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11.发明授权머리카락 내 ２４Ｓ－히드록시콜레스테롤의 검출방법과생체 대사산물 배열에 의한 치매 대사 표지자의 평가방법 有权检测头发中的24S-羟基胆固醇，并用代谢物阵列评估阿尔茨海默病的代谢生物标志物
公开(公告)号：KR101026527B1
公开(公告)日：2011-04-01
申请号：KR1020080049727
申请日：2008-05-28
Applicant: 한국과학기술연구원
Abstract: 본 발명은 머리카락 내 24S-히드록시콜레스테롤의 검출방법과 생체 대사산물의 배열에 의한 치매 대사표시자의 평가방법에 관한 것으로서, 더욱 상세하게는 소량의 머리카락을 염기성 조건에서 가수분해한 후, 고체상 추출법으로 24S-히드록시콜레스테롤을 포함한 생체 대사산물들을 정제 및 추출하고, 트리메틸실릴(trimethylsilyl, TMS) 유도체 과정을 거쳐 기체 크로마토그래피-질량분석기로 분석하여 24S-히드록시콜레스테롤의 농도를 측정하여 24S-히드록시콜레스테롤을 검출하는 방법에 관한 것이다.
또한, 머리카락 내에 존재하는 대사산물의 분포경향을 비교하기 위하여 대사산물의 배열에 의한 치매 대사표지자의 평가방법도 포함한다.
따라서, 본 발명은 소량의 머리카락으로부터 24S-히드록시콜레스테롤의 농도와 생체 대사산물들의 분포양상을 비교하여 치매 유무를 평가할 수 있는 기술을 제공할 수 있다.
머리카락, 24S-히드록시콜레스테롤, 검출방법, 대사표지자, 치매-
公开(公告)号：KR100785913B1
公开(公告)日：2007-12-17
申请号：KR1020060119300
申请日：2006-11-29
Applicant: 한국과학기술연구원
CPC classification number: C08F251/00 , C08B37/0012 , C08L5/16 , C08L51/02 , G01N33/548 , G01N33/743 , G01N2600/00 , C08L2666/02
Abstract: A cured beta-cyclodextrin polymer powder, its preparation method, and a filler for a solid phase extraction containing the cured beta-cyclodextrin polymer powder are provided to extract a steroid hormone present in a living body sample simply and effectively by using the cured beta-cyclodextrin polymer powder. A cured beta-cyclodextrin polymer powder comprises a beta-cyclodextrin polymer prepared by the reaction of a beta-cyclodextrin monomer and epichlorohydrin; and a metal ion which is coated on the surface of the beta-cyclodextrin polymer. Preferably the metal ion is selected from Ca and Na. The cured beta-cyclodextrin polymer powder is prepared by dissolving beta-cyclodextrin in an alkaline aqueous solution and adding epichlorohydrin to it to prepare a beta-cyclodextrin polymer of gel state; dipping the polymer in a metal salt aqueous solution to coat the polymer with a metal ion, thereby preparing a cured beta-cyclodextrin polymer; and washing, drying and pulverizing the obtained polymer.
Abstract translation: 提供固化的β-环糊精聚合物粉末，其制备方法和用于固相β-环糊精聚合物粉末的固相提取填料，通过使用固化的β-环糊精聚合物粉末提取活体样品中存在的类固醇激素， 环糊精聚合物粉末。 固化的β-环糊精聚合物粉末包含通过β-环糊精单体和表氯醇反应制备的β-环糊精聚合物; 以及涂覆在β-环糊精聚合物表面上的金属离子。 优选地，金属离子选自Ca和Na。 固化的β-环糊精聚合物粉末通过将β-环糊精溶解在碱性水溶液中并加入表氯醇来制备凝胶状态的β-环糊精聚合物来制备; 将聚合物浸渍在金属盐水溶液中以用金属离子涂覆聚合物，从而制备固化的β-环糊精聚合物; 并洗涤，干燥和粉碎所得聚合物。
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公开(公告)号：KR101426529B1
公开(公告)日：2014-08-05
申请号：KR1020120121162
申请日：2012-10-30
Applicant: 한국과학기술연구원
Abstract: 본 발명은 머리카락 내 콜레스테롤 화합물의 농도 또는 콜레스테롤 화합물의 대사 효소 활성에 대한 계절별 변화를 측정하는 방법 및 이를 활용한 콜레스테롤 관련 질환의 유무, 발병 가능성 또는 호전의 정도를 예측하는 방법에 관한 것이다. 본 발명에 따르면, 한 올의 머리카락만으로도 인체 내 콜레스테롤 화합물 농도의 계절별 변화를 매우 정확히 측정하여 기존의 주기적인 혈액 검사보다 안전하고 손쉬운 방법으로 측정할 수 있다. 또한, 본 발명은 기존의 혈액 시료 채취의 어려움을 보완하고, 실질적으로 건강검진에 적용하여 보다 쉽게 콜레스테롤 대사 변화와 관련되는 질환들을 예측할 수 있는 장점을 갖는다.
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公开(公告)号：KR1020140054985A
公开(公告)日：2014-05-09
申请号：KR1020120121162
申请日：2012-10-30
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/60 , C12Q1/26 , C12Y103/01021 , C12Y103/01072 , C12Y114/13017 , C12Y114/15006 , G01N30/7206
Abstract: The present invention relates to a method for measuring seasonal changes in the concentration of a cholesterol compound in hair or the metabolic enzyme activity of a cholesterol compound and to a method for predicting the occurrence of cholesterol-related diseases, the possibilities of occurrence, or the degree of alleviation. According to the present invention, a seasonal change in the concentration of a cholesterol compound in the human body with just one strand of hair can be very accurately measured so that measurement can be performed more stably and easily than in existing periodical blood tests. Moreover, the method of the present invention reinforces existing difficulties in obtaining blood samples and is effectively applied to checkups so that disease related to cholesterol metabolism can be easily predicted.
Abstract translation: 本发明涉及一种测定头发中胆固醇化合物浓度的季节性变化或胆固醇化合物的代谢酶活性的方法，以及预测胆固醇相关疾病发生的方法，发生的可能性，或 减轻程度。 根据本发明，可以非常精确地测量人体中仅含有一根毛发的胆固醇化合物浓度的季节性变化，从而可以比现有的定期血液试验更稳定和容易地进行测量。 此外，本发明的方法加强了获得血液样本的现有困难，并且有效地应用于检查，从而可以容易地预测与胆固醇代谢相关的疾病。
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公开(公告)号：KR101152902B1
公开(公告)日：2012-06-07
申请号：KR1020100096995
申请日：2010-10-05
Applicant: 한국과학기술연구원
Abstract: 본발명은측정된머리카락내 스테로이드호르몬들의농도를통계적인방법으로처리하여각 스테로이드호르몬의농도뿐만아니라그들의대사에관여하는효소들의활성을동시에측정, 비교할수 있는분석방법에관한것이다. 본발명은소량의머리카락으로부터스테로이드호르몬들을효과적으로추출및 분석은물론대사에관여하는스테로이드간의비율을나타냄으로서총체적인대사분포경향을파악할수 있다. 따라서, 스테로이드호르몬대사와관련있는내분비질환및 생물학적변화를평가할수 있는방법으로사용될수 있으며, 특히탈모관련질환에대하여그 응용범위를확장할수 있다.
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Patent Agency Ranking
公开(公告)号：KR101152655B1
公开(公告)日：2012-06-05
申请号：KR1020100058131
申请日：2010-06-18
Applicant: 한국과학기술연구원
Abstract: 본발명은머리카락을염기성조건에서가수분해시킨후, 트리에틸아민완충용액을전개용매로하여분석방해물질을제거하고분석물질을농축시킨다음, 트리에틸아민용액과아세토니트릴을전개용매로공급하고분석물질을용출시켜액체크로마토그래피-질량분석법으로머리카락내 DHEA 설페이트및 콜레스테롤설페이트의농도를동시에측정하고, 여드름질환의정도에따라차별화되는머리카락내 DHEA 설페이트와콜레스테롤설페이트의농도를비교분석하는방법에관한것이다. 따라서본 발명은머리카락을사용한여드름질환의평가방법으로응용될수 있으리라기대된다.
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公开(公告)号：KR1020110138042A
公开(公告)日：2011-12-26
申请号：KR1020100058131
申请日：2010-06-18
Applicant: 한국과학기술연구원
Abstract: PURPOSE: A method for evaluating acne based on the detection of dehydroepiandrosterone(DHEA)-sulfate and cholesterol sulfate in hair is provided to confirm the concentration difference of the DHEA-sulfate and the cholesterol sulfate in a normal sample and a sample from an acne patient. CONSTITUTION: Hair is hydrolyzed under a basic condition. A triethylamine buffer solution is used as a developing solvent to eliminate analysis interfering substance from the hair. An analyzing substance is concentrated using a concentrated column. A triethylamine solution and acetonitrile is supplied to the analyzing substance as a developing solvent to obtain the analyzing substance from the concentrated column. The eluted analyzing substance is applied to an analyzing column. The concentrations of DHEA sulfate and cholesterol sulfate are measured using a liquid chromatographic-mass spectrometry.
Abstract translation: 目的：提供一种基于头发脱氢表雄酮（DHEA） - 硫酸盐和胆固醇硫酸盐的检测来评估痤疮的方法，以确定正常样品和痤疮患者样品中DHEA-硫酸盐和胆固醇硫酸盐的浓度差异 。 构成：毛发在基本条件下水解。 使用三乙胺缓冲溶液作为显影溶剂，以消除头发中的干扰物质的分析。 使用浓缩柱浓缩分析物质。 将三乙胺溶液和乙腈作为展开剂供给到分析物质中，从浓缩塔中得到分析物质。 将洗脱分析物质应用于分析柱。 使用液相色谱 - 质谱法测定DHEA硫酸盐和胆固醇硫酸盐的浓度。
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公开(公告)号：KR1020100095729A
公开(公告)日：2010-09-01
申请号：KR1020090014690
申请日：2009-02-23
Applicant: 한국과학기술연구원
IPC: G01N33/74 , G01N33/573 , G01N30/74
Abstract: PURPOSE: A method for analyzing difference of steroid hormone bio metabolite from two sample groups is provided to general metabolism tendency. CONSTITUTION: A method for analyzing difference of steroid hormone bio metabolite from two sample groups comprises: a step of extracting steroid hormone bio metabolite including male hormone, female hormone, corpus luteum hormone, sterol, and adrenal cortical hormone; a step of extracting the extracts again using an organic solvent; a step of measuring concentration of steroid hormone bio metabolite in urine by gas chromatography-mass spectrometry; and a step of comparing the concentration of liver steroid hormone bio metabolite between two samples. The organic solvent is a mixture solvent containing ethyl acetate and normal hexane.
Abstract translation: 目的：分析两种样品组类固醇激素生物代谢产物差异的一般方法。 构成：从两个样本组分析类固醇激素生物代谢物差异的方法包括：提取类固醇激素生物代谢物，包括雄性激素，雌激素，黄体激素，固醇和肾上腺皮质激素的步骤; 使用有机溶剂再次提取提取物的步骤; 通过气相色谱 - 质谱法测定尿液中类固醇激素生物代谢物的浓度的步骤; 并比较两个样品之间肝脏类固醇激素生物代谢物的浓度。 有机溶剂是含乙酸乙酯和正己烷的混合溶剂。
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公开(公告)号：KR100817934B1
公开(公告)日：2008-03-31
申请号：KR1020060119299
申请日：2006-11-29
Applicant: 한국과학기술연구원
IPC: C07J1/00
CPC classification number: C07J75/00
Abstract: A selective extraction method of a steroid compound using polymerized beta-cyclodextrin is provided to extract various shapes of the steroid hormones from a biological sample effectively through a simple process. A selective extraction method of a steroid compound using polymerized beta-cyclodextrin powder comprises the steps of: adding buffer solution and hydrolase to a liquid sample including the steroid compound; removing a water layer by centrifugation after adding and stirring hardened beta-cyclodextrin powder that beta-cyclodextrin monomer forms a layer structure by being bridged-bonded by epichlorohydrin and metal ions are spread between the layers of the bridged beta-cyclodextrin polymer to the reaction solution; and extracting the steroid compound by applying organic solvents after adding phosphate buffer solution and polar organic solvents, and controlling pH at a range of 9.0 - 10.0.
Abstract translation: 提供使用聚合β-环糊精的类固醇化合物的选择性提取方法，通过简单的方法有效地从生物样品中提取各种形状的类固醇激素。 使用聚合的β-环糊精粉末的类固醇化合物的选择性提取方法包括以下步骤：向包含类固醇化合物的液体样品中加入缓冲溶液和水解酶; 通过在加入和搅拌硬化的β-环糊精粉末之后通过离心除去水层，β-环糊精单体通过用表氯醇和金属离子桥接键合形成层结构，在桥连的β-环糊精聚合物层之间铺展到反应溶液 ; 并在加入磷酸盐缓冲液和极性有机溶剂后通过施加有机溶剂萃取类固醇化合物，并控制pH在9.0 - 10.0的范围内。
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