（LPAR1）基因（Genbank登录号NM_057159）中的一种或多种基因的核酸序列， 分泌和跨膜1（SECTM1，Genbank登录号NM_003004），肿瘤坏死因子（配体）超家族，成员10（TNFSF10）基因（Genbank登录号NM_003810）和血红素加氧酶1（HMOX1）基因（Genbank登录号NM_002133）或 互补链。 用于鉴定丙醛暴露的方法包括以下步骤：从实验组和正常对照组的体细胞中分离RNA; 从RNA合成cDNA; 用不同荧光材料标记cDNAs; 将cDNA杂交到DNA微阵列芯片上; 分析DNA微阵列芯片; 并比较对照组和实验组的基因表达水平，整合到DNA微阵列芯片上。
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公开(公告)号：KR1020130107552A
公开(公告)日：2013-10-02
申请号：KR1020120029432
申请日：2012-03-22
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/6883 , C12Q1/6837 , C12Q2600/158
Abstract: PURPOSE: A biomarker for detecting butylaldehyde-specific exposure is provided to monitor and determine butylaldehyde contamination in an environmental sample using response genes selected by a DNA microarray chip. CONSTITUTION: A DNA microarray chip containing integrated nucleic acid sequences of a gene or molecules of complementary strands thereof is used for detecting butylaldehyde-specific exposure. The gene has gene registration number of Genebank NM_022740 (HIPK2, homeodomain interacting protein kinase 2). A method for detecting butylaldehyde exposure comprises the steps of: isolating each RNA from somatic cells of an experimental group which is suspected to be exposed by butylaldehyde and a control group; synthesizing cDNAs of the experimental group and the control group from the RNAs; labeling each cDNA with different fluorescent materials; hybridizing the labeled cDNAs onto the DNA microarray chip; analyzing the reacted DNA microarray chip; and comparing the expression levels of genes which are integrated on the DNA microarray chip between the experimental group and the control group.
Abstract translation: 目的：提供用于检测丁醛特异性暴露的生物标志物，以使用由DNA微阵列芯片选择的应答基因来监测和测定环境样品中的丁醛污染。 构成：使用含有其互补链的基因或其分子的整合核酸序列的DNA微阵列芯片来检测丁醛特异性暴露。 该基因具有Genebank NM_022740（HIPK2，同源结构域相互作用蛋白激酶2）的基因登录号。 用于检测丁醛暴露的方法包括以下步骤：从怀疑被丁醛暴露的实验组和对照组的体细胞中分离每个RNA; 合成来自RNA的实验组和对照组的cDNA; 用不同荧光材料标记每个cDNA; 将标记的cDNA杂交到DNA微阵列芯片上; 分析反应的DNA微阵列芯片; 并比较实验组和对照组之间的DNA微阵列芯片上整合的基因的表达水平。
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