公开(公告)号：DE69837924T2

公开(公告)日：2007-10-18

申请号：DE69837924

申请日：1998-10-01

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A ,  BISHOP JAMES A ,  ABRAMS BARNABY

IPC: G01N33/532 ,  G01N33/533 ,  G01N33/58

Abstract: The inventors herein disclose new heterobifunctional chromophores that are capable of coupling with two distinct moieties. One moiety may be either a signal-enhancing agent or a blocking agent. The second moiety may be one member of a specific binding pair. The invention is based in part on the surprising result that when a chromophore is used as a "cross-linker" between a signal-enhancing agent and a member of a binding pair (essentially being buried between the two), the signal of the chromophore is not quenched. This arrangement, wherein the chromophore acts simultaneously as a cross-linker and a detectable compound, provides significant advantages over previously known compounds since the chromophore is sterically hindered from interacting non-specifically with substances present in the test systems. Moreover, the chromophore can be used as a cross-linker with little or no loss of detectable signal.

公开(公告)号：DE69626147T2

公开(公告)日：2003-10-23

申请号：DE69626147

申请日：1996-03-27

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A ,  BISHOP JAMES E

IPC: G01N15/10 ,  G01N15/14 ,  G01N33/58 ,  G01N33/96

Abstract: A rapid, simple method for preparing beads for calibrating flow cytometers which contain a known number of fluorophores per bead is presented. Briefly, the invention utilizes beads coated with a stable complex of a fluorophore and an enzyme. the enzymatic activity of a known number of beads gives an accurate measure of fluorophore density on those beads.

公开(公告)号：AU9402198A

公开(公告)日：1999-04-23

申请号：AU9402198

申请日：1998-09-21

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A ,  BISHOP JAMES E

IPC: A61K9/16 ,  A61K47/48 ,  G01N33/543

公开(公告)号：DE69115154T2

公开(公告)日：1996-07-04

申请号：DE69115154

申请日：1991-07-22

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A

IPC: G01N1/28 ,  C12N5/08 ,  G01N15/14 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/533 ,  G01N33/543 ,  G01N33/569 ,  G01N33/96 ,  G01N33/531

Abstract: Cells fixed with a fixative, reduced with a Schiff's base reducing agent and then dried in the presence of alpha - alpha -trehalose retain their light scatter and fluorescence properties and may be used, when rehydrated, as standards or controls in cellular analysis.

公开(公告)号：ES2083529T3

公开(公告)日：1996-04-16

申请号：ES91306643

申请日：1991-07-22

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A

IPC: G01N1/28 ,  C12N5/08 ,  G01N15/14 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/533 ,  G01N33/543 ,  G01N33/569 ,  G01N33/96 ,  G01N33/531

Abstract: Cells fixed with a fixative, reduced with a Schiff's base reducing agent and then dried in the presence of alpha - alpha -trehalose retain their light scatter and fluorescence properties and may be used, when rehydrated, as standards or controls in cellular analysis.

公开(公告)号：AU7724891A

公开(公告)日：1992-02-06

申请号：AU7724891

申请日：1991-05-22

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A ,  WARD ANTHONY J

IPC: G01N1/28 ,  C12N5/08 ,  G01N15/14 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/533 ,  G01N33/543 ,  G01N33/569 ,  G01N33/96 ,  G01N33/531 ,  G01N33/49

Abstract: Cells fixed with a fixative, reduced with a Schiff's base reducing agent and then dried in the presence of alpha - alpha -trehalose retain their light scatter and fluorescence properties and may be used, when rehydrated, as standards or controls in cellular analysis.

公开(公告)号：DE60219592T2

公开(公告)日：2007-12-27

申请号：DE60219592

申请日：2002-01-11

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A ,  WEI BING-YUAN

IPC: A61K39/00 ,  G01N33/53 ,  C07K1/14 ,  C07K14/435 ,  C07K14/47 ,  C07K14/705 ,  C07K14/74 ,  C07K19/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/63 ,  C12P21/04 ,  G01N21/78 ,  G01N33/543

Abstract: Presented are intrinsically fluorescent, self-multimerizing MHC fusion proteins, and complexes assembled therefrom that are capable of detectably labeling antigen-specific T lymphocytes. Also presented are methods for labeling antigen-specific T lymphocytes with the intrinsically fluorescent complexes of the present invention, and methods, particularly flow cytometric methods, for detecting, enumerating, enriching, and depleting antigen specific T lymphocytes so labeled.

公开(公告)号：DE60219592D1

公开(公告)日：2007-05-31

申请号：DE60219592

申请日：2002-01-11

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A ,  WEI BING-YUAN

IPC: A61K39/00 ,  G01N33/53 ,  C07K1/14 ,  C07K14/435 ,  C07K14/47 ,  C07K14/705 ,  C07K14/74 ,  C07K19/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/63 ,  C12P21/04 ,  G01N21/78 ,  G01N33/543

Abstract: Presented are intrinsically fluorescent, self-multimerizing MHC fusion proteins, and complexes assembled therefrom that are capable of detectably labeling antigen-specific T lymphocytes. Also presented are methods for labeling antigen-specific T lymphocytes with the intrinsically fluorescent complexes of the present invention, and methods, particularly flow cytometric methods, for detecting, enumerating, enriching, and depleting antigen specific T lymphocytes so labeled.

公开(公告)号：DE69115154D1

公开(公告)日：1996-01-18

申请号：DE69115154

申请日：1991-07-22

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A

IPC: G01N1/28 ,  C12N5/08 ,  G01N15/14 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/533 ,  G01N33/543 ,  G01N33/569 ,  G01N33/96 ,  G01N33/531

Abstract: Cells fixed with a fixative, reduced with a Schiff's base reducing agent and then dried in the presence of alpha - alpha -trehalose retain their light scatter and fluorescence properties and may be used, when rehydrated, as standards or controls in cellular analysis.

公开(公告)号：AT131283T

公开(公告)日：1995-12-15

申请号：AT91306643

申请日：1991-07-22

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A

IPC: G01N1/28 ,  C12N5/08 ,  G01N15/14 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/533 ,  G01N33/543 ,  G01N33/569 ,  G01N33/96 ,  G01N33/531

Abstract: Cells fixed with a fixative, reduced with a Schiff's base reducing agent and then dried in the presence of alpha - alpha -trehalose retain their light scatter and fluorescence properties and may be used, when rehydrated, as standards or controls in cellular analysis.