公开(公告)号：AU3970695A

公开(公告)日：1996-05-23

申请号：AU3970695

申请日：1995-10-27

Applicant: BAXTER INT

Inventor： SMITH SIDNEY T ,  BACEHOWSKI DAVID V ,  KOLANKO WILLIAM ,  ROSENBAUM LARRY ,  SMITH STEPHEN L

IPC: B32B27/28 ,  B32B27/30 ,  B32B27/32 ,  C12M1/00 ,  C12M1/12 ,  C12M3/06

Abstract: A multi-layer, flexible, gas-permeable film (10) suitable for forming a cell culture container (20), the film (10) comprising a first layer (12) composed of a polystyrene having a thickness within the range of 0.0001 inches to about 0.0010 inches and, a second layer (14) adhered to the first layer (12) composed of a polyolefin having a thickness within the range of 0.004 inches to about 0.015 inches.

公开(公告)号：CA2168091A1

公开(公告)日：1995-03-02

申请号：CA2168091

申请日：1994-08-23

Applicant: BAXTER INT

Inventor： SMITH STEPHEN L ,  QIAO XIAOYING ,  MACIUKAS SUSAN M ,  BENDER JAMES G ,  VAN EPPS DENNIS E ,  LOUDOVARIS MAUREEN F

IPC: C12Q1/06 ,  A61K35/12 ,  A61K35/14 ,  C12N5/078 ,  C12N5/0787 ,  C12N5/08 ,  C12N5/02

Abstract: The invention provides serum-free, animal protein-free media formulations to be used in conjunction with hematopoietic growth factors for the in vitro growth of human neutrophil and megakaryocyte precursors. The medium is comprised of a base medium, corticosteriod, transferrin, insulin, cholesterol, ethanolamine, and human albumin. The invention also provides methods for preparing serum-free, animal protein-free suspensions of human hematopoietic precursor cells wherein the cellular component comprises at least about 16 % neutrophil precursors and at least about 1 % megakaryocyte precursors. Serum-free, animal protein-free suspensions of human hematopoietic cells are provided wherein the cellular component comprises at least about 30 %, preferably greater than 60 % neutrophil precursors. The neutrophil precursors are comprised of blast cells, promyelocytes, neutrophilic myelocytes, and neutrophilic metamyelocytes. Also provided are serum-free, animal protein-free cell suspensions wherein the cellular component comprises at least about 3 %, preferably greater than 8 % megakaryocyte precursors. Also provided are serum-free, animal-protein free cell suspensions wherein the cellular component comprises colony-forming units and cluster-forming units.