公开(公告)号：WO02055992A3

公开(公告)日：2003-03-27

申请号：PCT/US0200855

申请日：2002-01-11

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A ,  WEI BING-YUAN

IPC: G01N33/53 ,  C07K1/14 ,  C07K14/435 ,  C07K14/47 ,  C07K14/74 ,  C07K19/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  G01N21/78 ,  G01N33/543 ,  A61K39/00 ,  C12N15/63 ,  C12P21/04 ,  C12N115/85

CPC classification number: C07K14/70539 ,  C07K14/43595 ,  C07K19/00 ,  C07K2319/00 ,  Y10S435/975

Abstract: Presented are intrinsically fluorescent, self-multimerizing MHC fusion proteins, and complexes assembled therefrom that are capable of detectably labeling antigen-specific T lymphocytes. Also presented are methods for labeling antigen-specific T lymphocytes with the intrinsically fluorescent complexes of the present invention, and methods, particularly flow cytometric methods, for detecting, enumerating, enriching, and depleting antigen specific T lymphocytes so labeled.

Abstract translation: 本发明提供了本发明的荧光，自体多聚化MHC融合蛋白及其组装的复合体，其能够可检测地标记抗原特异性T淋巴细胞。 还提出了用本发明的本征荧光复合物标记抗原特异性T淋巴细胞的方法，以及用于检测，计数，富集和消耗如此标记的抗原特异性T淋巴细胞的方法，特别是流式细胞术方法。

公开(公告)号：WO0033082A3

公开(公告)日：2000-11-30

申请号：PCT/US9928884

申请日：1999-12-02

Applicant: BECTON DICKINSON CO ,  DAVIS KENNETH A

Inventor： DAVIS KENNETH A

IPC: G01N33/53 ,  C07K16/28 ,  G01N33/531 ,  G01N33/533 ,  G01N33/536 ,  G01N33/569 ,  G01N33/50

CPC classification number: G01N33/56977 ,  C07K16/2896 ,  G01N33/56972 ,  G01N2333/70539 ,  G01N2333/70553 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/975 ,  Y10S436/80 ,  Y10S436/808 ,  Y10S436/826

Abstract: Improved methods, reagents, and kits for quantitation of HLA-DR and/or CD11b expression on peripheral blood cells are presented. Inclusion of a lysosomotropic amine, such as chloroquine, during staining stabilizes HLA-DR and CD11b expression. Use of a novel anti-CD14 conjugate, anti-CD14-PerCP/CY5.5, permits the ready discrimination of monocytes. The improved methods, reagents, and kits can be used to assess immune competence, and to direct and monitor immunostimulatory therapies in patients exhibiting monocyte deactivation.

Abstract translation: 提出了用于定量外周血细胞上的HLA-DR和/或CD11b表达的改进的方法，试剂和试剂盒。 在染色期间包含溶酶体胺，如氯喹，稳定了HLA-DR和CD11b的表达。 使用新的抗CD14结合物，抗CD14-PerCP / CY5.5，可以准确鉴别单核细胞。 改进的方法，试剂和试剂盒可用于评估免疫能力，并指导和监测显示单核细胞失活的患者的免疫刺激疗法。

公开(公告)号：IE76319B1

公开(公告)日：1997-10-22

申请号：IE173491

申请日：1991-05-21

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A ,  WARD ANTHONY J

IPC: G01N1/28 ,  C12N5/08 ,  G01N15/14 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/533 ,  G01N33/543 ,  G01N33/569 ,  G01N33/96 ,  G01N33/531

Abstract: Cells fixed with a fixative, reduced with a Schiff's base reducing agent and then dried in the presence of alpha - alpha -trehalose retain their light scatter and fluorescence properties and may be used, when rehydrated, as standards or controls in cellular analysis.

公开(公告)号：AU645807B2

公开(公告)日：1994-01-27

申请号：AU7724891

申请日：1991-05-22

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A ,  WARD ANTHONY J

IPC: G01N1/28 ,  C12N5/08 ,  G01N15/14 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/533 ,  G01N33/543 ,  G01N33/569 ,  G01N33/96 ,  G01N33/531 ,  G01N33/49 ,  C12N5/02

Abstract: Cells fixed with a fixative, reduced with a Schiff's base reducing agent and then dried in the presence of alpha - alpha -trehalose retain their light scatter and fluorescence properties and may be used, when rehydrated, as standards or controls in cellular analysis.

公开(公告)号：CA2043045A1

公开(公告)日：1992-01-24

申请号：CA2043045

申请日：1991-05-22

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A ,  WARD ANTHONY J

IPC: G01N1/28 ,  C12N5/08 ,  G01N15/14 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/533 ,  G01N33/543 ,  G01N33/569 ,  G01N33/96

Abstract: Cells fixed with a fixative, reduced with a Schiff's base reducing agent and then dried in the presence of .alpha.-.alpha.-trehalose retain their light scatter and fluorescence properties and may be used, when rehydrated, as standards or controls in cellular analysis.

公开(公告)号：DE69837924D1

公开(公告)日：2007-07-26

申请号：DE69837924

申请日：1998-10-01

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A ,  BISHOP JAMES A ,  ABRAMS BARNABY

IPC: G01N33/532 ,  G01N33/533 ,  G01N33/58

Abstract: The inventors herein disclose new heterobifunctional chromophores that are capable of coupling with two distinct moieties. One moiety may be either a signal-enhancing agent or a blocking agent. The second moiety may be one member of a specific binding pair. The invention is based in part on the surprising result that when a chromophore is used as a "cross-linker" between a signal-enhancing agent and a member of a binding pair (essentially being buried between the two), the signal of the chromophore is not quenched. This arrangement, wherein the chromophore acts simultaneously as a cross-linker and a detectable compound, provides significant advantages over previously known compounds since the chromophore is sterically hindered from interacting non-specifically with substances present in the test systems. Moreover, the chromophore can be used as a cross-linker with little or no loss of detectable signal.

公开(公告)号：AT359812T

公开(公告)日：2007-05-15

申请号：AT02713396

申请日：2002-01-11

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A ,  WEI BING-YUAN

IPC: G01N33/53 ,  C07K1/14 ,  C07K14/435 ,  C07K14/47 ,  C07K14/74 ,  C07K19/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  G01N21/78 ,  G01N33/543 ,  A61K39/00 ,  C12N15/63 ,  C12P21/04 ,  C07K14/705

Abstract: Presented are intrinsically fluorescent, self-multimerizing MHC fusion proteins, and complexes assembled therefrom that are capable of detectably labeling antigen-specific T lymphocytes. Also presented are methods for labeling antigen-specific T lymphocytes with the intrinsically fluorescent complexes of the present invention, and methods, particularly flow cytometric methods, for detecting, enumerating, enriching, and depleting antigen specific T lymphocytes so labeled.

公开(公告)号：DE69626147D1

公开(公告)日：2003-03-20

申请号：DE69626147

申请日：1996-03-27

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A ,  BISHOP JAMES E

IPC: G01N15/10 ,  G01N15/14 ,  G01N33/58 ,  G01N33/96

Abstract: A rapid, simple method for preparing beads for calibrating flow cytometers which contain a known number of fluorophores per bead is presented. Briefly, the invention utilizes beads coated with a stable complex of a fluorophore and an enzyme. the enzymatic activity of a known number of beads gives an accurate measure of fluorophore density on those beads.

公开(公告)号：AU9681498A

公开(公告)日：1999-04-27

申请号：AU9681498

申请日：1998-10-01

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A ,  BISHOP JAMES A JR ,  ABRAMS BARNABY

IPC: G01N33/532 ,  G01N33/533 ,  G01N33/58

Abstract: The inventors herein disclose new heterobifunctional chromophores that are capable of coupling with two distinct moieties. One moiety may be either a signal-enhancing agent or a blocking agent. The second moiety may be one member of a specific binding pair. The invention is based in part on the surprising result that when a chromophore is used as a "cross-linker" between a signal-enhancing agent and a member of a binding pair (essentially being buried between the two), the signal of the chromophore is not quenched. This arrangement, wherein the chromophore acts simultaneously as a cross-linker and a detectable compound, provides significant advantages over previously known compounds since the chromophore is sterically hindered from interacting non-specifically with substances present in the test systems. Moreover, the chromophore can be used as a cross-linker with little or no loss of detectable signal.

公开(公告)号：EP1349569A4

公开(公告)日：2005-01-26

申请号：EP02713396

申请日：2002-01-11

Applicant: BECTON DICKINSON CO

Inventor： DAVIS KENNETH A ,  WEI BING-YUAN

IPC: G01N33/53 ,  C07K1/14 ,  C07K14/435 ,  C07K14/47 ,  C07K14/74 ,  C07K19/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  G01N21/78 ,  G01N33/543 ,  A61K39/00 ,  C07K14/705 ,  C12N15/63 ,  C12P21/04

CPC classification number: C07K14/70539 ,  C07K14/43595 ,  C07K19/00 ,  C07K2319/00 ,  Y10S435/975