公开(公告)号：WO1992021980A1

公开(公告)日：1992-12-10

申请号：PCT/US1992002982

申请日：1992-04-10

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  JOU, Yi-Her ,  STROUPE, Stephen, D.

IPC: G01N33/566

CPC classification number: G01N33/558 ,  G01N33/538 ,  G01N33/54366 ,  G01N33/57473

Abstract: This invention presents novel assay devices employing a capture reagent, involving a specific binding member attached to a charged substance, and a porous material containing a capture or reaction zone that is oppositely charged with respect to the capture reagent. In one embodiment, a test sample suspected of containing the analyte of interest is contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged capture or reaction zone to attract, attach, and immobilize the capture reagent/analyte complex. With an appropriate indicator reagent, both sandwich and competitive assays can be performed.

Abstract translation: 本发明提出了采用捕获试剂的新型测定装置，其涉及与带电物质连接的特异性结合成分，以及含有相对于捕获试剂相反带电的捕获或反应区的多孔材料。 在一个实施方案中，怀疑含有感兴趣分析物的测试样品与捕获试剂接触以形成带电的捕获试剂/分析物复合物。 然后将复合物与相反电荷的捕获或反应区接触以吸引，附着和固定捕获试剂/分析物复合物。 使用适当的指示剂，可以进行三明治和竞争性测定。

公开(公告)号：WO1986007387A1

公开(公告)日：1986-12-18

申请号：PCT/US1986001280

申请日：1986-06-13

Applicant: AMGEN ,  ABBOTT LABORATORIES

Inventor： AMGEN ,  ABBOTT LABORATORIES ,  SNITMAN, David, L. ,  STROUPE, Stephen, D.

IPC: C12Q01/68

CPC classification number: G01N33/54353 ,  C12Q1/6804 ,  C12Q1/6813 ,  C12Q1/705 ,  G01N33/532

Abstract: A method and a kit for the isolation and quantitative detection of a selected target nucleic acid sequence from solution employing two probes. A first probe is complementary to one portion of the target and is covalently attached to a first complexing agent (e.g., either an antigen or an antibody). The second probe is complementary to a different portion of the target and is associated with a reporter group. Following hybridization of the target and two probes in solution, a solid support coated with a second complexing agent (i.e., a corresponding antibody or antigen) capable of binding to the first complexing agent on the first probe is employed to immobilize the target-probe hybrid complex. A plurality of types of first probes may be used. Each type is attached to the same sort of complexing agent but each includes a nucleic acid sequence which is complementary to a different portion of the target.

Abstract translation: 用于使用两个探针从溶液中分离和定量检测所选靶核酸序列的方法和试剂盒。 第一个探针与靶的一部分互补，并且共价连接到第一络合剂（例如抗原或抗体）上。 第二个探针与目标的不同部分相互补充，并与报告者组相关联。 在溶液中杂交靶和两个探针之后，使用能够与第一探针上的第一络合剂结合的第二络合剂（即，相应的抗体或抗原）涂覆的固体支持物来固定靶 - 探针杂交体 复杂。 可以使用多种类型的第一探针。 每种类型都与相同种类的络合剂连接，但每种都包含与目标不同部分互补的核酸序列。

公开(公告)号：EP1200113A2

公开(公告)日：2002-05-02

申请号：EP00916660.4

申请日：2000-03-24

Applicant: ABBOTT LABORATORIES

Inventor： BILLING-MEDEL, Patricia, A. ,  COHEN, Maurice ,  COLPITTS, Tracey, L. ,  GORDON, Julian ,  GRANADOS, Edward, N. ,  RUSSELL, John, C. ,  STROUPE, Stephen, D.

IPC: A61K38/17 ,  C07K14/47 ,  C07K16/30 ,  C12N5/10 ,  C12N15/63 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/574 ,  G01N33/577

CPC classification number: G01N33/57434 ,  A61K38/00 ,  C07K14/4748 ,  C07K16/3069 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/136

Abstract: A set of contiguous and partially overlapping cDNA sequences and polypeptides encoded thereby, designated as PCIGF and transcribed from prostate tissue, is described. These sequences are useful for the detecting, diagnosing, staging, monitoring, prognosticating, in vivo imaging, preventing or treating, or determining the predisposition of an individual to diseases and conditions of the prostate, such as prostate cancer. Also provided are antibodies which specifically bind to PCIGF-encoded polypeptide or protein, and agonists or inhibitors which prevent action of the tissue-specific PCIGF polypeptide, which molecules are useful for the therapeutic treatment of prostate diseases, tumors or metastases.

公开(公告)号：EP1027457A1

公开(公告)日：2000-08-16

申请号：EP98957404.1

申请日：1998-10-28

Applicant: ABBOTT LABORATORIES

Inventor： BILLING-MEDEL, Patricia, A. ,  COHEN, Maurice ,  COLPITTS, Tracey, L. ,  FRIEDMAN, Paula, N. ,  GORDON, Julian ,  GRANADOS, Edward, N. ,  HODGES, Steven, C. ,  KLASS, Michael, R. ,  KRATOCHVIL, Jon, D. ,  RUSSELL, John, C. ,  STROUPE, Stephen, D. ,  YU, Hong

IPC: C12Q1/68 ,  G01N33/574 ,  C07K14/47

CPC classification number: C12N9/6424 ,  C07K14/47 ,  C12Q1/6886 ,  C12Q2600/136 ,  G01N33/57415

Abstract: A set of contiguous and partially overlapping cDNA sequences and polypeptides encoded thereby, designated as BS247 and transcribed from breast tissue, are described. These sequences are useful for the detecting, diagnosing, staging, monitoring, prognosticating, in vivo imaging, preventing or treating, or determining the predisposition of an individual to diseases and conditions of the breast, such as breast cancer. Also provided are antibodies which specifically bind to BS247-encoded polypeptide or protein, and agonists or inhibitors which prevent action of the tissue-specific BS247 polypeptides, which molecules are useful for the therapeutic treatment of breast diseases, tumors or metastases.

公开(公告)号：EP0641442B1

公开(公告)日：1997-12-17

申请号：EP92911929.5

申请日：1992-04-10

Applicant: ABBOTT LABORATORIES

Inventor： JOU, Yi-Her ,  STROUPE, Stephen, D.

IPC: G01N33/566 ,  G01N33/543 ,  G01N33/544 ,  G01N33/545 ,  G01N33/558

CPC classification number: G01N33/558 ,  G01N33/538 ,  G01N33/54366 ,  G01N33/57473

Abstract: This invention presents novel assay devices employing a capture reagent, involving a specific binding member attached to a charged substance, and a porous material containing a capture or reaction zone that is oppositely charged with respect to the capture reagent. In one embodiment, a test sample suspected of containing the analyte of interest is contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged capture or reaction zone to attract, attach, and immobilize the capture reagent/analyte complex. With an appropriate indicator reagent, both sandwich and competitive assays can be performed.

公开(公告)号：EP0586605A1

公开(公告)日：1994-03-16

申请号：EP92914861.0

申请日：1992-04-10

Applicant: ABBOTT LABORATORIES

Inventor： ADAMCZYK, Janina ,  BERRY, Daniel, S. ,  FICO, Rosario ,  JOU, Yi-Her ,  STROUPE, Stephen, D.

IPC: G01N33

CPC classification number: G01N33/538

Abstract: Nouveaux procédés d'analyse faisant appel à un réactif de capture, mettant en jeu un premier membre de fixation normalement associé à une substance anionique polymérique, un matériau en phase solide contenant un lieu de réaction constitué d'une substance cationique polymérique d'une teneur en azote d'au moins environ 2 %. Dans un mode de réalisation, un échantillon à analyser dans lequel on soupçonne la présence de l'analyte recherché peut être mis en contact avec le réactif de capture pour former un complexe chargé contenant le réactif de capture et l'analyte. Le complexe est ensuite mis en contact avec la phase solide de charge opposée pour attirer, fixer et immobiliser le complexe réactif de capture/analyte. L'utilisation d'une substance polycationique, d'une teneur en hydrogène d'au moins environ 2 % (à l'exclusion des contre-ions), dans la préparation d'une phase solide correctement chargée fournit une phase solide qui peut être soumise à un plus grande nombre de manipulations, tels que les lavages multiples, sans perdre la capacité d'attirer et de retenir le réactif de capture.

公开(公告)号：EP0586574A1

公开(公告)日：1994-03-16

申请号：EP92913231.0

申请日：1992-04-10

Applicant: ABBOTT LABORATORIES

Inventor： KLINE, Steven ,  JOU, Yi-Her ,  STROUPE, Stephen, D.

IPC: G01N33

CPC classification number: G01N33/543 ,  G01N33/532 ,  G01N33/545 ,  G01N33/56988 ,  G01N33/57473 ,  G01N33/58 ,  G01N33/743 ,  G01N33/76 ,  G01N33/9453

Abstract: Nouveaux procédés d'analyse de la digoxine faisant appel à un réactif de capture, mettant en jeu un premier membre de fixation associé à une substance anionique polymérique, et un matériau en phase solide contenant un lieu de réaction constitué d'une substance cationique polymérique d'une teneur en azote d'au moins environ 2 %. Un échantillon à analyser dans lequel on soupçonne la présence de l'analyte recherché peut être mis en contact avec le réactif de capture afin de former un complexe chargé contenant le réactif de capture et l'analyte. Le complexe est ensuite mis en contact avec la phase solide de charge opposée afin d'attirer, de fixer et d'immobiliser le complexe réactif de capture/analyte.

Abstract translation: 使用捕获试剂进行地高辛分析的新方法涉及与聚合物阴离子物质缔合的第一连接部件和含有由聚合物阳离子物质组成的反应部位的固相材料 氮含量至少约2％。 其中怀疑存在所需分析物的待分析样品可以与捕获剂接触以形成含有捕获剂和分析物的带电复合物。 然后使该复合物与带相反电荷的固相接触以吸引，固定和固定捕捉剂/分析物复合物。

公开(公告)号：EP1141389A2

公开(公告)日：2001-10-10

申请号：EP00908317.1

申请日：2000-01-19

Applicant: ABBOTT LABORATORIES

Inventor： BILLING-MEDEL, Patricia, A. ,  COHEN, Maurice ,  COLPITTS, Tracey, L. ,  FRIEDMAN, Paula, N. ,  GORDON, Julian ,  GRANADOS, Edward, N. ,  HODGES, Steven, C. ,  KLASS, Michael, R. ,  KRATOCHVIL, Jon, D. ,  ROBERTS-RAPP, Lisa ,  RUSSELL, John, C. ,  SCHEFFEL, Christi, P. ,  STROUPE, Stephen, D.

IPC: C12Q1/68 ,  C07K14/47 ,  C12N5/10 ,  C07K16/18

CPC classification number: C07K14/47

Abstract: A set of contiguous and partially overlapping cDNA sequences and polypeptides encoded thereby, designated as BU101 and transcribed from breast tissue, is described. These sequences are useful for the detecting, diagnosing, staging, monitoring, prognosticating, in vivo imaging, preventing or treating, or determining the predisposition of an individual to diseases and conditions of the breast, such as breast cancer. Also provided are antibodies which specifically bind to BU101-encoded polypeptide or protein, and agonists or inhibitors which prevent action of the tissue-specific BU101 polypeptide, which molecules are useful for the therapeutic treatment of breast diseases, tumors or metastases.

公开(公告)号：EP0990045A1

公开(公告)日：2000-04-05

申请号：EP98931212.9

申请日：1998-06-11

Applicant: ABBOTT LABORATORIES

Inventor： BILLING-MEDEL, Patricia, A. ,  COHEN, Maurice ,  COLPITTS, Tracey, L. ,  FRIEDMAN, Paula, N. ,  GORDON, Julian ,  GRANADOS, Edward, N. ,  HODGES, Steven, C. ,  KLASS, Michael, R. ,  KRATOCHVIL, Jon, D. ,  ROBERTS-RAPP, Lisa ,  RUSSELL, John, C. ,  STROUPE, Stephen, D.

IPC: C12Q1/68 ,  C07K14/47 ,  C12N5/10 ,  C07K16/00 ,  G01N33/574

CPC classification number: C07K16/3023 ,  C07K2317/34 ,  C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/158

Abstract: A set of contiguous and partially overlapping cDNA sequences and polypeptides encoded thereby, designated as LS170 and transcribed from lung tissue, is described. These sequences are useful for the detecting, diagnosing, staging, monitoring, prognosticating, in vivo imaging, preventing or treating, or determining the predisposition of an individual to diseases and conditions of the lung, such as lung cancer. Also provided are antibodies which specifically bind to a LS170-encoded polypeptide or protein, and agonists or inhibitors which prevent action of tissue-specific LS170 polypeptides, which molecules are useful for the therapeutic treatment of lung diseases, tumors, or metastases.

Abstract translation: 描述了一组连续和部分重叠的cDNA序列和由此编码的多肽，命名为LS170并从肺组织转录。 这些序列可用于检测，诊断，分期，监测，预后，体内成像，预防或治疗或确定个体对肺的疾病和状况（例如肺癌）的倾向。 还提供了特异性结合LS170编码的多肽或蛋白质的抗体，以及预防组织特异性LS170多肽的作用的激动剂或抑制剂，该分子可用于治疗肺部疾病，肿瘤或转移瘤。

公开(公告)号：EP0972056A1

公开(公告)日：2000-01-19

申请号：EP98907345.7

申请日：1998-01-30

Applicant: ABBOTT LABORATORIES

Inventor： BILLING-MEDEL, Patricia, A. ,  COHEN, Maurice ,  COLPITTS, Tracey, L. ,  FRIEDMAN, Paula, N. ,  GORDON, Julian ,  GRANADOS, Edward, N. ,  HODGES, Steven, C. ,  KLASS, Michael, R. ,  KRATOCHVIL, Jon, D. ,  ROBERTS-RAPP, Lisa ,  RUSSELL, John, C. ,  STROUPE, Stephen, D.

IPC: C12N15/63 ,  C12N5/10 ,  C12Q1/68 ,  C07K14/47

CPC classification number: C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/158

Abstract: A set of contiguous and partially overlapping cDNA sequences and polypeptides encoded thereby, designated as LU105 and transcribed from lung tissue, is described. These sequences are useful for the detecting, diagnosing, staging, monitoring, prognosticating, preventing or treating, or determining the predisposition of an individual to diseases and conditions of the lung, such as lung cancer. Also provided are antibodies which specifically bind to LU105-encoded polypeptide or protein, and agonists or inhibitors which prevent action of the tissue-specific LU105 polypeptide, which molecules are useful for the therapeutic treatment of lung diseases, tumors or metastases.