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公开(公告)号：AU2018239659A1
公开(公告)日：2019-10-31
申请号：AU2018239659
申请日：2018-03-26
Applicant: HARVARD COLLEGE , MASSACHUSETTS GEN HOSPITAL
Inventor： CHOU DAVID BENSON , LEIJTEN LILIANA S TEIXEIRA MOREIRA , RECH ARIANNA , NOVAK RICHARD , INGBER DONALD E , MILTON YUKA , FRISMANTAS VIKTORAS , LEVY OREN
Abstract: The present disclosure relates to a microfluidic devices and methods for culturing bone marrow cells. Aspects include methods of preparing microfluidic devices and culturing bone marrow cells with the microfluidic devices. In some aspects, a method includes providing a microfluidic device having an upper chamber, a lower chamber, and a porous membrane separating the upper chamber from the lower chamber. The method further includes seeding walls of the lower chamber and a bottom surface of the membrane with endothelial cells. The method further includes providing a matrix within the upper chamber. The matrix includes fibrin gel and bone marrow cells. The method further includes filling or perfusing the upper chamber with a media.
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12.发明专利
公开(公告)号：AU2018224829A1
公开(公告)日：2019-08-29
申请号：AU2018224829
申请日：2018-02-26
Applicant: HARVARD COLLEGE
Inventor： MAOZ BEN M , HERLAND ANNA , HENRY OLIVIER F , PARKER KEVIN K , INGBER DONALD E
Abstract: An organ-on-chip device for monitoring a biological function and including a membrane layer located at an interface region between a top microchannel and the a microchannel. The membrane includes a first type of cells forming a barrier between the top microchannel and the bottom microchannel. The device further includes a top layer having a first plurality of transendothelial electrical resistance (TEER) measurement electrodes for enabling direct monitoring of cell function and electrical activity of the first type of cells on the membrane. The device also has a multi-electrode array (MEA) layer with a second plurality of TEER measurement electrodes for enabling direct monitoring of cell function and electrical activity of a second type of cells on the MEA layer.
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公开(公告)号：AU2017298298A1
公开(公告)日：2019-02-21
申请号：AU2017298298
申请日：2017-07-18
Applicant: HARVARD COLLEGE
Inventor： GOYAL GIRIJA , INGBER DONALD E
Abstract: An organ-on-a-chip microfluidic device is disclosed that mimics a human lymph node and/or human lymphoid tissue. The device can include cells from human blood and lymphatic tissue, include an extracellular matrix for the development of immune system components, and provide for the perfusion of fluids and solids resembling blood and lymphatic fluid within micrometer sized channels.
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公开(公告)号：AU2017268248A1
公开(公告)日：2018-12-13
申请号：AU2017268248
申请日：2017-05-16
Applicant: HARVARD COLLEGE
Inventor： LESLIE DANIEL CHRISTOPHER , DOYLE THOMAS , WATERHOUSE ANNA , RODAS MELISSA , WATTERS ALEXANDER L , SUPER MICHAEL , INGBER DONALD E
IPC: C07K17/08
Abstract: This disclosure provides, e.g., methods for coupling an entity to a solid substrate. The method can comprise treating the substrate with a plasma, e.g., a CO2 plasma, to increase its reactivity. The entity can be, e.g., a biological polymer that binds a microbe. Substrates produced by these methods can be used in a variety of applications, including hemodialysis and diagnostic assays.
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15.发明专利
公开(公告)号：ES2678143T3
公开(公告)日：2018-08-09
申请号：ES11735056
申请日：2011-01-19
Applicant: HARVARD COLLEGE
Inventor： SUPER MICHAEL , WAY JEFFREY , INGBER DONALD E
Abstract: Proteína de fusión de opsonina recombinante que comprende: un dominio de reconocimiento de carbohidrato de una lectina de unión a manosa (MBL) en donde el dominio de reconocimiento de carbohidrato permite la unión a manosa en la superficie de un microbio condensado a una porción Fc de una inmunoglobulina, en donde la proteína de fusión de opsonina recombinante excluye un dominio funcional de la opsonina que se une a una serina proteasa asociada a lectina de unión a manano (MASP).
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Patent Agency Ranking16.发明专利
公开(公告)号：AU2016382614A1
公开(公告)日：2018-07-19
申请号：AU2016382614
申请日：2016-12-02
Applicant: HARVARD COLLEGE
Inventor： HAJIPOURAN BENAM KAMBEZ , NOVAK RICHARD , INGBER DONALD E
Abstract: A device is directed to simulating a function of a tissue, and includes a first structure defining a first chamber, a second structure defining a second chamber, and a porous membrane located at an interface region between the first chamber and the second chamber. The membrane has a first side facing toward the first chamber and a second side facing toward the second chamber, the membrane separating the first chamber from the second chamber. The first side includes a fluid-permeable, stimulus-responsive polymer gel thereon, the second side including at least one layer of cells adhered thereon.
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公开(公告)号：CA3036378A1
公开(公告)日：2018-03-22
申请号：CA3036378
申请日：2017-09-13
Applicant: HARVARD COLLEGE
Abstract: Described herein are methods for providing an in vitro intestinal model system, e.g., using primary cells instead of cell lines and/or cancerous cells.
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公开(公告)号：AU2017254907A1
公开(公告)日：2017-11-23
申请号：AU2017254907
申请日：2017-11-01
Applicant: HARVARD COLLEGE
Inventor： ROTTMAN MARTIN M , INGBER DONALD E , SUPER MICHAEL , WAY JEFFREY CHARLES , CARTWRIGHT MARK J , WATTERS ALEXANDER , BERTHET JULIA B , SUPER DINAH R
IPC: C07K14/47 , C07K14/705 , G01N33/543 , G01N33/569
Abstract: [005841 Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.
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公开(公告)号：CA3005332A1
公开(公告)日：2017-08-03
申请号：CA3005332
申请日：2016-11-14
Applicant: HARVARD COLLEGE
Inventor： OLIVER JOHN W K , NOVAK RICHARD , CRONCE MICHAEL J , INGBER DONALD E , WAY JEFFREY C
Abstract: A low-oxygen system is directed to culturing a diverse microbiome and includes an anaerobic chamber and at least one microfluidic device removably inserted in the anaerobic chamber. The microfluidic device includes a first microchannel in which a first level of oxygen is maintained, and a second microchannel in which a second level of oxygen is maintained, the second level of oxygen having a greater oxygen concentration than the first level of oxygen. The microfluidic device further includes a membrane located at an interface region between the first microchannel and the second microchannel, the membrane further having a plurality of pores via which oxygen flows from the second microchannel to the first microchannel to form an oxygen gradient in the first microchannel. The system further includes a culture system provided in the first microchannel and including oxygen-sensitive anaerobic bacteria.
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20.发明专利
公开(公告)号：AU2015353853A1
公开(公告)日：2017-06-15
申请号：AU2015353853
申请日：2015-11-19
Applicant: HARVARD COLLEGE
Inventor： INGBER DONALD E , MUSAH SAMIRA
Abstract: Embodiments of various aspects described herein relate to methods, kits, and cell culture media for generation of podocytes from pluripotent stem (PS) cells, as well as cells produced by the same, and methods of use.
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