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    CAPILLARY COLUMN CONTAINING A DYNAMICALLY CROSS-LINKED COMPOSITION AND METHOD OF USE
    1.
    发明申请
    CAPILLARY COLUMN CONTAINING A DYNAMICALLY CROSS-LINKED COMPOSITION AND METHOD OF USE 审中-公开
    包含动态交联组合物的毛细管柱和使用方法

    公开(公告)号：WO1993015395A1

    公开(公告)日：1993-08-05

    申请号：PCT/US1993000697

    申请日：1993-01-25

    Applicant: BECKMAN INSTRUMENTS, INC.

    Inventor： BECKMAN INSTRUMENTS, INC. ,  GUTTMAN, Andras

    IPC: G01N27/447

    CPC classification number: G01N27/44747 ,  G01N27/44704

    Abstract: Disclosed herein is a capillary column containing a dynamically cross-linked composition and method of use. In a particularly preferred embodiment, the dynamically cross-linked composition comprises: 1.0 % polyethylene oxide; 1.0 % polyethylene glycol; 1.0 % ethylene glycol; 100mM TRIS-CHES buffer; and 0.1 % sodium dodecyl sulphate, where the pH of the composition is between about 8.0 and about 9.0 and the viscosity of the composition is less than about 500 centipoise. The disclosed compositions can be used for the analysis of surfactant: proteinaceous material complexes and the generation of calibration curves over an extensive range of molecular weights, using capillary electrophoretic techniques. The disclosed compositions are particularly suited for capillary electrophoretic systems having UV-based detection.

    CAPILLARY ELECTROPHORESIS USING TIME-VARYING FIELD STRENGTH
    2.
    发明申请
    CAPILLARY ELECTROPHORESIS USING TIME-VARYING FIELD STRENGTH 审中-公开
    毛细管电泳使用时变场强

    公开(公告)号：WO1993025899A1

    公开(公告)日：1993-12-23

    申请号：PCT/US1993005809

    申请日：1993-06-17

    Applicant: BECKMAN INSTRUMENTS, INC.

    Inventor： BECKMAN INSTRUMENTS, INC. ,  GUTTMAN, Andras

    IPC: G01N27/447

    CPC classification number: G01N27/44773

    Abstract: The present invention is directed to a technique that provides enhanced separation resolution in electrophoresis. This technique involves the use of a time-varying field strength, which may be progressively increasing or decreasing, constant or otherwise, as a function of time. The shape of the field strength with respect to time may be continuous or stepwise over time, monotonic or otherwise. Because the mobility of different size species is a function of the applied electric field, the use of a nonuniform (time varying) electric field increases the resolving power of electrophoresis. This technique has been found to provide enhanced resolution of double-stranded DNA molecules in capillary polyacrylamide gel electrophoresis. It has been found that enhanced separation of DNA restriction fragments of 1 to more than 1000 base pairs in size has been achieved employing the technique of the present invention.

    CAPILLARY COLUMN CONTAINING REMOVABLE SEPARATION GEL COMPOSITION AND METHOD OF USE
    3.
    发明申请
    CAPILLARY COLUMN CONTAINING REMOVABLE SEPARATION GEL COMPOSITION AND METHOD OF USE 审中-公开
    包含可去除分离凝胶组成的毛细管柱及其使用方法

    公开(公告)号：WO1993015394A1

    公开(公告)日：1993-08-05

    申请号：PCT/US1993000650

    申请日：1993-01-25

    Applicant: BECKMAN INSTRUMENTS, INC.

    Inventor： BECKMAN INSTRUMENTS, INC. ,  GUTTMAN, Andras ,  SHIEH, Chia-Hui ,  KARGER, Barry, L. ,  PENTONEY, Stephen, L., Jr. ,  KONRAD, Kenneth, D. ,  RAMPAL, Sushma ,  GANZLER, Katalin

    IPC: G01N27/447

    CPC classification number: G01N27/44704 ,  G01N27/44747

    Abstract: Disclosed herein are capillary columns for capillary electrophoretic analysis of sample constituents. In an embodiment, the columns comprise a bifunctional layer comprising a positively charged amine group adsorbed to the inner wall of the capillary and an active functional group; a gel composition; hydrophilic polymer; and a separation composition capable of being removed from the column, interspersed within the remainder of the column. The disclosed columns are particularly well suited for the analysis of surfactant/proteinaceous material complexes, nucleic acid sequence, and analysis of restriction fragments according to size.

    Abstract translation: 本文公开了用于毛细管电泳分析样品成分的毛细管柱。 在一个实施方案中，柱包括双官能层，其包含吸附在毛细管内壁上的带正电胺基团和活性官能团; 凝胶组成; 亲水性聚合物; 以及能够从柱中除去，分散在塔的剩余部分内的分离组合物。 所公开的柱特别适用于根据尺寸分析表面活性剂/蛋白质物质复合物，核酸序列和限制性片段的分析。

    CAPILLARY COLUMN CONTAINING A DYNAMICALLY CROSS-LINKED COMPOSITION AND METHOD OF USE
    5.
    发明公开
    CAPILLARY COLUMN CONTAINING A DYNAMICALLY CROSS-LINKED COMPOSITION AND METHOD OF USE 失效
    含动态交联的组合物和使用方法毛细管柱。

    公开(公告)号：EP0578814A1

    公开(公告)日：1994-01-19

    申请号：EP93904645.0

    申请日：1993-01-25

    Applicant: BECKMAN INSTRUMENTS, INC.

    Inventor： GUTTMAN, Andras

    IPC: C08L71 ,  G01N27

    CPC classification number: G01N27/44747 ,  G01N27/44704

    Abstract: L'invention décrit une colonne capillaire contenant une composition réticulée dynamiquement, ainsi que le procédé d'utilisation correspondant. Dans un mode de réalisation particulièrement préféré, la composition réticulée dynamiquement comprend: 1,0 % d'oxyde de polyéthylène; 1,0 % de polyéthylène glycol; 1,0 % d'éthylène glycol; 100mM d'un tampon de TRIS-CHES; 0,1 % de dodécylsulfate de sodium, où le pH de la composition se situe entre 8,0 et 9,0 environ et la viscosité de ladite composition est inférieure à 500 centipoises. Les compositions décrites peuvent s'utiliser pour analyser des complexes constitués par un matériau tensioactif et protéinique, ainsi que dans la production de courbes d'étalonnage recouvrant une plage étendue de poids moléculaires, au moyen de techniques électrophorétiques capillaires. Les compositions décrites sont particulièrement appropriées pour des systèmes électrophorétiques capillaires possédant une détection à base d'ultraviolets.

    CAPILLARY COLUMN CONTAINING A DYNAMICALLY CROSS-LINKED COMPOSITION AND METHOD OF USE
    6.
    发明授权
    CAPILLARY COLUMN CONTAINING A DYNAMICALLY CROSS-LINKED COMPOSITION AND METHOD OF USE 失效
    含有利用动态交联的组合物和方法毛细管柱

    公开(公告)号：EP0578814B1

    公开(公告)日：1996-08-14

    申请号：EP93904645.4

    申请日：1993-01-25

    Applicant: BECKMAN INSTRUMENTS, INC.

    Inventor： GUTTMAN, Andras

    IPC: G01N27/447

    CPC classification number: G01N27/44747 ,  G01N27/44704

    Abstract: Disclosed herein is a capillary column containing a dynamically cross-linked composition and method of use. In a particularly preferred embodiment, the dynamically cross-linked composition comprises: 1.0 % polyethylene oxide; 1.0 % polyethylene glycol; 1.0 % ethylene glycol; 100mM TRIS-CHES buffer; and 0.1 % sodium dodecyl sulphate, where the pH of the composition is between about 8.0 and about 9.0 and the viscosity of the composition is less than about 500 centipoise. The disclosed compositions can be used for the analysis of surfactant: proteinaceous material complexes and the generation of calibration curves over an extensive range of molecular weights, using capillary electrophoretic techniques. The disclosed compositions are particularly suited for capillary electrophoretic systems having UV-based detection.

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