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Patent Agency Ranking11.发明公开
公开(公告)号：KR1020080063592A
公开(公告)日：2008-07-07
申请号：KR1020070000177
申请日：2007-01-02
Applicant: 한국과학기술원
Abstract: A recombinant microorganism is provided to secret a highly concentrated human epidermal growth factor in a pure state into a cell culture solution, thereby isolating and purifying the human epidermal growth factor very efficiently. A recombinant microorganism is co-transformed by a recombinant vector including a gene encoding an outer membrane protein F(OmpF) derived from E. coli and a recombinant vector including a gene encoding a human epidermal growth factor and secreting and expressing the human epidermal growth factor into a periplast of microorganism, thereby the gene encoding the OmpF and the gene encoding the human epidermal growth factor being expressed simultaneously and the human epidermal growth factor being extracellularly produced. A method for extracellular production of the human epidermal growth factor comprises the steps of: (a) culturing the recombinant microorganism to secret the human epidermal growth factor into a culture solution; and (b) collecting the human epidermal growth factor from the culture solution.
Abstract translation: 提供重组微生物以将纯的高度浓缩的人表皮生长因子分离成细胞培养液，从而非常有效地分离和纯化人表皮生长因子。 重组微生物通过包含编码来自大肠杆菌的外膜蛋白F（OmpF）的基因的重组载体和包含编码人表皮生长因子的基因的重组载体共转化，并分泌并表达人表皮生长因子 成为微生物的周质，从而编码OmpF的基因和编码人表皮生长因子的基因同时表达，并且人表皮生长因子被细胞外产生。 人表皮生长因子的细胞外产生方法包括以下步骤：（a）培养重组微生物以将人表皮生长因子分解成培养液; 和（b）从培养液中收集人表皮生长因子。
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公开(公告)号：KR101231897B1
公开(公告)日：2013-02-08
申请号：KR1020100075066
申请日：2010-08-03
Applicant: 한국과학기술원
CPC classification number: C12N15/70 , C12N9/001 , C12N9/1029 , C12N9/104 , C12N9/1085 , C12N9/1096 , C12N9/88 , C12N9/93 , C12N15/52 , C12P13/001 , C12Y103/01026 , C12Y203/01057 , C12Y205/01016 , C12Y206/01082 , C12Y401/0102 , C12Y402/01051 , C12Y403/03007
Abstract: 본 발명은 카다베린 고생성능을 가지는 변이 미생물 및 이를 이용한 카다베린의 제조방법에 관한 것으로, 보다 구체적으로는 카다베린(cadaverine)의 분해 또는 이용 경로에 관여하는 유전자가 약화 또는 결실되어 있는 카다베린 고생성능을 가지는 변이 미생물 및 이를 호기조건하에서 배양하여 퓨트레신을 고수율로 제조하는 방법에 관한 것이다. 본 발명에 따른 카다베린 고생성능을 가지는 변이 미생물은 산업적으로 널리 사용되는 카다베린을 고수율로 제조하는데 유용하다.
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公开(公告)号：KR101188432B1
公开(公告)日：2012-10-08
申请号：KR1020090018781
申请日：2009-03-05
Applicant: 한국과학기술원
Abstract: 본 발명은 퓨트레신 생성 대사경로를 가지는 미생물에서, 퓨트레신(putrescine)의 분해 또는 이용 경로에 관여하는 유전자가 약화 또는 결실되어 있는 퓨트레신 고생성능을 가지는 변이 미생물 및 이를 혐기조건하에서 배양하여 퓨트레신을 고수율로 제조하는 방법에 관한 것이다. 본 발명에 따른 퓨트레신(putrescine) 고생성능을 가지는 변이 미생물은 산업적으로 널리 사용되는 퓨트레신을 고수율로 제조하는데 유용하다.
퓨트레신, 변이, 미생물, 포도당-
14.发明公开
公开(公告)号：KR1020100033845A
公开(公告)日：2010-03-31
申请号：KR1020080092906
申请日：2008-09-22
Applicant: 한국과학기술원
CPC classification number: C12N15/67 , C07K14/43518 , C07K14/43586
Abstract: PURPOSE: A method for enhancing expression of a target protein through co-expression of tRNA of a specific amino acid is provided to enhance productivity of specific amino acid. CONSTITUTION: A method for enhancing expression of a target protein is co-expression of a gene encoding the target protein and gene sequence encoding tRNA of a specific amino acid using recombinant microorganism. The recombinant microorganism is obtained by transforming with the gene encoding the target protein and gene sequence encoding tRNA. The target protein is repetitive protein having repetitive structure of a specific oligopeptide. The repeat protein is elastin, silk protein, byssus, flagelliform silk, dragline silk, collagen, keratin, sericin or synthetic repetitive protein. The recombinant microorganism is E.coli. The recombinant vector contains T7 promoter.
Abstract translation: 目的：提供通过特异性氨基酸的tRNA的共表达来增强靶蛋白表达的方法，以提高特异性氨基酸的生产率。 构成：用于增强靶蛋白表达的方法是使用重组微生物共同表达编码靶蛋白的基因和编码特定氨基酸的tRNA的基因序列。 通过用编码目标蛋白的基因和编码tRNA的基因序列转化获得重组微生物。 靶蛋白是具有特异性寡肽重复结构的重复蛋白质。 重复蛋白质是弹力蛋白，丝蛋白，黄豆，鞭毛丝，丝线丝，胶原蛋白，角蛋白，丝胶蛋白或合成重复蛋白。 重组微生物是大肠杆菌。 重组载体含有T7启动子。
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公开(公告)号：KR1020090039027A
公开(公告)日：2009-04-22
申请号：KR1020070104418
申请日：2007-10-17
Applicant: 한국과학기술원
Abstract: A new promoter form expressing and secreting a target protein is provided to produce pure target protein by extracellularly expressing the target protein. Gene constructs comprise: a secretion promoting motif gene selected among a group of a coding gene of arginine transporter subunit(ArtJ), a coding gene of hyperosmotically inducible periplasmic protein(OsmY) and a coding gene of peptidyl-prolyl cis-trans isomerase(FkpA) and a gene coding a target protein. The gene constructs are characterized in intracelluarly expressing in a fusion form of the secretion promoting motif selected and the target protein and extracelluarly secreting. Method of extracellular secretion and production of the target protein comprises: a step of culturing recombinant microorganism containing the gene constructs to extracellularly secret the target protein; and a step of collecting the target protein in culture media.
Abstract translation: 提供表达和分泌靶蛋白的新的启动子形式，通过细胞外表达靶蛋白来产生纯的靶蛋白。 基因构建体包含：选自精氨酸转运子亚基（ArtJ）的编码基因，高肌诱导周质蛋白（OsmY）的编码基因和肽基 - 脯氨酰顺反异构酶（FkpA）的编码基因的分泌促进基序基因 ）和编码靶蛋白的基因。 基因构建体的特征在于以选择的分泌促进基序的融合形式和靶蛋白质和细胞外分泌物进行脑内表达。 细胞外分泌方法和靶蛋白的产生包括：培养含有基因构建体的重组微生物以细胞外分泌靶蛋白的步骤; 以及在培养基中收集靶蛋白的步骤。
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