公开(公告)号：JP2007252388A

公开(公告)日：2007-10-04

申请号：JP2007141287

申请日：2007-05-29

Applicant: Basf Ag ,  ビーエーエスエフ アクチェンゲゼルシャフトBASF Aktiengesellschaft

Inventor： POMPEJUS MARKUS ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  HABERHAUER GREGOR

IPC: C12N15/09 ,  G01N33/53 ,  C07K20060101 ,  C07K14/34 ,  C12N20060101 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/12 ,  C12N9/18 ,  C12N9/88 ,  C12N15/31 ,  C12N15/55 ,  C12P20060101 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P21/02 ,  C12Q20060101 ,  C12Q1/68 ,  C12R1/15 ,  G01N33/566 ,  G01N33/569

CPC classification number: C12N9/88 ,  C07K14/34 ,  C12N9/16 ,  C12N9/18

Abstract: PROBLEM TO BE SOLVED: To provide SMP nucleic acid and protein molecules, which relate to metabolism of carbon compounds such as sugars and generation of energy molecules, in a process (such as oxidative phosphorylation) of Corynebacterium glutamicum. SOLUTION: Isolated nucleic acid molecules of a specific sequence, or a complement thereof, which nucleic acid molecules are selected from a sequence group relating to metabolism of carbon compounds such as sugars and generation of energy molecules, in a process of Corynebacterium glutamicum, are provided, and cells containing the nucleic acid molecules and of genus Corynebacterium or genus Brevibacterium are provided. A method for producing fine chemicals such as an amino acid by using the cells, and a method for detecting existence and activity of Corynebacterium diphtheriae is provided. COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：在谷氨酸棒杆菌的过程（如氧化磷酸化）中提供涉及碳化合物如糖的代谢和产生能量分子的SMP核酸和蛋白质分子。 解决方案：在谷氨酸棒杆菌的过程中，核酸分子选自与碳化合物的代谢例如糖和能量分子的产生有关的序列组的特异性序列或其互补物的分离的核酸分子 ，并且提供含有核酸分子和棒状杆菌属或短杆菌属的细胞。 提供了通过使用细胞产生氨基酸等精细化学品的方法，以及用于检测白喉杆菌的存在和活性的方法。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2007252384A

公开(公告)日：2007-10-04

申请号：JP2007123626

申请日：2007-05-08

Applicant: Basf Ag ,  ビーエーエスエフ アクチェンゲゼルシャフトBASF Aktiengesellschaft

Inventor： POMPEJUS MARKUS ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  HABERHAUER GREGOR

IPC: C12N15/09 ,  G01N33/53 ,  C07H21/04 ,  C07K20060101 ,  C07K14/34 ,  C12N20060101 ,  C12N1/15 ,  C12N1/19 ,  C12N1/20 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/10 ,  C12N9/18 ,  C12N9/90 ,  C12N15/31 ,  C12N15/61 ,  C12N15/74 ,  C12P20060101 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/14 ,  C12P13/20 ,  C12P13/22 ,  C12P13/24 ,  C12P21/02 ,  C12P21/06 ,  C12Q20060101 ,  C12Q1/68 ,  C12R1/15 ,  G01N33/566 ,  G01N33/569

CPC classification number: C12N9/18 ,  C07K14/34 ,  C12N9/00 ,  C12N9/1096 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12P13/24 ,  C12Y206/01005

Abstract: PROBLEM TO BE SOLVED: To provide a new bacterial nucleic acid molecule having various applications. SOLUTION: Isolated nucleic acid molecules, designated MP (melabolic pathway) nucleic acid molecules, which encode new MP proteins derived from Corynebacterium glutamicum, are described. Antisense nucleic acid molecules, recombinant expression vectors containing MP nucleic acid molecules, and host cells into which the expression vectors are introduced, are also provided. Further, isolated MP proteins, mutated MP proteins, fusion proteins, antigenic peptides and methods for improving production of a desired compound from C. glutamicum based on genetic engineering of MP genes in the organism, are provided. COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 待解决的问题：提供具有各种应用的新的细菌核酸分子。 解决方案：描述编码来自谷氨酸棒杆菌的新MP蛋白质的分离的核酸分子，称为MP（代谢途径）核酸分子。 还提供了反义核酸分子，含有MP核酸分子的重组表达载体和其中引入表达载体的宿主细胞。 此外，提供了基于生物体中MP基因的遗传工程的分离的MP蛋白，突变的MP蛋白，融合蛋白，抗原肽和用于从谷氨酸棒球菌（C.Gyamicum）生产所需化合物的方法。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2007244391A

公开(公告)日：2007-09-27

申请号：JP2007121127

申请日：2007-05-01

Applicant: Basf Ag ,  ビーエーエスエフ アクチェンゲゼルシャフトBASF Aktiengesellschaft

Inventor： POMPEJUS MARKUS ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  HABERHAUER GREGOR ,  LEE HEUNG-SHICK ,  KIM HYUNG-JOON

IPC: C12N15/09 ,  G01N33/53 ,  C07K20060101 ,  C07K14/34 ,  C12N20060101 ,  C12N1/12 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/18 ,  C12N15/31 ,  C12P20060101 ,  C12P1/04 ,  C12P13/04 ,  C12P21/02 ,  C12Q20060101 ,  C12Q1/04 ,  C12Q1/68 ,  C12R1/13 ,  C12R1/15 ,  C12R1/16 ,  G01N33/569 ,  G01N37/00

CPC classification number: C12N9/00 ,  C07K14/34 ,  C12N9/18

Abstract: PROBLEM TO BE SOLVED: To provide a stress, resistance and tolerance protein encoding Corynebacterium glutamicum gene used for the discrimination of microorganism for the production of fine chemicals, control of the production of fine chemicals by bacteria, classification or discrimination of bacteria, use as a reference point for the preparation of a genom map and as a label for genetic transformation. SOLUTION: The invention provides an isolated nucleic acid molecule or a designated SRT nucleic acid molecule encoding a new SRT protein derived from Corynebacterium glutamicum. The invention also provides its antisense nucleic acid molecule, a recombinant expression vector containing SRT nucleic acid molecule, and host cell into which the expression vector is to be introduced. The invention still further provides isolated SRT protein, mutated SRT protein, fusion protein, antigenic peptide and method for the improvement of the production of a desired compound from C. glutamicum based on genetic engineering of SRT genes of this organism. COPYRIGHT: (C)2007,JPO&INPIT

Abstract translation: 要解决的问题：提供用于鉴别用于生产精细化学品的微生物的谷氨酸棒杆菌基因的应力，抗性和耐受蛋白质编码，细菌生产精细化学物质的控制，细菌的分类或鉴别， 用作制备基因图的参考点和作为遗传转化的标签。 解决方案：本发明提供了分离的核酸分子或编码来自谷氨酸棒杆菌的新SRT蛋白的指定的SRT核酸分子。 本发明还提供了其反义核酸分子，含有SRT核酸分子的重组表达载体和其中将引入表达载体的宿主细胞。 本发明还进一步提供了基于该生物体的SRT基因的遗传工程的分离的SRT蛋白，突变的SRT蛋白，融合蛋白，抗原肽和从谷氨酸棒球菌产生所需化合物的方法。 版权所有（C）2007，JPO＆INPIT

公开(公告)号：JP2007236393A

公开(公告)日：2007-09-20

申请号：JP2007115387

申请日：2007-04-25

Applicant: Basf Ag ,  ビーエーエスエフ アクチェンゲゼルシャフトBASF Aktiengesellschaft

Inventor： POMPEJUS MARKUS ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  HABERHAUER GREGOR

IPC: C12N15/09 ,  G01N33/53 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/10 ,  C12N9/12 ,  C12N15/31 ,  C12N15/54 ,  C12P1/04 ,  C12P13/04 ,  C12Q1/04 ,  C12Q1/48 ,  C12Q1/68 ,  C12R1/13 ,  C12R1/16 ,  G01N33/566 ,  G01N33/569

CPC classification number: C12N9/1223 ,  C12N9/12 ,  C12Y207/03009

Abstract: PROBLEM TO BE SOLVED: To provide a novel nucleic acid molecule derived from Corynebacterium glutamicum. SOLUTION: Isolated nucleic acid molecules encoding novel PTS proteins (phosphoenolpyruvate: sugar phosphotransferase) derived from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing PTS nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated PTS proteins, mutated PTS proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of the PTS genes. COPYRIGHT: (C)2007,JPO&INPIT

Abstract translation: 待解决的问题：提供源自谷氨酸棒杆菌的新型核酸分子。 解决方案：描述了编码源自谷氨酸棒杆菌的新型PTS蛋白（磷酸烯醇丙酮酸：糖磷酸转移酶）的分离的核酸分子。 本发明还提供反义核酸分子，含有PTS核酸分子的重组表达载体，以及引入了表达载体的宿主细胞。 本发明还进一步提供了基于PTS基因的遗传工程的分离的PTS蛋白，突变的PTS蛋白，融合蛋白，抗原肽和用于从谷氨酸棒球菌（C.GGG）生产所需化合物的方法。 版权所有（C）2007，JPO＆INPIT

公开(公告)号：WO03097678A2

公开(公告)日：2003-11-27

申请号：PCT/EP0304969

申请日：2003-05-13

Applicant: BASF AG ,  KLOPPROGGE CORINNA ,  ZELDER OSKAR ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  LEE HEUNG-SHICK

Inventor： KLOPPROGGE CORINNA ,  ZELDER OSKAR ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  LEE HEUNG-SHICK

IPC: C12N15/09 ,  C07K14/34 ,  C12N15/31 ,  C12P13/08

CPC classification number: C07K14/34

Abstract: Isolated polypeptide sequence having the sequence of SEQ ID NO:1 or muteins thereof having the ability to bind cAMP and repress the expression of the aceB gene of C. glutamicum and which can be obtained from SEQ ID NO:1 by inserting, deleting or substituting up to 20% of the amino acids.

Abstract translation: 具有SEQ ID NO：1的序列的分离的多肽序列或其突变蛋白具有结合cAMP的能力并抑制谷氨酸棒杆菌的aceB基因的表达，并且可以通过插入，缺失或替代从SEQ ID NO：1获得 高达20％的氨基酸。

公开(公告)号：WO2004024931A3

公开(公告)日：2004-04-22

申请号：PCT/EP0309451

申请日：2003-08-26

Applicant: BASF AG ,  KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

Inventor： KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: A23K1/00 ,  A23K1/16 ,  C12N15/09 ,  C12P13/04 ,  C12P13/12 ,  C12R1/15

CPC classification number: C12P13/04 ,  C12P13/12

Abstract: The invention relates to methods for the production by fermentation of sulphur-containing fine chemicals, in particular L-methionine, using bacteria in which a nucleic acid sequence coding for a methionine synthase gene (metF) is expressed.

Abstract translation: 本发明涉及一种用于发酵生产的含硫精细化学品的方法，特别是L-甲硫氨酸，利用细菌，其中用于甲硫氨酸合酶一个（metF）基因编码核苷酸序列被表达。

公开(公告)号：WO03040681A2

公开(公告)日：2003-05-15

申请号：PCT/EP0212141

申请日：2002-10-31

Applicant: BASF AG ,  ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR

Inventor： ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR

IPC: C12N1/21 ,  C12N15/52 ,  C12N15/77 ,  C12P13/08 ,  G01N

CPC classification number: C12N15/77 ,  C12N15/52 ,  C12P13/08

Abstract: The invention relates to novel nucleic acid molecules, the use thereof in the construction of bio-engineering improved microorganisms and methods for the production of fine chemicals, especially amino acids with the aid of said bio-engineered improved microorganisms.

Abstract translation: 本发明涉及新的核酸分子，它们的遗传改良的微生物以及用于产生精细化学品，在使用这些遗传改良的微生物的特定氨基酸的方法的结构中使用。

公开(公告)号：WO0224917A3

公开(公告)日：2002-06-27

申请号：PCT/EP0110805

申请日：2001-09-19

Applicant: BASF AG ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  ZELDER OSKAR

Inventor： POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  ZELDER OSKAR

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12N15/77 ,  C12P1/04 ,  C12P13/04 ,  C12P13/08 ,  C12P13/12 ,  C12P13/14 ,  C12N15/68

CPC classification number: C12N9/1007 ,  C12N15/77

Abstract: The invention relates to a method for producing corynebacteria that contain one or more modified genomic sequences, characterized by using a vector that does not replicate in corynebacteria and whose nucleic acid is not recognized as foreign by the corynebacteria.

Abstract translation: 本发明涉及含有一种或使用非复制在棒状杆菌载体用于制备棒状杆菌的方法，所述核酸不是由棒状杆菌识别为外来更多改变的基因组序列。

公开(公告)号：WO0102583A3

公开(公告)日：2001-07-26

申请号：PCT/IB0000973

申请日：2000-06-27

Applicant: BASF AG

Inventor： POMPEJUS MARKUS ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  HABERHAUER GREGOR

IPC: G01N33/53 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/10 ,  C12N9/12 ,  C12N15/09 ,  C12N15/31 ,  C12N15/54 ,  C12P1/04 ,  C12P13/04 ,  C12Q1/04 ,  C12Q1/68 ,  C12R1/13 ,  C12R1/16 ,  G01N33/566 ,  G01N33/569 ,  C12N15/77

CPC classification number: C12N9/1223 ,  C12N9/12 ,  C12Y207/03009

Abstract: Isolated nucleic acid molecules, designated PTS nucleic acid molecules, which encode novel PTS proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing PTS nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated PTS proteins, mutated PTS proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of PTS genes in this organism.

Abstract translation: 描述了编码来自谷氨酸棒杆菌的新型PTS蛋白质的分离的核酸分子，命名为PTS核酸分子。 本发明还提供反义核酸分子，含有PTS核酸分子的重组表达载体，以及引入了表达载体的宿主细胞。 本发明还进一步提供了基于该生物体中PTS基因的遗传工程的分离的PTS蛋白，突变的PTS蛋白，融合蛋白，抗原肽和用于从谷氨酸棒状病毒从谷氨酸棒球菌生产所需化合物的方法。

公开(公告)号：WO9964572A3

公开(公告)日：2000-06-08

申请号：PCT/EP9903889

申请日：1999-06-04

Applicant: BASF AG ,  KOCK MICHAEL ,  HOEGER THOMAS ,  KROEGER BURKHARD ,  OTTERBACH BERND ,  LUBISCH WILFRIED ,  LEMAIRE HANS GEORG

Inventor： KOCK MICHAEL ,  HOEGER THOMAS ,  KROEGER BURKHARD ,  OTTERBACH BERND ,  LUBISCH WILFRIED ,  LEMAIRE HANS-GEORG

IPC: A01K67/027 ,  A61K38/00 ,  A61K38/45 ,  A61K48/00 ,  A61K49/00 ,  A61P43/00 ,  C07K14/455 ,  C12N1/15 ,  C12N1/19 ,  C12N5/10 ,  C12N9/10 ,  C12N15/09 ,  C12N15/54 ,  C12Q1/68 ,  G01N33/53 ,  A61K31/70 ,  C12N15/10

CPC classification number: C12N9/1077 ,  A01K2217/05 ,  A61K38/00 ,  A61K49/00 ,  C07K16/40 ,  C07K2317/76 ,  C07K2319/00 ,  C12Q1/48 ,  C12Y204/0203 ,  G01N2333/91142

Abstract: The invention relates to poly(ADP-ribose)polymerase (PARP) homologues which are characterised by an amino acid sequence with a) a functional NAD -binding site and b) no zinc-finger-sequence motif of general formula CX2CXmHX2C, wherein m is an integral number 28 or 30 and the radicals X represent any amino acid, independently of each other; and to the functional equivalents of said poly(ADP-ribose)polymerase (PARP) homologues. The invention also relates to nucleic acids coding the poly(ADP-ribose)polymerase (PARP) homologues, to antibodies with specificity for the novel protein, to pharmaceutical and gene therapy agents containing the inventive products, to methods for analytically determining the inventive proteins and nucleic acids, to methods for identifying the effectors or bonding partners of the inventive proteins, to novel PARP effectors and to methods for determining the effectiveness of effectors of this type.

Abstract translation: 本发明涉及聚（ADP-核糖）聚合酶（PARP）的同系物，其特征在于包括的氨基酸序列的）功能性NAD <+> - 结合结构域和b）通式的无锌指基序：CX2CXmHX2C具有，其中 m为28或30的整数，并且基团X独立地是任意氨基酸; 和其功能等价物; 核酸编码; 具有特异性的新型蛋白质的抗体; 包含根据本发明的产品的药物和基因治疗的组合物; 一种用于分析测定本发明的蛋白质和核酸的方法; 一种用于鉴定效应或结合本发明的蛋白质的方法伙伴; 新颖PARP效应; 和用于确定这种效应物的有效性的方法。