Abstract:
PURPOSE: A nanoparticle-nucleic acid complex and a method for linearizing target nucleic acids using the same are provided to enable effectively sequencing and mapping of the target nucleic acids. CONSTITUTION: A nanoparticle-nucleic acid complex contains nanoparticles(100) in which two or more protection sequence parts(110) and a probe sequence part(120) are fixed. The protection sequence parts are fixed at the nanoparticfles at one end and have a single strand nucleic acid containing a random base sequence. The probe sequence part is fixed at the nanoparticles at one end and has a single strand nucleic acid containing a base sequenc ewhic complementarily binds to a first region(131) of connection sequence part(130). The protection sequence part further contains a spacer. The spacer binds on the surface of the nanoparticles at one end and binds the single stand nucleic acid of the production sequene at the other end.
Abstract:
본 발명은 신규한 리피드-금나노입자 복합체, 그 제조방법 및 상기 복합체를 이용한 유전자 등의 생리활성물질의 전달 방법에 관한 것이다. 구체적으로, 본 발명의 일 실시예에 따른 금나노입자 복합체는 금나노입자 및 상기 금나노입자 표면에 정전기적 상호작용에 의하여 결합된 생리활성물질을 포함한다. 또한, 본 발명의 다른 일 실시예에 따른 리피드-금나노입자 복합체는 금나노입자; 상기 금나노입자 표면에 결합된 생리활성물질; 및 상기 금나노입자 및 생리활성물질을 둘러싸서 캡슐화하는 리피드를 포함한다. 본 발명에 따른 유전자 등의 생리활성물질 전달 방법은 금나노입자 복합체에 초음파를 가하거나, 또는 금나노입자와 리피드를 운반체로 이용함으로써, 전달 효율이 현저하게 향상되며, 세포 독성을 최소화할 수 있어 최적화된 전달 시스템으로서 광범위하게 이용될 수 있다. 금나노입자, 리피드, 복합체, 초음파, 생리활성물질, 전달
Abstract:
PURPOSE: A method for delivery of a physiological active substance using a novel lipid-gold nanoparticle complex is provided to improve delivery efficiency and to minimize cytotoxicity. CONSTITUTION: A gold nanoparticle complex contains gold nanoparticle physiological active substance conjugated to the surface of the gold nanoparticle. The physiological active substance is DNA, RNA, plasmid DNA, peptide, protein, polynucleotide or oligonucleotide. The complex further contains a polyethylene glycol(PEG) conjugated at the surface of the gold nanoparticle. A method for preparing the complex comprises: a step of mixing the gold nanoparticle and physiological active substance; a step of binding the physiological active substance on the surface of the gold nanoparticle by electrostatic interaction; and a step of encapsulating the gold nanoparticle and physiological substance with lipid.