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    FLOW-THROUGH BIOREACTOR WITH GROOVES FOR CELL RETENTION
    2.
    发明公开
    FLOW-THROUGH BIOREACTOR WITH GROOVES FOR CELL RETENTION 失效
    FLOW生物反应器SLAT克制细胞的

    公开(公告)号：EP0698085A4

    公开(公告)日：1996-08-21

    申请号：EP95912718

    申请日：1995-03-03

    Applicant: BAXTER INT ,  UNIV NORTHWESTERN

    Inventor： SANDSTROM CRAIG ,  PAPOUTSAKIS E T ,  MILLER WILLIAM M ,  BENDER JAMES G

    IPC: C12M3/00 ,  C12M3/04 ,  C12N5/06 ,  C12N5/00 ,  C12N5/12 ,  C12N5/16

    CPC classification number: C12M23/12 ,  C12M23/04 ,  C12M25/06 ,  C12M29/10

    Abstract: The invention is a flow-through bioreactor for the retention and culture of cells in perfused media. The bioreactor is a generally rectangular vessel with inlet and outlet ports in the lid allowing for media flow along the longitudinal axis of the vessel. The inner surface of the bottom wall of the bioreactor has a plurality of generally rectangular grooves having a length, a depth, and a width. The grooves are positioned in the bottom wall such that their length is transverse to the longitudinal axis of the vessel, allowing media flow across the width of the grooves. Cells settle into the grooves, where they proliferate and differentiate, without entering the bulk flow of media through the vessel, thus avoiding loss of cells due to media flow. The invention also provides a method for the perfusion culture of hematopoietic cells whereby a suspension of either unselected hematopoietic mononuclear cells or CD34+ selected cells is placed in the bioreactor and cultured without loss of non-adherent stem/progenitor cells. Various cytokines can be added to the culture medium such that the cells in the grooves form colony-forming units (CFU-GM, BFU-E, CFU-Mix), long-term culture initiating cells (LTC-IC), and granulocytic precursors (blast cells, promyelocytes, myelocytes, metamyelocytes).

    IN VITRO GROWTH OF NEUTROPHIL AND MEGAKARYOCYTE PRECURSORS IN SERUM-FREE MEDIA
    6.
    发明专利
    IN VITRO GROWTH OF NEUTROPHIL AND MEGAKARYOCYTE PRECURSORS IN SERUM-FREE MEDIA 未知

    公开(公告)号：CA2168091A1

    公开(公告)日：1995-03-02

    申请号：CA2168091

    申请日：1994-08-23

    Applicant: BAXTER INT

    Inventor： SMITH STEPHEN L ,  QIAO XIAOYING ,  MACIUKAS SUSAN M ,  BENDER JAMES G ,  VAN EPPS DENNIS E ,  LOUDOVARIS MAUREEN F

    IPC: C12Q1/06 ,  A61K35/12 ,  A61K35/14 ,  C12N5/078 ,  C12N5/0787 ,  C12N5/08 ,  C12N5/02

    Abstract: The invention provides serum-free, animal protein-free media formulations to be used in conjunction with hematopoietic growth factors for the in vitro growth of human neutrophil and megakaryocyte precursors. The medium is comprised of a base medium, corticosteriod, transferrin, insulin, cholesterol, ethanolamine, and human albumin. The invention also provides methods for preparing serum-free, animal protein-free suspensions of human hematopoietic precursor cells wherein the cellular component comprises at least about 16 % neutrophil precursors and at least about 1 % megakaryocyte precursors. Serum-free, animal protein-free suspensions of human hematopoietic cells are provided wherein the cellular component comprises at least about 30 %, preferably greater than 60 % neutrophil precursors. The neutrophil precursors are comprised of blast cells, promyelocytes, neutrophilic myelocytes, and neutrophilic metamyelocytes. Also provided are serum-free, animal protein-free cell suspensions wherein the cellular component comprises at least about 3 %, preferably greater than 8 % megakaryocyte precursors. Also provided are serum-free, animal-protein free cell suspensions wherein the cellular component comprises colony-forming units and cluster-forming units.

    HUMAN ERYTHROID PROGENITOR CELL POPULATION
    7.
    发明专利
    HUMAN ERYTHROID PROGENITOR CELL POPULATION 未知

    公开(公告)号：CA2160871A1

    公开(公告)日：1994-11-10

    申请号：CA2160871

    申请日：1994-04-14

    Applicant: BAXTER INT

    Inventor： BENDER JAMES G ,  VAN EPPS DENNIS E ,  UNVERZAGT KRISTEN

    IPC: A61K35/12 ,  A61K35/14 ,  A61K35/28 ,  A61K35/407 ,  A61K35/54 ,  A61P7/06 ,  C12N5/078 ,  C12N5/08 ,  C12Q1/02

    Abstract: The invention provides a method of preparing from hematopoietic cells a population of cells enriched for erythroid progenitor cells (BFU-E) in which up to 100% of the colony-forming units are BFU-E. The method entails separating hematopoietic progenitor cells on the basis of binding to a specific cell surface antigen such as CD34, and separation of erythroid progenitor cells based on binding to a lectin such as Ulex europaeus agglutinin. After separation of the erythroid progenitors, the lectin may be eluted off the cells by incubation with excess sugar, such as fucose, which competes for the cell-binding site on the lectin. The invention also provides a method of treatment for human patients having an erythroid disorder via administration of a cell population enriched for human erythroid progenitor cells. Also provided are diagnostic methods based on the enriched cell population.

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