公开(公告)号：KR1020130140528A

公开(公告)日：2013-12-24

申请号：KR1020120127613

申请日：2012-11-12

Applicant: 한국전자통신연구원

Inventor： 김창범 ,  송기봉 ,  김관수

IPC: G01N33/68 ,  G01N33/533 ,  G01N21/64

Abstract: Provided is a method for diagnosing Alzheimer's disease using a decomposed biomaterial. The method comprises: preparing magnetic particles on which primary capture antibodies specifically bound to beta-amyloid are adsorbed; decomposing a biomaterial including beta-amyloid to bind the beta-amyloid present in the biomaterial to the primary capture antibodies; binding secondary capture antibodies labeled with a fluorescent material to the magnetic particles bound to the beta-amyloid to form a complex; disposing the complex in a channel region of a photo field effect transistor in which photocurrent is changed according to the amount of incident light; and measuring the photocurrent changed by light excited from the complex to quantify the concentration of the beta-amyloid present in the biomaterial. [Reference numerals] (S110) Prepare magnetic particles;(S120) Decompose biomaterial containing beta-amyloid protein;(S130) Forming a complex of the magnetic particles and multiple proteins;(S140) Disposing the complex in a sensing region of a photo field effect transistor;(S150) Measuring the photocurrent of the photo field effect transistor

Abstract translation: 提供了使用分解生物材料诊断阿尔茨海默氏病的方法。 该方法包括：制备其上吸附了特异性结合β-淀粉样蛋白的初级捕获抗体的磁性颗粒; 分解生物材料，包括β-淀粉样蛋白，将生物材料中存在的β-淀粉样蛋白结合到初级捕获抗体; 用荧光材料标记的结合二次捕获抗体结合到β-淀粉样蛋白上形成复合物的磁性颗粒; 将复合物设置在光电流根据入射光量变化的光场效应晶体管的沟道区域中; 并测量由复合物激发的光改变的光电流，以量化存在于生物材料中的β-淀粉样蛋白的浓度。 （S110）准备磁性粒子;（S120）分解含有β-淀粉样蛋白的生物材料;（S130）形成磁性粒子和多种蛋白质的复合体;（S140）在复合体的感光区域中配置复合体 效应晶体管;（S150）测量光场效应晶体管的光电流