公开(公告)号：WO1993022326A1

公开(公告)日：1993-11-11

申请号：PCT/US1993003337

申请日：1993-04-09

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  REDDY, Parameswara, Meda ,  HANNA, Naeem, Botros

IPC: C07H19/04

CPC classification number: C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  Y02P20/55

Abstract: Disclosed herein are protecting groups for exocyclic amino groups of the bases adenine, guanine and cytosine for use in the synthesis of oligonucleotides, the protecting groups being represented by the formula: -CO-(CH2)0-9-CH3. In a particularly preferred embodiment, the base cytosine is protected with acetyl (-CO-CH3), and the oligonucleotide incorporating said protected cytosine is subjected to a cleavage/deprotection reagent comprising at least one straight chain alkylamine having from 1 to about 10 carbon atoms.

Abstract translation: 本文公开了用于合成寡核苷酸的碱基腺嘌呤，鸟嘌呤和胞嘧啶的环外氨基的保护基团，保护基由式-CO-（CH 2）0-9-CH 3代表。 在特别优选的实施方案中，用乙酰基（-CO-CH 3）保护碱基胞嘧啶，并将含有所述被保护的胞嘧啶的寡核苷酸经受包含至少一个具有1至约10个碳原子的直链烷基胺的切割/去保护试剂 。

公开(公告)号：WO1993015395A1

公开(公告)日：1993-08-05

申请号：PCT/US1993000697

申请日：1993-01-25

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  GUTTMAN, Andras

IPC: G01N27/447

CPC classification number: G01N27/44747 ,  G01N27/44704

Abstract: Disclosed herein is a capillary column containing a dynamically cross-linked composition and method of use. In a particularly preferred embodiment, the dynamically cross-linked composition comprises: 1.0 % polyethylene oxide; 1.0 % polyethylene glycol; 1.0 % ethylene glycol; 100mM TRIS-CHES buffer; and 0.1 % sodium dodecyl sulphate, where the pH of the composition is between about 8.0 and about 9.0 and the viscosity of the composition is less than about 500 centipoise. The disclosed compositions can be used for the analysis of surfactant: proteinaceous material complexes and the generation of calibration curves over an extensive range of molecular weights, using capillary electrophoretic techniques. The disclosed compositions are particularly suited for capillary electrophoretic systems having UV-based detection.

公开(公告)号：WO1993005155A1

公开(公告)日：1993-03-18

申请号：PCT/US1992006380

申请日：1992-08-03

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  ADAMS, Craig, W.

IPC: C12N15/31

CPC classification number: C07K14/315 ,  Y10S530/825

Abstract: Disclosed herein are variant versions of Streptolysin O produced by recombinant DNA techniques. In an embodiment, the variant is soluble upon expression and has a specific hemolytic activity of about 14 hemolytic units per milligram.

Abstract translation: 本文公开了通过重组DNA技术产生的链球菌溶血素O的变体形式。 在一个实施方案中，变体在表达时可溶，并且具有每毫克约14个溶血单位的特异性溶血活性。

公开(公告)号：WO1992009580A1

公开(公告)日：1992-06-11

申请号：PCT/US1991006861

申请日：1991-09-20

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  REDDY, M., Parameswara ,  MICHAEL, Maged, Aziz ,  OH, Chan, S. ,  DOBASHI, Thomas, S. ,  GIRGIS, Nabih, S.

IPC: C07D219/04

CPC classification number: C07D231/12 ,  C07D219/04 ,  C07D221/12 ,  C07D233/56 ,  C07D249/08 ,  C07D401/06 ,  C07D405/12 ,  C07D409/12 ,  C07F9/64 ,  G01N33/582

Abstract: Novel chemiluminescent compounds are suitable for use in labeling biological molecules for assays such as immunoassays. These chemiluminescent labels are characterized by the incorporation of stable leaving groups. One class of these chemiluminescent labels comprises salts in which the leaving group contains a carboxyl carbon atom or its isoelectronic equivalent and a five-membered ring, including at least one heteroatom. The heteroatom is preferably oxygen or sulfur. This leaving group is linked to a positively charged moiety capable of producing light by chemiluminescence, which can be an acridinium, phenanthridinium, quinolinium, or benzacridinium moiety. Both the leaving group and the positively charged moiety can be substituted, such as with reactive substituents for covalently linking the label to a biological molecule. Substituents on the five-membered ring of the leaving group can form an additional ring. An additional class of these chemiluminescent labels comprises a chemical group that can produce light by chemiluminescence covalently linked to a leaving group comprising a moiety containing a sulfur, phosphorus, or carbon atom double-bonded to a more electronegative atom or atoms.

Abstract translation: 新型化学发光化合物适合用于标记生物分子用于测定如免疫测定。 这些化学发光标记的特征在于并入稳定的离去基团。 这些化学发光标记的一类包括其中离去基团含有羧基碳原子或其等电子等价物的盐和包含至少一个杂原子的五元环。 杂原子优选为氧或硫。 该离去基团与能够通过化学发光产生光的带正电荷的部分连接，其可以是吖啶鎓，菲啶鎓，喹啉鎓或苯并吖啶鎓部分。 离去基团和带正电荷的部分都可以被取代，例如用反应性取代基共价连接标记物与生物分子。 离去基团的五元环上的取代基可以形成另外的环。 这些化学发光标记的另外一类包括可以通过化学发光共价连接到离子基团上产生光的化学基团，所述离去基团包含与更多电负性原子或原子双键结合的含有硫，磷或碳原子的部分。

公开(公告)号：WO1992006383A1

公开(公告)日：1992-04-16

申请号：PCT/US1991006719

申请日：1991-09-17

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SHU, Frank, R.

IPC: G01N33/84

CPC classification number: G01N33/84

Abstract: Diluents and methodologies for determining the concentration of total calcium in a clinical sample (e.g. whole blood, sera plasma urine cerebro spinal fluid) containing protein-bound calcium are disclosed. Methodologies include the steps of: (a) admixing the sample with a diluent, the diluent including effective amounts of a pH buffer and at least two agents capable of complexing free calcium in the sample, where the agent-calcium complex association constants ("log K" in base 10) each have a range from about 1.5 to about 7.0; and (b) contacting an aliquot of the diluted sample with a calcium-specific ion selective electrode. The response of the calcium-specific ion selective electrode is an indication of the concentration of total calcium in the sample.

Abstract translation: 公开了用于确定含有蛋白质结合钙的临床样品（例如全血，血清尿脑脊髓液）中总钙浓度的稀释剂和方法。 方法包括以下步骤：（a）将样品与稀释剂混合，稀释剂包括有效量的pH缓冲剂和至少两种能够在样品中络合游离钙的试剂，其中试剂 - 钙络合物缔合常数（“log 基础10）中的“K”各自具有约1.5至约7.0的范围; 和（b）将稀释样品的等分试样与钙特异性离子选择性电极接触。 钙离子选择性电极的反应是样品中总钙浓度的指示。

公开(公告)号：WO1988009487A1

公开(公告)日：1988-12-01

申请号：PCT/US1988001428

申请日：1988-05-02

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  GIEBELER, Robert

IPC: G01J05/06

CPC classification number: G01J5/0022 ,  B04B15/02 ,  G01J5/06 ,  G01J5/08 ,  G01J5/0853 ,  G01J2005/0033 ,  G01J2005/126

Abstract: The isothermal radiometric system improved is of the double junction variety (preferably copper-constantan-copper) and includes a black body housing (B1) defining a central concavity. The central concavity has a black body disk (D1) at the bottom thereof. The disk is preferably suspended by a copper (14) and a constantan (16) wire with one junction (32) formed on the surface of the disk and the other junction (34) formed on the surface of the housing. The improvement includes a plurality of and preferably three annular baffles (50, 52, 54) defining central, circular and preferably concentric opening. These baffles are lodged in the opening (30) of the housing above the mounted radiometer disk (D1). The annular baffles on the side towards the radiation source are coated as a black body so as to increase the thermal coupling of the black body housing to the ambient being radiometrically observed. The baffles on the side away from the radiation source and exposed to the radiometer disk are reflectively coated to decrease further any couple between the radiometer disk and the black body housing. The combination of the baffles and the disk combine to give the radiometer a small field of view which permits radiometric thermal measurement of a small solid angle and ignores the remainder of the environment. Preferred use of the radiometer in looking at a centrifuge rotor (R1) is disclosed.

Abstract translation: 改进的等温辐射系统是双结品种（优选铜 - 康铜），并且包括限定中心凹部的黑体壳体（B1）。 中心凹部在其底部具有黑体盘（D1）。 盘优选地由铜（14）和康铜（16）线悬挂，其中一个结（32）形成在盘的表面上，而另一结（34）形成在壳体的表面上。 改进包括限定中心的，圆形的，优选地同心的开口的多个且优选三个环形挡板（50,52,54）。 这些挡板被放置在安装的辐射计盘（D1）上方的壳体的开口（30）中。 朝向辐射源的一侧上的环形挡板被涂覆为黑体，以便增加黑体壳体与被放射线观测的环境的热耦合。 远离辐射源并暴露于辐射计盘的一侧的挡板被反射涂覆，以进一步减少辐射计盘和黑体外壳之间的任何耦合。 挡板和盘的组合结合在一起使得辐射计具有小的视野，允许小的立体角的辐射热测量并且忽略其余的环境。 公开了辐射计在观察离心机转子（R1）中的优选用途。

公开(公告)号：WO1988009220A1

公开(公告)日：1988-12-01

申请号：PCT/US1988001429

申请日：1988-05-02

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  GIEBELER, Robert

IPC: B04B15/02

CPC classification number: B04B15/02 ,  F25B21/02 ,  F25B2321/023 ,  H01L2924/0002 ,  H01L2924/00

Abstract: A thermoelectric cooling design of the type having a nonconducting substrate for mounting thermoelectric coolers. Provisions for a flexible can (C) and heat dissipation from the thermoelectric coolers (K) are provided. For improved thermal response, the refrigerated vacuum can is provided with thin walls (18) of high purity aluminum with the result that deformation of the wall surface, especially the bottom wall surface can occur both with respect to installation. A plurality of can heat sinks (30) are placed on the bottom wall of the can to form a unitary and locally rigid side wall to the can at the point of attachment. At least one thermoelectric cooler (K) is communicated to the can heat sinks (10) at a first side for receiving heat energy from the can. A second discharge heat sink (22) is communicated to each thermoelectric cooler (K) for dissipating heat energy from both the can and the thermoelectric cooler. Spring biased connections (S) move the respective heat sinks towards one another and clamp the thermoelectric cooler firmly therebetween. The heat sinks dynamically conform to dimensional changes at the cooler interface during installation. The discharge heat sinks (22) are integrally cast with copper plugs placed in an aluminum mold and the aluminum cast about the copper plugs. The copper plug is preferably gold plated to prevent oxidation and form a heat conducting alloy with the aluminum. This enables heat transfer with a low temperature gradient to produce cooling with high efficiency.

公开(公告)号：WO1988009218A1

公开(公告)日：1988-12-01

申请号：PCT/US1988001427

申请日：1988-05-02

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  GIEBELER, Robert

IPC: B04B09/12

CPC classification number: B04B9/12 ,  Y10T74/2109

Abstract: In a damper for a centrifuge (10) for damping the rotor (16) of the centrifuge when the rotor changes rotational velocity through a critical vibrational rotation speed, an improved vibration damper is disclosed. The damper is of the type wherein a conically shaped shaft extension (50) is thrust into engagement with a friction bushing (40) at a circular and central opening to increase shaft section and shift the critical vibrational rotation speed away from the particular critical vibrational rotation speed being traversed. The conical bushing (40) is engaged by a solenoid (55) and translates side-to-side rotor motion to an energy dissipating up and down motion at the solenoid. The improvement disclosed is a conically shaped cone (50) having a negative radius of curvature (52) in section. For small shaft side-to-side excursion (due to small vibration) this conically shaped cone (50) has an initial small slope with respect to the bushing (40) to provide reduced damping of the rotor (16) when small vibration and hence small displacements effect the rotor. For large shaft side-to-side excurssion, this same conically shaped cone (50) has a large slope with respect to the low function bushing (40) which provides for increased displacement of the bushing (40) at large displacements of the rotor (16).

公开(公告)号：WO1988006296A1

公开(公告)日：1988-08-25

申请号：PCT/US1988000282

申请日：1988-02-01

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SHOPE, William, H.

IPC: G01T01/00

CPC classification number: G01T1/204 ,  G01N30/62 ,  G01N2030/77 ,  G01T1/003

Abstract: A flow cell (24) for liquid scintillation counting having a spool (35) wound with a length of light transparent tubing (40) provides a variable volume of sample solution for analysis by selectively winding the spool with a select length of tubing, thus controlling sensitivity of analysis and maintaining resolution of separated constituents in the sample. The flow cell comprises a spool (35) rotatably held within a framework (34) and which may be covered with a transparent material.

公开(公告)号：WO1988000706A1

公开(公告)日：1988-01-28

申请号：PCT/US1987001616

申请日：1987-07-08

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  BARKER, Stephen, F. ,  FECHTNER, Harold, F.

IPC: G01N35/04

CPC classification number: G01N35/04 ,  G01N2035/0451 ,  G01N2035/0465

Abstract: A sample loading and unloading apparatus for an automated clinical instrument including a loading tray (12) adapted to receive a plurality of sample carrying sectors (16). The apparatus further includes a sample carousel (14) having a plurality of locations each adapted to receive sample sectors. The portion of the sample carousel nearest the loading tray defines a transfer position (18). The loading tray includes a transfer assembly (20) for transferring a sector positioned at the transfer position from the sample carousel to the loading tray and simultaneously transferring a sector from the loading tray to the sample carousel. The transfer assembly includes a transfer spider (110) which may be pneumatically raised to temporarily lift the sample sectors from the loading tray and the sample sector from the transfer position. The transfer assembly is rotated to a next index position and is lowered to thereby transfer the sample sector from the transfer position to the loading tray, increment sample sectors around the loading tray, and transfer a sector from a loading position on the loading tray to the transfer position.

Abstract translation: 一种用于自动化临床仪器的样品装载和卸载装置，包括适于接收多个采样运送扇区（16）的装载托盘（12）。 该装置还包括具有多个位置的样本转盘（14），每个位置适于接收采样扇区。 最靠近装载托盘的样品转盘的部分限定了转移位置（18）。 装载托盘包括传送组件（20），用于将位于传送位置处的扇区从样品转盘传送到装载盘，同时将扇区从装载盘传送到样品转盘。 传送组件包括可以被气动地升高以将试样扇区从装载托盘和样品扇区临时提升到转移位置的转运支架（110）。 传送组件旋转到下一个分度位置并且被降低，从而将采样扇区从传送位置传送到装载盘，增加装载盘周围的采样扇区，并将扇区从装载盘上的装载位置传送到 转移位置。